pLenti-III-UBC
Vector Name | pLenti-III-UBC |
VectorType | Lentiviral vector |
Antibiotic Information | Puromycin |
Sequencing Primers | UBC Sequencing Primer 5'---TGAAGCTCCGGTTTTGAACT ---3' SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
Additional Information | No Additional Information |
Double-click on the features for more information
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagaggcc tccgcgccgg gttttggcgc ctcccgcggg 1861 cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga 1921 tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc 1981 ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt 2041 ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg 2101 atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct 2161 agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca 2221 cttggtgagt agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt 2281 gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg 2341 ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg 2401 gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg 2461 gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat 2521 gggctggggc accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt 2581 ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct 2641 ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag 2701 ggtggggcca cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt 2761 cgggcctagg gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat 2821 aagtgaggcg tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc 2881 tccggttttg aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac 2941 cttttgaaat gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt 3001 ccgctaaatt ctggccgttt ttggcttttt tgttagacgc tagcggatca attgagtact 3061 tacgtaggta ccccagtgtg gtggcctgca ggtgaattca ctagtaccgg taggcctgtc 3121 gacgatatcg ggcccgcggc cgctggatcc tctagactgc agctcgagta cccatacgac 3181 gtcccagact acgcttgagt ttaaacacgc gtggtgtgga aagtccccag gctccccagc 3241 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 3301 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 3361 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 3421 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 3481 attccagaag tagtgaggag gcttttttgg aggccatgac cgagtacaag cccacggtgc 3541 gcctcgccac ccgcgacgac gtccctcggg ccgtacgcac cctcgccgcc gcgttcgccg 3601 actaccccgc cacgcgccac accgtggacc cggaccgcca catcgagcgg gtcaccgagc 3661 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg 3721 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg 3781 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga 3841 tggaagggct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg 3901 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg 3961 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc 4021 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc 4081 gcacctggtg catgacccgc aagcccggtg cctgaacgcg ttccggaaat caacctctgg 4141 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat 4201 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt 4261 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca 4321 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg 4381 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg 4441 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca 4501 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca 4561 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc 4621 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctcgcc ttcgccctca 4681 gacgagtcgg atctcccttt gggccgcctc cccgcctgtc cggatggaag ggctaattca 4741 ctcccaacga atacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat 4801 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 4861 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 4921 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat 4981 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat 5041 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 5101 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 5161 gcatctatgt cgggtgcgga gaaagaggta atgaaatggc attatgggta ttatgggtct 5221 gcattaatga atcggccaac ggatgtcggt gtgaaatacc gcacagatgc gtaaggagaa 5281 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 5341 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 5401 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 5461 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 5521 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 5581 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 5641 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 5701 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 5761 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 5821 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 5881 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 5941 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 6001 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 6061 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 6121 cacgttaagg gattttggcc atgagattat caaaaaggat cttcacctag atccttttca 6181 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta 6241 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat 6301 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg 6361 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa 6421 ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca 6481 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 6541 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 6601 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 6661 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 6721 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 6781 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 6841 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 6901 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 6961 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg 7021 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 7081 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 7141 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 7201 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 7261 acgagttctt ctgaattttg ttaaaatttt tgttaaatca gctcattttt taaccaatag 7321 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 7381 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 7441 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 7501 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 7561 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 7621 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 7681 aatgcgccgc tacagggcgc gtccattcgc cattcaggat cgaattaatt cttaattaac 7741 atcatcaata atataccttt tttggattga agccaatatg ataatgaggg ggtggagttt 7801 gtgacgtggc gcggggcgtg ggaacggggc gggtgacgta gtagtgtggc ggaagtgtga 7861 tgttgcaagt gtggcggaac acatgtaagc gacggatgtg gcaaaagtga cgtttttggt 7921 gtgcgccggt gtacacagga agtgacaatt ttcgcgcggt tttaggcgga tgttgtagta 7981 aatttgggcg taaccgagta agatttggcc attttcgcgg gaaaactgaa taagaggaag 8041 tgaaatctga ataattttgt gttactcata gcgcgtaata ctctcatgag cggatacata 8101 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 8161 ccacctgacg taactataac ggtcctaagg tagcgaaaat gtagtcttat gcaatactct 8221 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc 8281 accgtgcatg ccgattggtg gaagtaaggt ggtacgat //
