pAdeno-hTERT

                            LOCUS       dna                      8456 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    665..670
                     /dbxref="REBASE:NruI"
     misc_binding    942..947
                     /dbxref="REBASE:NdeI"
     Regulatory_Seq  1279..1318
                     /gene="tetO reg"
     ORF             1306..2127
                     /sequence="ORF_4 rf(6)"
     misc_binding    1409..1414
                     /dbxref="REBASE:HindIII"
     misc_binding    1415..1420
                     /dbxref="REBASE:KpnI"
     misc_binding    1450..1455
                     /dbxref="REBASE:EcoRI"
     promoter        1456..5170
                     /gene="hTERT"
     ORF             1513..4911
                     /sequence="ORF_1 rf(1)"
     ORF             2548..3768
                     /sequence="ORF_3 rf(6)"
     misc_binding    5196..5203
                     /dbxref="REBASE:NotI"
     misc_binding    5209..5214
                     /dbxref="REBASE:XbaI"
     misc_binding    5495..5500
                     /dbxref="REBASE:ClaI"
     selectable_marker661..6441
                     /gene="Kan/Neo"
     Rep_Origin      5768..6387
                     /gene="pBR322 origin"
     Promoter        7040..7089
                     /gene="NEOKAN prom"
     ORF             7178..7972
                     /sequence="ORF_2 rf(2)"
     Marker          7181..7969
                     /gene="NTP_II marker"
     Rep_Origin      8077..8382
                     /gene="f1 origin"
BASE COUNT    1748 a   2431 c   2491 g   1786 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtaa gctcagatct ggatctcccg     
      481 atcccctatg gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc     
      541 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa     
      601 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc     
      661 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat     
      721 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac     
      781 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa     
      841 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt     
      901 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc     
      961 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat     
     1021 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc     
     1081 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc     
     1141 tccaccccat tgacgtcaat gggagtttgt tttggaacca aaatcaacgg gactttccaa     
     1201 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg     
     1261 tctatataag cagagctctc cctatcagtg atagagatct ccctatcagt gatagagatc     
     1321 gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg     
     1381 acctccatag aagctagcgt ttaaacttaa gcttggtacc gagctcggat ccactagtcc     
     1441 agtgtggtgg aattcaggca gcgctgcgtc ctgctgcgca cgtgggaagc cctggccccg     
     1501 gccacccccg cgatgccgcg cgctccccgc tgccgagccg tgcgctccct gctgcgcagc     
     1561 cactaccgcg aggtgctgcc gctggccacg ttcgtgcggc gcctggggcc ccagggctgg     
     1621 cggctggtgc agcgcgggga cccggcggct ttccgcgcgc tggtggccca gtgcctggtg     
     1681 tgcgtgccct gggacgcacg gccgcccccc gccgccccct ccttccgcca ggtgtcctgc     
     1741 ctgaaggagc tggtggcccg agtgctgcag aggctgtgcg agcgcggcgc gaagaacgtg     
     1801 ctggccttcg gcttcgcgct gctggacggg gcccgcgggg gcccccccga ggccttcacc     
     1861 accagcgtgc gcagctacct gcccaacacg gtgaccgacg cactgcgggg gagcggggcg     
     1921 tgggggctgc tgctgcgccg cgtgggcgac gacgtgctgg ttcacctgct ggcacgctgc     
     1981 gcgctctttg tgctggtggc tcccagctgc gcctaccagg tgtgcgggcc gccgctgtac     
     2041 cagctcggcg ctgccactca ggcccggccc ccgccacacg ctagtggacc ccgaaggcgt     
     2101 ctgggatgcg aacgggcctg gaaccatagc gtcagggagg ccggggtccc cctgggcctg     
     2161 ccagccccgg gtgcgaggag gcgcgggggc agtgccagcc gaagtctgcc gttgcccaag     
     2221 aggcccaggc gtggcgctgc ccctgagccg gagcggacgc ccgttgggca ggggtcctgg     
