pAAV-CMV-circ-Control
| Vector Name | pAAV-CMV-circ-Control |
| Additional Information | No Additional Information |
Double-click on the features for more information
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcctgcggccgcacgcgtgtgtctagggttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagagagctagcTTTCTTAATCATCTGAAGCATGGAGTTTTAAAACATTTCAATTCAACAAATGTTAACTACTGCTTGTCCTAGAAGATACAAGGATGAATAACACATGGACCCCACCCTTAATAACTATGACGTATCTATGATTGATAGATGTTGACAACCAAAAGACGGGAACTATTAATTCTGTTGGGAGCATGGGGCGAAGAATAATTCAAAAATTCATAAAGAAGTAACATCTGAATTAGGTCCTGGAGGATAAACAGGTAGTTACTAGAATGAAAAGAATGGAAAGGTACTTAAGAGACCAAGGACCAGAATAAACTAAAACAACAGGGAGCAAAGCACAGATTGTATTTGGGGAACACCCAGCTATAGAACAAGATTGAGAAGGAGGCAGAAAAATAGCCCAGCTTCACATAGTAAGGTCAGATTACATTAAATTTCAAATGGCTTTGCAATATAAGGAATCATAAAACCCCCCCAAAAGAAATAAAGTAATTCTCAATTTGAGATAAAAAGCAATTATTTTTATGCTGTATAAAATTTCATCAGTTAAGAACTGTATCTCTCACCCACTAGGAAAAATAAAAGGAAGTTAATAGAACAAAGATTTCACCTAACCATCAAATGGACTAGAAAGTCTTTAGCAATTACTGTATTTTGATCATGAGAAAAGACGTAATTGCTGCCTATTTCATTTTAAATATGATCAATTTTTCCACTCATATAAACATATCAGAATATATAACCTATATATAATCTTTCTGTTTAGGAACAAAATCTAAGTCCAttaattaagaattcgtcgacgatatctggatcctctagaGGCGCGCCTGGACTTAGATTTTGTTCCTAAACAGAAAGATTATATATAGGTTATATATTCTGATATGTTTATATGAGTGGAAAAATTGATCATATTTAAAATGAAATAGGCAGCAATTACGTCTTTTCTCATGATCAAAATACAGTAATTGCTAAAGACTTTCTAGTCCATTTGATGGTTAGGTGAAATCTTTGTTCTATTAACTTCCTTTTATTTTTCCTAGTGGGTGAGAGATACAGTTCTTAACTGATGAAATTTTATACAGCATAAAAATAATTGCTTTTTATCTCAAATTGAGAATTACTTTATTTCTTTTGGGGGGGTTTTATGATTCCTTATATTGCAAAGCCATTTGAAATTTAATGTAATCTGACCTTACTATGTGAAGCTGGGCTATTTTTCTGCCTCCTTCTCAATCTTGTTCTATAGCTGGGTGTTCCCCAAATACAATCTGTGCTTTGCTCCCTGTTGTTTTAGTTTATTCTGGTCCTTGGTCTCTTAAGTACCTTTCCATTCTTTTCATTCTAGTAACTACCTGTTTATCCTCCAGGACCTAATTCAGATGTTACTTCTTTATGAATTTTTGAATTATTCTTCGCCCCATGCTCCCAACAGAATTAATAGTTCCCGTCTTTTGGTTGTCAACATCTATCAATCATAGATACGTCATAGTTATTAAGGGTGGGGTCCATGTGTTATTCATCCTTGTATCTTCTAGGACAAGCAGTAGTTAACATTTGTTGAATTGAAATGTTTTAAAACTCCATGCTTCAGATGATTAAGAAActcgagCTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGgaattcgtgcggaccgagcggccgcaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccttagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaactctatctcgggctattcttttgatttataagggattttgccgatttcggtctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgt
LOCUS Exported File 5437 bp ds-DNA circular SYN 18-JUL-2024
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS pAAV-CMV-circ-Control
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5437)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Feb-25-25 from SnapGene 1.1.3
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5437
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 1..130
/note="AAV2 ITR (alternate)"
/note="color: #ffcc99"
enhancer 160..462
/note="CMV enhancer"
/note="color: #ffffff"
promoter 463..666
/note="CMV promoter"
/note="color: #ffffff; direction: RIGHT"
misc_feature 789..1566
/note="intron F"
/note="color: #ccffcc"
misc_feature 1614..2391
/note="intron RC"
/note="color: #339966"
polyA_signal 2398..2622
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
/note="color: #a6acb3"
repeat_region 2648..2788
/note="AAV2 ITR"
/note="color: #ffe4c4"
rep_origin 2863..3318
/direction=RIGHT
/note="f1 ori"
/note="color: #ffff00"
promoter 3652..3756
/note="AmpR promoter"
/note="color: #ffffff; direction: RIGHT"
CDS 3757..4617
/codon_start=1
/note="AmpR"
/note="This feature has 2 segments:
1: 3757 .. 3825 / #ccffcc / signal sequence
2: 3826 .. 4617 / #ccffcc"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4788..5376
/direction=RIGHT
/note="ori"
/note="color: #ffff00"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtgtct agggttccgc gttacataac ttacggtaaa
181 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
241 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
301 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
361 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
421 tacttggcag tacatctacg tttagtcatc gctattacca tggtgatgcg gttttggcag
481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
661 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
721 catagaagaa ccgagtttaa actccctatc agtgatagag atctccctat cagtgataga
781 gagctagctt tcttaatcat ctgaagcatg gagttttaaa acatttcaat tcaacaaatg
841 ttaactactg cttgtcctag aagatacaag gatgaataac acatggaccc cacccttaat
901 aactatgacg tatctatgat tgatagatgt tgacaaccaa aagacgggaa ctattaattc
961 tgttgggagc atggggcgaa gaataattca aaaattcata aagaagtaac atctgaatta
1021 ggtcctggag gataaacagg tagttactag aatgaaaaga atggaaaggt acttaagaga
1081 ccaaggacca gaataaacta aaacaacagg gagcaaagca cagattgtat ttggggaaca
