pLenti-SV40-T(Neo)

                            LOCUS       dna                     10536 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     Other Gene      715..895
                     /gene="HIV-1_5_LTR other"
     Other Gene      1006..1050
                     /gene="HIV-1_psi_pack other"
     misc_binding    1092..1097
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  1557..1790
                     /gene="RRE reg"
     ORF             1600..2301
                     /sequence="ORF_1 rf(1)"
     Promoter        2885..2966
                     /gene="CMV prom"
     Regulatory_Seq  3022..3061
                     /gene="tetO reg"
     misc_binding    3068..3073
                     /dbxref="REBASE:XmaI"
     misc_binding    3068..3073
                     /dbxref="REBASE:SmaI"
     misc_binding    3074..3079
                     /dbxref="REBASE:ClaI"
     misc_binding    3125..3130
                     /dbxref="REBASE:EcoRI"
     misc_binding    3143..3148
                     /dbxref="REBASE:StuI"
     misc_binding    3155..3160
                     /dbxref="REBASE:EcoRV"
     misc_binding    3161..3166
                     /dbxref="REBASE:ApaI"
     misc_binding    3167..3174
                     /dbxref="REBASE:NotI"
     ORF             3209..5335
                     /sequence="ORF_2 rf(2)"
     Other Gene      3467..5335
                     /gene="SV40_lrgT other"
     misc_binding    5342..5347
                     /dbxref="REBASE:XbaI"
     Promoter        5370..5638
                     /gene="SV40 prom"
     Rep_Origin      5537..5614
                     /gene="SV40 origin"
     misc_binding    5617..5629
                     /dbxref="REBASE:SfiI"
     ORF             5705..6499
                     /sequence="ORF_3 rf(2)"
     Marker          5708..6496
                     /gene="NTP_II marker"
     Other Gene      7109..7161
                     /gene="delta_U3 other"
     Other Gene      7162..7342
                     /gene="HIV-1_5_LTR other"
     Rep_Origin      7848..8467
                     /gene="pBR322 origin"
     Promoter        9120..9169
                     /gene="NEOKAN prom"
     ORF             9258..10052
                     /sequence="ORF_4 rf(3)"
     Marker          9261..10049
                     /gene="NTP_II marker"
     Rep_Origin      10157..10462
                     /gene="f1 origin"
BASE COUNT    2832 a   2337 c   2752 g   2615 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta     
      481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag     
      541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg     
      601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact     
      661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct     
      721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt     
      781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac     
      841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc     
      901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc     
      961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa     
     1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg     
     1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata     
     1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc     
     1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga     
     1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc     
     1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca     
     1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag     
     1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta     
     1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga     
     1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg     
     1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg     
     1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc     
     1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg     
     1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat     
     1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac     
     1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat     
     1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca     
     2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga     
     2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg     
     2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa     
     2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga     
     2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag     
     2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa     
     2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat     
     2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt     
     2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa     
     2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc     
     2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct     
     2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt     
     2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg     
     2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca     
     2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca     
     2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc     
     3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag     
     3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accccagtgt ggtggcctgc     
     3121 aggtgaattc actagtaccg gtaggcctgt cgacgatatc gggcccgcgg ccgctggatc     
     3181 ccgcgaccgg tcgaccagct ttgcaaagat ggataaagtt ttaaacagag aggaatcttt     
     3241 gcagctaatg gaccttctag gtcttgaaag gagtgcctgg gggaatattc ctctgatgag     
     3301 aaaggcatat ttaaaaaaat gcaaggagtt tcatcctgat aaaggaggag atgaagaaaa     
     3361 aatgaagaaa atgaatactc tgtacaagaa aatggaagat ggagtaaaat atgctcatca     
     3421 acctgacttt ggaggcttct gggatgcaac tgagattcca acctatggaa ctgatgaatg     
     3481 ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc     
     3541 tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa     
     3601 ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt     
     3661 tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct     
     3721 atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata acagttataa     
     3781 tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta     
     3841 tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt     
     3901 gatgtatagt gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg     
     3961 gttaaaggag catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa     
     4021 gcttgtaaca gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat     
     4081 gtacttggaa tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc     
     4141 cagccactat aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa     
     4201 aaaccaaaaa accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga     
     4261 tagcctacaa ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag     
     4321 gatggatata atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt     
     4381 tgcttggcta cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg     
     4441 catggtgtac aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg     
     4501 taaaactaca ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa     
