pAAV-G-CMV-DIO-SV40-GFP
| Vector Name | pAAV-G-CMV-DIO-SV40-GFP |
| VectorType | AAV Vector |
| Antibiotic Information | Kanamycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | No Additional Information |
Double-click on the features for more information
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgtgggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaacacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacaggaagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagtaagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttgtgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgttaacctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttccttgtagttaatgattaacccgccatgctacttatctacgtagccatgctctaggaagatcgcctaggcgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctggtaccaatcagctcgctcaccatataacttcgtataaagtatcctatacgaagttatatcaaaataggaagaccaatgcttcaccatcgacccgaattgccaagcatcaccatcgacccataacttcgtataatgtatgctatacgaagttatacgcgtctcgagtcagatatcatgatatcgctagccaattgataacttcgtataggatactttatacgaagttatcattgggattcttcctattttgatccaagcatcaccatcgaccctctagtccagatctcaccatcgacccataacttcgtatagcatacattatacgaagttatatggtgagcaagggcgattctagagtttaaacacgcgtggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggccgccaccatggagagcgacgagagcggcctgcccgccatggagatcgagtgccgcatcaccggcaccctgaacggcgtggagttcgagctggtgggcggcggagagggcacccccaagcagggccgcatgaccaacaagatgaagagcaccaaaggcgccctgaccttcagcccctacctgctgagccacgtgatgggctacggcttctaccacttcggcacctaccccagcggctacgagaaccccttcctgcacgccatcaacaacggcggctacaccaacacccgcatcgagaagtacgaggacggcggcgtgctgcacgtgagcttcagctaccgctacgaggccggccgcgtgatcggcgacttcaaggtggtgggcaccggcttccccgaggacagcgtgatcttcaccgacaagatcatccgcagcaacgccaccgtggagcacctgcaccccatgggcgataacgtgctggtgggcagcttcgcccgcaccttcagcctgcgcgacggcggctactacagcttcgtggtggacagccacatgcacttcaagagcgccatccaccccagcatcctgcagaacgggggccccatgttcgccttccgccgcgtggaggagctgcacagcaacaccgagctgggcatcgtggagtaccagcacgccttcaagacccccatcgccttcgccagatcccgcgctcagtcgtccaattctgccgtggacggcaccgccggacccggctccaccggatctcgctgacttaagaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttaaagcttaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagggatcccggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactggatggctttctcgccgccaaggatctgatggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttgttaaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatcccttataaatcaaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggatcgaattaattcttaattaacatcatcaataatatacctt
FEATURES Location/Qualifiers
source 1..5783
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 659..963
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
/note="color: #ffffff"
promoter 964..1167
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
/note="color: #ffffff; direction: RIGHT"
protein_bind complement(1192..1225)
/bound_moiety="Cre recombinase"
/note="lox2272"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GGATACTT). lox2272 sites are compatible with each
other, but incompatible with loxP or loxN sites."
/note="color: #31849b; direction: LEFT"
protein_bind complement(1288..1321)
/bound_moiety="Cre recombinase"
/note="loxP"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
/note="color: #31849b; direction: LEFT"
protein_bind 1363..1396
/bound_moiety="Cre recombinase"
/note="lox2272"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GGATACTT). lox2272 sites are compatible with each
other, but incompatible with loxP or loxN sites."
/note="color: #31849b; direction: RIGHT"
protein_bind 1467..1500
/bound_moiety="Cre recombinase"
/note="loxP"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
/note="color: #31849b; direction: RIGHT"
rep_origin 1706..1841
/note="SV40 ori"
/note="SV40 origin of replication"
/note="color: #ffff00"
polyA_signal 2613..2734
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
/note="color: #a6acb3"
rep_origin complement(3110..3698)
/direction=LEFT
/note="ori"
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
CDS 4505..5299
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
/note="color: #ccffcc"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc
481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg
541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta
601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg
661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc
961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg
1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc
1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta
1321 tacgcgtctc gagtcagata tcatgatatc gctagccaat tgataacttc gtataggata
1381 ctttatacga agttatcatt gggattcttc ctattttgat ccaagcatca ccatcgaccc
1441 tctagtccag atctcaccat cgacccataa cttcgtatag catacattat acgaagttat
1501 atggtgagca agggcgattc tagagtttaa acacgcgtgg tgtggaaagt ccccaggctc
1561 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
1621 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
1681 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
1741 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
1801 tgagctattc cagaagtagt gaggaggctt ttttggaggc cgccaccatg gagagcgacg
1861 agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg aacggcgtgg
1921 agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg accaacaaga
1981 tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac gtgatgggct
2041 acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc ctgcacgcca
2101 tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc ggcgtgctgc
2161 acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg
2221 gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc agcaacgcca
2281 ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc gcccgcacct
2341 tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg cacttcaaga
2401 gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg
2461 aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc ttcaagaccc
2521 ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg
2581 gacccggctc caccggatct cgctgactta agaacttgtt tattgcagct tataatggtt
2641 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
2701 gttgtggttt gtccaaactc atcaatgtat cttaaagctt aggaacccct agtgatggag
2761 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
2821 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ggatcccggt
2881 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
2941 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
3001 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
3061 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
3121 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
3181 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
3241 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
3301 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
3361 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
3421 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
3481 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
3541 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
3601 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
3661 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
3721 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
3781 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
3841 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
3901 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
3961 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
4021 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
4081 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
4141 gtagttcgcc agttaatagt ttgcgcaacg ttgttgaaaa aggatcttca cctagatcct
4201 tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg tcagctactg
4261 ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt gcagtgggct
4321 tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg aattgccagc
4381 tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg ctttctcgcc
4441 gccaaggatc tgatggcgca ggggatcaag ctctgatcaa gagacaggat gaggatcgtt
4501 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
4561 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
4621 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
4681 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
4741 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
4801 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
4861 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
4921 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
4981 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
5041 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
5101 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
5161 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
5221 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
5281 tcttgacgag ttcttctgaa ttttgttaaa atttttgtta aatcagctca ttttttaacc
5341 aataggccga aatcggcaac atcccttata aatcaaaaga atagaccgcg atagggttga
5401 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
5461 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc aaatcaagtt
5521 ttttgcggtc gaggtgccgt aaagctctaa atcggaaccc taaagggagc ccccgattta
5581 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
5641 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgcgc
5701 gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggatcgaatt aattcttaat
5761 taacatcatc aataatatac ctt
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