pLenti-GIII-CMV-DIO-SV40-GFP

                            LOCUS       dna                     10314 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    457..462
                     /dbxref="REBASE:HpaI"
     Other Gene      715..895
                     /gene="HIV-1_5_LTR other"
     Other Gene      1006..1050
                     /gene="HIV-1_psi_pack other"
     misc_binding    1092..1097
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  1557..1790
                     /gene="RRE reg"
     ORF             1600..2301
                     /sequence="ORF_1 rf(1)"
     misc_binding    2611..2616
                     /dbxref="REBASE:NdeI"
     Promoter        2885..2966
                     /gene="CMV prom"
     Regulatory_Seq  3022..3061
                     /gene="tetO reg"
     misc_binding    3074..3079
                     /dbxref="REBASE:ClaI"
     misc_binding    3098..3103
                     /dbxref="REBASE:KpnI"
     Other Gene      3218..3251
                     /gene="loxP other"
     Other Gene      3273..3392
                     /gene="attR1 other"
     Other Gene      3292..3392
                     /gene="attR2 other"
     Other Gene      3433..3738
                     /gene="ccdB other"
     Marker          4080..4739
                     /gene="CAT marker"
     ORF             4080..4739
                     /sequence="ORF_4 rf(5)"
     misc_binding    4521..4526
                     /dbxref="REBASE:EcoRI"
     misc_binding    4840..4847
                     /dbxref="REBASE:NotI"
     Other Gene      4848..4972
                     /gene="attR1 other"
     Other Gene      4848..4948
                     /gene="attR2 other"
     Other Gene      5096..5129
                     /gene="loxP other"
     misc_binding    5148..5153
                     /dbxref="REBASE:XbaI"
     Promoter        5210..5478
                     /gene="SV40 prom"
     Rep_Origin      5377..5454
                     /gene="SV40 origin"
     ORF             5444..6277
                     /sequence="ORF_2 rf(2)"
     misc_binding    5457..5469
                     /dbxref="REBASE:SfiI"
     misc_binding    6103..6108
                     /dbxref="REBASE:ApaI"
     Other Gene      6887..6939
                     /gene="delta_U3 other"
     Other Gene      6940..7120
                     /gene="HIV-1_5_LTR other"
     Rep_Origin      7626..8245
                     /gene="pBR322 origin"
     Promoter        8898..8947
                     /gene="NEOKAN prom"
     misc_binding    9005..9010
                     /dbxref="REBASE:BclI"
     ORF             9036..9830
                     /sequence="ORF_3 rf(3)"
     Marker          9039..9827
                     /gene="NTP_II marker"
     Rep_Origin      9935..10240
                     /gene="f1 origin"
BASE COUNT    2698 a   2516 c   2603 g   2497 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta     
      481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag     
      541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg     
      601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact     
      661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct     
      721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt     
      781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac     
      841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc     
      901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc     
      961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa     
     1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg     
     1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata     
     1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc     
     1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga     
     1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc     
     1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca     
     1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag     
     1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta     
     1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga     
     1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg     
     1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg     
     1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc     
     1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg     
     1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat     
     1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac     
     1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat     
     1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca     
     2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga     
     2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg     
     2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa     
     2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga     
     2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag     
     2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa     
     2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat     
     2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt     
     2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa     
     2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc     
     2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct     
     2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt     
     2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg     
     2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca     
     2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca     
     2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc     
     3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag     
     3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accaatcagc tcgctcacca     
     3121 tataacttcg tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc     
     3181 accatcgacc cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta     
     3241 tacgaagtta tctcgagtca gatatcaacc actttgtaca agaaagctga acgagaaacg     
     3301 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa     
     3361 tactgtaaaa cacaacatat ccagtcacta tggtcgacct gcagactggc tgtgtataag     
     3421 ggagcctgac atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc     
     3481 gcggtggctg agatcagcca cttcttcccc gataacggag accggcacac tggccatatc     
     3541 ggtggtcatc atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga     
     3601 gactttatct gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt     
     3661 gtcaataata tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt     
     3721 gtaaacctta aactgcattt caccagcccc tgttctcgtc agcaaaagag ccgttcattt     
     3781 caataaaccg ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac     
     3841 gcagacgacg ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat     
     3901 atatgccttg agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat     
     3961 agcatacctc tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa     
     4021 aaatcagcgc gcaaatacgc atactgttat ctggctttta gtaagccgga tccagatctt     
     4081 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca     
     4141 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg     
     4201 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc     
     4261 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc     
