pLenti-EF1a-Cas9-Puro

                            
FEATURES             Location/Qualifiers
     source          1..12144
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        27..131
                     /gene="bla"
                     /note="AmpR promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             132..992
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 132 .. 200 / #ccffcc / signal sequence
                        2: 201 .. 992 / #ccffcc
                     Cleavage site after base 200"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     promoter        2075..2105
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 2075 .. 2080 / #ffffff / -35
                        2: 2081 .. 2098 / #ffffff
                        3: 2099 .. 2105 / #ffffff / -10"
     protein_bind    2113..2129
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     2137..2153
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        2174..2192
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             2448..2628
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    2672..2797
                     /note="HIV-1 psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    3290..3523
                     /note="HIV-1 RRE"
                     /note="The Rev response element (RRE) of human 
                     immunodeficiency virus type 1 allows for Rev-dependent mRNA
                     export from the nucleus to the cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    4018..4135
                     /note="HIV-1 cPPT"
                     /note="central polypurine tract from pol gene of human 
                     immunodeficiency virus type 1"
                     /note="color: #ffe4c4"
     CDS             5336..5356
                     /codon_start=1
                     /locus_tag="
                     "
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /note="
                     "
                     /note="color: #993366"
                     /protein_id="
                     "
                     /translation="PKKKRKV"
     CDS             9596..10195
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    10210..10798
                     /note="WPRE"
                     /note="woodchuck posttranscriptional regulatory element"
                     /note="color: #ffffff"
     CDS             complement(10681..10692)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /note="color: #cc99b2
                     Cleavage site after base 10680"
                     /translation="IEGR"
     misc_feature    10885..11118
                     /note="3'LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             10938..11118
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    11190..11311
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      11330..11465
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     promoter        complement(11498..11516)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(11526..11542)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     rep_origin      11687..12142
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt
     4021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     4081 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttccgat
     4141 cacgagacta gcctcgagag ctagcggctc cggtgcccgt cagtgggcag agcgcacatc
     4201 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
     4261 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     4321 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     4381 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg
     4441 ttatggccct tgcgtgcctt gaattacttc cactggctgc agtacgtgat tcttgatccc
     4501 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg
     4561 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg
     4621 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga
     4681 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac
     4741 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca
     4801 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa
     4861 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg
     4921 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct
     4981 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc
     5041 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac
     5101 cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt
     5161 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt
     5221 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct
     5281 tggttcattc tcaagccttc ttccatttca ggtgtcgtga gcgatcgcca tggctcccaa
     5341 gaagaagcga aaggtgggca tccacggcgt gcccgctgcc gacaaaaagt atagtatcgg
     5401 actggatatt ggcactaaca gcgtgggatg ggccgtcatc accgacgagt acaaagtgcc
     5461 aagcaagaag ttcaaggtcc tgggaaacac cgatagacac agtatcaaga aaaatctgat
     5521 tggagccctg ctgttcgact caggggagac agctgaagca actaggctga aaagaacagc
     5581 taggagacgg tatactcgcc gaaagaatcg gatctgctac ctccaggaga ttttctccaa
     5641 cgaaatggcc aaggtggacg atagtttctt tcatcgcctg gaggaatcat tcctggtcga
     5701 ggaagataag aaacacgaga ggcatcccat ctttggcaac attgtggacg aggtcgctta
     5761 tcacgaaaag taccctacaa tctatcatct gcggaagaaa ctggtggaca gcactgataa
     5821 ggcagacctg cgcctgatct atctggccct ggctcacatg attaagttca gggggcattt
     5881 tctgatcgag ggcgatctga acccagacaa ttccgatgtg gacaagctgt tcatccagct
     5941 ggtccagaca tacaatcagc tgtttgagga aaaccccatt aatgcatctg gggtggacgc
     6001 aaaagccatc ctgagtgcca gactgtctaa gagtaggaga ctggagaacc tgatcgctca
     6061 gctgccaggc gaaaagaaaa acggcctgtt tggaaatctg attgcactgt cactgggact
     6121 gacccccaac ttcaagagca attttgatct ggccgaggac gctaagctcc agctgagcaa
     6181 ggacacctac gacgatgacc tggataacct gctggctcag atcggcgatc agtacgcaga
     6241 cctgttcctg gccgctaaga atctgtctga cgccatcctg ctgagtgata ttctgagagt
     6301 gaacaccgag attacaaaag cccccctgtc agctagcatg atcaagagat atgacgagca
     6361 ccatcaggat ctgaccctgc tgaaggctct ggtgcggcag cagctgcctg agaagtacaa
     6421 agaaatcttc tttgatcaga gcaagaatgg gtacgccggc tatattgacg gcggagcttc
     6481 ccaggaggag ttctacaagt ttatcaaacc tattctggag aagatggacg gcactgagga
     6541 actgctggtg aaactgaatc gggaagacct gctgcggaag cagcgcacct tcgataacgg
     6601 cagcatccct caccagattc atctgggaga gctgcacgca atcctgcggc gccaggaaga
     6661 cttctaccca tttctgaagg ataaccggga gaagatcgaa aaaattctga ctttccgcat
