pLenti-HOXA10-RFP
Vector Name | pLenti-HOXA10-RFP |
VectorType | Lentiviral Vector |
Antibiotic Information | No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
Additional Information | No Additional Information |
Double-click on the features for more information
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcat gtcagccaga aagggctatc 3121 tgctcccttc gccaaattat cccacaacaa tgtcatgctc ggagagcccc gccgcgaact 3181 cttttttggt cgactcgctc atcagctcgg gcagaggcga ggcaggcggc ggtggtggtg 3241 gcgcgggggg cggcggcggt ggcggttact acgcccacgg cggggtctac ctgccgcccg 3301 ccgccgacct gccctacggg ctgcagagct gcgggctctt ccccacgctg ggcggcaagc 3361 gcaatgaggc agcgtcgccg ggcagcggtg gcggtggcgg gggtctaggt cccggggcgc 3421 acggctacgg gccctcgccc atagacctgt ggctagacgc gccccggtct tgccggatgg 3481 agccgcctga cgggccgccg ccgccgcccc agcagcagcc gccgcccccg ccgcaaccac 3541 cccagccagc gccgcaggcc acctcgtgct ctttcgcgca gaacatcaaa gaagagagct 3601 cctactgcct ctacgactcg gcggacaaat gccccaaagt ctcggccacc gccgccgaac 3661 tggctccctt cccgcggggc ccgccgcccg acggctgcgc cctgggcacc tccagcgggg 3721 tgccagtgcc tggctacttc cgcctttctc aggcctacgg caccgccaag ggctatggca 3781 gcggcggcgg cggcgcgcag caactcgggg ctggcccgtt ccccgcgcag cccccggggc 3841 gcggtttcga tctcccgccc gcgctagcct ccggctcggc cgatgcggcc cggaaggagc 3901 gagccctcga ttcgccgccg ccccccacgc tggcttgcgg cagcggcggg ggctcgcagg 3961 gcgacgagga ggcgcacgcg tcgtcctcgg ccgcggagga gctctccccg gccccttccg 4021 agagcagcaa agcctcgccg gagaaggatt ccctgggcaa ttccaaaggt gaaaacgcag 4081 ccaactggct cacggcaaag agtggtcgga agaagcgctg cccctacacg aagcaccaga 4141 cactggagct ggagaaggag tttctgttca atatgtacct tactcgagag cggcgcctag 4201 agattagccg cagcgtccac ctcacggaca gacaagtgaa aatctggttt cagaaccgca 4261 ggatgaaact gaagaaaatg aatcgagaaa accggatccg ggagctcaca gccaacttta 4321 atttttcctg cccaactttc ttgtacaaag tggttgatat ctgactcgag tgaatgaatg 4381 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 4441 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 4501 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 4561 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 4621 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 4681 ggccactagt gtcgagaaaa ctagtcgcca ccatggtgag cgagctgatt aaggagaaca 4741 tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag tgcacatccg 4801 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggcg gtcgagggcg 4861 gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc agcaaaacct 4921 tcatcaacca cacccagggc atccccgact tctttaagca gtccttcccc gagggcttca 4981 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca 5041 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcccatcca 5101 acggccctgt gatgcagaag aaaacactcg gctgggaggc ctccaccgag accctgtacc 5161 ccgctgacgg cggcctggaa ggcagagccg acatggccct gaagctcgtg ggcgggggcc 5221 acctgatctg caacttgaag accacataca gatccaagaa acccgctaag aacctcaaga 5281 tgcccggcgt ctactatgtg gacagaagac tggaaagaat caaggaggcc gacaaagaga 5341 cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg 5401 ggcacagaga attcggaagc gggcagtgca ccaactacgc cctgctgaag ctggccggcg 5461 acgtggagag caaccccggc cccggatcca tggccaccga gtacaagccc acggtgcgcc 5521 tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact 5581 accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc 5641 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 5701 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 5761 agatcggctc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 5821 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 5881 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 5941 cggccgagcg cgctggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 6001 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca 6061 cctggtgcat gacccgcaag cccggtgcct gaacgcgttc cggaaatcaa cctctggatt 6121 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 6181 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 6241 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 6301 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 6361 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 6421 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 6481 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct 6541 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 6601 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctcgccttc gccctcagac 6661 gagtcggatc tccctttggg ccgcctcccc gcctgtccgg atggaagggc taattcactc 6721 ccaacgaata caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg 6781 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 6841 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 6901 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 6961 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc 7021 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 7081 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggca 7141 tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt atgggtatta tgggtctgca 7201 ttaatgaatc ggccaacgat cccggtgtga aataccgcac agatgcgtaa ggagaaaata 7261 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 7321 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 7381 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 7441 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 7501 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 7561 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 7621 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 7681 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 7741 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 7801 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 