pLenti-CMV-GFP-2A-Puro

                            
FEATURES             Location/Qualifiers
     source          1..8510
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             668..848
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    895..1020
                     /note="HIV-1 psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1510..1743
                     /note="HIV-1 RRE"
                     /note="The Rev response element (RRE) of human 
                     immunodeficiency virus type 1 allows for Rev-dependent mRNA
                     export from the nucleus to the cytoplasm."
                     /note="color: #ffe4c4"
     enhancer        2390..2694
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2695..2898
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    2975..2993
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="
                     "
                     /note="color: #31849b"
     protein_bind    2996..3014
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="
                     "
                     /note="color: #31849b"
     rep_origin      3308..3443
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             4267..4866
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    4879..5466
                     /note="WPRE"
                     /note="woodchuck posttranscriptional regulatory element"
                     /note="color: #ffffff"
     CDS             complement(5350..5361)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /note="color: #cc99b2
                     Cleavage site after base 5349"
                     /translation="IEGR"
     misc_feature    5476..5709
                     /note="3'LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             5529..5709
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    5781..5902
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(6231..6819)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             7231..8025
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
      301 tgttactcat agcgcgtaat actctcatga gcggatacat atttgaatgt atttagaaaa
      361 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtaactataa
      421 cggtcctaag gtagcgaaaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
      481 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
      541 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
      601 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
      661 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
      721 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
      781 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
      841 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
      901 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
      961 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     1021 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     1081 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     1141 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     1201 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     1261 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     1321 gaagagcaaa acaaaagtaa gaccaccgca cagcaagccc gctgatcttc agacctggag
     1381 gaggagatat gagggacatt ggagaagtga attatataaa tataaagtag taaaaattga
     1441 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     1501 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     1561 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     1621 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     1681 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     1741 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     1801 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     1861 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     1921 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     1981 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     2041 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     2101 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     2161 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     2221 tcgacggtat cgaaagcttg ggattcgaat ttaaaagaaa aggggggatt ggggggtaca
     2281 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaactacaaa
     2341 aacaaattac aaaaattcaa aattttcggg tttttcgaac ctagggttcc gcgttacata
     2401 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     2461 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     2521 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     2581 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     2641 atgggacttt cctacttggc agtacatcta cgtttagtca tcgctattac catggtgatg
     2701 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     2761 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     2821 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     2881 gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
     2941 tgttttgacc tccatagaag aaccgagttt aaactcccta tcagtgatag agatctccct
     3001 atcagtgata gagagctagc cccgggatcg atcaattgag tacttacgta ggtaccccag
     3061 tgtggtggcc tgcaggtgaa ttcactagta ccggtaggcc tgtcgacgat atcgggcccg
     3121 cggccgctgg atcctctaga ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     3181 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     3241 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     3301 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     3361 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     3421 gtgaggaggc ttttttggag gcctgtcgag gccaccatgg agagcgacga gagcggcctg
     3481 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg
     3541 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc
     3601 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac
     3661 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc
     3721 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc
     3781 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc
     3841 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac
     3901 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc
     3961 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac
     4021 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac
     4081 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc
     4141 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc
     4201 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc
     4261 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     4321 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     4381 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     4441 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     4501 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     4561 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     4621 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     4681 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     4741 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     4801 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     4861 gcctgaacgc gttccggaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     4921 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     4981 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     5041 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     5101 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     5161 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     5221 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     5281 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     5341 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     5401 cggcctcttc cgcgtctcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     5461 ccccgcctgt ccggatggaa gggctaattc actcccaacg aatacaagat ctgctttttg
     5521 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     5581 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     5641 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     5701 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     5761 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     5821 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5881 ccaaactcat caatgtatct tatcatgtct ggcatctatg tcgggtgcgg agaaagaggt
     5941 aatgaaatgg cattatgggt attatgggtc tgcattaatg aatcggccaa cggatgtcgg
     6001 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     6061 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6121 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6181 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6241 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6301 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6361 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6421 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6481 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6541 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6601 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6661 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6721 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6781 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6841 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggc catgagatta
     6901 tcaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc
     6961 tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag
     7021 agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg
     7081 acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc
     7141 aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagctct
     7201 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     7261 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     7321 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     7381 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
     7441 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     7501 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     7561 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     7621 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     7681 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     7741 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     7801 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     7861 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     7921 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     7981 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaatttt gttaaaattt
     8041 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     8101 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     8161 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     8221 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     8281 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     8341 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     8401 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg
     8461 ccattcagga tcgaattaat tcttaattaa catcatcaat aatatacctt
//