pLenti-III-2A-GFP

                            LOCUS       dna                      8511 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      109..289
                     /gene="HIV-1_5_LTR other"
     Other Gene      400..444
                     /gene="HIV-1_psi_pack other"
     misc_binding    486..491
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  951..1184
                     /gene="RRE reg"
     ORF             994..1695
                     /sequence="ORF_1 rf(1)"
     misc_binding    2005..2010
                     /dbxref="REBASE:NdeI"
     Promoter        2279..2360
                     /gene="CMV prom"
     Regulatory_Seq  2416..2455
                     /gene="tetO reg"
     misc_binding    2462..2467
                     /dbxref="REBASE:XmaI"
     misc_binding    2462..2467
                     /dbxref="REBASE:SmaI"
     misc_binding    2468..2473
                     /dbxref="REBASE:ClaI"
     misc_binding    2492..2497
                     /dbxref="REBASE:KpnI"
     misc_binding    2511..2516
                     /dbxref="REBASE:PstI"
     misc_binding    2519..2524
                     /dbxref="REBASE:EcoRI"
     misc_binding    2537..2542
                     /dbxref="REBASE:StuI"
     misc_binding    2543..2548
                     /dbxref="REBASE:HincII"
     misc_binding    2549..2554
                     /dbxref="REBASE:EcoRV"
     misc_binding    2555..2560
                     /dbxref="REBASE:ApaI"
     misc_binding    2561..2568
                     /dbxref="REBASE:NotI"
     misc_binding    2570..2575
                     /dbxref="REBASE:BamHI"
     ORF             2600..3454
                     /sequence="ORF_5 rf(6)"
     Reporter        2636..3352
                     /gene="EGFP reporter"
     ORF             2636..3355
                     /sequence="ORF_4 rf(2)"
     misc_binding    3356..3361
                     /dbxref="REBASE:XhoI"
     Promoter        3406..3674
                     /gene="SV40 prom"
     Rep_Origin      3573..3650
                     /gene="SV40 origin"
     ORF             3640..4308
                     /sequence="ORF_2 rf(1)"
     misc_binding    3653..3665
                     /dbxref="REBASE:SfiI"
     Marker          3709..4308
                     /gene="puro marker"
     Other Gene      4918..4970
                     /gene="delta_U3 other"
     Other Gene      4971..5151
                     /gene="HIV-1_5_LTR other"
     Rep_Origin      5658..6277
                     /gene="pBR322 origin"
     Promoter        6535..6584
                     /gene="NEOKAN prom"
     misc_binding    6642..6647
                     /dbxref="REBASE:BclI"
     ORF             6673..7467
                     /sequence="ORF_3 rf(1)"
     Marker          6676..7464
                     /gene="NTP_II marker"
     Rep_Origin      7572..7878
                     /gene="f1 origin"
     Other Gene      7933..8034
                     /gene="RITR other"
     Other Gene      7933..8034
                     /gene="LITR other"
     Other Gene      8114..8262
                     /gene="Encap other"
BASE COUNT    2121 a   2157 c   2342 g   1891 t    0 others
ORIGIN
        1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg     
       61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg     
      121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct     
      181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt     
      241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga     
      301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg     
      361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga     
      421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa     
      481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa     
      541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag     
      601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat     
      661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga     
      721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta     
      781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat     
      841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     
      901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     
      961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     
     1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     
     1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     
     1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     
     1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct     
     1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     
     1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     
     1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     
     1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     
     1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     
     1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     
     1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt     
     1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt     
     1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca     
     1801 aaattttcgg gtttttcgaa cctagggttc cgcgttacat aacttacggt aaatggcccg     
     1861 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata     
     1921 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc     
     1981 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac     
     2041 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg     
     2101 cagtacatct acgtttagtc atcgctatta ccatggtgat gcggttttgg cagtacatca     
     2161 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca     
     2221 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg     
     2281 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc     
     2341 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa     
     2401 gaaccgagtt taaactccct atcagtgata gagatctccc tatcagtgat agagagctag     
     2461 ccccgggatc gatcaattga gtacttacgt aggtacccca gtgtggtggc ctgcaggtga     
     2521 attcactagt accggtaggc ctgtcgacga tatcgggccc gcggccgctg gatcctctag     
     2581 cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatggt     
     2641 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga     
     2701 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa     
     2761 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt     
     2821 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca     
     2881 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa     
     2941 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa     
     3001 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct     