7861 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 7921 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 7981 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 8041 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 8101 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 8161 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 8221 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 8281 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 8341 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 8401 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 8461 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 8521 tgaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc 8581 tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag 8641 agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg 8701 acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc 8761 aaagtaaact ggatggcttt ctcgccgcca aggatctgat ggcgcagggg atcaagctct 8821 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt 8881 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc 8941 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag 9001 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg 9061 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac 9121 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc 9181 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc 9241 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc 9301 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg 9361 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat 9421 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc 9481 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa 9541 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat 9601 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaatttt gttaaaattt 9661 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaacatcc cttataaatc 9721 aaaagaatag accgcgatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt 9781 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact 9841 acgtgaacca tcacccaaat caagtttttt gcggtcgagg tgccgtaaag ctctaaatcg 9901 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag 9961 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac 10021 gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc tacagggcgc gtccattcgc 10081 cattcaggat cgaattaatt cttaattaac atcatcaata atatacctt
LOCUS dna 10129 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" misc_binding 1092..1097 /dbxref="REBASE:NruI" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" misc_binding 2611..2616 /dbxref="REBASE:NdeI" Promoter 2885..2966 /gene="CMV prom" ORF 2901..4364 /sequence="ORF_3 rf(3)" Regulatory_Seq 3022..3061 /gene="tetO reg" misc_binding 3321..3326 /dbxref="REBASE:PstI" Promoter 4382..4650 /gene="SV40 prom" Rep_Origin 4549..4626 /gene="SV40 origin" ORF 4713..6092 /sequence="ORF_4 rf(3)" misc_binding 5409..5414 /dbxref="REBASE:EcoRI" Marker 5496..6092 /gene="puro marker" Other Gene 6702..6754 /gene="delta_U3 other" Other Gene 6755..6935 /gene="HIV-1_5_LTR other" Rep_Origin 7441..8060 /gene="pBR322 origin" Promoter 8713..8762 /gene="NEOKAN prom" misc_binding 8820..8825 /dbxref="REBASE:BclI" ORF 8851..9645 /sequence="ORF_2 rf(1)" Marker 8854..9642 /gene="NTP_II marker" Rep_Origin 9750..10055 /gene="f1 origin" BASE COUNT 2477 a 2665 c 2804 g 2183 t 0 others ORIGIN 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcat gtcagccaga aagggctatc 3121 tgctcccttc gccaaattat cccacaacaa tgtcatgctc ggagagcccc gccgcgaact 3181 cttttttggt cgactcgctc atcagctcgg gcagaggcga ggcaggcggc ggtggtggtg 3241 gcgcgggggg cggcggcggt ggcggttact acgcccacgg cggggtctac ctgccgcccg 3301 ccgccgacct gccctacggg ctgcagagct gcgggctctt ccccacgctg ggcggcaagc 3361 gcaatgaggc agcgtcgccg ggcagcggtg gcggtggcgg gggtctaggt cccggggcgc 3421 acggctacgg gccctcgccc atagacctgt ggctagacgc gccccggtct tgccggatgg 3481 agccgcctga cgggccgccg ccgccgcccc agcagcagcc gccgcccccg ccgcaaccac 3541 cccagccagc gccgcaggcc acctcgtgct ctttcgcgca gaacatcaaa gaagagagct 3601 cctactgcct ctacgactcg gcggacaaat gccccaaagt ctcggccacc gccgccgaac 3661 tggctccctt cccgcggggc ccgccgcccg acggctgcgc cctgggcacc tccagcgggg 3721 tgccagtgcc tggctacttc cgcctttctc aggcctacgg caccgccaag ggctatggca 3781 gcggcggcgg cggcgcgcag caactcgggg ctggcccgtt ccccgcgcag cccccggggc 3841 gcggtttcga tctcccgccc gcgctagcct ccggctcggc cgatgcggcc cggaaggagc 3901 gagccctcga ttcgccgccg ccccccacgc tggcttgcgg cagcggcggg ggctcgcagg 3961 gcgacgagga ggcgcacgcg tcgtcctcgg ccgcggagga gctctccccg gccccttccg 4021 agagcagcaa agcctcgccg gagaaggatt ccctgggcaa ttccaaaggt gaaaacgcag 4081 ccaactggct cacggcaaag agtggtcgga agaagcgctg cccctacacg aagcaccaga 4141 cactggagct ggagaaggag tttctgttca atatgtacct tactcgagag cggcgcctag 4201 agattagccg cagcgtccac ctcacggaca gacaagtgaa aatctggttt cagaaccgca 4261 ggatgaaact gaagaaaatg aatcgagaaa accggatccg ggagctcaca gccaacttta 4321 atttttcctg cccaactttc ttgtacaaag tggttgatat ctgactcgag tgaatgaatg 4381 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 4441 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 4501 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 4561 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 4621 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 4681 ggccactagt gtcgagaaaa ctagtcgcca ccatggtgag cgagctgatt aaggagaaca 4741 tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag tgcacatccg 4801 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggcg gtcgagggcg 4861 gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc