pLenti-SFFV-dCas9-VPR

                            
FEATURES             Location/Qualifiers
     source          1..13669
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             715..895
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    942..1067
                     /note="HIV-1 psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1557..1790
                     /note="HIV-1 RRE"
                     /note="The Rev response element (RRE) of human 
                     immunodeficiency virus type 1 allows for Rev-dependent mRNA
                     export from the nucleus to the cytoplasm."
                     /note="color: #ffe4c4"
     CDS             5471..5482
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /note="color: #cc99b2
                     Cleavage site after base 5482"
                     /translation="IEGR"
     CDS             7000..7020
                     /codon_start=1
                     /locus_tag="
                     "
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /note="
                     "
                     /note="color: #993366"
                     /protein_id="
                     "
                     /translation="PKKKRKV"
     protein_bind    7057..7081
                     /gene="mutant version of lambda attB"
                     /bound_moiety="BP Clonase"
                     /note="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b"
     CDS             7285..7305
                     /codon_start=1
                     /locus_tag="
                     "
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /note="
                     "
                     /note="color: #993366"
                     /protein_id="
                     "
                     /translation="PKKKRKV"
     CDS             8686..8706
                     /codon_start=1
                     /locus_tag="
                     "
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /note="
                     "
                     /note="color: #993366"
                     /protein_id="
                     "
                     /translation="PKKKRKV"
     rep_origin      8891..9026
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             9027..9626
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    9645..10232
                     /note="WPRE"
                     /note="woodchuck posttranscriptional regulatory element"
                     /note="color: #ffffff"
     CDS             complement(10116..10127)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /note="color: #cc99b2
                     Cleavage site after base 10115"
                     /translation="IEGR"
     misc_feature    10242..10475
                     /note="3'LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             10295..10475
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    10547..10668
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(10996..11584)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             12391..13185
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
      481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
      541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
     1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
     2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
     2401 aattcaaaat tttcgggttt ttcgaaccta ggtgcagtaa cgccattttg caaggcatgg
     2461 aaaaatacca aaccaagaat agagaagttc agatcaaggg cgggtacatg aaaatagcta
     2521 acgttgggcc aaacaggata tctgcggtga gcagtttcgg ccccggcccg gggccaagaa
     2581 cagatggtca ccgcagtttc ggccccggcc cgaggccaag aacagatggt ccccagatat
     2641 ggcccaaccc tcagcagttt cttaagaccc atcagatgtt tccaggctcc cccaaggacc
     2701 tgaaatgacc ctgcgcctta tttgaattaa ccaatcagcc tgcttctcgc ttctgttcgc
     2761 gcgcttctgc ttcccgagct ctataaaaga gctcacaacc cctcactcgg cgcgccagtc
     2821 ctccgacaga ctgagtcgcc cggctagcaa taattttgtt taactttaag aaggagatat
     2881 accatggaca agaagtactc cattgggctc gcgatcggca caaacagcgt cggctgggcc
     2941 gtcattacgg acgagtacaa ggtgccgagc aaaaaattca aagttctggg caataccgat
     3001 cgccacagca taaagaagaa cctcattggc gccctcctgt tcgactccgg ggagacggcc
