pLenti-Easy-His
| Vector Name | pLenti-Easy-His |
| Additional Information | No Additional Information |
Double-click on the features for more information
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgtgggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaacacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacaggaagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagtaagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttgtgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggtagcgaaagctcagatctggatctcccgatcccctatggtcgactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcgccgctgatcttcagacctggaggaggagatatgagggacaattgagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcgataagcttgggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccggctagcgtttaaacttaagcttggtaccggatccactagtgaattctctagagcggccgcaccgcggagtactgggccccctaggctcgagcatcatcaccatcaccattgatgatccggctgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctagcgaccagatctaatgcgtgctagcacgcattagatcggtcgctagcgaccagatctaatgcgtgctagcgaccagatctaatgcgtgctaggcttttgcaaaaagcttgggctgcaggtcgaggcggatctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgagcgggactctggggttcgatatctagcgaccagatctaattgcgtgcaaggcagctgtagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactcccaacgaagacaagatctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgggcctgaccagatctaatgcgtaggccgtttaaaccgctgatcagcctcgactgtgccttctagttgccagccatctgttgttgcccgggcgcgatcgctgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggcttctgaggcggaaagaaccagcagatcgatctgcatctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtattatgggtctgcattaatgaatcggccaacgatcccggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactggatggctttctcgccgccaaggatctgatggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttgttaaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatcccttataaatcaaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggatcgaattaattcttaattaacatcatcaataatatacctt
FEATURES Location/Qualifiers
source 1..8164
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 880..1060
/note="5' LTR"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
/note="color: #ffe4c4"
misc_feature 1107..1232
/note="HIV-1 psi"
/note="packaging signal of human immunodeficiency virus
type 1"
/note="color: #ffe4c4"
misc_feature 1723..1956
/note="HIV-1 RRE"
/note="The Rev response element (RRE) of human
immunodeficiency virus type 1 allows for Rev-dependent mRNA
export from the nucleus to the cytoplasm."
/note="color: #ffe4c4"
enhancer 2462..2767
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
/note="color: #ffffff"
promoter 2768..2971
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
/note="color: #ffffff; direction: RIGHT"
CDS 3131..3148
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/note="color: #cc99b2"
/translation="HHHHHH"
rep_origin 3349..3484
/note="SV40 ori"
/note="SV40 origin of replication"
/note="color: #ffff00"
CDS 3649..4443
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
/note="color: #ccffcc"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature 4537..4770
/note="3'LTR (Delta-U3)"
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
/note="color: #ffe4c4"
LTR 4590..4770
/note="5' LTR"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
/note="color: #ffe4c4"
rep_origin complement(5491..6079)
/direction=LEFT
/note="ori"
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
CDS 6886..7680
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
/note="color: #ccffcc"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc
541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt aatgtagtct
661 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
721 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
781 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
841 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
901 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
961 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
1021 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
1081 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
1141 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
1201 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
1261 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
1321 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
1381 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
1441 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
1501 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
1561 cacagcaagc gccgctgatc ttcagacctg gaggaggaga tatgagggac aattgagaag
1621 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1681 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1741 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1801 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1861 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1921 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1981 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
2041 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
2101 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
2161 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
2221 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
2281 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
2341 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
2401 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgataag cttgggagtt
2461 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
2521 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
2581 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
2641 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
2701 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
2761 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
2821 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
2881 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
2941 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
3001 acgccatcca cgctgttttg acctccatag aagacaccgg ctagcgttta aacttaagct
3061 tggtaccgga tccactagtg aattctctag agcggccgca ccgcggagta ctgggccccc
3121 taggctcgag catcatcacc atcaccattg atgatccggc tgtggaatgt gtgtcagtta
3181 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
3241 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
3301 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
3361 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
3421 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
3481 ggcctagcga ccagatctaa tgcgtgctag cacgcattag atcggtcgct agcgaccaga
3541 tctaatgcgt gctagcgacc agatctaatg cgtgctaggc ttttgcaaaa agcttgggct
3601 gcaggtcgag gcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
3661 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
3721 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
3781 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
3841 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
3901 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
3961 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
4021 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
4081 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
4141 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
4201 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
4261 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
4321 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
4381 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
4441 tgagcgggac tctggggttc gatatctagc gaccagatct aattgcgtgc aaggcagctg
4501 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
4561 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
4621 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
4681 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
4741 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
4801 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgggc ctgaccagat
4861 ctaatgcgta ggccgtttaa accgctgatc agcctcgact gtgccttcta gttgccagcc
4921 atctgttgtt gcccgggcgc gatcgctgcc cctcccccgt gccttccttg accctggaag
4981 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
5041 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
5101 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
5161 gcagatcgat ctgcatctat gtcgggtgcg gagaaagagg taatgaaatg gcattatggg
5221 tattatgggt ctgcattaat gaatcggcca acgatcccgg tgtgaaatac cgcacagatg
5281 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
5341 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
5401 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
5461 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
5521 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
5581 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
5641 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
5701 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
5761 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
5821 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
5881 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
5941 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
6001 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
6061 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
6121 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
6181 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
6241 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
6301 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
6361 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
6421 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
6481 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
6541 tttgcgcaac gttgttgaaa aaggatcttc acctagatcc ttttcacgta gaaagccagt
6601 ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa
6661 aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac
6721 tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag
6781 gttgggaagc cctgcaaagt aaactggatg gctttctcgc cgccaaggat ctgatggcgc
6841 aggggatcaa gctctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
6901 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
6961 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
7021 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
7081 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
7141 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
7201 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
7261 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
7321 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
7381 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
7441 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
7501 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
7561 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
7621 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
7681 attttgttaa aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
7741 catcccttat aaatcaaaag aatagaccgc gatagggttg agtgttgttc cagtttggaa
7801 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
7861 gggcgatggc ccactacgtg aaccatcacc caaatcaagt tttttgcggt cgaggtgccg
7921 taaagctcta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
7981 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
8041 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcg cgcttaatgc gccgctacag
8101 ggcgcgtcca ttcgccattc aggatcgaat taattcttaa ttaacatcat caataatata
8161 cctt
//