pLenti-3UTR-Dual-Luc
| Vector Name | pLenti-3UTR-Dual-Luc |
| VectorType | Lentiviral Plasmid |
| Antibiotic Information | Bacterial: Kanamycin |
| Sequencing Primers | Luciferase forward sequencing primer: 5'-GCAAGTTGGACGCCCGCAAGATC-3' SV40 reverse sequencing primer: 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | CMV driving expression of firefly luciferase (P. pyralis), followed by a MCS for cloning in a 3'UTR sequence. A second Renilla luciferase (R. reniformis) is driven by the SV40 promoter to allow for normalization. |
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***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
TTTTGGATTGAAGCCAATATGATAATGAGGGGGTGGAGTTTGTGACGTGGCGCGGGGCGTGGGAACGGGGCGGGTGACGTAGTAGTGTGGCGGAAGTGTGATGTTGCAAGTGTGGCGGAACACATGTAAGCGACGGATGTGGCAAAAGTGACGTTTTTGGTGTGCGCCGGTGTACACAGGAAGTGACAATTTTCGCGCGGTTTTAGGCGGATGTTGTAGTAAATTTGGGCGTAACCGAGTAAGATTTGGCCATTTTCGCGGGAAAACTGAATAAGAGGAAGTGAAATCTGAATAATTTTGTGTTACTCATAGCGCGTAATACGGCAGACCTCAGCGCTAGATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTTAACTATAACGGTCCTAAGGTAGCGAAAATGTAGTCTTATGCAATACTCTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAACATGCCTTACAAGGAGAGAAAAAGCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGATCGTGCCTTATTAGGAAGGCAACAGACGGGTCTGACATGGATTGGACGAACCACTGAATTGCCGCATTGCAGAGATATTGTATTTAAGTGCCTAGCTCGATACATAAACGGGTCTCTCTGGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAAAGCTTGCCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGACTCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCAGTGGCGCCCGAACAGGGACTTGAAAGCGAAAGGGAAACCAGAGGAGCTCTCTCGACGCAGGACTCGGCTTGCTGAAGCGCGCACGGCAAGAGGCGAGGGGCGGCGACTGGTGAGTACGCCAAAAATTTTGACTAGCGGAGGCTAGAAGGAGAGAGATGGGTGCGAGAGCGTCAGTATTAAGCGGGGGAGAATTAGATCGCGATGGGAAAAAATTCGGTTAAGGCCAGGGGGAAAGAAAAAATATAAATTAAAACATATAGTATGGGCAAGCAGGGAGCTAGAACGATTCGCAGTTAATCCTGGCCTGTTAGAAACATCAGAAGGCTGTAGACAAATACTGGGACAGCTACAACCATCCCTTCAGACAGGATCAGAAGAACTTAGATCATTATATAATACAGTAGCAACCCTCTATTGTGTGCATCAAAGGATAGAGATAAAAGACACCAAGGAAGCTTTAGACAAGATAGAGGAAGAGCAAAACAAAAGTAAGACCACCGCACAGCAAGCCCGCTGATCTTCAGACCTGGAGGAGGAGATATGAGGGACATTGGAGAAGTGAATTATATAAATATAAAGTAGTAAAAATTGAACCATTAGGAGTAGCACCCACCAAGGCAAAGAGAAGAGTGGTGCAGAGAGAAAAAAGAGCAGTGGGAATAGGAGCTTTGTTCCTTGGGTTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACGCTGACGGTACAGGCCAGACAATTATTGTCTGGTATAGTGCAGCAGCAGAACAATTTGCTGAGGGCTATTGAGGCGCAACAGCATCTGTTGCAACTCACAGTCTGGGGCATCAAGCAGCTCCAGGCAAGAATCCTGGCTGTGGAAAGATACCTAAAGGATCAACAGCTCCTGGGGATTTGGGGTTGCTCTGGAAAACTCATTTGCACCACTGCTGTGCCTTGGAATGCTAGTTGGAGTAATAAATCTCTGGAACAGATTTGGAATCACACGACCTGGATGGAGTGGGACAGAGAAATTAACAATTACACAAGCTTAATACACTCCTTAATTGAAGAATCGCAAAACCAGCAAGAAAAGAATGAACAAGAATTATTGGAATTAGATAAATGGGCAAGTTTGTGGAATTGGTTTAACATAACAAATTGGCTGTGGTATATAAAATTATTCATAATGATAGTAGGAGGCTTGGTAGGTTTAAGAATAGTTTTTGCTGTACTTTCTATAGTGAATAGAGTTAGGCAGGGATATTCACCATTATCGTTTCAGACCCACCTCCCAACCCCGAGGGGACCCGACAGGCCCGAAGGAATAGAAGAAGAAGGTGGAGAGAGAGACAGAGACAGATCCATTCGATTAGTGAACGGATCTCGACGGTATCGAAAGCTTGGGATTCGAATTTAAAAGAAAAGGGGGGATTGGGGGGTACAGTGCAGGGGAAAGAATAGTAGACATAATAGCAACAGACATACAAACTAAAGAACTACAAAAACAAATTACAAAAATTCAAAATTTTCGGGTTTTTCGAACCTAGGGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTTTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCCATAGAAGAACCGAGTTTAAACTCCCTATCAGTGATAGAGATCTCCCTATCAGTGATAGAGAGCTAGAGCTAGCAGCCACCATGGAAGATGCCAAAAACATTAAGAAGGGCCCAGCGCCATTCTACCCACTCGAAGACGGGACCGCCGGCGAGCAGCTGCACAAAGCCATGAAGCGCTACGCCCTGGTGCCCGGCACCATCGCCTTTACCGACGCACATATCGAGGTGGACATTACCTACGCCGAGTACTTCGAGATGAGCGTTCGGCTGGCAGAAGCTATGAAGCGCTATGGGCTGAATACAAACCATCGGATCGTGGTGTGCAGCGAGAATAGCTTGCAGTTCTTCATGCCCGTGTTGGGTGCCCTGTTCATCGGTGTGGCTGTGGCCCCAGCTAACGACATCTACAACGAGCGCGAGCTGCTGAACAGCATGGGCATCAGCCAGCCCACCGTCGTATTCGTGAGCAAGAAAGGGCTGCAAAAGATCCTCAACGTGCAAAAGAAGCTACCGATCATACAAAAGATCATCATCATGGATAGCAAGACCGACTACCAGGGCTTCCAAAGCATGTACACCTTCGTGACTTCCCATTTGCCACCCGGCTTCAACGAGTACGACTTCGTGCCCGAGAGCTTCGACCGGGACAAAACCATCGCCCTGATCATGAACAGTAGTGGCAGTACCGGATTGCCCAAGGGCGTAGCCCTACCGCACCGCACCGCTTGTGTCCGATTCAGTCATGCCCGCGACCCCATCTTCGGCAACCAGATCATCCCCGACACCGCTATCCTCAGCGTGGTGCCATTTCACCACGGCTTCGGCATGTTCACCACGCTGGGCTACTTGATCTGCGGCTTTCGGGTCGTGCTCATGTACCGCTTCGAGGAGGAGCTATTCTTGCGCAGCTTGCAAGACTATAAGATTCAATCTGCCCTGCTGGTGCCCACACTATTTAGCTTCTTCGCTAAGAGCACTCTCATCGACAAGTACGACCTAAGCAACTTGCACGAGATCGCCAGCGGCGGGGCGCCGCTCAGCAAGGAGGTAGGTGAGGCCGTGGCCAAACGCTTCCACCTACCAGGCATCCGCCAGGGCTACGGCCTGACAGAAACAACCAGCGCCATTCTGATCACCCCCGAAGGGGACGACAAGCCTGGCGCAGTAGGCAAGGTGGTGCCCTTCTTCGAGGCTAAGGTGGTGGACTTGGACACCGGTAAGACACTGGGTGTGAACCAGCGCGGCGAGCTGTGCGTCCGTGGCCCCATGATCATGAGCGGCTACGTTAACAACCCCGAGGCTACAAACGCTCTCATCGACAAGGACGGCTGGCTGCACAGCGGCGACATCGCCTACTGGGACGAGGACGAGCACTTCTTCATCGTGGACCGGCTGAAGAGCCTGATCAAATACAAGGGCTACCAGGTAGCCCCAGCCGAACTGGAGAGCATCCTGCTGCAACACCCCAACATCTTCGACGCCGGGGTCGCCGGCCTGCCCGACGACGATGCCGGCGAGCTGCCCGCCGCAGTCGTCGTGCTGGAACACGGTAAAACCATGACCGAGAAGGAGATCGTGGACTATGTGGCCAGCCAGGTTACAACCGCCAAGAAGCTGCGCGGTGGTGTTGTGTTCGTGGACGAGGTGCCTAAAGGACTGACCGGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGCAAGATCGCCGTGCAATTGTAAGGTACCAGTGTGGTGGCCTGCAGGTGAATTCACTAGTACCGGTAGGCCTGTCGACGATATCGGGCCCGCGGCCGCTGGATCCTCTAGACTGCAGCTCGAGTACCCATACGACGTCCCAGACTACGCTTGAGTTTAAACACGCGTGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCGATATCGCCACCATGTTGTTGAAAGTTGTGTTTGCTATTGGATGTATCGTAGTGCAGGCTATGGCCTCAAAAGTGTACGATCCGGAGCAGCGGAAGAGGATGATCACGGGGCCCCAATGGTGGGCACGATGCAAGCAGATGAATGTGTTGGACAGTTTCATTAACTACTACGACAGCGAGAAACACGCGGAGAACGCAGTGATATTCCTGCACGGCAATGCAACCAGTAGCTATCTGTGGAGACACGTGGTGCCTCATATTGAGCCGGTCGCTAGATGCATTATTCCCGATCTTATTGGAATGGGGAAATCCGGAAAGAGTGGAAATGGATCATATAGGCTCCTCGATCATTATAAATATCTGACTGCTTGGTTTGAATTGCTCAATCTGCCCAAGAAAATCATCTTTGTAGGACATGATTGGGGCTCCGCCCTTGCTTTTCATTATGCCTATGAACACCAGGATCGGATCAAGGCTATTGTTCACATGGAGAGCGTGGTGGATGTGATTGAATCATGGATGGGTTGGCCGGATATAGAAGAAGAGCTGGCGCTGATTAAATCTGAGGAGGGCGAGAAGATGGTACTCGAAAATAACTTCTTTGTCGAGACGGTACTGCCCAGTAAGATCATGCGCAAACTGGAGCCTGAAGAGTTTGCGGCTTACCTGGAACCCTTCAAGGAGAAGGGAGAGGTGAGGAGACCGACCCTGTCATGGCCTCGGGAAATTCCGCTGGTCAAAGGAGGGAAGCCAGACGTCGTCGCCATTGTCCGGAATTACAACGCTTACCTCCGCGCTAGTGACGACCTGCCTAAACTCTTCATCGAATCAGATCCTGGTTTCTTTAGTAACGCCATCGTCGAGGGCGCCAAGAAGTTTCCAAACACCGAATTTGTTAAAGTCAAAGGACTTCACTTCCTCCAGGAGGATGCGCCCGATGAAATGGGAAAGTATATCAAATCCTTCGTGGAGAGGGTCTTGAAGAATGAGCAGAGGTCCATCGAGGGCAGAGGAAGTCTTCTAACATGCGGTGACGTGGAGGAGAATCCCGGCCCTATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCTCGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTGGACCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGGCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCTGAACGCGTTCCGGAAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCTGTCCGGATGGAAGGGCTAATTCACTCCCAACGAATACAAGATCTGCTTTTTGCTTGTACTGGGTCTCTCTGGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAAAGCTTGCCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGACTCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCAGTAGTAGTTCATGTCATCTTATTATTCAGTATTTATAACTTGCAAAGAAATGAATATCAGAGAGTGAGAGGAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGCATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGGCATTATGGGTATTATGGGTCTGCATTAATGAATCGGCCAACGATCCCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGAAAAAGGATCTTCACCTAGATCCTTTTCACGTAGAAAGCCAGTCCGCAGAAACGGTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATGGCGATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTCGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGCTCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAAGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGAGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAATTTTGTTAAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAACATCCCTTATAAATCAAAAGAATAGACCGCGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCAAATCAAGTTTTTTGCGGTCGAGGTGCCGTAAAGCTCTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCGCGCTTAATGCGCCGCTACAGGGCGCGTCCATTCGCCATTCAGGATCGAATTAATTCTTAATTAACATCATCAATAATATACCTT