LOCUS dna 8318 bp FEATURES Location/Qualifiers Other Gene 109..289 /gene="HIV-1_5_LTR other" Other Gene 400..444 /gene="HIV-1_psi_pack other" misc_binding 486..491 /dbxref="REBASE:NruI" Regulatory_Seq 951..1184 /gene="RRE reg" ORF 994..1695 /sequence="ORF_1 rf(1)" Promoter 1827..3038 /gene="hUbC prom" misc_binding 3067..3072 /dbxref="REBASE:KpnI" misc_binding 3094..3099 /dbxref="REBASE:EcoRI" misc_binding 3118..3123 /dbxref="REBASE:HincII" misc_binding 3124..3129 /dbxref="REBASE:EcoRV" misc_binding 3130..3135 /dbxref="REBASE:ApaI" misc_binding 3136..3143 /dbxref="REBASE:NotI" misc_binding 3145..3150 /dbxref="REBASE:BamHI" misc_binding 3151..3156 /dbxref="REBASE:XbaI" misc_binding 3163..3168 /dbxref="REBASE:XhoI" Promoter 3213..3481 /gene="SV40 prom" Rep_Origin 3380..3457 /gene="SV40 origin" ORF 3447..4115 /sequence="ORF_2 rf(3)" misc_binding 3460..3472 /dbxref="REBASE:SfiI" Marker 3516..4115 /gene="puro marker" Other Gene 4725..4777 /gene="delta_U3 other" Other Gene 4778..4958 /gene="HIV-1_5_LTR other" Rep_Origin 5465..6084 /gene="pBR322 origin" Promoter 6342..6391 /gene="NEOKAN prom" misc_binding 6449..6454 /dbxref="REBASE:BclI" ORF 6480..7274 /sequence="ORF_3 rf(3)" Marker 6483..7271 /gene="NTP_II marker" Rep_Origin 7379..7685 /gene="f1 origin" Other Gene 7740..7841 /gene="RITR other" Other Gene 7740..7841 /gene="LITR other" Other Gene 7921..8069 /gene="Encap other" BASE COUNT 1980 a 2027 c 2394 g 1917 t 0 others ORIGIN 1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagaggcc tccgcgccgg gttttggcgc ctcccgcggg 1861 cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga 1921 tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc 1981 ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt 2041 ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg 2101 atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct 2161 agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca 2221 cttggtgagt agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt 2281 gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg 2341 ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg 2401 gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg 2461 gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat 2521 gggctggggc accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt 2581 ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct 2641 ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag 2701 ggtggggcca cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt 2761 cgggcctagg gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat 2821 aagtgaggcg tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc 2881 tccggttttg aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac 2941 cttttgaaat gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt 3001 ccgctaaatt ctggccgttt ttggcttttt tgttagacgc tagcggatca attgagtact 3061 tacgtaggta ccccagtgtg gtggcctgca ggtgaattca ctagtaccgg taggcctgtc 3121 gacgatatcg ggcccgcggc cgctggatcc tctagactgc agctcgagta cccatacgac 3181 gtcccagact acgcttgagt ttaaacacgc gtggtgtgga aagtccccag gctccccagc 3241 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 3301 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 3361 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 3421 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 3481 attccagaag tagtgaggag gcttttttgg aggccatgac cgagtacaag cccacggtgc 3541 gcctcgccac ccgcgacgac gtccctcggg ccgtacgcac cctcgccgcc gcgttcgccg 3601 actaccccgc cacgcgccac accgtggacc cggaccgcca catcgagcgg gtcaccgagc 3661 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg 3721 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg 3781 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga 3841 tggaagggct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg 3901 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg 3961 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc 4021 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc 4081 gcacctggtg catgacccgc aagcccggtg cctgaacgcg ttccggaaat caacctctgg 4141 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat 4201 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt 4261 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca 4321 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg 4381 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg 4441 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca 4501 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca 4561 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc 4621 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctcgcc ttcgccctca 4681 gacgagtcgg atctcccttt gggccgcctc cccgcctgtc cggatggaag ggctaattca 4741 ctcccaacga atacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat 4801 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 4861 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 4921 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat 4981 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat 5041 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 5101 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 5161 gcatctatgt cgggtgcgga gaaagaggta atgaaatggc attatgggta ttatgggtct 5221 gcattaatga atcggccaac ggatgtcggt gtgaaatacc gcacagatgc gtaaggagaa 5281 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 5341 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 5401 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 5461 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 5521 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 5581 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 5641 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 5701 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 5761 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 5821 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 5881 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 5941 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 6001 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 6061 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 6121 cacgttaagg gattttggcc atgagattat caaaaaggat cttcacctag atccttttca 6181 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta 6241 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat 6301 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg 6361 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa 6421 ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca 6481 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 6541 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 6601 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 6661 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 6721 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 6781 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 6841 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 6901 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 6961 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg 7021 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 7081 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 7141 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 7201 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 7261 acgagttctt ctgaattttg ttaaaatttt tgttaaatca gctcattttt taaccaatag 7321 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 7381 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 7441 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 7501 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 7561 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 7621 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 7681 aatgcgccgc tacagggcgc gtccattcgc cattcaggat cgaattaatt cttaattaac 7741 atcatcaata atataccttt tttggattga agccaatatg ataatgaggg ggtggagttt 7801 gtgacgtggc gcggggcgtg ggaacggggc gggtgacgta gtagtgtggc ggaagtgtga 7861 tgttgcaagt gtggcggaac acatgtaagc gacggatgtg gcaaaagtga cgtttttggt 7921 gtgcgccggt gtacacagga agtgacaatt ttcgcgcggt tttaggcgga tgttgtagta 7981 aatttgggcg taaccgagta agatttggcc attttcgcgg gaaaactgaa taagaggaag 8041 tgaaatctga ataattttgt gttactcata gcgcgtaata ctctcatgag cggatacata 8101 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 8161 ccacctgacg taactataac ggtcctaagg tagcgaaaat gtagtcttat gcaatactct 8221 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc 8281 accgtgcatg ccgattggtg gaagtaaggt ggtacgat //