pLenti-GIII-CMV-DIO-SV40-Puro

                            LOCUS       dna                     10149 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    457..462
                     /dbxref="REBASE:HpaI"
     Other Gene      715..895
                     /gene="HIV-1_5_LTR other"
     Other Gene      1006..1050
                     /gene="HIV-1_psi_pack other"
     misc_binding    1092..1097
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  1557..1790
                     /gene="RRE reg"
     ORF             1600..2301
                     /sequence="ORF_1 rf(1)"
     misc_binding    2611..2616
                     /dbxref="REBASE:NdeI"
     Promoter        2885..2966
                     /gene="CMV prom"
     Regulatory_Seq  3022..3061
                     /gene="tetO reg"
     misc_binding    3074..3079
                     /dbxref="REBASE:ClaI"
     misc_binding    3098..3103
                     /dbxref="REBASE:KpnI"
     Other Gene      3218..3251
                     /gene="loxP other"
     Other Gene      3273..3392
                     /gene="attR1 other"
     Other Gene      3292..3392
                     /gene="attR2 other"
     Other Gene      3433..3738
                     /gene="ccdB other"
     Marker          4080..4739
                     /gene="CAT marker"
     ORF             4080..4739
                     /sequence="ORF_4 rf(5)"
     misc_binding    4521..4526
                     /dbxref="REBASE:EcoRI"
     misc_binding    4840..4847
                     /dbxref="REBASE:NotI"
     Other Gene      4848..4972
                     /gene="attR1 other"
     Other Gene      4848..4948
                     /gene="attR2 other"
     Other Gene      5096..5129
                     /gene="loxP other"
     misc_binding    5148..5153
                     /dbxref="REBASE:XbaI"
     Promoter        5210..5478
                     /gene="SV40 prom"
     Rep_Origin      5377..5454
                     /gene="SV40 origin"
     ORF             5444..6112
                     /sequence="ORF_2 rf(2)"
     misc_binding    5457..5469
                     /dbxref="REBASE:SfiI"
     Marker          5513..6112
                     /gene="puro marker"
     Other Gene      6722..6774
                     /gene="delta_U3 other"
     Other Gene      6775..6955
                     /gene="HIV-1_5_LTR other"
     Rep_Origin      7461..8080
                     /gene="pBR322 origin"
     Promoter        8733..8782
                     /gene="NEOKAN prom"
     misc_binding    8840..8845
                     /dbxref="REBASE:BclI"
     ORF             8871..9665
                     /sequence="ORF_3 rf(3)"
     Marker          8874..9662
                     /gene="NTP_II marker"
     Rep_Origin      9770..10075
                     /gene="f1 origin"
BASE COUNT    2629 a   2473 c   2585 g   2462 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta     
      481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag     
      541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg     
      601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact     
      661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct     
      721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt     
      781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac     
      841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc     
      901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc     
      961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa     
     1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg     
     1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata     
     1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc     
     1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga     
     1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc     
     1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca     
     1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag     
     1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta     
     1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga     
     1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg     
     1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg     
     1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc     
     1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg     
     1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat     
     1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac     
     1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat     
     1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca     
     2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga     
     2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg     
     2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa     
     2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga     
     2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag     
     2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa     
     2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat     
     2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt     
     2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa     
     2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc     
     2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct     
     2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt     
     2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg     
     2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca     
     2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca     
     2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc     
     3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag     
     3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accaatcagc tcgctcacca     
     3121 tataacttcg tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc     
     3181 accatcgacc cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta     
     3241 tacgaagtta tctcgagtca gatatcaacc actttgtaca agaaagctga acgagaaacg     
     3301 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa     
     3361 tactgtaaaa cacaacatat ccagtcacta tggtcgacct gcagactggc tgtgtataag     
     3421 ggagcctgac atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc     
     3481 gcggtggctg agatcagcca cttcttcccc gataacggag accggcacac tggccatatc     
     3541 ggtggtcatc atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga     
     3601 gactttatct gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt     
     3661 gtcaataata tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt     
     3721 gtaaacctta aactgcattt caccagcccc tgttctcgtc agcaaaagag ccgttcattt     
     3781 caataaaccg ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac     
     3841 gcagacgacg ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat     
     3901 atatgccttg agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat     
     3961 agcatacctc tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa     
     4021 aaatcagcgc gcaaatacgc atactgttat ctggctttta gtaagccgga tccagatctt     
     4081 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca     
     4141 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg     