     4561 tttgcccttg gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt     
     4621 ttttgaggat gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat     
     4681 taataacctg gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa     
     4741 gaaacaccta aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta     
     4801 cagtgtgcct aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga     
     4861 ttatttaaag cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag     
     4921 tggcattgct ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag     
     4981 tattcagagc agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt     
     5041 gtatcaaaaa atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa     
     5101 cagtgatgat gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga     
     5161 gaagaacatg gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt     
     5221 tcaggcccct cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag     
     5281 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaggatc     
     5341 ctctagaacg cttgagttta aacacgcgtg gtgtggaaag tccccaggct ccccagcagg     
     5401 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg     
     5461 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc     
     5521 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca     
     5581 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt     
     5641 ccagaagtag tgaggaggct tttttggagg ccggtaccgt gacgtggagg agaatcccgg     
     5701 ccctatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct     
     5761 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct     
     5821 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga     
     5881 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc     
     5941 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg     
     6001 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc     
     6061 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca     
     6121 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga     
     6181 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc     
     6241 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga     
     6301 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca     
     6361 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg     
     6421 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct     
     6481 tcttgacgag ttcttctgaa cgcgttccgg aaatcaacct ctggattaca aaatttgtga     
     6541 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt     
     6601 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa     
     6661 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt     
     6721 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct     
     6781 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg     
     6841 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc     
     6901 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg     
     6961 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct     
     7021 gctgccggct ctgcggcctc ttccgcgtct cgccttcgcc ctcagacgag tcggatctcc     
     7081 ctttgggccg cctccccgcc tgtccggatg gaagggctaa ttcactccca acgaatacaa     
     7141 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc     
     7201 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa     
     7261 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag     
     7321 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa     
     7381 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt     
     7441 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct     
     7501 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggcatct atgtcgggtg     
     7561 cggagaaaga ggtaatgaaa tggcattatg ggtattatgg gtctgcatta atgaatcggc     
     7621 caacgatccc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc     
     7681 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta     
     7741 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag     
     7801 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg     
     7861 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg     
     7921 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg     
     7981 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga     
     8041 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc     
     8101 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt     
     8161 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact     
     8221 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg     
     8281 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt     
     8341 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt     
     8401 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct     
     8461 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg     
     8521 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt     
     8581 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt     
     8641 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc     
     8701 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg     
     8761 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc     
     8821 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg     
     8881 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttga aaaaggatct     
     8941 tcacctagat ccttttcacg tagaaagcca gtccgcagaa acggtgctga ccccggatga     
     9001 atgtcagcta ctgggctatc tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag     
     9061 cttgcagtgg gcttacatgg cgatagctag actgggcggt tttatggaca gcaagcgaac     
     9121 cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga     
     9181 tggctttctc gccgccaagg atctgatggc gcaggggatc aagctctgat caagagacag     
     9241 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt     
     9301 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg     
     9361 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg     
     9421 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg     
     9481 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg     
     9541 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca     
     9601 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc     
     9661 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc     
     9721 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca     
     9781 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga     
     9841 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg     
     9901 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg     
     9961 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg     
    10021 ccttctatcg ccttcttgac gagttcttct gaattttgtt aaaatttttg ttaaatcagc     
    10081 tcatttttta accaataggc cgaaatcggc aacatccctt ataaatcaaa agaatagacc     
    10141 gcgatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac     
    10201 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca     
    10261 cccaaatcaa gttttttgcg gtcgaggtgc cgtaaagctc taaatcggaa ccctaaaggg     
    10321 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag     
    10381 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc     
    10441 accacacccg cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggatcga     
    10501 attaattctt aattaacatc atcaataata tacctt                               
//