pAAV-CMV-DIO-Blank-SV40-Puro

                            LOCUS       dna                      5798 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    457..462
                     /dbxref="REBASE:HpaI"
     misc_binding    457..462
                     /dbxref="REBASE:HincII"
     misc_binding    833..838
                     /dbxref="REBASE:NdeI"
     misc_binding    1168..1173
                     /dbxref="REBASE:KpnI"
     Other Gene      1288..1321
                     /gene="loxP other"
     misc_binding    1322..1327
                     /dbxref="REBASE:XhoI"
     misc_binding    1331..1336
                     /dbxref="REBASE:EcoRV"
     misc_binding    1435..1440
                     /dbxref="REBASE:BglII"
     Other Gene      1453..1486
                     /gene="loxP other"
     misc_binding    1505..1510
                     /dbxref="REBASE:XbaI"
     Promoter        1639..1907
                     /gene="SV40 prom"
     Rep_Origin      1806..1883
                     /gene="SV40 origin"
     ORF             1873..2541
                     /sequence="ORF_1 rf(1)"
     misc_binding    1886..1898
                     /dbxref="REBASE:SfiI"
     Marker          1942..2541
                     /gene="puro marker"
     misc_binding    2886..2891
                     /dbxref="REBASE:BamHI"
     Rep_Origin      3110..3729
                     /gene="pBR322 origin"
     Promoter        4382..4431
                     /gene="NEOKAN prom"
     misc_binding    4489..4494
                     /dbxref="REBASE:BclI"
     ORF             4520..5314
                     /sequence="ORF_2 rf(2)"
     Marker          4523..5311
                     /gene="NTP_II marker"
     Rep_Origin      5419..5724
                     /gene="f1 origin"
BASE COUNT    1359 a   1547 c   1583 g   1309 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc     
      481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg     
      541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta     
      601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg     
      661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt     
      721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca     
      781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc     
      841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta     
      901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc     
      961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga     
     1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg     
     1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta     
     1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg     
     1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc     
     1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta     
     1321 tctcgagtca gatatcgcta gccaattgat aacttcgtat aggatacttt atacgaagtt     
     1381 atcattggga ttcttcctat tttgatccaa gcatcaccat cgaccctcta gtccagatct     
     1441 caccatcgac ccataacttc gtatagcata cattatacga agttatatgg tgagcaaggg     
     1501 cgattctaga aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac     
     1561 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat     
     1621 caatgtatct taaagcttgg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc     
     1681 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag     
     1741 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc     
     1801 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa     
     1861 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt     
     1921 gaggaggctt ttttggaggc catgaccgag tacaagccca cggtgcgcct cgccacccgc     
     1981 gacgacgtcc ctcgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg     
     2041 cgccacaccg tggacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc     
     2101 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg     
     2161 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg     
     2221 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga agggctcctg     
     2281 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac     
     2341 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc     
     2401 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg     
     2461 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg     
     2521 acccgcaagc ccggtgcctg actgtgcctt ctagttgcca gccatctgtt gtttgcccct     
     2581 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg     
     2641 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc     
     2701 aggacagcaa gggggaggat tgggaagaga atagcaggca tgctggggaa agcttaggaa     
     2761 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg     
     2821 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg     
     2881 cgcagggatc ccggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc     
     2941 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg     
     3001 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa     
     3061 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg     
     3121 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga     
     3181 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg     
     3241 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg     
     3301 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc     
     3361 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg     
     3421 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca     
     3481 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt     
     3541 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag     
     3601 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg     
     3661 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc     
     3721 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt     
     3781 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt     
     3841 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca     
     3901 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg     
     3961 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac     
     4021 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg     
     4081 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc     
     4141 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gaaaaaggat     
     4201 cttcacctag atccttttca cgtagaaagc cagtccgcag aaacggtgct gaccccggat     
     4261 gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt     
     4321 agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga     
     4381 accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg     
     4441 gatggctttc tcgccgccaa ggatctgatg gcgcagggga tcaagctctg atcaagagac     
     4501 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc     
     4561 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc     
     4621 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc     
     4681 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg     
     4741 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt     
     4801 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc     
     4861 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga     
     4921 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga     
     4981 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct     
     5041 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc     
     5101 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt     
     5161 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg     
     5221 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat     
     5281 cgccttctat cgccttcttg acgagttctt ctgaattttg ttaaaatttt tgttaaatca     
     5341 gctcattttt taaccaatag gccgaaatcg gcaacatccc ttataaatca aaagaataga     
     5401 ccgcgatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg     
     5461 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat     
     5521 cacccaaatc aagttttttg cggtcgaggt gccgtaaagc tctaaatcgg aaccctaaag     
     5581 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga     
     5641 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa     
     5701 ccaccacacc cgcgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggatc     
     5761 gaattaattc ttaattaaca tcatcaataa tatacctt                             
//