pAdeno-hTERT Antisense

                            LOCUS       dna                      8459 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    942..947
                     /dbxref="REBASE:NdeI"
     Regulatory_Seq  1279..1318
                     /gene="tetO reg"
     misc_binding    1409..1414
                     /dbxref="REBASE:HindIII"
     misc_binding    1450..1455
                     /dbxref="REBASE:EcoRI"
     promoter        1456..5170
                     /gene="hTERT"
     ORF             4144..4869
                     /sequence="ORF_1 rf(1)"
     misc_binding    5183..5188
                     /dbxref="REBASE:EcoRV"
     misc_binding    5199..5206
                     /dbxref="REBASE:NotI"
     misc_binding    5212..5217
                     /dbxref="REBASE:XbaI"
     misc_binding    5498..5503
                     /dbxref="REBASE:ClaI"
     selectable_marker661..6441
                     /gene="Kan/Neo"
     Rep_Origin      5771..6390
                     /gene="pBR322 origin"
     Promoter        7043..7092
                     /gene="NEOKAN prom"
     ORF             7181..7975
                     /sequence="ORF_2 rf(2)"
     Marker          7184..7972
                     /gene="NTP_II marker"
     Rep_Origin      8080..8385
                     /gene="f1 origin"
BASE COUNT    1748 a   2434 c   2491 g   1786 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtaa gctcagatct ggatctcccg     
      481 atcccctatg gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc     
      541 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa     
      601 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc     
      661 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat     
      721 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac     
      781 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa     
      841 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt     
      901 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc     
      961 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat     
     1021 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc     
     1081 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc     
     1141 tccaccccat tgacgtcaat gggagtttgt tttggaacca aaatcaacgg gactttccaa     
     1201 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg     
     1261 tctatataag cagagctctc cctatcagtg atagagatct ccctatcagt gatagagatc     
     1321 gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg     
     1381 acctccatag aagctagcgt ttaaacttaa gcttggtacc gagctcggat ccactagtcc     
     1441 agtgtggtgg aattcccacc tcggctcgcg gtcggacacc ccttcacttc tgccgtccac     
     1501 acgacctgtg agtcgggaac cgacctgtga gcgagtccgg agtcggcctg tgagtcggaa     
     1561 gtcggcctgt acgtccggag ccggtttgtg agtgagtccg gagtctgagg gtcgccacgc     
     1621 ccggacccac acccggcggg gagggaggga ccctgcatct cgggccgcac tgtcccgacg     
     1681 accacagacg agagccggac cgacacccgc ccaccggtag tcaggtccta ccagaacttc     
     1741 agactcccgt cacggcccaa ccgacgccgg aggtcccgtc agtcgcagca ggggccctcg     
     1801 aaggctgagt cgacgcagac ccgacaggac tcactggggt cctcaccgtg catccactgt     
     1861 gccacagctc agtcgaactc gtccttacga accaccgtgt cggtgacgtg ccggagcctc     
     1921 ccgtctcccg gccgccgcgg gaaccggggg tcgctgtagg gacgcaagaa ccgaaagtcc     
     1981 tacctcatcg tctccctccg gcacagtctc tactgcgcgt cctttttaca ccccaagaag     
     2041 gtttgaacga ctactttacc ctcgacgtcg tgtgtacgca ctttggacat gcggacgtcg     
     2101 tcctcctaga acatctacaa ccacgtgtgg cagacctccg acaagtggac gtttaggtct     
     2161 ttgtccgaca ctgtgaagtc ggcgttctgg ggtttctcaa acgctgcgta caaggagggt     
     2221 cggaacttcg gcgccaactt ccactctgac cgagactacc tccaggcccg tatcgacctc     
     2281 atcagcgaga cgtggaggtc ccaggcccat aggtcgtcgt ccggcgtggt ccccttatcc     
     2341 ggcacccggc cgtagacttg ttttcggcac ggtgggtccc ggagcagaag atgtcccttc     