     3061 gaagccacgc ggctcaaaag aacagcacgg cgcagatata cccgcagaaa gaatcggatc
     3121 tgctacctgc aggagatctt tagtaatgag atggctaagg tggatgactc tttcttccat
     3181 aggctggagg agtccttttt ggtggaggag gataaaaagc acgagcgcca cccaatcttt
     3241 ggcaatatcg tggacgaggt ggcgtaccat gaaaagtacc caaccatata tcatctgagg
     3301 aagaagcttg tagacagtac tgataaggct gacttgcggt tgatctatct cgcgctggcg
     3361 catatgatca aatttcgggg acacttcctc atcgaggggg acctgaaccc agacaacagc
     3421 gatgtcgaca aactctttat ccaactggtt cagacttaca atcagctttt cgaagagaac
     3481 ccgatcaacg catccggagt tgacgccaaa gcaatcctga gcgctaggct gtccaaatcc
     3541 cggcggctcg aaaacctcat cgcacagctc cctggggaga agaagaacgg cctgtttggt
     3601 aatcttatcg ccctgtcact cgggctgacc cccaacttta aatctaactt cgacctggcc
     3661 gaagatgcca agcttcaact gagcaaagac acctacgatg atgatctcga caatctgctg
     3721 gcccagatcg gcgaccagta cgcagacctt tttttggcgg caaagaacct gtcagacgcc
     3781 attctgctga gtgatattct gcgagtgaac acggagatca ccaaagctcc gctgagcgct
     3841 agtatgatca agcgctatga tgagcaccac caagacttga ctttgctgaa ggcccttgtc
     3901 agacagcaac tgcctgagaa gtacaaggaa attttcttcg atcagtctaa aaatggctac
     3961 gccggataca ttgacggcgg agcaagccag gaggaatttt acaaatttat taagcccatc
     4021 ttggaaaaaa tggacggcac cgaggagctg ctggtaaagc ttaacagaga agatctgttg
     4081 cgcaaacagc gcactttcga caatggaagc atcccccacc agattcacct gggcgaactg
     4141 cacgctatcc tcaggcggca agaggatttc tacccctttt tgaaagataa cagggaaaag
     4201 attgagaaaa tcctcacatt tcggataccc tactatgtag gccccctcgc ccggggaaat
     4261 tccagattcg cgtggatgac tcgcaaatca gaagagacca tcactccctg gaacttcgag
     4321 gaagtcgtgg ataagggggc ctctgcccag tccttcatcg aaaggatgac taactttgat
     4381 aaaaatctgc ctaacgaaaa ggtgcttcct aaacactctc tgctgtacga gtacttcaca
     4441 gtttataacg agctcaccaa ggtcaaatac gtcacagaag ggatgagaaa gccagcattc
     4501 ctgtctggag agcagaagaa agctatcgtg gacctcctct tcaagacgaa ccggaaagtt
     4561 accgtgaaac agctcaaaga agactatttc aaaaagattg aatgtttcga ctctgttgaa
     4621 atcagcggag tggaggatcg cttcaacgca tccctgggaa cgtatcacga tctcctgaaa
     4681 atcattaaag acaaggactt cctggacaat gaggagaacg aggacattct tgaggacatt
     4741 gtcctcaccc ttacgttgtt tgaagatagg gagatgattg aagaacgctt gaaaacttac
     4801 gctcatctct tcgacgacaa agtcatgaaa cagctcaaga ggcgccgata tacaggatgg
     4861 gggcggctgt caagaaaact gatcaatggg atccgagaca agcagagtgg aaagacaatc
     4921 ctggattttc ttaagtccga tggatttgcc aaccggaact tcatgcagtt gatccatgat
     4981 gactctctca cctttaagga ggacatccag aaagcacaag tttctggcca gggggacagt
     5041 cttcacgagc acatcgctaa tcttgcaggt agcccagcta tcaaaaaggg aatactgcag
     5101 accgttaagg tcgtggatga actcgtcaaa gtaatgggaa ggcataagcc cgagaatatc
     5161 gttatcgaga tggcccgaga gaaccaaact acccagaagg gacagaagaa cagtagggaa
     5221 aggatgaaga ggattgaaga gggtataaaa gaactggggt cccaaatcct taaggaacac
     5281 ccagttgaaa acacccagct tcagaatgag aagctctacc tgtactacct gcagaacggc
     5341 agggacatgt acgtggatca ggaactggac atcaatcggc tctccgacta cgacgtggat
     5401 gcgatcgtgc cccagtcttt tctcaaagat gattctattg ataataaagt gttgacaaga
     5461 tccgataaaa atagagggaa gagtgataac gtcccctcag aagaagttgt caagaaaatg
     5521 aaaaattatt ggcggcagct gctgaacgcc aaactgatca cacaacggaa gttcgataat
     5581 ctgactaagg ctgaacgagg tggcctgtct gagttggata aagccggctt catcaaaagg
     5641 cagcttgttg agacacgcca gatcaccaag cacgtggccc aaattctcga ttcacgcatg
     5701 aacaccaagt acgatgaaaa tgacaaactg attcgagagg tgaaagttat tactctgaag
     5761 tctaagctgg tctcagattt cagaaaggac tttcagtttt ataaggtgag agagatcaac
     5821 aattaccacc atgcgcatga tgcctacctg aatgcagtgg taggcactgc acttatcaaa
     5881 aaatatccca agcttgaatc tgaatttgtt tacggagact ataaagtgta cgatgttagg
     5941 aaaatgatcg caaagtctga gcaggaaata ggcaaggcca ccgctaagta cttcttttac
     6001 agcaatatta tgaatttttt caagaccgag attacactgg ccaatggaga gattcggaag
     6061 cgaccactta tcgaaacaaa cggagaaaca ggagaaatcg tgtgggacaa gggtagggat
     6121 ttcgcgacag tccggaaggt cctgtccatg ccgcaggtga acatcgttaa aaagaccgaa
     6181 gtacagaccg gaggcttctc caaggaaagt atcctcccga aaaggaacag cgacaagctg
     6241 atcgcacgca aaaaagattg ggaccccaag aaatacggcg gattcgattc tcctacagtc
     6301 gcttacagtg tactggttgt ggccaaagtg gagaaaggga agtctaaaaa actcaaaagc
     6361 gtcaaggaac tgctgggcat cacaatcatg gagcgatcaa gcttcgaaaa aaaccccatc
