pLenti-CMV-DIO-GFP-SV40-Puro

                            LOCUS       dna                      9234 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    457..462
                     /dbxref="REBASE:HpaI"
     misc_binding    457..462
                     /dbxref="REBASE:HincII"
     Other Gene      715..895
                     /gene="HIV-1_5_LTR other"
     Other Gene      1006..1050
                     /gene="HIV-1_psi_pack other"
     misc_binding    1092..1097
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  1557..1790
                     /gene="RRE reg"
     ORF             1600..2301
                     /sequence="ORF_1 rf(1)"
     misc_binding    2611..2616
                     /dbxref="REBASE:NdeI"
     Promoter        2885..2966
                     /gene="CMV prom"
     Regulatory_Seq  3022..3061
                     /gene="tetO reg"
     misc_binding    3068..3073
                     /dbxref="REBASE:XmaI"
     misc_binding    3068..3073
                     /dbxref="REBASE:SmaI"
     misc_binding    3074..3079
                     /dbxref="REBASE:ClaI"
     misc_binding    3098..3103
                     /dbxref="REBASE:KpnI"
     Other Gene      3218..3251
                     /gene="loxP other"
     ORF             3231..4133
                     /sequence="ORF_3 rf(3)"
     ORF             3282..4040
                     /sequence="ORF_5 rf(5)"
     misc_binding    3451..3456
                     /dbxref="REBASE:ApaI"
     Other Gene      4181..4214
                     /gene="loxP other"
     misc_binding    4233..4238
                     /dbxref="REBASE:XbaI"
     Promoter        4295..4563
                     /gene="SV40 prom"
     Rep_Origin      4462..4539
                     /gene="SV40 origin"
     ORF             4529..5197
                     /sequence="ORF_2 rf(2)"
     misc_binding    4542..4554
                     /dbxref="REBASE:SfiI"
     Marker          4598..5197
                     /gene="puro marker"
     Other Gene      5807..5859
                     /gene="delta_U3 other"
     Other Gene      5860..6040
                     /gene="HIV-1_5_LTR other"
     Rep_Origin      6546..7165
                     /gene="pBR322 origin"
     Promoter        7818..7867
                     /gene="NEOKAN prom"
     misc_binding    7925..7930
                     /dbxref="REBASE:BclI"
     ORF             7956..8750
                     /sequence="ORF_4 rf(3)"
     Marker          7959..8747
                     /gene="NTP_II marker"
     Rep_Origin      8855..9160
                     /gene="f1 origin"
BASE COUNT    2272 a   2302 c   2519 g   2141 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta     
      481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag     
      541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg     
      601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact     
      661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct     
      721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt     
      781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac     
      841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc     
      901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc     
      961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa     
     1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg     
     1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata     
     1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc     
     1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga     
     1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc     
     1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca     
     1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag     
     1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta     
     1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga     
     1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg     
     1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg     
     1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc     
     1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg     
     1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat     
     1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac     
     1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat     
     1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca     
     2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga     
     2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg     
     2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa     
     2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga     
     2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag     
     2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa     
     2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat     
     2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt     
     2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa     
     2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc     
     2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct     
     2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt     
     2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg     
     2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca     
     2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca     
     2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc     
     3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag     
     3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accaatcagc tcgctcacca     
     3121 tataacttcg tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc     
     3181 accatcgacc cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta     
     3241 tacgaagtta tctcgagcaa gtttgtacaa aaaagcaggc ttcagcgaga tccggtggag     
     3301 ccgggtccgg cggtgccgtc cacggcagaa ttggacgact gagcgcggga tctggcgaag     
     3361 gcgatggggg tcttgaaggc gtgctggtac tccacgatgc ccagctcggt gttgctgtgc     
     3421 agctcctcca cgcggcggaa ggcgaacatg gggcccccgt tctgcaggat gctggggtgg     
     3481 atggcgctct tgaagtgcat gtggctgtcc accacgaagc tgtagtagcc gccgtcgcgc     
     3541 aggctgaagg tgcgggcgaa gctgcccacc agcacgttat cgcccatggg gtgcaggtgc     
     3601 tccacggtgg cgttgctgcg gatgatcttg tcggtgaaga tcacgctgtc ctcggggaag     
     3661 ccggtgccca ccaccttgaa gtcgccgatc acgcggccgg cctcgtagcg gtagctgaag     
     3721 ctcacgtgca gcacgccgcc gtcctcgtac ttctcgatgc gggtgttggt gtagccgccg     
     3781 ttgttgatgg cgtgcaggaa ggggttctcg tagccgctgg ggtaggtgcc gaagtggtag     
