pLenti-III-EF1alpha

                            LOCUS       dna                      8311 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      109..289
                     /gene="HIV-1_5_LTR other"
     Other Gene      400..444
                     /gene="HIV-1_psi_pack other"
     misc_binding    486..491
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  951..1184
                     /gene="RRE reg"
     ORF             994..1695
                     /sequence="ORF_1 rf(1)"
     ORF             1775..2755
                     /sequence="ORF_4 rf(4)"
     Promoter        1838..3015
                     /gene="EF1a prom"
     misc_binding    3087..3092
                     /dbxref="REBASE:EcoRI"
     misc_binding    3111..3116
                     /dbxref="REBASE:HincII"
     misc_binding    3117..3122
                     /dbxref="REBASE:EcoRV"
     misc_binding    3129..3136
                     /dbxref="REBASE:NotI"
     misc_binding    3138..3143
                     /dbxref="REBASE:BamHI"
     misc_binding    3144..3149
                     /dbxref="REBASE:XbaI"
     Promoter        3206..3474
                     /gene="SV40 prom"
     Rep_Origin      3373..3450
                     /gene="SV40 origin"
     ORF             3440..4108
                     /sequence="ORF_2 rf(2)"
     misc_binding    3453..3465
                     /dbxref="REBASE:SfiI"
     Marker          3509..4108
                     /gene="puro marker"
     Other Gene      4718..4770
                     /gene="delta_U3 other"
     Other Gene      4771..4951
                     /gene="HIV-1_5_LTR other"
     Rep_Origin      5458..6077
                     /gene="pBR322 origin"
     Promoter        6335..6384
                     /gene="NEOKAN prom"
     misc_binding    6442..6447
                     /dbxref="REBASE:BclI"
     ORF             6473..7267
                     /sequence="ORF_3 rf(2)"
     Marker          6476..7264
                     /gene="NTP_II marker"
     Rep_Origin      7372..7678
                     /gene="f1 origin"
     Other Gene      7733..7834
                     /gene="RITR other"
     Other Gene      7733..7834
                     /gene="LITR other"
     Other Gene      7914..8062
                     /gene="Encap other"
BASE COUNT    1974 a   2052 c   2371 g   1914 t    0 others
ORIGIN
        1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg     
       61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg     
      121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct     
      181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt     
      241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga     
      301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg     
      361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga     
      421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa     
      481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa     
      541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag     
      601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat     
      661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga     
      721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta     
      781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat     
      841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     
      901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     
      961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     
     1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     
     1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     
     1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     
     1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct     
     1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     
     1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     
     1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     
     1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     
     1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     
     1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     
     1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt     
     1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt     
     1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca     
     1801 aaattttcgg gtttttcgaa cctagggagt gggaattggc tccggtgccc gtcagtgggc     
     1861 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg     
     1921 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct     
     1981 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt     
     2041 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg     
     2101 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccactggct gcagtacgtg     
     2161 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa     
     2221 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg     
     2281 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa     
     2341 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc     
     2401 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg     
     2461 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg     
     2521 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc     
     2581 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg     
     2641 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg     
     2701 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac     
     2761 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg     
     2821 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg     
     2881 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt     
     2941 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca     
     3001 tttcaggtgt cgtgaggaat ttcgactgct agctggtacc tgaattgagt acttacgtag     
     3061 gtaccccagt gtggtggcct gcaggtgaat tcactagtac cggtaggcct gtcgacgata     
     3121 tcgggcccgc ggccgctgga tcctctagac tgcagctcga gtacccatac gacgtcccag     
     3181 actacgcttg agtttaaaca cgcgtggtgt ggaaagtccc caggctcccc agcaggcaga     
     3241 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc     
     3301 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc     
     3361 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc     
     3421 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag     
     3481 aagtagtgag gaggcttttt tggaggccat gaccgagtac aagcccacgg tgcgcctcgc     
     3541 cacccgcgac gacgtccctc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc     
     3601 cgccacgcgc cacaccgtgg acccggaccg ccacatcgag cgggtcaccg agctgcaaga     
     3661 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc     
     3721 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat     
     3781 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg     
     3841 gctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc     
     3901 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc     
     3961 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta     
     4021 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg     
     4081 gtgcatgacc cgcaagcccg gtgcctgaac gcgttccgga aatcaacctc tggattacaa     
     4141 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata     
     4201 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc     
     4261 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg     
     4321 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac     
     4381 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat     
     4441 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt     
     4501 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat     
     4561 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc     
     4621 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc gccttcgccc tcagacgagt     
     4681 cggatctccc tttgggccgc ctccccgcct gtccggatgg aagggctaat tcactcccaa     
     4741 cgaatacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc     
     4801 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga     
     4861 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga     
     4921 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag     
     4981 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct     
     5041 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca     
     5101 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggcatcta     
     5161 tgtcgggtgc ggagaaagag gtaatgaaat ggcattatgg gtattatggg tctgcattaa     
     5221 tgaatcggcc aacggatgtc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg     
     5281 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg     
     5341 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa     
     5401 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc     
     5461 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc     
     5521 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag     
     5581 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct     
     5641 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta     
     5701 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc     
     5761 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc     
     5821 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt     
     5881 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct     
     5941 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc     
     6001 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca     
     6061 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta     
     6121 agggattttg gccatgagat tatcaaaaag gatcttcacc tagatccttt tcacgtagaa     
     6181 agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac     
     6241 aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata     
     6301 gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc     
     6361 tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg     
     6421 atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc gcatgattga     
     6481 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga     
     6541 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg     
     6601 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga     
     6661 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt     
     6721 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct     
     6781 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct     
     6841 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg     
     6901 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca     
     6961 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga     
     7021 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt     
     7081 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt     
     7141 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct     
     7201 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt     
     7261 cttctgaatt ttgttaaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa     
     7321 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag     
     7381 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg     
     7441 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga     
     7501 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg     
     7561 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg     
     7621 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc     
     7681 cgctacaggg cgcgtccatt cgccattcag gatcgaatta attcttaatt aacatcatca     
     7741 ataatatacc ttttttggat tgaagccaat atgataatga gggggtggag tttgtgacgt     
     7801 ggcgcggggc gtgggaacgg ggcgggtgac gtagtagtgt ggcggaagtg tgatgttgca     
     7861 agtgtggcgg aacacatgta agcgacggat gtggcaaaag tgacgttttt ggtgtgcgcc     
     7921 ggtgtacaca ggaagtgaca attttcgcgc ggttttaggc ggatgttgta gtaaatttgg     
     7981 gcgtaaccga gtaagatttg gccattttcg cgggaaaact gaataagagg aagtgaaatc     
     8041 tgaataattt tgtgttactc atagcgcgta atactctcat gagcggatac atatttgaat     
     8101 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg     
     8161 acgtaactat aacggtccta aggtagcgaa aatgtagtct tatgcaatac tcttgtagtc     
     8221 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc     
     8281 atgccgattg gtggaagtaa ggtggtacga t                                    
//