     2281 gcccacccgg gcaggacgcg tggaccgagt gaccgtggtt tctgtgtggt gtcacctgcc     
     2341 agacccgccg aagaagccac ctctttggag ggtgcgctct ctggcacgcg ccactcccac     
     2401 ccatccgtgg gccgccagca ccacgcgggc cccccatcca catcgcggcc accacgtccc     
     2461 tgggacacgc cttgtccccc ggtgtacgcc gagaccaagc acttcctcta ctcctcaggc     
     2521 gacaaggagc agctgcggcc ctccttccta ctcagctctc tgaggcccag cctgactggc     
     2581 gctcggaggc tcgtggagac catctttctg ggttccaggc cctggatgcc agggactccc     
     2641 cgcaggttgc cccgcctgcc ccagcgctac tggcaaatgc ggcccctgtt tctggagctg     
     2701 cttgggaacc acgcgcagtg cccctacggg gtgctcctca agacgcactg cccgctgcga     
     2761 gctgcggtca ccccagcagc cggtgtctgt gcccgggaga agccccaggg ctctgtggcg     
     2821 gcccccgagg aggaggacac agacccccgt cgcctggtgc agctgctccg ccagcacagc     
     2881 agcccctggc aggtgtacgg cttcgtgcgg gcctgcctgc gccggctggt gcccccaggc     
     2941 ctctggggct ccaggcacaa cgaacgccgc ttcctcagga acaccaagaa gttcatctcc     
     3001 ctggggaagc atgccaagct ctcgctgcag gagctgacgt ggaagatgag cgtgcgggac     
     3061 tgcgcttggc tgcgcaggag cccaggggtt ggctgtgttc cggccgcaga gcaccgtctg     
     3121 cgtgaggaga tcctggccaa gttcctgcac tggctgatga gtgtgtacgt cgtcgagctg     
     3181 ctcaggtctt tcttttatgt cacggagacc acgtttcaaa agaacaggct ctttttctac     
     3241 cggaagagtg tctggagcaa gttgcaaagc attggaatca gacagcactt gaagagggtg     
     3301 cagctgcggg agctgtcgga agcagaggtc aggcagcatc gggaagccag gcccgccctg     
     3361 ctgacgtcca gactccgctt catccccaag cctgacgggc tgcggccgat tgtgaacatg     
     3421 gactacgtcg tgggagccag aacgttccgc agagaaaaga gggccgagcg tctcacctcg     
     3481 agggtgaagg cactgttcag cgtgctcaac tacgagcggg cgcggcgccc cggcctcctg     
     3541 ggcgcctctg tgctgggcct ggacgatatc cacagggcct ggcgcacctt cgtgctgcgt     
     3601 gtgcgggccc aggacccgcc gcctgagctg tactttgtca aggtggatgt gacgggcgcg     
     3661 tacgacacca tcccccagga caggctcacg gaggtcatcg ccagcatcat caaaccccag     
     3721 aacacgtact gcgtgcgtcg gtatgccgtg gtccagaagg ccgcccatgg gcacgtccgc     
     3781 aaggccttca agagccacgt ctctaccttg acagacctcc agccgtacat gcgacagttc     
     3841 gtggctcacc tgcaggagac cagcccgctg agggatgccg tcgtcatcga gcagagctcc     
     3901 tccctgaatg aggccagcag tggcctcttc gacgtcttcc tacgcttcat gtgccaccac     
     3961 gccgtgcgca tcaggggcaa gtcctacgtc cagtgccagg ggatcccgca gggctccatc     
     4021 ctctccacgc tgctctgcag cctgtgctac ggcgacatgg agaacaagct gtttgcgggg     
     4081 attcggcggg acgggctgct cctgcgtttg gtggatgatt tcttgttggt gacacctcac     
     4141 ctcacccacg cgaaaacctt cctcaggacc ctggtccgag gtgtccctga gtatggctgc     
     4201 gtggtgaact tgcggaagac agtggtgaac ttccctgtag aagacgaggc cctgggtggc     
     4261 acggcttttg ttcagatgcc ggcccacggc ctattcccct ggtgcggcct gctgctggat     
     4321 acccggaccc tggaggtgca gagcgactac tccagctatg cccggacctc catcagagcc     
     4381 agtctcacct tcaaccgcgg cttcaaggct gggaggaaca tgcgtcgcaa actctttggg     
     4441 gtcttgcggc tgaagtgtca cagcctgttt ctggatttgc aggtgaacag cctccagacg     
     4501 gtgtgcacca acatctacaa gatcctcctg ctgcaggcgt acaggtttca cgcatgtgtg     
     4561 ctgcagctcc catttcatca gcaagtttgg aagaacccca catttttcct gcgcgtcatc     
     4621 tctgacacgg cctccctctg ctactccatc ctgaaagcca agaacgcagg gatgtcgctg     
     4681 ggggccaagg gcgccgccgg ccctctgccc tccgaggccg tgcagtggct gtgccaccaa     
     4741 gcattcctgc tcaagctgac tcgacaccgt gtcacctacg tgccactcct ggggtcactc     
     4801 aggacagccc agacgcagct gagtcggaag ctcccgggga cgacgctgac tgccctggag     
     4861 gccgcagcca acccggcact gccctcagac ttcaagacca tcctggactg atggccaccc     
     4921 gcccacagcc aggccgagag cagacaccag cagccctgtc acgccgggct ctacgtccca     
     4981 gggagggagg ggcggcccac acccaggccc gcaccgctgg gagtctgagg cctgagtgag     
     5041 tgtttggccg aggcctgcat gtccggctga aggctgagtg tccggctgag gcctgagcga     
     5101 gtgtccagcc aagggctgag tgtccagcac acctgccgtc ttcacttccc cacaggctgg     
     5161 cgctcggctc caccctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc     