     6421 gactttctcg aggcgaaagg atataaagag gtcaaaaaag acctcatcat taagcttccc
     6481 aagtactctc tctttgagct tgaaaacggc cggaaacgaa tgctcgctag tgcgggcgag
     6541 ctgcagaaag gtaacgagct ggcactgccc tctaaatacg ttaatttctt gtatctggcc
     6601 agccactatg aaaagctcaa agggtctccc gaagataatg agcagaagca gctgttcgtg
     6661 gaacaacaca aacactacct tgatgagatc atcgagcaaa taagcgaatt ctccaaaaga
     6721 gtgatcctcg ccgacgctaa cctcgataag gtgctttctg cttacaataa gcacagggat
     6781 aagcccatca gggagcaggc agaaaacatt atccacttgt ttactctgac caacttgggc
     6841 gcgcctgcag ccttcaagta cttcgacacc accatagaca gaaagcggta cacctctaca
     6901 aaggaggtcc tggacgccac actgattcat cagtcaatta cggggctcta tgaaacaaga
     6961 atcgacctct ctcagctcgg tggagacagc agggctgacc ccaagaagaa gaggaaggtg
     7021 tcgccaggga tccgtcgact tgacgcgttg atatcaacaa gtttgtacaa aaaagcaggc
     7081 tacaaagagg ccagcggttc cggacgggct gacgcattgg acgattttga tctggatatg
     7141 ctgggaagtg acgccctcga tgattttgac cttgacatgc ttggttcgga tgcccttgat
     7201 gactttgacc tcgacatgct cggcagtgac gcccttgatg atttcgacct ggacatgctg
     7261 attaactcaa gaagttccgg atctccgaaa aagaaacgca aagttggtag ccagtacctg
     7321 cccgacaccg acgaccggca ccggatcgag gaaaagcgga agcggaccta cgagacattc
     7381 aagagcatca tgaagaagtc ccccttcagc ggccccaccg accctagacc tccacctaga
     7441 agaatcgccg tgcccagcag atccagcgcc agcgtgccaa aacctgcccc ccagccttac
     7501 cccttcacca gcagcctgag caccatcaac tacgacgagt tccctaccat ggtgttcccc
     7561 agcggccaga tctctcaggc ctctgctctg gctccagccc ctcctcaggt gctgcctcag
     7621 gctcctgctc ctgcaccagc tccagccatg gtgtctgcac tggctcaggc accagcaccc
     7681 gtgcctgtgc tggctcctgg acctccacag gctgtggctc caccagcccc taaacctaca
     7741 caggccggcg agggcacact gtctgaagct ctgctgcagc tgcagttcga cgacgaggat
     7801 ctgggagccc tgctgggaaa cagcaccgat cctgccgtgt tcaccgacct ggccagcgtg
     7861 gacaacagcg agttccaaca gctgctgaac cagggcatcc ctgtggcccc tcacaccacc
     7921 gagcccatgc tgatggaata ccccgaggcc atcacccggc tcgtgacagg cgctcagagg
     7981 cctcctgatc cagctcctgc ccctctggga gcaccaggcc tgcctaatgg actgctgtct
     8041 ggcgacgagg acttcagctc tatcgccgat atggatttct cagccttgct gggctctggc
     8101 agcggcagcc gggattccag ggaagggatg tttttgccga agcctgaggc cggctccgct
     8161 attagtgacg tgtttgaggg ccgcgaggtg tgccagccaa aacgaatccg gccatttcat
     8221 cctccaggaa gtccatgggc caaccgccca ctccccgcca gcctcgcacc aacaccaacc
     8281 ggtccagtac atgagccagt cgggtcactg accccggcac cagtccctca gccactggat
     8341 ccagcgcccg cagtgactcc cgaggccagt cacctgttgg aggatcccga tgaagagacg
     8401 agccaggctg tcaaagccct tcgggagatg gccgatactg tgattcccca gaaggaagag
     8461 gctgcaatct gtggccaaat ggacctttcc catccgcccc caaggggcca tctggatgag
     8521 ctgacaacca cacttgagtc catgaccgag gatctgaacc tggactcacc cctgaccccg
     8581 gaattgaacg agattctgga taccttcctg aacgacgagt gcctcttgca tgccatgcat
     8641 atcagcacag gactgtccat cttcgacaca tctctgttta ctagtccaaa aaagaaaaga
     8701 aaggtgtgag tttaaacacg cgtggtgtgg aaagtcccca ggctccccag caggcagaag
     8761 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     8821 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     8881 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     8941 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     9001 gtagtgagga ggcttttttg gaggccatga ccgagtacaa gcccacggtg cgcctcgcca
     9061 cccgcgacga cgtccctcgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     9121 ccacgcgcca caccgtggac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     9181 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     9241 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     9301 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaagggc
     9361 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     9421 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     9481 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     9541 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     9601 gcatgacccg caagcccggt gcctgaggta ccacgcgttc cggaaatcaa cctctggatt
     9661 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     9721 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     9781 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     9841 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     9901 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     9961 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