FEATURES Location/Qualifierssource 1..10879/organism="synthetic DNA construct"/mol_type="other DNA"LTR 715..895/note="5' LTR"/note="truncated 5' long terminal repeat (LTR) from HIV-1"/note="color: #ffe4c4"misc_feature 942..1067/note="HIV-1 psi"/note="packaging signal of human immunodeficiency virustype 1"/note="color: #ffe4c4"misc_feature 1557..1790/note="HIV-1 RRE"/note="The Rev response element (RRE) of humanimmunodeficiency virus type 1 allows for Rev-dependent mRNAexport from the nucleus to the cytoplasm."/note="color: #ffe4c4"enhancer 2437..2741/note="CMV enhancer"/note="human cytomegalovirus immediate early enhancer"/note="color: #ffffff"promoter 2742..2945/note="CMV promoter"/note="human cytomegalovirus (CMV) immediate earlypromoter"/note="color: #ffffff; direction: RIGHT"protein_bind 3022..3040/gene="tetO"/bound_moiety="tetracycline repressor TetR"/note="tet operator"/note=""/note="color: #31849b"protein_bind 3043..3061/gene="tetO"/bound_moiety="tetracycline repressor TetR"/note="tet operator"/note=""/note="color: #31849b"CDS 4840..4866/codon_start=1/product="HA (human influenza hemagglutinin) epitope tag"/note="HA"/note="color: #cc99b2"/translation="YPYDVPDYA"rep_origin 5051..5186/note="SV40 ori"/note="SV40 origin of replication"/note="color: #ffff00"CDS 6186..6197/codon_start=1/product="Factor Xa recognition and cleavage site"/note="Factor Xa site"/note="color: #cc99b2Cleavage site after base 6197"/translation="IEGR"CDS 6243..6842/codon_start=1/gene="pac from Streptomyces alboniger"/product="puromycin N-acetyltransferase"/note="PuroR"/note="confers resistance to puromycin"/note="color: #ccffcc"/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"misc_feature 6855..7442/note="WPRE"/note="woodchuck posttranscriptional regulatory element"/note="color: #ffffff"CDS complement(7326..7337)/codon_start=1/product="Factor Xa recognition and cleavage site"/note="Factor Xa site"/note="color: #cc99b2Cleavage site after base 7325"/translation="IEGR"misc_feature 7452..7685/note="3'LTR (Delta-U3)"/note="self-inactivating 3' long terminal repeat (LTR) fromHIV-1"/note="color: #ffe4c4"LTR 7505..7685/note="5' LTR"/note="truncated 5' long terminal repeat (LTR) from HIV-1"/note="color: #ffe4c4"polyA_signal 7757..7878/note="SV40 poly(A) signal"/note="SV40 polyadenylation signal"/note="color: #a6acb3"rep_origin complement(8206..8794)/direction=LEFT/note="ori"/note="high-copy-number colE1/pMB1/pBR322/pUC origin ofreplication"/note="color: #ffff00"CDS 9601..10395/codon_start=1/gene="aph(3')-II (or nptII)"/product="aminoglycoside phosphotransferase from Tn5"/note="NeoR/KanR"/note="confers resistance to neomycin, kanamycin, and G418(Geneticin)"/note="color: #ccffcc"/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"ORIGIN1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag3061 agctagagct agcagccacc atggaagatg ccaaaaacat taagaagggc ccagcgccat3121 tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg aagcgctacg3181 ccctggtgcc cggcaccatc gcctttaccg acgcacatat cgaggtggac attacctacg3241 ccgagtactt cgagatgagc gttcggctgg cagaagctat gaagcgctat gggctgaata3301 caaaccatcg gatcgtggtg tgcagcgaga atagcttgca gttcttcatg cccgtgttgg3361 gtgccctgtt catcggtgtg gctgtggccc cagctaacga catctacaac gagcgcgagc3421 tgctgaacag catgggcatc agccagccca ccgtcgtatt cgtgagcaag aaagggctgc3481 aaaagatcct caacgtgcaa aagaagctac cgatcataca aaagatcatc atcatggata3541 gcaagaccga ctaccagggc ttccaaagca tgtacacctt cgtgacttcc catttgccac3601 ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa accatcgccc3661 tgatcatgaa cagtagtggc agtaccggat tgcccaaggg cgtagcccta ccgcaccgca3721 ccgcttgtgt ccgattcagt catgcccgcg accccatctt cggcaaccag atcatccccg3781 acaccgctat cctcagcgtg gtgccatttc