pLenti-EF1a-Cas9-Nickase-SV40-Puro

                            
FEATURES             Location/Qualifiers
     source          1..12549
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             700..880
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    927..1052
                     /note="HIV-1 psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1542..1775
                     /note="HIV-1 RRE"
                     /note="The Rev response element (RRE) of human 
                     immunodeficiency virus type 1 allows for Rev-dependent mRNA
                     export from the nucleus to the cytoplasm."
                     /note="color: #ffe4c4"
     CDS             3742..3762
                     /codon_start=1
                     /locus_tag="
                     "
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /note="
                     "
                     /note="color: #993366"
                     /protein_id="
                     "
                     /translation="PKKKRKV"
     CDS             7990..8016
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     rep_origin      8201..8336
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             8337..8936
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    8949..9536
                     /note="WPRE"
                     /note="woodchuck posttranscriptional regulatory element"
                     /note="color: #ffffff"
     CDS             complement(9420..9431)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /note="color: #cc99b2
                     Cleavage site after base 9419"
                     /translation="IEGR"
     misc_feature    9546..9779
                     /note="3'LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             9599..9779
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    9851..9972
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(10301..10889)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             11301..12095
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 ttcttaatta acatcatcaa taatatacct tattttggat tgaagccaat atgataatga
       61 gggggtggag tttgtgacgt ggcgcggggc gtgggaacgg ggcgggtgac gtagtagtgt
      121 ggcggaagtg tgatgttgca agtgtggcgg aacacatgta agcgacggat gtggcaaaag
      181 tgacgttttt ggtgtgcgcc ggtgtacaca ggaagtgaca attttcgcgc ggttttaggc
      241 ggatgttgta gtaaatttgg gcgtaaccga gtaagatttg gccattttcg cgggaaaact
      301 gaataagagg aagtgaaatc tgaataattt tgtgttactc atagcgcgta atactctcat
      361 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
      421 tccccgaaaa gtgccacctg acgtaactat aacggtccta aggtagcgaa aatgtagtct
      481 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
      541 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
      601 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
      661 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
      721 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
      781 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
      841 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
      901 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
      961 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     1021 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     1081 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     1141 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     1201 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     1261 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     1321 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     1381 cacagcaagc ccgctgatct tcagacctgg aggaggagat atgagggaca ttggagaagt
     1441 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1501 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1561 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1621 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1681 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1741 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1801 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1861 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1921 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1981 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     2041 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     2101 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     2161 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     2221 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgaaagct tgggattcga
     2281 atttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     2341 agcaacagac atacaaacta aagaactaca aaaacaaatt acaaaaattc aaaattttcg
     2401 ggtttttcga acctagggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac
     2461 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag
     2521 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     2581 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     2641 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac
     2701 gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga
     2761 tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc
     2821 ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg
     2881 gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg
     2941 atgacctgct gcgacgcttt ttttctggca agatagtctt gtaactgcgg gccaagatct
     3001 gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg
     3061 cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc
     3121 tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg
