pLenti-III-mir-GFP-Cloning
| Vector Name | pLenti-III-mir-GFP-Cloning |
| VectorType | Lentiviral miRNA Vector |
| Sequencing Primers | EGFP-C Sequencing Primer: 5'--CAT GGT CCT GCT GGA GTT CGT G--3' Reverse Sequencing Primer: 5'--GAC TTT CCA CAC CTG GTT GCT GAC --3' |
| Additional Information | No Additional Information |
Double-click on the features for more information
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagaatc tagaggtacc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
3121 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
3181 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
3241 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
3301 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
3361 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
3421 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
3481 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
3541 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
3601 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
3661 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
3721 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
3781 tcggcatgga cgagctgtac aagtgaggta ccgatatcga attcatagct agccctgcag
3841 gtctagactc gaggcggccg cagtcgagta cccatacgac gtcccagact acgcttgagt
3901 ttaaacacgc gtggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
3961 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
4021 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
4081 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
4141 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
4201 gcttttttgg aggccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
4261 gtccctcggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
4321 accgtggacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
4381 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
4441 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
4501 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaagggct cctggcgccg
4561 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
4621 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
4681 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
4741 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
4801 aagcccggtg cctgaacgcg ttccggaaat caacctctgg attacaaaat ttgtgaaaga
4861 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
4921 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
4981 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
5041 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
5101 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
5161 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
5221 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
5281 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
5341 ccggctctgc ggcctcttcc gcgtctcgcc ttcgccctca gacgagtcgg atctcccttt
5401 gggccgcctc cccgcctgtc cggatggaag ggctaattca ctcccaacga atacaagatc
5461 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
5521 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
5581 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
5641 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
5701 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
5761 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
5821 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gcatctatgt cgggtgcgga
5881 gaaagaggta atgaaatggc attatgggta ttatgggtct gcattaatga atcggccaac
5941 gatcccggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
6001 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
6061 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
6121 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
6181 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
6241 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
6301 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
6361 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
6421 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
6481 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
6541 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
6601 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
6661 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
6721 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
6781 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
6841 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
6901 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
6961 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
7021 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
7081 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
7141 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
7201 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgaaaaa ggatcttcac
7261 ctagatcctt ttcacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt
7321 cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg
7381 cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga
7441 attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc
7501 tttctcgccg ccaaggatct gatggcgcag gggatcaagc tctgatcaag agacaggatg
7561 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
7621 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
7681 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
7741 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
7801 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
7861 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
7921 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
7981 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
8041 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
8101 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
8161 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
8221 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
8281 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
8341 ctatcgcctt cttgacgagt tcttctgaat tttgttaaaa tttttgttaa atcagctcat
8401 tttttaacca ataggccgaa atcggcaaca tcccttataa atcaaaagaa tagaccgcga
8461 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
8521 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccca
8581 aatcaagttt tttgcggtcg aggtgccgta aagctctaaa tcggaaccct aaagggagcc
8641 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
8701 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
8761 cacccgcgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gatcgaatta
8821 attcttaatt aacatcatca ataatatacc tt
LOCUS dna 8852 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
ORF 2901..3806
/sequence="ORF_4 rf(3)"
Regulatory_Seq 3022..3061
/gene="tetO reg"
Reporter 3087..3803
/gene="EGFP reporter"
misc_binding 3813..3818
/dbxref="REBASE:EcoRV"
misc_binding 3819..3824
/dbxref="REBASE:EcoRI"
misc_binding 3835..3840
/dbxref="REBASE:PstI"
misc_binding 3848..3853
/dbxref="REBASE:XhoI"
misc_binding 3854..3861
/dbxref="REBASE:NotI"
Promoter 3913..4181
/gene="SV40 prom"
Rep_Origin 4080..4157
/gene="SV40 origin"
ORF 4147..4815
/sequence="ORF_2 rf(1)"
misc_binding 4160..4172
/dbxref="REBASE:SfiI"
Marker 4216..4815
/gene="puro marker"
Other Gene 5425..5477
/gene="delta_U3 other"
Other Gene 5478..5658
/gene="HIV-1_5_LTR other"
Rep_Origin 6164..6783
/gene="pBR322 origin"
Promoter 7436..7485
/gene="NEOKAN prom"
misc_binding 7543..7548
/dbxref="REBASE:BclI"
ORF 7574..8368
/sequence="ORF_3 rf(2)"
Marker 7577..8365
/gene="NTP_II marker"
Rep_Origin 8473..8778
/gene="f1 origin"