accacggctt cggcatgttc accacgctgg3841 gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag gagctattct3901 tgcgcagctt gcaagactat aagattcaat ctgccctgct ggtgcccaca ctatttagct3961 tcttcgctaa gagcactctc atcgacaagt acgacctaag caacttgcac gagatcgcca4021 gcggcggggc gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc ttccacctac4081 caggcatccg ccagggctac ggcctgacag aaacaaccag cgccattctg atcacccccg4141 aaggggacga caagcctggc gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg4201 tggacttgga caccggtaag acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg4261 gccccatgat catgagcggc tacgttaaca accccgaggc tacaaacgct ctcatcgaca4321 aggacggctg gctgcacagc ggcgacatcg cctactggga cgaggacgag cacttcttca4381 tcgtggaccg gctgaagagc ctgatcaaat acaagggcta ccaggtagcc ccagccgaac4441 tggagagcat cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg4501 acgacgatgc cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga4561 ccgagaagga gatcgtggac tatgtggcca gccaggttac aaccgccaag aagctgcgcg4621 gtggtgttgt gttcgtggac gaggtgccta aaggactgac cggcaagttg gacgcccgca4681 agatccgcga gattctcatt aaggccaaga agggcggcaa gatcgccgtg caattgtaag4741 gtaccagtgt ggtggcctgc aggtgaattc actagtaccg gtaggcctgt cgacgatatc4801 gggcccgcgg ccgctggatc ctctagactg cagctcgagt acccatacga cgtcccagac4861 tacgcttgag tttaaacacg cgtggtgtgg aaagtcccca ggctccccag caggcagaag4921 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc4981 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct5041 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg5101 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa5161 gtagtgagga ggcttttttg gaggccgata tcgccaccat gttgttgaaa gttgtgtttg5221 ctattggatg tatcgtagtg caggctatgg cctcaaaagt gtacgatccg gagcagcgga5281 agaggatgat cacggggccc caatggtggg cacgatgcaa gcagatgaat gtgttggaca5341 gtttcattaa ctactacgac agcgagaaac acgcggagaa cgcagtgata ttcctgcacg5401 gcaatgcaac cagtagctat ctgtggagac acgtggtgcc tcatattgag ccggtcgcta5461 gatgcattat tcccgatctt attggaatgg ggaaatccgg aaagagtgga aatggatcat5521 ataggctcct cgatcattat aaatatctga ctgcttggtt tgaattgctc aatctgccca5581 agaaaatcat ctttgtagga catgattggg gctccgccct tgcttttcat tatgcctatg5641 aacaccagga tcggatcaag gctattgttc acatggagag cgtggtggat gtgattgaat5701 catggatggg ttggccggat atagaagaag agctggcgct gattaaatct gaggagggcg5761 agaagatggt actcgaaaat aacttctttg tcgagacggt actgcccagt aagatcatgc5821 gcaaactgga gcctgaagag tttgcggctt acctggaacc cttcaaggag aagggagagg5881 tgaggagacc gaccctgtca tggcctcggg aaattccgct ggtcaaagga gggaagccag5941 acgtcgtcgc cattgtccgg aattacaacg cttacctccg cgctagtgac gacctgccta6001 aactcttcat cgaatcagat cctggtttct ttagtaacgc catcgtcgag ggcgccaaga6061 agtttccaaa caccgaattt gttaaagtca aaggacttca cttcctccag gaggatgcgc6121 ccgatgaaat gggaaagtat atcaaatcct tcgtggagag ggtcttgaag aatgagcaga6181 ggtccatcga gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc6241 ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cctcgggccg6301 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtggacccgg6361 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg6421 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg6481 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg6541 gttcccggct ggccgcgcag caacagatgg aagggctcct ggcgccgcac cggcccaagg6601 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg6661 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc6721 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg6781 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct6841 gaacgcgttc cggaaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc6901 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg6961 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc7021 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg7081 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg7141 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga7201 