pAAV-G-CMV-DIO-SV40-Puro

                            LOCUS       dna                      7511 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    457..462
                     /dbxref="REBASE:HpaI"
     misc_binding    833..838
                     /dbxref="REBASE:NdeI"
     misc_binding    1168..1173
                     /dbxref="REBASE:KpnI"
     Other Gene      1288..1321
                     /gene="loxP other"
     misc_binding    1322..1327
                     /dbxref="REBASE:XhoI"
     Other Gene      1343..1462
                     /gene="attR1 other"
     Other Gene      1362..1462
                     /gene="attR2 other"
     misc_binding    1469..1474
                     /dbxref="REBASE:PstI"
     Other Gene      1503..1808
                     /gene="ccdB other"
     Marker          2150..2809
                     /gene="CAT marker"
     ORF             2150..2809
                     /sequence="ORF_3 rf(5)"
     misc_binding    2591..2596
                     /dbxref="REBASE:EcoRI"
     misc_binding    2910..2917
                     /dbxref="REBASE:NotI"
     Other Gene      2918..3042
                     /gene="attR1 other"
     Other Gene      2918..3018
                     /gene="attR2 other"
     Other Gene      3166..3199
                     /gene="loxP other"
     misc_binding    3218..3223
                     /dbxref="REBASE:XbaI"
     Promoter        3352..3620
                     /gene="SV40 prom"
     Rep_Origin      3519..3596
                     /gene="SV40 origin"
     ORF             3586..4254
                     /sequence="ORF_1 rf(1)"
     misc_binding    3599..3611
                     /dbxref="REBASE:SfiI"
     Marker          3655..4254
                     /gene="puro marker"
     Rep_Origin      4823..5442
                     /gene="pBR322 origin"
     Promoter        6095..6144
                     /gene="NEOKAN prom"
     misc_binding    6202..6207
                     /dbxref="REBASE:BclI"
     ORF             6233..7027
                     /sequence="ORF_2 rf(2)"
     Marker          6236..7024
                     /gene="NTP_II marker"
     Rep_Origin      7132..7437
                     /gene="f1 origin"
BASE COUNT    1839 a   1949 c   1930 g   1793 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc     
      481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg     
      541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta     
      601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg     
      661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt     
      721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca     
      781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc     
      841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta     
      901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc     
      961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga     
     1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg     
     1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta     
     1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg     
     1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc     
     1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta     
     1321 tctcgagtca gatatcaacc actttgtaca agaaagctga acgagaaacg taaaatgata     
     1381 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa     
     1441 cacaacatat ccagtcacta tggtcgacct gcagactggc tgtgtataag ggagcctgac     
     1501 atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg     
     1561 agatcagcca cttcttcccc gataacggag accggcacac tggccatatc ggtggtcatc     
     1621 atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga gactttatct     
     1681 gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata     
     1741 tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta     
     1801 aactgcattt caccagcccc tgttctcgtc agcaaaagag ccgttcattt caataaaccg     
     1861 ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac gcagacgacg     
     1921 ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat atatgccttg     
     1981 agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat agcatacctc     
     2041 tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa aaatcagcgc     
     2101 gcaaatacgc atactgttat ctggctttta gtaagccgga tccagatctt tacgccccgc     
     2161 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat     
     2221 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat     
     2281 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat     
     2341 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc     
     2401 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta     
     2461 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct     
     2521 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca     
     2581 ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg     
     2641 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa     
     2701 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat     
     2761 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct     
     2821 tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac gagccggaag     
     2881 cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga tatgttgtgt     
     2941 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat     
     3001 atcattttac gtttctcgtt cagctttttt gtacaaactt gttgatatcg ctagccaatt     
     3061 gataacttcg tataggatac tttatacgaa gttatcattg ggattcttcc tattttgatc     
     3121 caagcatcac catcgaccct ctagtccaga tctcaccatc gacccataac ttcgtatagc     
     3181 atacattata cgaagttata tggtgagcaa gggcgattct agaaacttgt ttattgcagc     
     3241 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc     
     3301 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaagct tggtgtggaa     
     3361 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa     
     3421 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca     
     3481 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca     
     3541 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg     
     3601 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggccatgacc     
     3661 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccctcgggc cgtacgcacc     
     3721 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtggaccc ggaccgccac     
     3781 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc     
     3841 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc     
     3901 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg     
     3961 ctggccgcgc agcaacagat ggaagggctc ctggcgccgc accggcccaa ggagcccgcg     
     4021 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc     
     4081 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc     
     4141 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc     
     4201 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgactgtgc     
     4261 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag     
     4321 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta     
     4381 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag     
     4441 agaatagcag gcatgctggg gaaagcttag gaacccctag tgatggagtt ggccactccc     
     4501 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc     
     4561 tttgcccggg cggcctcagt gagcgagcga gcgcgcaggg atcccggtgt gaaataccgc     
     4621 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact     
     4681 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac     
     4741 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa     
     4801 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg     
     4861 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa     
     4921 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc     
     4981 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac     
     5041 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac     
     5101 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg     
     5161 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt     
     5221 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga     
     5281 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct     
     5341 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga     
     5401 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg     
     5461 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct     
     5521 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt     
     5581 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc     
     5641 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg     
     5701 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag     
     5761 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt     
     5821 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag     
     5881 ttaatagttt gcgcaacgtt gttgaaaaag gatcttcacc tagatccttt tcacgtagaa     
     5941 agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac     
     6001 aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata     
     6061 gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc     
     6121 tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttctcgccgc caaggatctg     
     6181 atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc gcatgattga     
     6241 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga     
     6301 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg     
     6361 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga     
     6421 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt     
     6481 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct     
     6541 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct     
     6601 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg     
     6661 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca     
     6721 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga     
     6781 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt     
     6841 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt     
     6901 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct     
     6961 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt     
     7021 cttctgaatt ttgttaaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa     
     7081 tcggcaacat cccttataaa tcaaaagaat agaccgcgat agggttgagt gttgttccag     
     7141 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg     
     7201 tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt ttgcggtcga     
     7261 ggtgccgtaa agctctaaat cggaacccta aagggagccc ccgatttaga gcttgacggg     
     7321 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg     
     7381 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgcgcgc ttaatgcgcc     
     7441 gctacagggc gcgtccattc gccattcagg atcgaattaa ttcttaatta acatcatcaa     
     7501 taatatacct t                                                          
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