pLenti-EF1a-hTERT-Hygro
| Vector Name | pLenti-EF1a-hTERT-Hygro |
| VectorType | Lentiviral Plasmid |
| Antibiotic Information | No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
| Sequencing Primers | EF1a forward sequencing primer 5'--TCA GAC AGT GGT TCA AAG--3' SV40 reverse sequencing primer 5'--TAG TCA GCC ATG GGG CGG AGA--3' |
| Additional Information | The gene expression is driven by an EF1a promoter, without any Tag or reporter. |
Double-click on the features for more information
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggagtggga attggctccg gtgcccgtca
2461 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
2521 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
2581 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
2641 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
2701 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca ctggctgcag
2761 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
2821 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
2881 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
2941 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
3001 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
3061 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
3121 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
3181 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
3241 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
3301 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
3361 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
3421 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
3481 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
3541 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
3601 cttccatttc aggtgtcgtg aggaatttcg actgctagcg atatcacaag tttgtacaaa
3661 aaagttggca tgccgcgcgc tccccgctgc cgagccgtgc gctccctgct gcgcagccac
3721 taccgcgagg tgctgccgct ggccacgttc gtgcggcgcc tggggcccca gggctggcgg
3781 ctggtgcagc gcggggaccc ggcggctttc cgcgcgctgg tggcccagtg cctggtgtgc
3841 gtgccctggg acgcacggcc gccccccgcc gccccctcct tccgccaggt gtcctgcctg
3901 aaggagctgg tggcccgagt gctgcagagg ctgtgcgagc gcggcgcgaa gaacgtgctg
3961 gccttcggct tcgcgctgct ggacggggcc cgcgggggcc cccccgaggc cttcaccacc
4021 agcgtgcgca gctacctgcc caacacggtg accgacgcac tgcgggggag cggggcgtgg
4081 gggctgctgc tgcgccgcgt gggcgacgac gtgctggttc acctgctggc acgctgcgcg
4141 ctctttgtgc tggtggctcc cagctgcgcc taccaggtgt gcgggccgcc gctgtaccag
4201 ctcggcgctg ccactcaggc ccggcccccg ccacacgcta gtggaccccg aaggcgtctg
4261 ggatgcgaac gggcctggaa ccatagcgtc agggaggccg gggtccccct gggcctgcca
4321 gccccgggtg cgaggaggcg cgggggcagt gccagccgaa gtctgccgtt gcccaagagg
4381 cccaggcgtg gcgctgcccc tgagccggag cggacgcccg ttgggcaggg gtcctgggcc
4441 cacccgggca ggacgcgtgg accgagtgac cgtggtttct gtgtggtgtc acctgccaga
4501 cccgccgaag aagccacctc tttggagggt gcgctctctg gcacgcgcca ctcccaccca
4561 tccgtgggcc gccagcacca cgcgggcccc ccatccacat cgcggccacc acgtccctgg
4621 gacacgcctt gtcccccggt gtacgccgag accaagcact tcctctactc ctcaggcgac
4681 aaggagcagc tgcggccctc cttcctactc agctctctga ggcccagcct gactggcgct
4741 cggaggctcg tggagaccat ctttctgggt tccaggccct ggatgccagg gactccccgc
4801 aggttgcccc gcctgcccca gcgctactgg caaatgcggc ccctgtttct ggagctgctt
4861 gggaaccacg cgcagtgccc ctacggggtg ctcctcaaga cgcactgccc gctgcgagct
4921 gcggtcaccc cagcagccgg tgtctgtgcc cgggagaagc cccagggctc tgtggcggcc
4981 cccgaggagg aggacacaga cccccgtcgc ctggtgcagc tgctccgcca gcacagcagc
5041 ccctggcagg tgtacggctt cgtgcgggcc tgcctgcgcc ggctggtgcc cccaggcctc
5101 tggggctcca ggcacaacga acgccgcttc ctcaggaaca ccaagaagtt catctccctg
5161 gggaagcatg ccaagctctc gctgcaggag ctgacgtgga agatgagcgt gcgggactgc
5221 gcttggctgc gcaggagccc aggggttggc tgtgttccgg ccgcagagca ccgtctgcgt
5281 gaggagatcc tggccaagtt cctgcactgg ctgatgagtg tgtacgtcgt cgagctgctc
5341 aggtctttct tttatgtcac ggagaccacg tttcaaaaga acaggctctt tttctaccgg
5401 aagagtgtct ggagcaagtt gcaaagcatt ggaatcagac agcacttgaa gagggtgcag
5461 ctgcgggagc tgtcggaagc agaggtcagg cagcatcggg aagccaggcc cgccctgctg
5521 acgtccagac tccgcttcat ccccaagcct gacgggctgc ggccgattgt gaacatggac
5581 tacgtcgtgg gagccagaac gttccgcaga gaaaagaggg ccgagcgtct cacctcgagg
