pLenti-E1A-E1B

                            LOCUS       dna                      9378 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    457..462
                     /dbxref="REBASE:HpaI"
     misc_binding    457..462
                     /dbxref="REBASE:HincII"
     Other Gene      715..895
                     /gene="HIV-1_5_LTR other"
     Other Gene      1006..1050
                     /gene="HIV-1_psi_pack other"
     misc_binding    1092..1097
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  1557..1790
                     /gene="RRE reg"
     ORF             1600..2301
                     /sequence="ORF_1 rf(1)"
     misc_binding    2611..2616
                     /dbxref="REBASE:NdeI"
     Promoter        2885..2966
                     /gene="CMV prom"
     ORF             2901..4388
                     /sequence="ORF_3 rf(3)"
     Regulatory_Seq  3022..3061
                     /gene="tetO reg"
     misc_binding    3432..3437
                     /dbxref="REBASE:ClaI"
     misc_binding    3600..3605
                     /dbxref="REBASE:XbaI"
     misc_binding    3977..3982
                     /dbxref="REBASE:PstI"
     misc_binding    4005..4010
                     /dbxref="REBASE:EcoRI"
     misc_binding    4192..4197
                     /dbxref="REBASE:KpnI"
     misc_binding    4389..4394
                     /dbxref="REBASE:XhoI"
     Promoter        4439..4707
                     /gene="SV40 prom"
     Rep_Origin      4606..4683
                     /gene="SV40 origin"
     ORF             4673..5341
                     /sequence="ORF_2 rf(2)"
     misc_binding    4686..4698
                     /dbxref="REBASE:SfiI"
     Marker          4742..5341
                     /gene="puro marker"
     Other Gene      5951..6003
                     /gene="delta_U3 other"
     Other Gene      6004..6184
                     /gene="HIV-1_5_LTR other"
     Rep_Origin      6690..7309
                     /gene="pBR322 origin"
     Promoter        7962..8011
                     /gene="NEOKAN prom"
     misc_binding    8069..8074
                     /dbxref="REBASE:BclI"
     ORF             8100..8894
                     /sequence="ORF_4 rf(3)"
     Marker          8103..8891
                     /gene="NTP_II marker"
     Rep_Origin      8999..9304
                     /gene="f1 origin"
BASE COUNT    2322 a   2322 c   2560 g   2174 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta     
      481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag     
      541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg     
      601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact     
      661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct     
      721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt     
      781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac     
      841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc     
      901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc     
      961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa     
     1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg     
     1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata     
     1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc     
     1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga     
     1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc     
     1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca     
     1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag     
     1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta     
     1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga     
     1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg     
     1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg     
     1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc     
     1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg     
     1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat     
     1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac     
     1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat     
     1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca     
     2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga     
     2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg     
     2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa     
     2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga     
     2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag     
     2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa     
     2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat     
     2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt     
     2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa     
     2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc     
     2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct     
     2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt     
     2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg     
     2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca     
     2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca     
     2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc     
     3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag     
     3061 agctagcagc caccatgaga catattatct gccacggagg tgttattacc gaagaaatgg     
     3121 ccgccagtct tttggaccag ctgatcgaag aggtactggc tgataatctt ccacctccta     
     3181 gccattttga accacctacc cttcacgaac tgtatgattt agacgtgacg gcccccgaag     
     3241 atcccaacga ggaggcggtt tcgcagattt ttcccgactc tgtaatgttg gcggtgcagg     
     3301 aagggattga cttactcact tttccgccgg cgcccggttc tccggagccg cctcaccttt     
     3361 cccggcagcc cgagcagccg gagcagagag ccttgggtcc ggtttctatg ccaaaccttg     
     3421 taccggaggt gatcgatctt acctgccacg aggctggctt tccacccagt gacgacgagg     
     3481 atgaagaggg tcctgtgtct gaacctgagc ctgagcccga gccagaaccg gagcctgcaa     
     3541 gacctacccg ccgtcctaaa atggcgcctg ctatcctgag acgcccgaca tcacctgtgt     
     3601 ctagagaatg caatagtagt acggatagct gtgactccgg tccttctaac acacctcctg     
     3661 agatacaccc ggtggtcccg ctgtgcccca ttaaaccagt tgccgtgaga gttggtgggc     
     3721 gtcgccaggc tgtggaatgt atcgaggact tgcttaacga gcctgggcaa cctttggact     
     3781 tgagctgtaa acgccccagg ccagagggca gaggaagtct tctaacatgc ggtgacgtgg     
     3841 aggagaatcc cggccctatg gaggcttggg agtgtttgga agatttttct gctgtgcgta     
     3901 acttgctgga acagagctct aacagtacct cttggttctg gaggtttctg tggggctcat     
     3961 cccaggcaaa gttagtctgc agaattaagg aggattacaa gtgggaattc gaagagcttt     
