pLenti-III-HPV-16-E6-E7-No Selection

                            LOCUS       dna                      8052 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    457..462
                     /dbxref="REBASE:HpaI"
     misc_binding    457..462
                     /dbxref="REBASE:HincII"
     Other Gene      715..895
                     /gene="HIV-1_5_LTR other"
     Other Gene      1006..1050
                     /gene="HIV-1_psi_pack other"
     misc_binding    1092..1097
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  1557..1790
                     /gene="RRE reg"
     ORF             1600..2301
                     /sequence="ORF_1 rf(1)"
     Promoter        2885..2966
                     /gene="CMV prom"
     Regulatory_Seq  3022..3061
                     /gene="tetO reg"
     misc_binding    3068..3073
                     /dbxref="REBASE:XmaI"
     misc_binding    3068..3073
                     /dbxref="REBASE:SmaI"
     misc_binding    3074..3079
                     /dbxref="REBASE:ClaI"
     ORF             3929..4636
                     /sequence="ORF_2 rf(2)"
     misc_binding    3989..3994
                     /dbxref="REBASE:ApaI"
     misc_binding    3995..4002
                     /dbxref="REBASE:NotI"
     misc_binding    4010..4015
                     /dbxref="REBASE:XbaI"
     misc_binding    4022..4027
                     /dbxref="REBASE:XhoI"
     Other Gene      4625..4677
                     /gene="delta_U3 other"
     Other Gene      4678..4858
                     /gene="HIV-1_5_LTR other"
     Rep_Origin      5364..5983
                     /gene="pBR322 origin"
     Promoter        6636..6685
                     /gene="NEOKAN prom"
     misc_binding    6743..6748
                     /dbxref="REBASE:BclI"
     ORF             6774..7568
                     /sequence="ORF_3 rf(3)"
     Marker          6777..7565
                     /gene="NTP_II marker"
     Rep_Origin      7673..7978
                     /gene="f1 origin"
BASE COUNT    2158 a   1817 c   2104 g   1973 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta     
      481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag     
      541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg     
      601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact     
      661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct     
      721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt     
      781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac     
      841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc     
      901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc     
      961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa     
     1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg     
     1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata     
     1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc     
     1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga     
     1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc     
     1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca     
     1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag     
     1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta     
     1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga     
     1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg     
     1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg     
     1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc     
     1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg     
     1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat     
     1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac     
     1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat     
     1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca     
     2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga     
     2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg     
     2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa     
     2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga     
     2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag     
     2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa     
     2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat     
     2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt     
     2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa     
     2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc     
     2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct     
     2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt     
     2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg     
     2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca     
     2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca     
     2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc     
     3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag     
     3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accccagtgt ggtggcctgc     
     3121 aggtgaattc actagaggat ccttttatgc accaaaagag aactgcaatg tttcaggacc     
     3181 cacaggagcg acccagaaag ttaccacagt tatgcacaga gctgcaaaca actatacatg     
     3241 atataatatt agaatgtgtg tactgcaagc aacagttact gcgacgtgag gtatatgact     
     3301 ttgcttttcg ggatttatgc atagtatata gagatgggaa tccatatgct gtatgtgata     
     3361 aatgtttaaa gttttattct aaaattagtg agtatagaca ttattgttat agtttgtatg     
     3421 gaacaacatt agaacagcaa tacaacaaac cgttgtgtga tttgttaatt aggtgtatta     
     3481 actgtcaaaa gccactgtgt cctgaagaaa agcaaagaca tctggacaaa aagcaaagat     
     3541 tccataatat aaggggtcgg tggaccggtc gatgtatgtc ttgttgcaga tcatcaagaa     
     3601 cacgtagaga aacccagctg taatcatgca tggagataca cctacattgc atgaatatat     
     3661 gttagatttg caaccagaga caactgatct ctactgttat gagcaattaa atgacagctc     
     3721 agaggaggag gatgaaatag atggtccagc tggacaagca gaaccggaca gagcccatta     
     3781 caatattgta accttttgtt gcaagtgtga ctctacgctt cggttgtgcg tacaaagcac     
     3841 acacgtagac attcgtactt tggaagacct gttaatgggc acactaggaa ttgtgtgccc     
     3901 catctgttct cagaaaccat aatctaccat ggctgatcct gcaggtaccg gatccggtac     
     3961 cgagctcgaa ttcaagctta agtttatcgg gcccgcggcc gctggatcct ctagactgca     
     4021 gctcgagaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta     
     4081 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc     
     4141 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga     
     4201 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac     
     4261 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc     
     4321 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc     
     4381 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg     
     4441 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc     
     4501 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc     
     4561 gcgtctcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgtc     
     4621 cggatggaag ggctaattca ctcccaacga atacaagatc tgctttttgc ttgtactggg     
     4681 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg     
     4741 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt     
     4801 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt     
     4861 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag     
     4921 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca     
     4981 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc     
     5041 aatgtatctt atcatgtctg gcatctatgt cgggtgcgga gaaagaggta atgaaatggc     
     5101 attatgggta ttatgggtct gcattaatga atcggccaac gatcccggtg tgaaataccg     
     5161 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac     
     5221 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata     
     5281 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa     
     5341 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct     
     5401 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa     
     5461 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg     
     5521 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca     
     5581 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa     
     5641 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg     
     5701 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg     
     5761 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg     
     5821 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc     
     5881 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag     
     5941 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac     
     6001 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc     
     6061 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag     
     6121 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt     
     6181 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag     
     6241 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca     
     6301 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact     
     6361 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca     
     6421 gttaatagtt tgcgcaacgt tgttgaaaaa ggatcttcac ctagatcctt ttcacgtaga     
     6481 aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga     
     6541 caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat     
     6601 agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct     
     6661 ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttctcgccg ccaaggatct     
     6721 gatggcgcag gggatcaagc tctgatcaag agacaggatg aggatcgttt cgcatgattg     
     6781 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg     
     6841 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg     
     6901 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg     
     6961 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg     
     7021 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc     
     7081 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc     
     7141 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc     
     7201 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc     
     7261 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg     
     7321 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct     
     7381 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt     
     7441 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc     
     7501 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt     
     7561 tcttctgaat tttgttaaaa tttttgttaa atcagctcat tttttaacca ataggccgaa     
     7621 atcggcaaca tcccttataa atcaaaagaa tagaccgcga tagggttgag tgttgttcca     
     7681 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc     
     7741 gtctatcagg gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttgcggtcg     
     7801 aggtgccgta aagctctaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg     
     7861 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg     
     7921 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgcgcg cttaatgcgc     
     7981 cgctacaggg cgcgtccatt cgccattcag gatcgaatta attcttaatt aacatcatca     
     8041 ataatatacc tt                                                         
//