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct7261 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg7321 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc7381 ctcttccgcg tctcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc7441 gcctgtccgg atggaagggc taattcactc ccaacgaata caagatctgc tttttgcttg7501 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa7561 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct7621 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc7681 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat7741 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag7801 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa7861 actcatcaat gtatcttatc atgtctggca tctatgtcgg gtgcggagaa agaggtaatg7921 aaatggcatt atgggtatta tgggtctgca ttaatgaatc ggccaacgat cccggtgtga7981 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct8041 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc8101 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg8161 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg8221 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg8281 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac8341 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca8401 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt8461 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc8521 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag8581 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac8641 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt8701 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa8761 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg8821 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa8881 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat8941 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc9001 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat9061 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc9121 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc9181 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag9241 ttcgccagtt aatagtttgc gcaacgttgt tgaaaaagga tcttcaccta gatccttttc9301 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct9361 atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca9421 tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg9481 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt ctcgccgcca9541 aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc9601 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc9661 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca9721 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg9781 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg9841 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag9901 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg9961 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc10021 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa10081 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac10141 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat10201 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac10261 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc10321 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt10381 gacgagttct tctgaatttt gttaaaattt ttgttaaatc agctcatttt ttaaccaata10441 ggccgaaatc ggcaacatcc cttataaatc aaaagaatag accgcgatag ggttgagtgt10501 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg10561 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcacccaaat caagtttttt10621 gcggtcgagg tgccgtaaag ctctaaatcg gaaccctaaa gggagccccc gatttagagc10681 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg10741 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgcgcgctt10801 aatgcgccgc tacagggcgc gtccattcgc cattcaggat cgaattaatt cttaattaac10861 atcatcaata atatacctt//