     2401 aagtggtgac agaaggcgtt caagtggtgc gtcggtatga gtccctgtgg agcctggtcc     
     2461 caggactcct tccaaaagcg cacccactcc actccacagt ggttgttctt tagtaggtgg     
     2521 tttgcgtcct cgtcgggcag ggcggcttag gggcgtttgt cgaacaagag gtacagcggc     
     2581 atcgtgtccg acgtctcgtc gcacctctcc tacctcggga cgccctaggg gaccgtgacc     
     2641 tgcatcctga acggggacta cgcgtgccgc accaccgtgt acttcgcatc cttctgcagc     
     2701 ttctccggtg acgaccggag taagtccctc ctcgagacga gctactgctg ccgtagggag     
     2761 tcgcccgacc agaggacgtc cactcggtgc ttgacagcgt acatgccgac ctccagacag     
     2821 ttccatctct gcaccgagaa cttccggaac gcctgcacgg gtacccgccg gaagacctgg     
     2881 tgccgtatgg ctgcgtgcgt catgcacaag accccaaact actacgaccg ctactggagg     
     2941 cactcggaca ggacccccta ccacagcatg cgcgggcagt gtaggtggaa ctgtttcatg     
     3001 tcgagtccgc cgcccaggac ccgggcgtgt gcgtcgtgct tccacgcggt ccgggacacc     
     3061 tatagcaggt ccgggtcgtg tctccgcggg tcctccggcc ccgcggcgcg ggcgagcatc     
     3121 aactcgtgcg acttgtcacg gaagtgggag ctccactctg cgagccggga gaaaagagac     
     3181 gccttgcaag accgagggtg ctgcatcagg tacaagtgtt agccggcgtc gggcagtccg     
     3241 aacccctact tcgcctcaga cctgcagtcg tcccgcccgg accgaagggc tacgacggac     
     3301 tggagacgaa ggctgtcgag ggcgtcgacg tgggagaagt tcacgacaga ctaaggttac     
     3361 gaaacgttga acgaggtctg tgagaaggcc atctttttct cggacaagaa aactttgcac     
     3421 cagaggcact gtattttctt tctggactcg tcgagctgct gcatgtgtga gtagtcggtc     
     3481 acgtccttga accggtccta gaggagtgcg tctgccacga gacgccggcc ttgtgtcggt     
     3541 tggggacccg aggacgcgtc ggttcgcgtc agggcgtgcg agtagaaggt gcagtcgagg     
     3601 acgtcgctct cgaaccgtac gaaggggtcc ctctacttga agaaccacaa ggactccttc     
     3661 gccgcaagca acacggacct cggggtctcc ggacccccgt ggtcggccgc gtccgtccgg     
     3721 gcgtgcttcg gcatgtggac ggtccccgac gacacgaccg cctcgtcgac gtggtccgct     
     3781 gcccccagac acaggaggag gagcccccgg cggtgtctcg ggaccccgaa gagggcccgt     
     3841 gtctgtggcc gacgacccca ctggcgtcga gcgtcgcccg tcacgcagaa ctcctcgtgg     
     3901 ggcatccccg tgacgcgcac caagggttcg tcgaggtctt tgtccccggc gtaaacggtc     
     3961 atcgcgaccc cgtccgcccc gttggacgcc cctcagggac cgtaggtccc ggaccttggg     
     4021 tctttctacc agaggtgctc ggaggctcgc ggtcagtccg acccggagtc tctcgactca     
     4081 tccttcctcc cggcgtcgac gaggaacagc ggactcctca tctccttcac gaaccagagc     
     4141 cgcatgtggc cccctgttcc gcacagggtc cctgcaccac cggcgctaca cctacccccc     
     4201 gggcgcacca cgaccgccgg gtgcctaccc accctcaccg cgcacggtct ctcgcgtggg     
     4261 aggtttctcc accgaagaag ccgcccagac cgtccactgt ggtgtgtctt tggtgccagt     
     4321 gagccaggtg cgcaggacgg gcccacccgg gtcctgggga cgggttgccc gcaggcgagg     
     4381 ccgagtcccc gtcgcggtgc ggacccggag aacccgttgc cgtctgaagc cgaccgtgac     
     4441 gggggcgcgg aggagcgtgg gccccgaccg tccgggtccc cctggggccg gagggactgc     
     4501 gataccaagg tccgggcaag cgtagggtct gcggaagccc caggtgatcg cacaccgccc     
     4561 ccggcccgga ctcaccgtcg cggctcgacc atgtcgccgc cgggcgtgtg gaccatccgc     
     4621 gtcgaccctc ggtggtcgtg tttctcgcgc gtcgcacggt cgtccacttg gtcgtgcagc     
     4681 agcgggtgcg ccgcgtcgtc gtcgggggtg cggggcgagg gggcgtcacg cagccagtgg     
     4741 cacaacccgt ccatcgacgc gtgcgaccac cacttccgga gcccccccgg gggcgcccgg     
     4801 ggcaggtcgt cgcgcttcgg cttccggtcg tgcaagaagc gcggcgcgag cgtgtcggag     
     4861 acgtcgtgag cccggtggtc gaggaagtcc gtcctgtgga ccgccttcct cccccgccgc     
     4921 cccccgccgg cacgcagggt cccgtgcgtg tggtccgtga cccggtggtc gcgcgccttt     
     4981 cggcggccca ggggcgcgac gtggtcggcg gtcgggaccc cggggtccgc ggcgtgcttg     
     5041 caccggtcgc cgtcgtggag cgccatcacc gacgcgtcgt ccctcgcgtg ccgagccgtc     
     5101 gcccctcgcg cgccgtagcg cccccaccgg ccccggtccc gaagggtgca cgcgtcgtcc     
     5161 tgcgtcgcga cggacccctg cagatatcca gcacagtggc ggccgctcga gtctagaggg     
     5221 cccgtttaaa ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt     
