pLenti-CMV-DIO-Blank-SV40-Puro

                            LOCUS       dna                      8436 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    457..462
                     /dbxref="REBASE:HpaI"
     misc_binding    457..462
                     /dbxref="REBASE:HincII"
     Other Gene      715..895
                     /gene="HIV-1_5_LTR other"
     Other Gene      1006..1050
                     /gene="HIV-1_psi_pack other"
     misc_binding    1092..1097
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  1557..1790
                     /gene="RRE reg"
     ORF             1600..2301
                     /sequence="ORF_1 rf(1)"
     misc_binding    2611..2616
                     /dbxref="REBASE:NdeI"
     Promoter        2885..2966
                     /gene="CMV prom"
     Regulatory_Seq  3022..3061
                     /gene="tetO reg"
     misc_binding    3068..3073
                     /dbxref="REBASE:SmaI"
     misc_binding    3068..3073
                     /dbxref="REBASE:XmaI"
     misc_binding    3074..3079
                     /dbxref="REBASE:ClaI"
     misc_binding    3098..3103
                     /dbxref="REBASE:KpnI"
     Other Gene      3218..3251
                     /gene="loxP other"
     misc_binding    3261..3266
                     /dbxref="REBASE:EcoRV"
     Other Gene      3383..3416
                     /gene="loxP other"
     misc_binding    3435..3440
                     /dbxref="REBASE:XbaI"
     misc_binding    3441..3446
                     /dbxref="REBASE:PstI"
     Promoter        3497..3765
                     /gene="SV40 prom"
     Rep_Origin      3664..3741
                     /gene="SV40 origin"
     ORF             3731..4399
                     /sequence="ORF_2 rf(2)"
     misc_binding    3744..3756
                     /dbxref="REBASE:SfiI"
     Marker          3800..4399
                     /gene="puro marker"
     Other Gene      5009..5061
                     /gene="delta_U3 other"
     Other Gene      5062..5242
                     /gene="HIV-1_5_LTR other"
     Rep_Origin      5748..6367
                     /gene="pBR322 origin"
     Promoter        7020..7069
                     /gene="NEOKAN prom"
     misc_binding    7127..7132
                     /dbxref="REBASE:BclI"
     ORF             7158..7952
                     /sequence="ORF_3 rf(3)"
     Marker          7161..7949
                     /gene="NTP_II marker"
     Rep_Origin      8057..8362
                     /gene="f1 origin"
BASE COUNT    2149 a   2071 c   2238 g   1978 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta     
      481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag     
      541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg     
      601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact     
      661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct     
      721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt     
      781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac     
      841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc     
      901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc     
      961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa     
     1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg     
     1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata     
     1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc     
     1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga     
     1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc     
     1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca     
     1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag     
     1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta     
     1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga     
     1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg     
     1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg     
     1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc     
     1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg     
     1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat     
     1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac     
     1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat     
     1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca     
     2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga     
     2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg     
     2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa     
     2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga     
     2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag     
     2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa     
     2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat     
     2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt     
     2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa     
     2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc     
     2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct     
     2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt     
     2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg     
     2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca     
     2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca     
     2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc     
     3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag     
     3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accaatcagc tcgctcacca     
     3121 tataacttcg tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc     
     3181 accatcgacc cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta     
     3241 tacgaagtta tctcgagtca gatatcgcta gccaattgat aacttcgtat aggatacttt     
     3301 atacgaagtt atcattggga ttcttcctat tttgatccaa gcatcaccat cgaccctcta     
     3361 gtccagatct caccatcgac ccataacttc gtatagcata cattatacga agttatatgg     
     3421 tgagcaaggg cgattctaga ctgcagctcg agtacccata cgacgtccca gactacgctt     
     3481 gagtttaaac acgcgtggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa     
     3541 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc     
     3601 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg     
     3661 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt     
     3721 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga     
     3781 ggaggctttt ttggaggcca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga     
     3841 cgacgtccct cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg     
     3901 ccacaccgtg gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct     
     3961 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc     
     4021 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg     
     4081 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag ggctcctggc     
     4141 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca     
     4201 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc     
     4261 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct     
     4321 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac     
     4381 ccgcaagccc ggtgcctgaa cgcgttccgg aaatcaacct ctggattaca aaatttgtga     
     4441 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt     
     4501 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa     
     4561 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt     
     4621 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct     
     4681 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg     
     4741 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc     
     4801 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg     
     4861 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct     
     4921 gctgccggct ctgcggcctc ttccgcgtct cgccttcgcc ctcagacgag tcggatctcc     
     4981 ctttgggccg cctccccgcc tgtccggatg gaagggctaa ttcactccca acgaatacaa     
     5041 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc     
     5101 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa     
     5161 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag     
     5221 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa     
     5281 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt     
     5341 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct     
     5401 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggcatct atgtcgggtg     
     5461 cggagaaaga ggtaatgaaa tggcattatg ggtattatgg gtctgcatta atgaatcggc     
     5521 caacgatccc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc     
     5581 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta     
     5641 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag     
     5701 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg     
     5761 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg     
     5821 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg     
     5881 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga     
     5941 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc     
     6001 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt     
     6061 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact     
     6121 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg     
     6181 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt     
     6241 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt     
     6301 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct     
     6361 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg     
     6421 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt     
     6481 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt     
     6541 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc     
     6601 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg     
     6661 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc     
     6721 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg     
     6781 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttga aaaaggatct     
     6841 tcacctagat ccttttcacg tagaaagcca gtccgcagaa acggtgctga ccccggatga     
     6901 atgtcagcta ctgggctatc tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag     
     6961 cttgcagtgg gcttacatgg cgatagctag actgggcggt tttatggaca gcaagcgaac     
     7021 cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga     
     7081 tggctttctc gccgccaagg atctgatggc gcaggggatc aagctctgat caagagacag     
     7141 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt     
     7201 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg     
     7261 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg     
     7321 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg     
     7381 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg     
     7441 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca     
     7501 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc     
     7561 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc     
     7621 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca     
     7681 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga     
     7741 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg     
     7801 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg     
     7861 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg     
     7921 ccttctatcg ccttcttgac gagttcttct gaattttgtt aaaatttttg ttaaatcagc     
     7981 tcatttttta accaataggc cgaaatcggc aacatccctt ataaatcaaa agaatagacc     
     8041 gcgatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac     
     8101 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca     
     8161 cccaaatcaa gttttttgcg gtcgaggtgc cgtaaagctc taaatcggaa ccctaaaggg     
     8221 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag     
     8281 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc     
     8341 accacacccg cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggatcga     
     8401 attaattctt aattaacatc atcaataata tacctt                               
//