     3181 ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg
     3241 ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc
     3301 acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga
     3361 gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt
     3421 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga
     3481 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg
     3541 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt
     3601 gtcgtgagga atttcgactg ctagctggta cctgaattga gtacttacgt aggtacccca
     3661 gtgtggtggc ctgcaggtga attcactaga tgtacaatgg ctagtatgca gaaactgatt
     3721 agtgaagagg acctgatggc tcccaagaag aagcgaaagg tgggcatcca cggcgtgccc
     3781 gctgccgaca aaaagtatag tatcggactg gctattggca ctaacagcgt gggatgggcc
     3841 gtcatcaccg acgagtacaa agtgccaagc aagaagttca aggtcctggg aaacaccgat
     3901 agacacagta tcaagaaaaa tctgattgga gccctgctgt tcgactcagg ggagacagct
     3961 gaagcaacta ggctgaaaag aacagctagg agacggtata ctcgccgaaa gaatcggatc
     4021 tgctacctcc aggagatttt ctccaacgaa atggccaagg tggacgatag tttctttcat
     4081 cgcctggagg aatcattcct ggtcgaggaa gataagaaac acgagaggca tcccatcttt
     4141 ggcaacattg tggacgaggt cgcttatcac gaaaagtacc ctacaatcta tcatctgcgg
     4201 aagaaactgg tggacagcac tgataaggca gacctgcgcc tgatctatct ggccctggct
     4261 cacatgatta agttcagggg gcattttctg atcgagggcg atctgaaccc agacaattcc
     4321 gatgtggaca agctgttcat ccagctggtc cagacataca atcagctgtt tgaggaaaac
     4381 cccattaatg catctggggt ggacgcaaaa gccatcctga gtgccagact gtctaagagt
     4441 aggagactgg agaacctgat cgctcagctg ccaggcgaaa agaaaaacgg cctgtttgga
     4501 aatctgattg cactgtcact gggactgacc cccaacttca agagcaattt tgatctggcc
     4561 gaggacgcta agctccagct gagcaaggac acctacgacg atgacctgga taacctgctg
     4621 gctcagatcg gcgatcagta cgcagacctg ttcctggccg ctaagaatct gtctgacgcc
     4681 atcctgctga gtgatattct gagagtgaac accgagatta caaaagcccc cctgtcagct
     4741 agcatgatca agagatatga cgagcaccat caggatctga ccctgctgaa ggctctggtg
     4801 cggcagcagc tgcctgagaa gtacaaagaa atcttctttg atcagagcaa gaatgggtac
     4861 gccggctata ttgacggcgg agcttcccag gaggagttct acaagtttat caaacctatt
     4921 ctggagaaga tggacggcac tgaggaactg ctggtgaaac tgaatcggga agacctgctg
     4981 cggaagcagc gcaccttcga taacggcagc atccctcacc agattcatct gggagagctg
     5041 cacgcaatcc tgcggcgcca ggaagacttc tacccatttc tgaaggataa ccgggagaag
     5101 atcgaaaaaa ttctgacttt ccgcatcccc tactatgtgg ggcctctggc aagaggcaat
     5161 tcccggtttg cctggatgac ccgcaagtct gaggaaacaa tcactccctg gaacttcgag
     5221 gaagtggtcg ataagggcgc ttccgcacag tctttcattg agaggatgac aaattttgac
     5281 aagaacctgc caaatgaaaa agtgctgccc aagcacagcc tgctgtacga gtatttcacc
     5341 gtctataacg aactgacaaa ggtgaaatac gtcactgagg gcatgagaaa gcctgccttc
     5401 ctgtccggag aacagaagaa agctatcgtg gacctgctgt ttaaaaccaa tcggaaggtg
     5461 acagtcaagc agctgaaaga ggactacttc aagaaaattg aatgtttcga ttctgtggag
     5521 atcagtgggg tcgaagacag gtttaacgcc tctctgggca cctaccacga tctgctgaag
     5581 atcattaagg ataaagactt cctggacaac gaggaaaatg aggacatcct ggaggacatt
     5641 gtgctgaccc tgacactgtt tgaggatcgg gaaatgatcg aggaacgcct gaagacctac
     5701 gcccatctgt tcgatgacaa agtgatgaaa cagctgaagc gaaggagata cactgggtgg
     5761 ggccgactga gcaggaagct gatcaatggc attcgcgaca aacagagtgg aaagacaatc
     5821 ctggactttc tgaagtcaga tggcttcgct aacaggaatt ttatgcagct gattcacgat
     5881 gactctctga ctttcaaaga ggacatccag aaggcacagg tgtccggaca gggggactct
     5941 ctgcacgagc atatcgcaaa cctggccggg agccctgcca tcaagaaagg catcctccag
     6001 accgtgaagg tggtggacga gctggtgaaa gtcatgggaa gacataagcc agaaaacatc
     6061 gtgattgaga tggccaggga gaatcagacc acacagaaag ggcagaagaa ctctcgggag
     6121 cgcatgaaac gcatcgagga aggaattaag gaactgggga gtcagatcct gaaagagcac
     6181 cccgtggaaa acacacagct ccagaatgag aagctgtatc tgtactacct ccagaatggc
     6241 cgcgatatgt acgtggacca ggagctggat attaaccgac tgtcagatta tgacgtggat
     6301 catatcgtcc cacagtcatt cctgaaagat gacagcattg acaataaggt gctgacccgc
     6361 agcgacaaaa accgaggaaa gagtgataat gtcccctcag aggaagtggt caagaaaatg
     6421 aagaactact ggaggcagct gctgaatgcc aaactgatca cccagcgaaa gtttgataac
     6481 ctgacaaaag ctgagagggg gggcctgtcc gaactggaca aagcaggctt catcaagcga
     6541 cagctggtgg agacaaggca gatcacaaag cacgtcgctc agatcctgga cagcaggatg
     6601 aacaccaagt acgatgagaa tgacaaactg atccgggaag tgaaggtcat tacactgaag
     6661 tcaaaactgg tgagcgactt taggaaagat ttccagttct acaaggtcag agagatcaac
     6721 aactaccacc atgctcatga cgcatacctg aacgcagtgg tcgggactgc cctgattaag
     6781 aaatacccta aactggagtc tgagttcgtg tacggcgact ataaggtgta cgatgtcaga
     6841 aaaatgatcg ccaagagcga gcaggaaatt ggcaaagcca ccgctaagta tttcttttac
     6901 tccaacatca tgaatttctt taagactgag atcaccctgg caaatggcga aatccgaaag
     6961 aggccactga ttgagactaa cggagagaca ggggaaatcg tgtgggacaa aggaagagat
     7021 tttgctaccg tgcggaaggt cctgagtatg ccccaagtga atattgtcaa gaaaacagag
     7081 gtgcagactg gagggttcag taaggaatca attctgccta aacgcaacag cgataagctg
     7141 atcgcccgaa agaaagactg ggaccccaag aagtatggcg gattcgactc cccaaccgtg
     7201 gcttactctg tcctggtggt cgcaaaggtg gagaagggaa aaagcaagaa actgaaatcc
     7261 gtcaaggaac tgctggggat cacaattatg gagaggagca gcttcgaaaa gaatcctatc
     7321 gattttctgg aggccaaagg gtataaggaa gtgaagaaag acctgatcat caagctgcca
     7381 aagtactctc tgtttgagct ggaaaacggc agaaagcgga tgctggcaag tgccggcgag
     7441 ctgcaaaaag gaaatgaact ggccctgccc tcaaagtacg tgaacttcct gtatctggct
     7501 agccactacg agaagctgaa aggctcccct gaggataacg aacagaaaca gctgtttgtg
     7561 gagcagcaca agcattatct ggacgagatc attgaacaga ttagcgagtt ctccaaacgc