     4201 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc     
     4261 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc     
     4321 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa     
     4381 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt     
     4441 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca     
     4501 ccgtctttca ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga     
     4561 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata     
     4621 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt     
     4681 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt     
     4741 ttagcttcct tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac     
     4801 gagccggaag cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga     
     4861 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat     
     4921 tgatatttat atcattttac gtttctcgtt cagctttttt gtacaaactt gttgatatcg     
     4981 ctagccaatt gataacttcg tataggatac tttatacgaa gttatcattg ggattcttcc     
     5041 tattttgatc caagcatcac catcgaccct ctagtccaga tctcaccatc gacccataac     
     5101 ttcgtatagc atacattata cgaagttata tggtgagcaa gggcgattct agactgcagc     
     5161 tcgagtaccc atacgacgtc ccagactacg cttgagttta aacacgcgtg gtgtggaaag     
     5221 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc     
     5281 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat     
     5341 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt     
     5401 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc     
     5461 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg ccatgaccga     
     5521 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cctcgggccg tacgcaccct     
     5581 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtggacccgg accgccacat     
     5641 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa     
     5701 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga     
     5761 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct     
     5821 ggccgcgcag caacagatgg aagggctcct ggcgccgcac cggcccaagg agcccgcgtg     
     5881 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt     
     5941 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc     
     6001 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga     
     6061 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaacgcgttc     
     6121 cggaaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt     
     6181 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc     
     6241 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga     
     6301 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc     
     6361 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct     
     6421 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg     
     6481 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct     
     6541 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc     
     6601 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg     
     6661 tctcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgtccgg     
     6721 atggaagggc taattcactc ccaacgaata caagatctgc tttttgcttg tactgggtct     
     6781 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt     
     6841 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac     
     6901 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt     
     6961 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt     
     7021 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat     
     7081 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat     
     7141 gtatcttatc atgtctggca tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt     
     7201 atgggtatta tgggtctgca ttaatgaatc ggccaacgat cccggtgtga aataccgcac     
     7261 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg     
     7321 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg     
     7381 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag     
     7441 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac     
     7501 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga     
     7561 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt     
     7621 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc     
     7681 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc     
     7741 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta     
     7801 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat     
     7861 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca     
     7921 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct     
     7981 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt     
     8041 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct     
     8101 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc     
     8161 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa     
     8221 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta     
     8281 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc     
     8341 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat     
     8401 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta     
     8461 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt     
     8521 aatagtttgc gcaacgttgt tgaaaaagga tcttcaccta gatccttttc acgtagaaag     
     8581 ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa     
     8641 gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc     
     8701 tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg     
     8761 gtaaggttgg gaagccctgc aaagtaaact ggatggcttt ctcgccgcca aggatctgat     
     8821 ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc atgattgaac     
     8881 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact     
     8941 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc     
     9001 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg     
     9061 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg     
     9121 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt     
     9181 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc     
     9241 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag     
     9301 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg     
     9361 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc     
     9421 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt     
     9481 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg     
     9541 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt     
     9601 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct     
     9661 tctgaatttt gttaaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc     
     9721 ggcaacatcc cttataaatc aaaagaatag accgcgatag ggttgagtgt tgttccagtt     
     9781 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc     
     9841 tatcagggcg atggcccact acgtgaacca tcacccaaat caagtttttt gcggtcgagg     
     9901 tgccgtaaag ctctaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga     
     9961 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg     
    10021 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc     
    10081 tacagggcgc gtccattcgc cattcaggat cgaattaatt cttaattaac atcatcaata     
    10141 atatacctt                                                             
//