pLenti-CMV-DIO-Blank-SV40-GFP

                            LOCUS       dna                      8601 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    457..462
                     /dbxref="REBASE:HpaI"
     misc_binding    457..462
                     /dbxref="REBASE:HincII"
     Other Gene      715..895
                     /gene="HIV-1_5_LTR other"
     Other Gene      1006..1050
                     /gene="HIV-1_psi_pack other"
     misc_binding    1092..1097
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  1557..1790
                     /gene="RRE reg"
     ORF             1600..2301
                     /sequence="ORF_1 rf(1)"
     misc_binding    2611..2616
                     /dbxref="REBASE:NdeI"
     Promoter        2885..2966
                     /gene="CMV prom"
     Regulatory_Seq  3022..3061
                     /gene="tetO reg"
     misc_binding    3068..3073
                     /dbxref="REBASE:SmaI"
     misc_binding    3068..3073
                     /dbxref="REBASE:XmaI"
     misc_binding    3074..3079
                     /dbxref="REBASE:ClaI"
     misc_binding    3098..3103
                     /dbxref="REBASE:KpnI"
     Other Gene      3218..3251
                     /gene="loxP other"
     misc_binding    3261..3266
                     /dbxref="REBASE:EcoRV"
     Other Gene      3383..3416
                     /gene="loxP other"
     misc_binding    3435..3440
                     /dbxref="REBASE:XbaI"
     Promoter        3497..3765
                     /gene="SV40 prom"
     Rep_Origin      3664..3741
                     /gene="SV40 origin"
     ORF             3731..4564
                     /sequence="ORF_2 rf(2)"
     misc_binding    3744..3756
                     /dbxref="REBASE:SfiI"
     misc_binding    4390..4395
                     /dbxref="REBASE:ApaI"
     Other Gene      5174..5226
                     /gene="delta_U3 other"
     Other Gene      5227..5407
                     /gene="HIV-1_5_LTR other"
     Rep_Origin      5913..6532
                     /gene="pBR322 origin"
     Promoter        7185..7234
                     /gene="NEOKAN prom"
     misc_binding    7292..7297
                     /dbxref="REBASE:BclI"
     ORF             7323..8117
                     /sequence="ORF_3 rf(3)"
     Marker          7326..8114
                     /gene="NTP_II marker"
     Rep_Origin      8222..8527
                     /gene="f1 origin"
BASE COUNT    2218 a   2114 c   2256 g   2013 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta     
      481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag     
      541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg     
      601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact     
      661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct     
      721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt     
      781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac     
      841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc     
      901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc     
      961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa     
     1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg     
     1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata     
     1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc     
     1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga     
     1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc     
     1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca     
     1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag     
     1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta     
     1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga     
     1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg     
     1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg     
     1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc     
     1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg     
     1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat     
     1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac     
     1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat     
     1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca     
     2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga     
     2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg     
     2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa     
     2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga     
     2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag     
     2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa     
     2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat     
     2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt     
     2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa     
     2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc     
     2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct     
     2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt     
     2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg     
     2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca     
     2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca     
     2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc     
     3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag     
     3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accaatcagc tcgctcacca     
     3121 tataacttcg tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc     
     3181 accatcgacc cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta     
     3241 tacgaagtta tctcgagtca gatatcgcta gccaattgat aacttcgtat aggatacttt     
     3301 atacgaagtt atcattggga ttcttcctat tttgatccaa gcatcaccat cgaccctcta     
     3361 gtccagatct caccatcgac ccataacttc gtatagcata cattatacga agttatatgg     
     3421 tgagcaaggg cgattctaga ctgcagctcg agtacccata cgacgtccca gactacgctt     
     3481 gagtttaaac acgcgtggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa     
     3541 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc     
     3601 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg     
     3661 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt     
     3721 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga     
     3781 ggaggctttt ttggaggccg ccaccatgga gagcgacgag agcggcctgc ccgccatgga     
     3841 gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg     
     3901 agagggcacc cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct     
     3961 gaccttcagc ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac     
     4021 ctaccccagc ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa     
     4081 cacccgcatc gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta     
     4141 cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag     
     4201 cgtgatcttc accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat     
     4261 gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta     
     4321 ctacagcttc gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct     
     4381 gcagaacggg ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga     
     4441 gctgggcatc gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg     
     4501 cgctcagtcg tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg     
     4561 ctgaacgcgt tccggaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat     
     4621 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca     
     4681 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc     
     4741 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc     
     4801 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt     
     4861 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg     
     4921 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc     
     4981 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta     
     5041 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg     
     5101 gcctcttccg cgtctcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc     
     5161 ccgcctgtcc ggatggaagg gctaattcac tcccaacgaa tacaagatct gctttttgct     
     5221 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg     
     5281 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt     
     5341 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc     
     5401 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga     
     5461 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat     
     5521 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc     
     5581 aaactcatca atgtatctta tcatgtctgg catctatgtc gggtgcggag aaagaggtaa     
     5641 tgaaatggca ttatgggtat tatgggtctg cattaatgaa tcggccaacg atcccggtgt     
     5701 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg     
     5761 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag     
     5821 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa     
     5881 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc     
     5941 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca     
     6001 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg     
     6061 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct     
     6121 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt     
     6181 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag     
     6241 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc     
     6301 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac     
     6361 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga     
     6421 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc     
     6481 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg     
     6541 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca     
     6601 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt     
     6661 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca     
     6721 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg     
     6781 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca     
     6841 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt     
     6901 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt     
     6961 agttcgccag ttaatagttt gcgcaacgtt gttgaaaaag gatcttcacc tagatccttt     
     7021 tcacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg     
     7081 ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta     
     7141 catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg     
     7201 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttctcgccgc     
     7261 caaggatctg atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc     
     7321 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat     
     7381 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt     
     7441 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac     
     7501 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg     
     7561 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc     
     7621 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa     
     7681 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc     
     7741 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg     
     7801 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg     
     7861 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa     
     7921 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg     
     7981 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct     
     8041 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc     
     8101 ttgacgagtt cttctgaatt ttgttaaaat ttttgttaaa tcagctcatt ttttaaccaa     
     8161 taggccgaaa tcggcaacat cccttataaa tcaaaagaat agaccgcgat agggttgagt     
     8221 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg     
     8281 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt     
     8341 ttgcggtcga ggtgccgtaa agctctaaat cggaacccta aagggagccc ccgatttaga     
     8401 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg     
     8461 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgcgcgc     
     8521 ttaatgcgcc gctacagggc gcgtccattc gccattcagg atcgaattaa ttcttaatta     
     8581 acatcatcaa taatatacct t                                               
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