pAAV-CMV-DIO-Blank-SV40-GFP

                            
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        659..963
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        964..1167
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    complement(1192..1225)
                     /bound_moiety="Cre recombinase"
                     /note="lox2272"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
                     /note="color: #31849b; direction: LEFT"
     protein_bind    complement(1288..1321)
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: LEFT"
     protein_bind    1355..1388
                     /bound_moiety="Cre recombinase"
                     /note="lox2272"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
                     /note="color: #31849b; direction: RIGHT"
     protein_bind    1459..1492
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: RIGHT"
     rep_origin      1698..1833
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     polyA_signal    2605..2726
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(3102..3690)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             4497..5291
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc
      481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg
      541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta
      601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg
      661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc
      961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg
     1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc
     1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta
     1321 tacgcgtctc gagtcagata tcgctagcca attgataact tcgtatagga tactttatac
     1381 gaagttatca ttgggattct tcctattttg atccaagcat caccatcgac cctctagtcc
     1441 agatctcacc atcgacccat aacttcgtat agcatacatt atacgaagtt atatggtgag
     1501 caagggcgat tctagagttt aaacacgcgt ggtgtggaaa gtccccaggc tccccagcag
     1561 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     1621 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     1681 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     1741 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     1801 tccagaagta gtgaggaggc ttttttggag gccgccacca tggagagcga cgagagcggc
     1861 ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag
     1921 ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa gatgaagagc
     1981 accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc
     2041 taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac
     2101 ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc
     2161 ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc
     2221 ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag
     2281 cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg
     2341 cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc
     2401 caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg
     2461 cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc
     2521 ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc
     2581 tccaccggat ctcgctgact taagaacttg tttattgcag cttataatgg ttacaaataa
     2641 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     2701 ttgtccaaac tcatcaatgt atcttaaagc ttaggaaccc ctagtgatgg agttggccac
     2761 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     2821 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agggatcccg gtgtgaaata
     2881 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     2941 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     3001 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     3061 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3121 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3181 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3241 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3301 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3361 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3421 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3481 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3541 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3601 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3661 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3721 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3781 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     3841 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     3901 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     3961 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     4021 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     4081 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4141 ccagttaata gtttgcgcaa cgttgttgaa aaaggatctt cacctagatc cttttcacgt
     4201 agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct
     4261 ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc
     4321 gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc
     4381 cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttctcg ccgccaagga
     4441 tctgatggcg caggggatca agctctgatc aagagacagg atgaggatcg tttcgcatga
     4501 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     4561 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     4621 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     4681 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     4741 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     4801 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     4861 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     4921 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     4981 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
     5041 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     5101 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     5161 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     5221 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     5281 agttcttctg aattttgtta aaatttttgt taaatcagct cattttttaa ccaataggcc
     5341 gaaatcggca acatccctta taaatcaaaa gaatagaccg cgatagggtt gagtgttgtt
     5401 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     5461 accgtctatc agggcgatgg cccactacgt gaaccatcac ccaaatcaag ttttttgcgg
     5521 tcgaggtgcc gtaaagctct aaatcggaac cctaaaggga gcccccgatt tagagcttga
     5581 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     5641 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc gcgcttaatg
     5701 cgccgctaca gggcgcgtcc attcgccatt caggatcgaa ttaattctta attaacatca
     5761 tcaataatat acctt
//