5641 gtgaaggcac tgttcagcgt gctcaactac gagcgggcgc ggcgccccgg cctcctgggc
5701 gcctctgtgc tgggcctgga cgatatccac agggcctggc gcaccttcgt gctgcgtgtg
5761 cgggcccagg acccgccgcc tgagctgtac tttgtcaagg tggatgtgac gggcgcgtac
5821 gacaccatcc cccaggacag gctcacggag gtcatcgcca gcatcatcaa accccagaac
5881 acgtactgcg tgcgtcggta tgccgtggtc cagaaggccg cccatgggca cgtccgcaag
5941 gccttcaaga gccacgtctc taccttgaca gacctccagc cgtacatgcg acagttcgtg
6001 gctcacctgc aggagaccag cccgctgagg gatgccgtcg tcatcgagca gagctcctcc
6061 ctgaatgagg ccagcagtgg cctcttcgac gtcttcctac gcttcatgtg ccaccacgcc
6121 gtgcgcatca ggggcaagtc ctacgtccag tgccagggga tcccgcaggg ctccatcctc
6181 tccacgctgc tctgcagcct gtgctacggc gacatggaga acaagctgtt tgcggggatt
6241 cggcgggacg ggctgctcct gcgtttggtg gatgatttct tgttggtgac acctcacctc
6301 acccacgcga aaaccttcct caggaccctg gtccgaggtg tccctgagta tggctgcgtg
6361 gtgaacttgc ggaagacagt ggtgaacttc cctgtagaag acgaggccct gggtggcacg
6421 gcttttgttc agatgccggc ccacggccta ttcccctggt gcggcctgct gctggatacc
6481 cggaccctgg aggtgcagag cgactactcc agctatgccc ggacctccat cagagccagt
6541 ctcaccttca accgcggctt caaggctggg aggaacatgc gtcgcaaact ctttggggtc
6601 ttgcggctga agtgtcacag cctgtttctg gatttgcagg tgaacagcct ccagacggtg
6661 tgcaccaaca tctacaagat cctcctgctg caggcgtaca ggtttcacgc atgtgtgctg
6721 cagctcccat ttcatcagca agtttggaag aaccccacat ttttcctgcg cgtcatctct
6781 gacacggcct ccctctgcta ctccatcctg aaagccaaga acgcagggat gtcgctgggg
6841 gccaagggcg ccgccggccc tctgccctcc gaggccgtgc agtggctgtg ccaccaagca
6901 ttcctgctca agctgactcg acaccgtgtc acctacgtgc cactcctggg gtcactcagg
6961 acagcccaga cgcagctgag tcggaagctc ccggggacga cgctgactgc cctggaggcc
7021 gcagccaacc cggcactgcc ctcagacttc aagaccatcc tggactgccc aactttcttg
7081 tacaaagtgg tggatatctg aggatcctct agactgcagc tcgagtaccc atacgacgtc
7141 ccagactacg cttgagttta aacacgcgtg gtgtggaaag tccccaggct ccccagcagg
7201 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
7261 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
7321 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
7381 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
7441 ccagaagtag tgaggaggct tttttggagg ccatgaaaaa gcctgaactc accgcgacgt
7501 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
7561 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
7621 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
7681 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt
7741 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
7801 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
7861 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
7921 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
7981 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
8041 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
8101 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
8161 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
8221 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg
8281 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
8341 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
8401 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
8461 accgacgccc cagcactcgt ccgagggcaa aggaatagac gcgttccgga aatcaacctc
8521 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
8581 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
8641 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
8701 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
8761 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
8821 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
8881 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
8941 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
9001 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc gccttcgccc
9061 tcagacgagt cggatctccc tttgggccgc ctccccgcct gtccggatgg aagggctaat
9121 tcactcccaa cgaatacaag atctgctttt tgcttgtact gggtctctct ggttagacca
9181 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
9241 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
9301 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
9361 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
9421 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