     6721 cccctactat gtggggcctc tggcaagagg caattcccgg tttgcctgga tgacccgcaa
     6781 gtctgaggaa acaatcactc cctggaactt cgaggaagtg gtcgataagg gcgcttccgc
     6841 acagtctttc attgagagga tgacaaattt tgacaagaac ctgccaaatg aaaaagtgct
     6901 gcccaagcac agcctgctgt acgagtattt caccgtctat aacgaactga caaaggtgaa
     6961 atacgtcact gagggcatga gaaagcctgc cttcctgtcc ggagaacaga agaaagctat
     7021 cgtggacctg ctgtttaaaa ccaatcggaa ggtgacagtc aagcagctga aagaggacta
     7081 cttcaagaaa attgaatgtt tcgattctgt ggagatcagt ggggtcgaag acaggtttaa
     7141 cgcctctctg ggcacctacc acgatctgct gaagatcatt aaggataaag acttcctgga
     7201 caacgaggaa aatgaggaca tcctggagga cattgtgctg accctgacac tgtttgagga
     7261 tcgggaaatg atcgaggaac gcctgaagac ctacgcccat ctgttcgatg acaaagtgat
     7321 gaaacagctg aagcgaagga gatacactgg gtggggccga ctgagcagga agctgatcaa
     7381 tggcattcgc gacaaacaga gtggaaagac aatcctggac tttctgaagt cagatggctt
     7441 cgctaacagg aattttatgc agctgattca cgatgactct ctgactttca aagaggacat
     7501 ccagaaggca caggtgtccg gacaggggga ctctctgcac gagcatatcg caaacctggc
     7561 cgggagccct gccatcaaga aaggcatcct ccagaccgtg aaggtggtgg acgagctggt
     7621 gaaagtcatg ggaagacata agccagaaaa catcgtgatt gagatggcca gggagaatca
     7681 gaccacacag aaagggcaga agaactctcg ggagcgcatg aaacgcatcg aggaaggaat
     7741 taaggaactg gggagtcaga tcctgaaaga gcaccccgtg gaaaacacac agctccagaa
     7801 tgagaagctg tatctgtact acctccagaa tggccgcgat atgtacgtgg accaggagct
     7861 ggatattaac cgactgtcag attatgacgt ggatcatatc gtcccacagt cattcctgaa
     7921 agatgacagc attgacaata aggtgctgac ccgcagcgac aaaaaccgag gaaagagtga
     7981 taatgtcccc tcagaggaag tggtcaagaa aatgaagaac tactggaggc agctgctgaa
     8041 tgccaaactg atcacccagc gaaagtttga taacctgaca aaagctgaga gggggggcct
     8101 gtccgaactg gacaaagcag gcttcatcaa gcgacagctg gtggagacaa ggcagatcac
     8161 aaagcacgtc gctcagatcc tggacagcag gatgaacacc aagtacgatg agaatgacaa
     8221 actgatccgg gaagtgaagg tcattacact gaagtcaaaa ctggtgagcg actttaggaa
     8281 agatttccag ttctacaagg tcagagagat caacaactac caccatgctc atgacgcata
     8341 cctgaacgca gtggtcggga ctgccctgat taagaaatac cctaaactgg agtctgagtt
     8401 cgtgtacggc gactataagg tgtacgatgt cagaaaaatg atcgccaaga gcgagcagga
     8461 aattggcaaa gccaccgcta agtatttctt ttactccaac atcatgaatt tctttaagac
     8521 tgagatcacc ctggcaaatg gcgaaatccg aaagaggcca ctgattgaga ctaacggaga
     8581 gacaggggaa atcgtgtggg acaaaggaag agattttgct accgtgcgga aggtcctgag
     8641 tatgccccaa gtgaatattg tcaagaaaac agaggtgcag actggagggt tcagtaagga
     8701 atcaattctg cctaaacgca acagcgataa gctgatcgcc cgaaagaaag actgggaccc
     8761 caagaagtat ggcggattcg actccccaac cgtggcttac tctgtcctgg tggtcgcaaa
     8821 ggtggagaag ggaaaaagca agaaactgaa atccgtcaag gaactgctgg ggatcacaat
     8881 tatggagagg agcagcttcg aaaagaatcc tatcgatttt ctggaggcca aagggtataa
     8941 ggaagtgaag aaagacctga tcatcaagct gccaaagtac tctctgtttg agctggaaaa
     9001 cggcagaaag cggatgctgg caagtgccgg cgagctgcaa aaaggaaatg aactggccct
     9061 gccctcaaag tacgtgaact tcctgtatct ggctagccac tacgagaagc tgaaaggctc
     9121 ccctgaggat aacgaacaga aacagctgtt tgtggagcag cacaagcatt atctggacga
     9181 gatcattgaa cagattagcg agttctccaa acgcgtgatc ctggctgacg caaatctgga
     9241 taaggtcctg tctgcataca acaaacacag ggacaagcca atcagagagc aggccgaaaa
     9301 tatcattcat ctgttcactc tgaccaacct gggagccccc gcagccttca agtattttga
     9361 cactaccatc gatcgcaaac gatacacaag cactaaggag gtgctggatg ctaccctgat
     9421 ccaccagagc attactgggc tgtacgagac aaggatcgac ctgtcccagc tggggggaga
     9481 caaacgccca gccgccacca agaaagcagg acaggcaaag aagaagaaga cgcgtcagtg
     9541 tactaattat gctctcttga aattggctgg agatgttgag agcaacccag gtcccatgac
     9601 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     9661 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     9721 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     9781 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     9841 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     9901 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     9961 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
    10021 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
    10081 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
    10141 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgagttta
    10201 aacgtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
    10261 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
    10321 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
    10381 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
    10441 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
    10501 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
    10561 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
    10621 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
    10681 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
    10741 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
    10801 ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
    10861 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
    10921 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
    10981 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
    11041 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
    11101 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
    11161 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
    11221 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
    11281 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
    11341 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
    11401 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
    11461 aggcctaggc ttttgcgtcg agacgtaccc aattcgccct atagtgagtc gtattacgcg
    11521 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
    11581 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
    11641 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcgacgc gccctgtagc
    11701 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
    11761 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
    11821 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
    11881 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
    11941 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
    12001 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
    12061 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
    12121 aaaatattaa cgtttacaat ttcc
//