pLenti-III-PGK

                            LOCUS       dna                      7609 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      109..289
                     /gene="HIV-1_5_LTR other"
     Other Gene      400..444
                     /gene="HIV-1_psi_pack other"
     misc_binding    486..491
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  951..1184
                     /gene="RRE reg"
     ORF             994..1695
                     /sequence="ORF_1 rf(1)"
     misc_binding    2358..2363
                     /dbxref="REBASE:KpnI"
     misc_binding    2385..2390
                     /dbxref="REBASE:EcoRI"
     misc_binding    2409..2414
                     /dbxref="REBASE:HincII"
     misc_binding    2415..2420
                     /dbxref="REBASE:EcoRV"
     misc_binding    2421..2426
                     /dbxref="REBASE:ApaI"
     misc_binding    2427..2434
                     /dbxref="REBASE:NotI"
     misc_binding    2436..2441
                     /dbxref="REBASE:BamHI"
     misc_binding    2442..2447
                     /dbxref="REBASE:XbaI"
     misc_binding    2454..2459
                     /dbxref="REBASE:XhoI"
     Promoter        2504..2772
                     /gene="SV40 prom"
     Rep_Origin      2671..2748
                     /gene="SV40 origin"
     ORF             2738..3406
                     /sequence="ORF_2 rf(2)"
     misc_binding    2751..2763
                     /dbxref="REBASE:SfiI"
     Marker          2807..3406
                     /gene="puro marker"
     Other Gene      4016..4068
                     /gene="delta_U3 other"
     Other Gene      4069..4249
                     /gene="HIV-1_5_LTR other"
     Rep_Origin      4756..5375
                     /gene="pBR322 origin"
     Promoter        5633..5682
                     /gene="NEOKAN prom"
     misc_binding    5740..5745
                     /dbxref="REBASE:BclI"
     ORF             5771..6565
                     /sequence="ORF_3 rf(2)"
     Marker          5774..6562
                     /gene="NTP_II marker"
     Rep_Origin      6670..6976
                     /gene="f1 origin"
     Other Gene      7031..7132
                     /gene="RITR other"
     Other Gene      7031..7132
                     /gene="LITR other"
     Other Gene      7212..7360
                     /gene="Encap other"
BASE COUNT    1857 a   1909 c   2124 g   1719 t    0 others
ORIGIN
        1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg     
       61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg     
      121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct     
      181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt     
      241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga     
      301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg     
      361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga     
      421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa     
      481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa     
      541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag     
      601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat     
      661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga     
      721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta     
      781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat     
      841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     
      901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     
      961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     
     1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     
     1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     
     1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     
     1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct     
     1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     
     1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     
     1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     
     1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     
     1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     
     1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     
     1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt     
     1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt     
     1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca     
     1801 aaattttcgg gtttttcgaa cctagaaatt ctaccgggta ggggaggcgc ttttcccaag     
     1861 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct     
     1921 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg     
     1981 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct     
     2041 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga     
     2101 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg     
     2161 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg     
     2221 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct     
     2281 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctc     
     2341 aattgagtac ttacgtaggt accccagtgt ggtggcctgc aggtgaattc actagtaccg     
     2401 gtaggcctgt cgacgatatc gggcccgcgg ccgctggatc ctctagactg cagctcgagt     
     2461 acccatacga cgtcccagac tacgcttgag tttaaacacg cgtggtgtgg aaagtcccca     
     2521 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt     
     2581 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca     
     2641 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc     
     2701 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg     
     2761 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggccatga ccgagtacaa     
     2821 gcccacggtg cgcctcgcca cccgcgacga cgtccctcgg gccgtacgca ccctcgccgc     
     2881 cgcgttcgcc gactaccccg ccacgcgcca caccgtggac ccggaccgcc acatcgagcg     
     2941 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg     
     3001 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg     
     3061 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc     
     3121 gcagcaacag atggaagggc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct     
     3181 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct     
     3241 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc     
     3301 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc     
     3361 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaacgc gttccggaaa     
     3421 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc     
     3481 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat     
     3541 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg     
     3601 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg     
     3661 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat     
     3721 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt     
     3781 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc     
     3841 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa     
     3901 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctcgc     
     3961 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgt ccggatggaa     
     4021 gggctaattc actcccaacg aatacaagat ctgctttttg cttgtactgg gtctctctgg     
     4081 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct     
     4141 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt     
     4201 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca     
     4261 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg     
     4321 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca     
     4381 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct     
     4441 tatcatgtct ggcatctatg tcgggtgcgg agaaagaggt aatgaaatgg cattatgggt     
     4501 attatgggtc tgcattaatg aatcggccaa cggatgtcgg tgtgaaatac cgcacagatg     
     4561 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg     
     4621 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc     
     4681 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag     
     4741 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca     
     4801 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca     
     4861 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg     
     4921 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag     
     4981 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt     
     5041 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca     
     5101 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg     
     5161 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt     
     5221 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc     
     5281 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg     
     5341 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg     
     5401 gaacgaaaac tcacgttaag ggattttggc catgagatta tcaaaaagga tcttcaccta     
     5461 gatccttttc acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag     
     5521 ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag     
     5581 tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt     
     5641 gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt     
     5701 cttgccgcca aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg     
     5761 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga     
     5821 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt     
     5881 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct     
     5941 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg     
     6001 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt     
     6061 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc     
     6121 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc     
     6181 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga     
     6241 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag     
     6301 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat     
     6361 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg     
     6421 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc     
     6481 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta     
     6541 tcgccttctt gacgagttct tctgaatttt gttaaaattt ttgttaaatc agctcatttt     
     6601 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag     
     6661 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg     
     6721 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat     
     6781 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc     
     6841 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga     
     6901 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac     
     6961 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcagga tcgaattaat     
     7021 tcttaattaa catcatcaat aatatacctt ttttggattg aagccaatat gataatgagg     
     7081 gggtggagtt tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg     
     7141 cggaagtgtg atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg     
     7201 acgtttttgg tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg     
     7261 atgttgtagt aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga     
     7321 ataagaggaa gtgaaatctg aataattttg tgttactcat agcgcgtaat actctcatga     
     7381 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     
     7441 cccgaaaagt gccacctgac gtaactataa cggtcctaag gtagcgaaaa tgtagtctta     
     7501 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg     
     7561 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgat                 
//