pLenti-EF1a-hTERT
| Vector Name | pLenti-EF1a-hTERT |
| VectorType | Lentiviral Plasmid |
| Antibiotic Information | No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
| Sequencing Primers | EF1a sequencing primer 5'--TCA GAC AGT GGT TCA AAG--3' SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | No Additional Information |
Double-click on the features for more information
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggagtggga attggctccg gtgcccgtca
2461 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
2521 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
2581 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
2641 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
2701 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca ctggctgcag
2761 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
2821 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
2881 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
2941 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
3001 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
3061 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
3121 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
3181 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
3241 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
3301 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
3361 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
3421 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
3481 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
3541 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
3601 cttccatttc aggtgtcgtg aggaatttcg actgctagcg atatcacaag tttgtacaaa
3661 aaagttggca tgccgcgcgc tccccgctgc cgagccgtgc gctccctgct gcgcagccac
3721 taccgcgagg tgctgccgct ggccacgttc gtgcggcgcc tggggcccca gggctggcgg
3781 ctggtgcagc gcggggaccc ggcggctttc cgcgcgctgg tggcccagtg cctggtgtgc
3841 gtgccctggg acgcacggcc gccccccgcc gccccctcct tccgccaggt gtcctgcctg
3901 aaggagctgg tggcccgagt gctgcagagg ctgtgcgagc gcggcgcgaa gaacgtgctg
3961 gccttcggct tcgcgctgct ggacggggcc cgcgggggcc cccccgaggc cttcaccacc
4021 agcgtgcgca gctacctgcc caacacggtg accgacgcac tgcgggggag cggggcgtgg
4081 gggctgctgc tgcgccgcgt gggcgacgac gtgctggttc acctgctggc acgctgcgcg
4141 ctctttgtgc tggtggctcc cagctgcgcc taccaggtgt gcgggccgcc gctgtaccag
4201 ctcggcgctg ccactcaggc ccggcccccg ccacacgcta gtggaccccg aaggcgtctg
4261 ggatgcgaac gggcctggaa ccatagcgtc agggaggccg gggtccccct gggcctgcca
4321 gccccgggtg cgaggaggcg cgggggcagt gccagccgaa gtctgccgtt gcccaagagg
4381 cccaggcgtg gcgctgcccc tgagccggag cggacgcccg ttgggcaggg gtcctgggcc
4441 cacccgggca ggacgcgtgg accgagtgac cgtggtttct gtgtggtgtc acctgccaga
4501 cccgccgaag aagccacctc tttggagggt gcgctctctg gcacgcgcca ctcccaccca
4561 tccgtgggcc gccagcacca cgcgggcccc ccatccacat cgcggccacc acgtccctgg
4621 gacacgcctt gtcccccggt gtacgccgag accaagcact tcctctactc ctcaggcgac
4681 aaggagcagc tgcggccctc cttcctactc agctctctga ggcccagcct gactggcgct
4741 cggaggctcg tggagaccat ctttctgggt tccaggccct ggatgccagg gactccccgc
4801 aggttgcccc gcctgcccca gcgctactgg caaatgcggc ccctgtttct ggagctgctt
4861 gggaaccacg cgcagtgccc ctacggggtg ctcctcaaga cgcactgccc gctgcgagct
4921 gcggtcaccc cagcagccgg tgtctgtgcc cgggagaagc cccagggctc tgtggcggcc
4981 cccgaggagg aggacacaga cccccgtcgc ctggtgcagc tgctccgcca gcacagcagc
5041 ccctggcagg tgtacggctt cgtgcgggcc tgcctgcgcc ggctggtgcc cccaggcctc
5101 tggggctcca ggcacaacga acgccgcttc ctcaggaaca ccaagaagtt catctccctg
5161 gggaagcatg ccaagctctc gctgcaggag ctgacgtgga agatgagcgt gcgggactgc
5221 gcttggctgc gcaggagccc aggggttggc tgtgttccgg ccgcagagca ccgtctgcgt
5281 gaggagatcc tggccaagtt cctgcactgg ctgatgagtg tgtacgtcgt cgagctgctc
5341 aggtctttct tttatgtcac ggagaccacg tttcaaaaga acaggctctt tttctaccgg
5401 aagagtgtct ggagcaagtt gcaaagcatt ggaatcagac agcacttgaa gagggtgcag
5461 ctgcgggagc tgtcggaagc agaggtcagg cagcatcggg aagccaggcc cgccctgctg
5521 acgtccagac tccgcttcat ccccaagcct gacgggctgc ggccgattgt gaacatggac
5581 tacgtcgtgg gagccagaac gttccgcaga gaaaagaggg ccgagcgtct cacctcgagg
5641 gtgaaggcac tgttcagcgt gctcaactac gagcgggcgc ggcgccccgg cctcctgggc
5701 gcctctgtgc tgggcctgga cgatatccac agggcctggc gcaccttcgt gctgcgtgtg
5761 cgggcccagg acccgccgcc tgagctgtac tttgtcaagg tggatgtgac gggcgcgtac
5821 gacaccatcc cccaggacag gctcacggag gtcatcgcca gcatcatcaa accccagaac
5881 acgtactgcg tgcgtcggta tgccgtggtc cagaaggccg cccatgggca cgtccgcaag
5941 gccttcaaga gccacgtctc taccttgaca gacctccagc cgtacatgcg acagttcgtg
6001 gctcacctgc aggagaccag cccgctgagg gatgccgtcg tcatcgagca gagctcctcc
6061 ctgaatgagg ccagcagtgg cctcttcgac gtcttcctac gcttcatgtg ccaccacgcc
6121 gtgcgcatca ggggcaagtc ctacgtccag tgccagggga tcccgcaggg ctccatcctc
6181 tccacgctgc tctgcagcct gtgctacggc gacatggaga acaagctgtt tgcggggatt
6241 cggcgggacg ggctgctcct gcgtttggtg gatgatttct tgttggtgac acctcacctc
6301 acccacgcga aaaccttcct caggaccctg gtccgaggtg tccctgagta tggctgcgtg
6361 gtgaacttgc ggaagacagt ggtgaacttc cctgtagaag acgaggccct gggtggcacg
6421 gcttttgttc agatgccggc ccacggccta ttcccctggt gcggcctgct gctggatacc
6481 cggaccctgg aggtgcagag cgactactcc agctatgccc ggacctccat cagagccagt
6541 ctcaccttca accgcggctt caaggctggg aggaacatgc gtcgcaaact ctttggggtc
6601 ttgcggctga agtgtcacag cctgtttctg gatttgcagg tgaacagcct ccagacggtg
6661 tgcaccaaca tctacaagat cctcctgctg caggcgtaca ggtttcacgc atgtgtgctg
6721 cagctcccat ttcatcagca agtttggaag aaccccacat ttttcctgcg cgtcatctct
6781 gacacggcct ccctctgcta ctccatcctg aaagccaaga acgcagggat gtcgctgggg
6841 gccaagggcg ccgccggccc tctgccctcc gaggccgtgc agtggctgtg ccaccaagca
6901 ttcctgctca agctgactcg acaccgtgtc acctacgtgc cactcctggg gtcactcagg
6961 acagcccaga cgcagctgag tcggaagctc ccggggacga cgctgactgc cctggaggcc
7021 gcagccaacc cggcactgcc ctcagacttc aagaccatcc tggactgccc aactttcttg
7081 tacaaagtgg tggatatctg aggatcctct agactgcagc tcgagtaccc atacgacgtc
7141 ccagactacg cttgagttta aacacgcgtg gtgtggaaag tccccaggct ccccagcagg
7201 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
