pAAV-CMV-DIO-GFP-SV40-Puro

                            LOCUS       dna                      6596 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    457..462
                     /dbxref="REBASE:HpaI"
     misc_binding    457..462
                     /dbxref="REBASE:HincII"
     misc_binding    833..838
                     /dbxref="REBASE:NdeI"
     misc_binding    1168..1173
                     /dbxref="REBASE:KpnI"
     Other Gene      1288..1321
                     /gene="loxP other"
     ORF             1301..2203
                     /sequence="ORF_2 rf(2)"
     misc_binding    1322..1327
                     /dbxref="REBASE:XhoI"
     ORF             1352..2110
                     /sequence="ORF_4 rf(5)"
     misc_binding    1521..1526
                     /dbxref="REBASE:ApaI"
     misc_binding    1532..1537
                     /dbxref="REBASE:PstI"
     misc_binding    2233..2238
                     /dbxref="REBASE:BglII"
     Other Gene      2251..2284
                     /gene="loxP other"
     misc_binding    2303..2308
                     /dbxref="REBASE:XbaI"
     Promoter        2437..2705
                     /gene="SV40 prom"
     Rep_Origin      2604..2681
                     /gene="SV40 origin"
     ORF             2671..3339
                     /sequence="ORF_1 rf(1)"
     misc_binding    2684..2696
                     /dbxref="REBASE:SfiI"
     Marker          2740..3339
                     /gene="puro marker"
     misc_binding    3684..3689
                     /dbxref="REBASE:BamHI"
     Rep_Origin      3908..4527
                     /gene="pBR322 origin"
     Promoter        5180..5229
                     /gene="NEOKAN prom"
     misc_binding    5287..5292
                     /dbxref="REBASE:BclI"
     ORF             5318..6112
                     /sequence="ORF_3 rf(2)"
     Marker          5321..6109
                     /gene="NTP_II marker"
     Rep_Origin      6217..6522
                     /gene="f1 origin"
BASE COUNT    1482 a   1778 c   1864 g   1472 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc     
      481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg     
      541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta     
      601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg     
      661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt     
      721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca     
      781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc     
      841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta     
      901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc     
      961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga     
     1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg     
     1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta     
     1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg     
     1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc     
     1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta     
     1321 tctcgagcaa gtttgtacaa aaaagcaggc ttcagcgaga tccggtggag ccgggtccgg     
     1381 cggtgccgtc cacggcagaa ttggacgact gagcgcggga tctggcgaag gcgatggggg     
     1441 tcttgaaggc gtgctggtac tccacgatgc ccagctcggt gttgctgtgc agctcctcca     
     1501 cgcggcggaa ggcgaacatg gggcccccgt tctgcaggat gctggggtgg atggcgctct     
     1561 tgaagtgcat gtggctgtcc accacgaagc tgtagtagcc gccgtcgcgc aggctgaagg     
     1621 tgcgggcgaa gctgcccacc agcacgttat cgcccatggg gtgcaggtgc tccacggtgg     
     1681 cgttgctgcg gatgatcttg tcggtgaaga tcacgctgtc ctcggggaag ccggtgccca     
     1741 ccaccttgaa gtcgccgatc acgcggccgg cctcgtagcg gtagctgaag ctcacgtgca     
     1801 gcacgccgcc gtcctcgtac ttctcgatgc gggtgttggt gtagccgccg ttgttgatgg     
     1861 cgtgcaggaa ggggttctcg tagccgctgg ggtaggtgcc gaagtggtag aagccgtagc     
     1921 ccatcacgtg gctcagcagg taggggctga aggtcagggc gcctttggtg ctcttcatct     
     1981 tgttggtcat gcggccctgc ttgggggtgc cctctccgcc gcccaccagc tcgaactcca     
     2041 cgccgttcag ggtgccggtg atgcggcact cgatctccat ggcgggcagg ccgctctcgt     
     2101 cgctctccat acccagcttt cttgtacaaa gtgggctagc caattgataa cttcgtatag     
     2161 gatactttat acgaagttat cattgggatt cttcctattt tgatccaagc atcaccatcg     
     2221 accctctagt ccagatctca ccatcgaccc ataacttcgt atagcataca ttatacgaag     
     2281 ttatatggtg agcaagggcg attctagaaa cttgtttatt gcagcttata atggttacaa     
     2341 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg     
     2401 tggtttgtcc aaactcatca atgtatctta aagcttggtg tggaaagtcc ccaggctccc     
     2461 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt     
     2521 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca     
     2581 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc     
     2641 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg     
     2701 agctattcca gaagtagtga ggaggctttt ttggaggcca tgaccgagta caagcccacg     
     2761 gtgcgcctcg ccacccgcga cgacgtccct cgggccgtac gcaccctcgc cgccgcgttc     
     2821 gccgactacc ccgccacgcg ccacaccgtg gacccggacc gccacatcga gcgggtcacc     
     2881 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg     
     2941 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg     
     3001 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa     
     3061 cagatggaag ggctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc     
     3121 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga     
     3181 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac     
     3241 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga     
     3301 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac tgtgccttct agttgccagc     
     3361 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg     
     3421 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc     
     3481 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagagaat agcaggcatg     
     3541 ctggggaaag cttaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc     
     3601 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc     
     3661 tcagtgagcg agcgagcgcg cagggatccc ggtgtgaaat accgcacaga tgcgtaagga     
     3721 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg     
     3781 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat     
     3841 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta     
     3901 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa     
     3961 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc     
     4021 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt     
     4081 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca     
     4141 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg     
     4201 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat     
     4261 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta     
     4321 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct     
     4381 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac     
     4441 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa     
     4501 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa     
     4561 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt     
     4621 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca     
     4681 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca     
     4741 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc     
     4801 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa     
     4861 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc     
     4921 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca     
     4981 acgttgttga aaaaggatct tcacctagat ccttttcacg tagaaagcca gtccgcagaa     
     5041 acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag     
     5101 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt     
     5161 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa     
     5221 gccctgcaaa gtaaactgga tggctttctc gccgccaagg atctgatggc gcaggggatc     
     5281 aagctctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca     
     5341 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac     
     5401 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt     
     5461 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc     
     5521 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg     
     5581 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc     
     5641 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc     
     5701 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat     
     5761 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc     
     5821 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca     
     5881 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga     
     5941 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat     
     6001 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc     
     6061 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaattttgtt     
     6121 aaaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aacatccctt     
     6181 ataaatcaaa agaatagacc gcgatagggt tgagtgttgt tccagtttgg aacaagagtc     
     6241 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg     
     6301 gcccactacg tgaaccatca cccaaatcaa gttttttgcg gtcgaggtgc cgtaaagctc     
     6361 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg     
     6421 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag     
     6481 cggtcacgct gcgcgtaacc accacacccg cgcgcttaat gcgccgctac agggcgcgtc     
     6541 cattcgccat tcaggatcga attaattctt aattaacatc atcaataata tacctt         
//