agcaaaacct 4921 tcatcaacca cacccagggc atccccgact tctttaagca gtccttcccc gagggcttca 4981 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca 5041 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcccatcca 5101 acggccctgt gatgcagaag aaaacactcg gctgggaggc ctccaccgag accctgtacc 5161 ccgctgacgg cggcctggaa ggcagagccg acatggccct gaagctcgtg ggcgggggcc 5221 acctgatctg caacttgaag accacataca gatccaagaa acccgctaag aacctcaaga 5281 tgcccggcgt ctactatgtg gacagaagac tggaaagaat caaggaggcc gacaaagaga 5341 cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg 5401 ggcacagaga attcggaagc gggcagtgca ccaactacgc cctgctgaag ctggccggcg 5461 acgtggagag caaccccggc cccggatcca tggccaccga gtacaagccc acggtgcgcc 5521 tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact 5581 accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc 5641 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 5701 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 5761 agatcggctc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 5821 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 5881 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 5941 cggccgagcg cgctggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 6001 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca 6061 cctggtgcat gacccgcaag cccggtgcct gaacgcgttc cggaaatcaa cctctggatt 6121 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 6181 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 6241 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 6301 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 6361 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 6421 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 6481 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct 6541 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 6601 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctcgccttc gccctcagac 6661 gagtcggatc tccctttggg ccgcctcccc gcctgtccgg atggaagggc taattcactc 6721 ccaacgaata caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg 6781 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 6841 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 6901 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 6961 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc 7021 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 7081 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggca 7141 tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt atgggtatta tgggtctgca 7201 ttaatgaatc ggccaacgat cccggtgtga aataccgcac agatgcgtaa ggagaaaata 7261 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 7321 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 7381 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 7441 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 7501 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 7561 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 7621 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 7681 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 7741 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 7801 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 7861 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 7921 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 7981 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 8041 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 8101 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 8161 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 8221 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 8281 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 8341 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 8401 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 8461 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 8521 tgaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc 8581 tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag 8641 agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg 8701 acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc 8761 aaagtaaact ggatggcttt ctcgccgcca aggatctgat ggcgcagggg atcaagctct 8821 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt 8881 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc 8941 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag 9001 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg 9061 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac 9121 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc 9181 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc 9241 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc 9301 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg 9361 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat 9421 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc 9481 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa 9541 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat 9601 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaatttt gttaaaattt 9661 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaacatcc cttataaatc 9721 aaaagaatag accgcgatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt 9781 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact 9841 acgtgaacca tcacccaaat caagtttttt gcggtcgagg tgccgtaaag ctctaaatcg 9901 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag 9961 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac 10021 gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc tacagggcgc gtccattcgc 10081 cattcaggat cgaattaatt cttaattaac atcatcaata atatacctt //