1141 cccagctata gaacaagatt gagaaggagg cagaaaaata gcccagcttc acatagtaag
1201 gtcagattac attaaatttc aaatggcttt gcaatataag gaatcataaa acccccccaa
1261 aagaaataaa gtaattctca atttgagata aaaagcaatt atttttatgc tgtataaaat
1321 ttcatcagtt aagaactgta tctctcaccc actaggaaaa ataaaaggaa gttaatagaa
1381 caaagatttc acctaaccat caaatggact agaaagtctt tagcaattac tgtattttga
1441 tcatgagaaa agacgtaatt gctgcctatt tcattttaaa tatgatcaat ttttccactc
1501 atataaacat atcagaatat ataacctata tataatcttt ctgtttagga acaaaatcta
1561 agtccattaa ttaagaattc gtcgacgata tctggatcct ctagaggcgc gcctggactt
1621 agattttgtt cctaaacaga aagattatat ataggttata tattctgata tgtttatatg
1681 agtggaaaaa ttgatcatat ttaaaatgaa ataggcagca attacgtctt ttctcatgat
1741 caaaatacag taattgctaa agactttcta gtccatttga tggttaggtg aaatctttgt
1801 tctattaact tccttttatt tttcctagtg ggtgagagat acagttctta actgatgaaa
1861 ttttatacag cataaaaata attgcttttt atctcaaatt gagaattact ttatttcttt
1921 tgggggggtt ttatgattcc ttatattgca aagccatttg aaatttaatg taatctgacc
1981 ttactatgtg aagctgggct atttttctgc ctccttctca atcttgttct atagctgggt
2041 gttccccaaa tacaatctgt gctttgctcc ctgttgtttt agtttattct ggtccttggt
2101 ctcttaagta cctttccatt cttttcattc tagtaactac ctgtttatcc tccaggacct
2161 aattcagatg ttacttcttt atgaattttt gaattattct tcgccccatg ctcccaacag
2221 aattaatagt tcccgtcttt tggttgtcaa catctatcaa tcatagatac gtcatagtta
2281 ttaagggtgg ggtccatgtg ttattcatcc ttgtatcttc taggacaagc agtagttaac
2341 atttgttgaa ttgaaatgtt ttaaaactcc atgcttcaga tgattaagaa actcgagctg
2401 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
2461 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
2521 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
2581 aagacaatag caggcatgct ggggatgcgg tgggctctat gggaattcgt gcggaccgag
2641 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
2701 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
2761 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
2821 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
2881 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct
2941 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
3001 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
3061 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
3121 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
3181 gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
3241 cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
3301 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
3361 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
3421 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
3481 caccgtcatc accgaaacgc gcgagccggg agctgcatgt gtcagaggtt ttcaccgtca
3541 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
3601 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
3661 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
3721 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
3781 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
3841 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
3901 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
3961 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
4021 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
4081 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
4141 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
4201 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
4261 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
4321 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
4381 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
4441 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
4501 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
4561 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
4621 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
4681 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
4741 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
4801 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
4861 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
4921 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
4981 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
5041 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
5101 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
5161 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
5221 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
5281 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
5341 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
5401 cggttcctgg ccttttgctg gccttttgct cacatgt
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