pAAV-CMV-DIO-GFP
| Vector Name | pAAV-CMV-DIO-GFP |
| VectorType | AAV Vector |
| Antibiotic Information | Kanamycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | No Additional Information |
Double-click on the features for more information
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc 481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg 541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta 601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg 661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc 961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg 1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc 1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta 1321 tctcgagcaa gtttgtacaa aaaagcaggc ttcagcgaga tccggtggag ccgggtccgg 1381 cggtgccgtc cacggcagaa ttggacgact gagcgcggga tctggcgaag gcgatggggg 1441 tcttgaaggc gtgctggtac tccacgatgc ccagctcggt gttgctgtgc agctcctcca 1501 cgcggcggaa ggcgaacatg gggcccccgt tctgcaggat gctggggtgg atggcgctct 1561 tgaagtgcat gtggctgtcc accacgaagc tgtagtagcc gccgtcgcgc aggctgaagg 1621 tgcgggcgaa gctgcccacc agcacgttat cgcccatggg gtgcaggtgc tccacggtgg 1681 cgttgctgcg gatgatcttg tcggtgaaga tcacgctgtc ctcggggaag ccggtgccca 1741 ccaccttgaa gtcgccgatc acgcggccgg cctcgtagcg gtagctgaag ctcacgtgca 1801 gcacgccgcc gtcctcgtac ttctcgatgc gggtgttggt gtagccgccg ttgttgatgg 1861 cgtgcaggaa ggggttctcg tagccgctgg ggtaggtgcc gaagtggtag aagccgtagc 1921 ccatcacgtg gctcagcagg taggggctga aggtcagggc gcctttggtg ctcttcatct 1981 tgttggtcat gcggccctgc ttgggggtgc cctctccgcc gcccaccagc tcgaactcca 2041 cgccgttcag ggtgccggtg atgcggcact cgatctccat ggcgggcagg ccgctctcgt 2101 cgctctccat acccagcttt cttgtacaaa gtgggctagc caattgataa cttcgtatag 2161 gatactttat acgaagttat cattgggatt cttcctattt tgatccaagc atcaccatcg 2221 accctctagt ccagatctca ccatcgaccc ataacttcgt atagcataca ttatacgaag 2281 ttatatggtg agcaagggcg attctagaaa cttgtttatt gcagcttata atggttacaa 2341 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 2401 tggtttgtcc aaactcatca atgtatctta aagcttagga acccctagtg atggagttgg 2461 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac 2521 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagggat cccggtgtga 2581 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct 2641 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 2701 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 2761 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 2821 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 2881 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 2941 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 3001 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 3061 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 3121 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 3181 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 3241 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 3301 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 3361 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 3421 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 3481 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 3541 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 3601 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 3661 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 3721 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 3781 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 3841 ttcgccagtt aatagtttgc gcaacgttgt tgaaaaagga tcttcaccta gatccttttc 3901 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct 3961 atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca 4021 tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg 4081 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt ctcgccgcca 4141 aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc 4201 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc 4261 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca 4321 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg 4381 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg 4441 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag 4501 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg 4561 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc 4621 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa 4681 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac 4741 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat 4801 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac 4861 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc 4921 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt 4981 gacgagttct tctgaatttt gttaaaattt ttgttaaatc agctcatttt ttaaccaata 5041 ggccgaaatc ggcaacatcc cttataaatc aaaagaatag accgcgatag ggttgagtgt 5101 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg 5161 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcacccaaat caagtttttt 5221 gcggtcgagg tgccgtaaag ctctaaatcg gaaccctaaa gggagccccc gatttagagc 5281 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg 5341 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgcgcgctt 5401 aatgcgccgc tacagggcgc gtccattcgc cattcaggat cgaattaatt cttaattaac 5461 atcatcaata atatacctt