     4021 tgaagtcctg tggtgagctg tttgattctt tgaatctggg tcaccaggcg cttctccaag     
     4081 agaaggtcat caagactttg gatttttcca caccggggcg cgctgcggct gctgttgctt     
     4141 ttttgagttt tataaaggat aaatggagcg aagaaaccca tctgagcggg gggtacctgc     
     4201 tggattttct ggccatgcat ctgtggagag cggttgtgag acacaagaat cgcctgctac     
     4261 tgttgtcttc cgtccgcccg gcgataatac cgacggagga gcagcagcag cagcaggagg     
     4321 aagccaggcg gcggcggcag gagcagagcc catggaaccc gagagccggc ctggaccctc     
     4381 gggaatgact cgagtaccca tacgacgtcc cagactacgc ttgagtttaa acacgcgtgg     
     4441 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag     
     4501 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg     
     4561 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact     
     4621 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag     
     4681 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc     
     4741 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ctcgggccgt     
     4801 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tggacccgga     
     4861 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga     
     4921 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga     
     4981 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg     
     5041 ttcccggctg gccgcgcagc aacagatgga agggctcctg gcgccgcacc ggcccaagga     
     5101 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg     
     5161 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct     
     5221 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc     
     5281 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg     
     5341 aacgcgttcc ggaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct     
     5401 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc     
     5461 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct     
     5521 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga     
     5581 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc     
     5641 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac     
     5701 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt     
     5761 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt     
     5821 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc     
     5881 tcttccgcgt ctcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg     
     5941 cctgtccgga tggaagggct aattcactcc caacgaatac aagatctgct ttttgcttgt     
     6001 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac     
     6061 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg     
     6121 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct     
     6181 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata     
     6241 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc     
     6301 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa     
     6361 ctcatcaatg tatcttatca tgtctggcat ctatgtcggg tgcggagaaa gaggtaatga     
     6421 aatggcatta tgggtattat gggtctgcat taatgaatcg gccaacgatc ccggtgtgaa     
     6481 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc     
     6541 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg     
     6601 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc     
     6661 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc     
     6721 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga     
     6781 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc     
     6841 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat     
     6901 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg     
     6961 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc     
     7021 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga     
     7081 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact     
     7141 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt     
     7201 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag     
     7261 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg     
     7321 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa     
     7381 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata     
     7441 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg     
     7501 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata     
     7561 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg     
     7621 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct     
     7681 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt     
     7741 tcgccagtta atagtttgcg caacgttgtt gaaaaaggat cttcacctag atccttttca     
     7801 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta     
     7861 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat     
     7921 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg     
     7981 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc tcgccgccaa     
     8041 ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca     
     8101 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg     
     8161 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag     
     8221 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc     
     8281 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc     
     8341 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg     
     8401 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc     
     8461 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca     
     8521 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag     
     8581 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg     
     8641 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg     
     8701 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca     
     8761 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc     
     8821 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg     
     8881 acgagttctt ctgaattttg ttaaaatttt tgttaaatca gctcattttt taaccaatag     
     8941 gccgaaatcg gcaacatccc ttataaatca aaagaataga ccgcgatagg gttgagtgtt     
     9001 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga     
     9061 aaaaccgtct atcagggcga tggcccacta cgtgaaccat cacccaaatc aagttttttg     
     9121 cggtcgaggt gccgtaaagc tctaaatcgg aaccctaaag ggagcccccg atttagagct     
     9181 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc     
     9241 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgcgcgctta     
     9301 atgcgccgct acagggcgcg tccattcgcc attcaggatc gaattaattc ttaattaaca     
     9361 tcatcaataa tatacctt                                                   
//