     3841 aagccgtagc ccatcacgtg gctcagcagg taggggctga aggtcagggc gcctttggtg     
     3901 ctcttcatct tgttggtcat gcggccctgc ttgggggtgc cctctccgcc gcccaccagc     
     3961 tcgaactcca cgccgttcag ggtgccggtg atgcggcact cgatctccat ggcgggcagg     
     4021 ccgctctcgt cgctctccat acccagcttt cttgtacaaa gtgggctagc caattgataa     
     4081 cttcgtatag gatactttat acgaagttat cattgggatt cttcctattt tgatccaagc     
     4141 atcaccatcg accctctagt ccagatctca ccatcgaccc ataacttcgt atagcataca     
     4201 ttatacgaag ttatatggtg agcaagggcg attctagact gcagctcgag tacccatacg     
     4261 acgtcccaga ctacgcttga gtttaaacac gcgtggtgtg gaaagtcccc aggctcccca     
     4321 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc     
     4381 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata     
     4441 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg     
     4501 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag     
     4561 ctattccaga agtagtgagg aggctttttt ggaggccatg accgagtaca agcccacggt     
     4621 gcgcctcgcc acccgcgacg acgtccctcg ggccgtacgc accctcgccg ccgcgttcgc     
     4681 cgactacccc gccacgcgcc acaccgtgga cccggaccgc cacatcgagc gggtcaccga     
     4741 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga     
     4801 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt     
     4861 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca     
     4921 gatggaaggg ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt     
     4981 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt     
     5041 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct     
     5101 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc     
     5161 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaacg cgttccggaa atcaacctct     
     5221 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct     
     5281 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat     
     5341 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt     
     5401 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat     
     5461 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc     
     5521 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga     
     5581 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc     
     5641 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga     
     5701 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcg ccttcgccct     
     5761 cagacgagtc ggatctccct ttgggccgcc tccccgcctg tccggatgga agggctaatt     
     5821 cactcccaac gaatacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag     
     5881 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc     
     5941 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga     
     6001 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt     
     6061 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt     
     6121 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca     
     6181 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc     
     6241 tggcatctat gtcgggtgcg gagaaagagg taatgaaatg gcattatggg tattatgggt     
     6301 ctgcattaat gaatcggcca acgatcccgg tgtgaaatac cgcacagatg cgtaaggaga     
     6361 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt     
     6421 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca     
     6481 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa     
     6541 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat     
     6601 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc     
     6661 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc     
     6721 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt     
     6781 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac     
     6841 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg     
     6901 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca     
     6961 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc     
     7021 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa     
     7081 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa     
     7141 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac     
     7201 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta     
     7261 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt     
     7321 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata     
     7381 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc     
     7441 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac     
     7501 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag     
     7561 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac     
     7621 gttgttgaaa aaggatcttc acctagatcc ttttcacgta gaaagccagt ccgcagaaac     
     7681 ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg     
     7741 caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt     
     7801 tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc     
     7861 cctgcaaagt aaactggatg gctttctcgc cgccaaggat ctgatggcgc aggggatcaa     
     7921 gctctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg     
     7981 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa     
     8041 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg     
     8101 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt     
     8161 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa     
     8221 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc     
     8281 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg     
     8341 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg     
     8401 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg     
     8461 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg     
     8521 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact     
     8581 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg     
     8641 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc     
     8701 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attttgttaa     
     8761 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa catcccttat     
     8821 aaatcaaaag aatagaccgc gatagggttg agtgttgttc cagtttggaa caagagtcca     
     8881 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc     
     8941 ccactacgtg aaccatcacc caaatcaagt tttttgcggt cgaggtgccg taaagctcta     
     9001 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg     
     9061 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg     
     9121 gtcacgctgc gcgtaaccac cacacccgcg cgcttaatgc gccgctacag ggcgcgtcca     
     9181 ttcgccattc aggatcgaat taattcttaa ttaacatcat caataatata cctt           
//