     7621 gtgatcctgg ctgacgcaaa tctggataag gtcctgtctg catacaacaa acacagggac
     7681 aagccaatca gagagcaggc cgaaaatatc attcatctgt tcactctgac caacctggga
     7741 gcccccgcag ccttcaagta ttttgacact accatcgatc gcaaacgata cacaagcact
     7801 aaggaggtgc tggatgctac cctgatccac cagagcatta ctgggctgta cgagacaagg
     7861 atcgacctgt cccagctggg gggagacaaa cgcccagccg ccaccaagaa agcaggacag
     7921 gcaaagaaga agaagtgagt cgacgatatc gggcccgcgg ccgctggatc ctctagactg
     7981 cagctcgagt acccatacga cgtcccagac tacgcttgag tttaaacacg cgtggtgtgg
     8041 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8101 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     8161 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     8221 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8281 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggccatga
     8341 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccctcgg gccgtacgca
     8401 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtggac ccggaccgcc
     8461 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     8521 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     8581 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     8641 ggctggccgc gcagcaacag atggaagggc tcctggcgcc gcaccggccc aaggagcccg
     8701 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     8761 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     8821 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     8881 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaacgc
     8941 gttccggaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     9001 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     9061 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     9121 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     9181 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     9241 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     9301 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     9361 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     9421 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     9481 cgcgtctcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgt
     9541 ccggatggaa gggctaattc actcccaacg aatacaagat ctgctttttg cctgtactgg
     9601 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     9661 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     9721 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     9781 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     9841 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     9901 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     9961 caatgtatct tatcatgtct ggcatctatg tcgggtgcgg agaaagaggt aatgaaatgg
    10021 cattatgggt attatgggtc tgcattaatg aatcggccaa cggatgtcgg tgtgaaatac
    10081 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
    10141 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    10201 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    10261 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    10321 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    10381 aaagatacca ggcgcttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    10441 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    10501 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    10561 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    10621 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    10681 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    10741 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    10801 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    10861 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    10921 acgctcagtg gaacgaaaac tcacgttaag ggattttggc catgagatta tcaaaaagga
    10981 tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc tgaccccgga
    11041 tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg
    11101 tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg
    11161 aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact
    11221 ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagctct gatcaagaga
    11281 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
    11341 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
    11401 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
    11461 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
    11521 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
    11581 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
    11641 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
    11701 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
    11761 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
    11821 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
    11881 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
    11941 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
    12001 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
    12061 tcgccttcta tcgccttctt gacgagttct tctgaatttt gttaaaattt ttgttaaatc
    12121 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
    12181 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
    12241 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
    12301 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
    12361 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
    12421 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
    12481 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcagga
    12541 tcgaattaa
//