     5281 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat     
     5341 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg     
     5401 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg     
     5461 tgggctctat ggcttctgag gcggaaagaa ccagcagatc gatctgctta tgggtattat     
     5521 gggtctgcat taatgaatcg gccaacggat cccggtgtga aataccgcac agatgcgtaa     
     5581 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg     
     5641 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag     
     5701 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc     
     5761 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca     
     5821 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt     
     5881 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc     
     5941 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc     
     6001 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc     
     6061 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact     
     6121 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg     
     6181 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta     
     6241 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca     
     6301 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa     
     6361 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg     
     6421 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc     
     6481 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg     
     6541 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat     
     6601 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg     
     6661 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa     
     6721 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca     
     6781 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc     
     6841 gcaacgttgt tgaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca     
     6901 gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc     
     6961 aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc     
     7021 ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg     
     7081 gaagccctgc aaagtaaact ggatggcttt ctcgccgcca aggatctgat ggcgcagggg     
     7141 atcaagctct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt     
     7201 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca     
     7261 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct     
     7321 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct     
     7381 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc     
     7441 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct     
     7501 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga     
     7561 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg     
     7621 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc     
     7681 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac     
     7741 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat     
     7801 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga     
     7861 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc     
     7921 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaatttt     
     7981 gttaaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaacatcc     
     8041 cttataaatc aaaagaatag accgcgatag ggttgagtgt tgttccagtt tggaacaaga     
     8101 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg     
     8161 atggcccact acgtgaacca tcacccaaat caagtttttt gcggtcgagg tgccgtaaag     
     8221 ctctaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga     
     8281 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg     
     8341 tagcggtcac gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc tacagggcgc     
     8401 gtccattcgc cattcaggat cgaattaatt cttaattaac atcatcaata atatacctt      
//