     3061 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat     
     3121 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca     
     3181 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct     
     3241 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct     
     3301 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtagctcga     
     3361 gtacccatac gacgtcccag actacgcttg agtttaaaca cgcgtggtgt ggaaagtccc     
     3421 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt     
     3481 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt     
     3541 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg     
     3601 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct     
     3661 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccat gaccgagtac     
     3721 aagcccacgg tgcgcctcgc cacccgcgac gacgtccctc gggccgtacg caccctcgcc     
     3781 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtgg acccggaccg ccacatcgag     
     3841 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg     
     3901 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg     
     3961 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc     
     4021 gcgcagcaac agatggaagg gctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc     
     4081 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg     
     4141 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg     
     4201 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg     
     4261 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaac gcgttccgga     
     4321 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct     
     4381 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt     
     4441 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg     
     4501 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact     
     4561 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct     
     4621 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg     
     4681 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc     
     4741 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc     
     4801 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc     
     4861 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct gtccggatgg     
     4921 aagggctaat tcactcccaa cgaatacaag atctgctttt tgcttgtact gggtctctct     
     4981 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc     
     5041 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg     
     5101 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt     
     5161 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga     
     5221 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca     
     5281 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat     
     5341 cttatcatgt ctggcatcta tgtcgggtgc ggagaaagag gtaatgaaat ggcattatgg     
     5401 gtattatggg tctgcattaa tgaatcggcc aacggatgtc ggtgtgaaat accgcacaga     
     5461 tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg     
     5521 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta     
     5581 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc     
     5641 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag     
     5701 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac     
     5761 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc     
     5821 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt     
     5881 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc     
     5941 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga     
     6001 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta     
     6061 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta     
     6121 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga     
     6181 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg     
     6241 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag     
     6301 tggaacgaaa actcacgtta agggattttg gccatgagat tatcaaaaag gatcttcacc     
     6361 tagatccttt tcacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc     
     6421 agctactggg ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc     
     6481 agtgggctta catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa     
     6541 ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct     
     6601 ttcttgccgc caaggatctg atggcgcagg ggatcaagct ctgatcaaga gacaggatga     
     6661 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg     
     6721 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg     
     6781 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc     
     6841 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct     
     6901 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa     
     6961 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg     
     7021 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa     
     7081 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat     
     7141 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg     
     7201 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc     
     7261 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac     
     7321 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg     
     7381 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc     
     7441 tatcgccttc ttgacgagtt cttctgaatt ttgttaaaat ttttgttaaa tcagctcatt     
     7501 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat     
     7561 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa     
     7621 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta     
     7681 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc     
     7741 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc     
     7801 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac     
     7861 acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gatcgaatta     
     7921 attcttaatt aacatcatca ataatatacc ttttttggat tgaagccaat atgataatga     
     7981 gggggtggag tttgtgacgt ggcgcggggc gtgggaacgg ggcgggtgac gtagtagtgt     
     8041 ggcggaagtg tgatgttgca agtgtggcgg aacacatgta agcgacggat gtggcaaaag     
     8101 tgacgttttt ggtgtgcgcc ggtgtacaca ggaagtgaca attttcgcgc ggttttaggc     
     8161 ggatgttgta gtaaatttgg gcgtaaccga gtaagatttg gccattttcg cgggaaaact     
     8221 gaataagagg aagtgaaatc tgaataattt tgtgttactc atagcgcgta atactctcat     
     8281 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt     
     8341 tccccgaaaa gtgccacctg acgtaactat aacggtccta aggtagcgaa aatgtagtct     
     8401 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa     
     8461 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga t              
//