    10021 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
    10081 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
    10141 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctcgccttc gccctcagac
    10201 gagtcggatc tccctttggg ccgcctcccc gcctgtccgg atggaagggc taattcactc
    10261 ccaacgaata caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
    10321 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    10381 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    10441 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
    10501 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
    10561 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    10621 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggca
    10681 tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt atgggtatta tgggtctgca
    10741 ttaatgaatc ggccaacgat cccggtgtga aataccgcac agatgcgtaa ggagaaaata
    10801 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
    10861 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
    10921 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
    10981 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    11041 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    11101 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    11161 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    11221 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    11281 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    11341 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    11401 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
    11461 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    11521 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    11581 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    11641 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    11701 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    11761 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    11821 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    11881 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    11941 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    12001 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    12061 tgaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc
    12121 tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag
    12181 agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg
    12241 acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc
    12301 aaagtaaact ggatggcttt ctcgccgcca aggatctgat ggcgcagggg atcaagctct
    12361 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
    12421 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
    12481 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
    12541 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
    12601 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
    12661 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
    12721 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
    12781 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
    12841 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
    12901 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
    12961 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
    13021 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
    13081 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
    13141 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaatttt gttaaaattt
    13201 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaacatcc cttataaatc
    13261 aaaagaatag accgcgatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
    13321 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
    13381 acgtgaacca tcacccaaat caagtttttt gcggtcgagg tgccgtaaag ctctaaatcg
    13441 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
    13501 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
    13561 gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc tacagggcgc gtccattcgc
    13621 cattcaggat cgaattaatt cttaattaac atcatcaata atatacctt
//