LOCUS dna 5479 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 457..462 /dbxref="REBASE:HincII" misc_binding 833..838 /dbxref="REBASE:NdeI" misc_binding 1168..1173 /dbxref="REBASE:KpnI" Other Gene 1288..1321 /gene="loxP other" ORF 1301..2203 /sequence="ORF_2 rf(2)" misc_binding 1322..1327 /dbxref="REBASE:XhoI" ORF 1352..2110 /sequence="ORF_3 rf(4)" misc_binding 1521..1526 /dbxref="REBASE:ApaI" misc_binding 1532..1537 /dbxref="REBASE:PstI" misc_binding 2233..2238 /dbxref="REBASE:BglII" Other Gene 2251..2284 /gene="loxP other" misc_binding 2303..2308 /dbxref="REBASE:XbaI" misc_binding 2431..2436 /dbxref="REBASE:HindIII" misc_binding 2567..2572 /dbxref="REBASE:BamHI" Rep_Origin 2791..3410 /gene="pBR322 origin" Promoter 4063..4112 /gene="NEOKAN prom" misc_binding 4170..4175 /dbxref="REBASE:BclI" ORF 4201..4995 /sequence="ORF_1 rf(1)" Marker 4204..4992 /gene="NTP_II marker" misc_binding 4730..4735 /dbxref="REBASE:SphI" Rep_Origin 5100..5405 /gene="f1 origin" BASE COUNT 1284 a 1398 c 1521 g 1276 t 0 others ORIGIN 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc 481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg 541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta 601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg 661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc 961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg 1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc 1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta 1321 tctcgagcaa gtttgtacaa aaaagcaggc ttcagcgaga tccggtggag ccgggtccgg 1381 cggtgccgtc cacggcagaa ttggacgact gagcgcggga tctggcgaag gcgatggggg 1441 tcttgaaggc gtgctggtac tccacgatgc ccagctcggt gttgctgtgc agctcctcca 1501 cgcggcggaa ggcgaacatg gggcccccgt tctgcaggat gctggggtgg atggcgctct 1561 tgaagtgcat gtggctgtcc accacgaagc tgtagtagcc gccgtcgcgc aggctgaagg 1621 tgcgggcgaa gctgcccacc agcacgttat cgcccatggg gtgcaggtgc tccacggtgg 1681 cgttgctgcg gatgatcttg tcggtgaaga tcacgctgtc ctcggggaag ccggtgccca 1741 ccaccttgaa gtcgccgatc acgcggccgg cctcgtagcg gtagctgaag ctcacgtgca 1801 gcacgccgcc gtcctcgtac ttctcgatgc gggtgttggt gtagccgccg ttgttgatgg 1861 cgtgcaggaa ggggttctcg tagccgctgg ggtaggtgcc gaagtggtag aagccgtagc 1921 ccatcacgtg gctcagcagg taggggctga aggtcagggc gcctttggtg ctcttcatct 1981 tgttggtcat gcggccctgc ttgggggtgc cctctccgcc gcccaccagc tcgaactcca 2041 cgccgttcag ggtgccggtg atgcggcact cgatctccat ggcgggcagg ccgctctcgt 2101 cgctctccat acccagcttt cttgtacaaa gtgggctagc caattgataa cttcgtatag 2161 gatactttat acgaagttat cattgggatt cttcctattt tgatccaagc atcaccatcg 2221 accctctagt ccagatctca ccatcgaccc ataacttcgt atagcataca ttatacgaag 2281 ttatatggtg agcaagggcg attctagaaa cttgtttatt gcagcttata atggttacaa 2341 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 2401 tggtttgtcc aaactcatca atgtatctta aagcttagga acccctagtg atggagttgg 2461 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac 2521 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagggat cccggtgtga 2581 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct 2641 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 2701 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 2761 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 2821 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 2881 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 2941 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 3001 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 3061 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 3121 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 3181 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 3241 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 3301 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 3361 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 3421 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 3481 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 3541 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 3601 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 3661 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 3721 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 3781 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 3841 ttcgccagtt aatagtttgc gcaacgttgt tgaaaaagga tcttcaccta gatccttttc 3901 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct 3961 atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca 4021 tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg 4081 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt ctcgccgcca 4141 aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc 4201 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc 4261 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca 4321 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg 4381 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg 4441 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag 4501 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg 4561 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc 4621 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa 4681 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac 4741 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat 4801 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac 4861 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc 4921 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt 4981 gacgagttct tctgaatttt gttaaaattt ttgttaaatc agctcatttt ttaaccaata 5041 ggccgaaatc ggcaacatcc cttataaatc aaaagaatag accgcgatag ggttgagtgt 5101 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg 5161 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcacccaaat caagtttttt 5221 gcggtcgagg tgccgtaaag ctctaaatcg gaaccctaaa gggagccccc gatttagagc 5281 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg 5341 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgcgcgctt 5401 aatgcgccgc tacagggcgc gtccattcgc cattcaggat cgaattaatt cttaattaac 5461 atcatcaata atatacctt //