9481 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
9541 ctggcatcta tgtcgggtgc ggagaaagag gtaatgaaat ggcattatgg gtattatggg
9601 tctgcattaa tgaatcggcc aacgatcccg gtgtgaaata ccgcacagat gcgtaaggag
9661 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
9721 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
9781 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
9841 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
9901 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
9961 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
10021 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
10081 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
10141 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
10201 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
10261 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
10321 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
10381 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
10441 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
10501 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
10561 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
10621 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
10681 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
10741 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
10801 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
10861 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
10921 cgttgttgaa aaaggatctt cacctagatc cttttcacgt agaaagccag tccgcagaaa
10981 cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc
11041 gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt
11101 ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag
11161 ccctgcaaag taaactggat ggctttctcg ccgccaagga tctgatggcg caggggatca
11221 agctctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
11281 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
11341 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
11401 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg
11461 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
11521 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
11581 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
11641 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
11701 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
11761 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat
11821 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
11881 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
11941 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
12001 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg aattttgtta
12061 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca acatccctta
12121 taaatcaaaa gaatagaccg cgatagggtt gagtgttgtt ccagtttgga acaagagtcc
12181 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
12241 cccactacgt gaaccatcac ccaaatcaag ttttttgcgg tcgaggtgcc gtaaagctct
12301 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
12361 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
12421 ggtcacgctg cgcgtaacca ccacacccgc gcgcttaatg cgccgctaca gggcgcgtcc
12481 attcgccatt caggatcgaa ttaattctta attaacatca tcaataatat acctt
LOCUS dna 12535 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
ORF 2381..3361
/sequence="ORF_5 rf(4)"
Promoter 2444..3621
/gene="EF1a prom"
ORF 3433..4284
/sequence="ORF_7 rf(5)"
ORF 3670..7101
/sequence="ORF_2 rf(1)"
ORF 4705..5925
/sequence="ORF_6 rf(5)"
misc_binding 7108..7113
/dbxref="REBASE:XbaI"
Promoter 7170..7438
/gene="SV40 prom"
Rep_Origin 7337..7414
/gene="SV40 origin"
ORF 7398..8231
/sequence="ORF_8 rf(6)"
ORF 7404..8498
/sequence="ORF_4 rf(3)"
misc_binding 7417..7429
/dbxref="REBASE:SfiI"
Marker 7478..8495
/gene="hygroB marker"
misc_binding 7716..7721
/dbxref="REBASE:EcoRI"
misc_binding 7922..7927
/dbxref="REBASE:NdeI"
Other Gene 9108..9160
/gene="delta_U3 other"
Other Gene 9161..9341
/gene="HIV-1_5_LTR other"
Rep_Origin 9847..10466
/gene="pBR322 origin"
Promoter 11119..11168
/gene="NEOKAN prom"
misc_binding 11226..11231
/dbxref="REBASE:BclI"
ORF 11257..12051
/sequence="ORF_3 rf(1)"
Marker 11260..12048
/gene="NTP_II marker"
Rep_Origin 12156..12461
/gene="f1 origin"