     5221 gtttaaaccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc     
     5281 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa     
     5341 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg     
     5401 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg     
     5461 gctctatggc ttctgaggcg gaaagaacca gcagatcgat ctgcttatgg gtattatggg     
     5521 tctgcattaa tgaatcggcc aacggatccc ggtgtgaaat accgcacaga tgcgtaagga     
     5581 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg     
     5641 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat     
     5701 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta     
     5761 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa     
     5821 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc     
     5881 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt     
     5941 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca     
     6001 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg     
     6061 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat     
     6121 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta     
     6181 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct     
     6241 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac     
     6301 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa     
     6361 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa     
     6421 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt     
     6481 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca     
     6541 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca     
     6601 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc     
     6661 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa     
     6721 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc     
     6781 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca     
     6841 acgttgttga aaaaggatct tcacctagat ccttttcacg tagaaagcca gtccgcagaa     
     6901 acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag     
     6961 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt     
     7021 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa     
     7081 gccctgcaaa gtaaactgga tggctttctc gccgccaagg atctgatggc gcaggggatc     
     7141 aagctctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca     
     7201 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac     
     7261 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt     
     7321 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc     
     7381 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg     
     7441 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc     
     7501 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc     
     7561 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat     
     7621 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc     
     7681 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca     
     7741 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga     
     7801 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat     
     7861 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc     
     7921 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaattttgtt     
     7981 aaaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aacatccctt     
     8041 ataaatcaaa agaatagacc gcgatagggt tgagtgttgt tccagtttgg aacaagagtc     
     8101 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg     
     8161 gcccactacg tgaaccatca cccaaatcaa gttttttgcg gtcgaggtgc cgtaaagctc     
     8221 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg     
     8281 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag     
     8341 cggtcacgct gcgcgtaacc accacacccg cgcgcttaat gcgccgctac agggcgcgtc     
     8401 cattcgccat tcaggatcga attaattctt aattaacatc atcaataata tacctt         
//