7261 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
7321 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
7381 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
7441 ccagaagtag tgaggaggct tttttggagg ccatgaccga gtacaagccc acggtgcgcc
7501 tcgccacccg cgacgacgtc cctcgggccg tacgcaccct cgccgccgcg ttcgccgact
7561 accccgccac gcgccacacc gtggacccgg accgccacat cgagcgggtc accgagctgc
7621 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
7681 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
7741 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
7801 aagggctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
7861 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
7921 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
7981 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
8041 cctggtgcat gacccgcaag cccggtgcct gaacgcgttc cggaaatcaa cctctggatt
8101 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
8161 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
8221 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
8281 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
8341 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
8401 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
8461 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
8521 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
8581 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctcgccttc gccctcagac
8641 gagtcggatc tccctttggg ccgcctcccc gcctgtccgg atggaagggc taattcactc
8701 ccaacgaata caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
8761 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
8821 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
8881 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
8941 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
9001 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
9061 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggca
9121 tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt atgggtatta tgggtctgca
9181 ttaatgaatc ggccaacgat cccggtgtga aataccgcac agatgcgtaa ggagaaaata
9241 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
9301 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
9361 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
9421 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
9481 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
9541 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
9601 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
9661 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
9721 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
9781 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
9841 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
9901 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
9961 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
10021 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
10081 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
10141 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
10201 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
10261 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
10321 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
10381 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
10441 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
10501 tgaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc
10561 tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag
10621 agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg
10681 acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc
10741 aaagtaaact ggatggcttt ctcgccgcca aggatctgat ggcgcagggg atcaagctct
10801 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
10861 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
10921 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
10981 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
11041 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
11101 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
11161 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
11221 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
11281 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
11341 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
11401 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
11461 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
11521 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
11581 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaatttt gttaaaattt
11641 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaacatcc cttataaatc
11701 aaaagaatag accgcgatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
11761 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
11821 acgtgaacca tcacccaaat caagtttttt gcggtcgagg tgccgtaaag ctctaaatcg
11881 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
11941 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
12001 gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc tacagggcgc gtccattcgc
12061 cattcaggat cgaattaatt cttaattaac atcatcaata atatacctt
LOCUS dna 12109 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
ORF 2381..3361
/sequence="ORF_5 rf(4)"
Promoter 2444..3621
/gene="EF1a prom"
ORF 3433..4284
/sequence="ORF_7 rf(5)"
ORF 3670..7101
/sequence="ORF_2 rf(1)"
ORF 4705..5925
/sequence="ORF_6 rf(5)"
misc_binding 7108..7113
/dbxref="REBASE:XbaI"
Promoter 7170..7438
/gene="SV40 prom"
Rep_Origin 7337..7414
/gene="SV40 origin"
ORF 7404..8072
/sequence="ORF_4 rf(3)"
misc_binding 7417..7429
/dbxref="REBASE:SfiI"
Marker 7473..8072
/gene="puro marker"
Other Gene 8682..8734
/gene="delta_U3 other"
Other Gene 8735..8915
/gene="HIV-1_5_LTR other"
Rep_Origin 9421..10040
/gene="pBR322 origin"
Promoter 10693..10742
/gene="NEOKAN prom"
misc_binding 10800..10805
/dbxref="REBASE:BclI"
ORF 10831..11625
/sequence="ORF_3 rf(1)"
Marker 10834..11622
/gene="NTP_II marker"
Rep_Origin 11730..12035
/gene="f1 origin"
BASE COUNT 2644 a 3301 c 3533 g 2631 t 0 others