     4321 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa     
     4381 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt     
     4441 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca     
     4501 ccgtctttca ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga     
     4561 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata     
     4621 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt     
     4681 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt     
     4741 ttagcttcct tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac     
     4801 gagccggaag cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga     
     4861 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat     
     4921 tgatatttat atcattttac gtttctcgtt cagctttttt gtacaaactt gttgatatcg     
     4981 ctagccaatt gataacttcg tataggatac tttatacgaa gttatcattg ggattcttcc     
     5041 tattttgatc caagcatcac catcgaccct ctagtccaga tctcaccatc gacccataac     
     5101 ttcgtatagc atacattata cgaagttata tggtgagcaa gggcgattct agactgcagc     
     5161 tcgagtaccc atacgacgtc ccagactacg cttgagttta aacacgcgtg gtgtggaaag     
     5221 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc     
     5281 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat     
     5341 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt     
     5401 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc     
     5461 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg ccgccaccat     
     5521 ggagagcgac gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct     
     5581 gaacggcgtg gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat     
     5641 gaccaacaag atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca     
     5701 cgtgatgggc tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt     
     5761 cctgcacgcc atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg     
     5821 cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt     
     5881 caaggtggtg ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg     
     5941 cagcaacgcc accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt     
     6001 cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat     
     6061 gcacttcaag agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt     
     6121 ccgccgcgtg gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc     
     6181 cttcaagacc cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga     
     6241 cggcaccgcc ggacccggct ccaccggatc tcgctgaacg cgttccggaa atcaacctct     
     6301 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct     
     6361 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat     
     6421 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt     
     6481 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat     
     6541 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc     
     6601 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga     
     6661 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc     
     6721 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga     
     6781 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcg ccttcgccct     
     6841 cagacgagtc ggatctccct ttgggccgcc tccccgcctg tccggatgga agggctaatt     
     6901 cactcccaac gaatacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag     
     6961 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc     
     7021 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga     
     7081 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt     
     7141 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt     
     7201 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca     
     7261 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc     
     7321 tggcatctat gtcgggtgcg gagaaagagg taatgaaatg gcattatggg tattatgggt     
     7381 ctgcattaat gaatcggcca acgatcccgg tgtgaaatac cgcacagatg cgtaaggaga     
     7441 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt     
     7501 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca     
     7561 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa     
     7621 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat     
     7681 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc     
     7741 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc     
     7801 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt     
     7861 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac     
     7921 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg     
     7981 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca     
     8041 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc     
     8101 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa     
     8161 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa     
     8221 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac     
     8281 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta     
     8341 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt     
     8401 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata     
     8461 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc     
     8521 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac     
     8581 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag     
     8641 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac     
     8701 gttgttgaaa aaggatcttc acctagatcc ttttcacgta gaaagccagt ccgcagaaac     
     8761 ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg     
     8821 caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt     
     8881 tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc     
     8941 cctgcaaagt aaactggatg gctttctcgc cgccaaggat ctgatggcgc aggggatcaa     
     9001 gctctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg     
     9061 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa     
     9121 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg     
     9181 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt     
     9241 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa     
     9301 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc     
     9361 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg     
     9421 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg     
     9481 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg     
     9541 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg     
     9601 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact     
     9661 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg     
     9721 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc     
     9781 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attttgttaa     
     9841 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa catcccttat     
     9901 aaatcaaaag aatagaccgc gatagggttg agtgttgttc cagtttggaa caagagtcca     
     9961 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc     
    10021 ccactacgtg aaccatcacc caaatcaagt tttttgcggt cgaggtgccg taaagctcta     
    10081 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg     
    10141 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg     
    10201 gtcacgctgc gcgtaaccac cacacccgcg cgcttaatgc gccgctacag ggcgcgtcca     
    10261 ttcgccattc aggatcgaat taattcttaa ttaacatcat caataatata cctt           
//