pLenti-SV40-T-No-Selection

                            LOCUS       dna                      9411 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     Other Gene      715..895
                     /gene="HIV-1_5_LTR other"
     Other Gene      1006..1050
                     /gene="HIV-1_psi_pack other"
     misc_binding    1092..1097
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  1557..1790
                     /gene="RRE reg"
     ORF             1600..2301
                     /sequence="ORF_1 rf(1)"
     Promoter        2885..2966
                     /gene="CMV prom"
     Regulatory_Seq  3022..3061
                     /gene="tetO reg"
     misc_binding    3068..3073
                     /dbxref="REBASE:XmaI"
     misc_binding    3068..3073
                     /dbxref="REBASE:SmaI"
     misc_binding    3074..3079
                     /dbxref="REBASE:ClaI"
     misc_binding    3098..3103
                     /dbxref="REBASE:KpnI"
     misc_binding    3125..3130
                     /dbxref="REBASE:EcoRI"
     misc_binding    3143..3148
                     /dbxref="REBASE:StuI"
     misc_binding    3155..3160
                     /dbxref="REBASE:EcoRV"
     misc_binding    3161..3166
                     /dbxref="REBASE:ApaI"
     misc_binding    3167..3174
                     /dbxref="REBASE:NotI"
     ORF             3209..5335
                     /sequence="ORF_2 rf(2)"
     Other Gene      3467..5335
                     /gene="SV40_lrgT other"
     misc_binding    5369..5374
                     /dbxref="REBASE:XbaI"
     misc_binding    5381..5386
                     /dbxref="REBASE:XhoI"
     Other Gene      5984..6036
                     /gene="delta_U3 other"
     Other Gene      6037..6217
                     /gene="HIV-1_5_LTR other"
     Rep_Origin      6723..7342
                     /gene="pBR322 origin"
     Promoter        7995..8044
                     /gene="NEOKAN prom"
     ORF             8133..8927
                     /sequence="ORF_3 rf(3)"
     Marker          8136..8924
                     /gene="NTP_II marker"
     Rep_Origin      9032..9337
                     /gene="f1 origin"
BASE COUNT    2614 a   2003 c   2422 g   2372 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta     
      481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag     
      541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg     
      601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact     
      661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct     
      721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt     
      781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac     
      841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc     
      901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc     
      961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa     
     1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg     
     1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata     
     1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc     
     1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga     
     1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc     
     1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca     
     1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag     
     1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta     
     1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga     
     1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg     
     1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg     
     1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc     
     1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg     
     1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat     
     1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac     
     1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat     
     1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca     
     2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga     
     2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg     
     2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa     
     2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga     
     2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag     
     2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa     
     2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat     
     2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt     
     2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa     
     2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc     
     2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct     
     2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt     
     2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg     
     2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca     
     2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca     
     2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc     
     3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag     
     3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accccagtgt ggtggcctgc     
     3121 aggtgaattc actagtaccg gtaggcctgt cgacgatatc gggcccgcgg ccgctggatc     
     3181 ccgcgaccgg tcgaccagct ttgcaaagat ggataaagtt ttaaacagag aggaatcttt     
     3241 gcagctaatg gaccttctag gtcttgaaag gagtgcctgg gggaatattc ctctgatgag     
     3301 aaaggcatat ttaaaaaaat gcaaggagtt tcatcctgat aaaggaggag atgaagaaaa     
     3361 aatgaagaaa atgaatactc tgtacaagaa aatggaagat ggagtaaaat atgctcatca     
     3421 acctgacttt ggaggcttct gggatgcaac tgagattcca acctatggaa ctgatgaatg     
     3481 ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc     
     3541 tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa     
     3601 ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt     
     3661 tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct     
     3721 atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata acagttataa     
     3781 tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta     
     3841 tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt     
     3901 gatgtatagt gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg     
     3961 gttaaaggag catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa     
     4021 gcttgtaaca gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat     
     4081 gtacttggaa tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc     
     4141 cagccactat aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa     
     4201 aaaccaaaaa accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga     
     4261 tagcctacaa ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag     
     4321 gatggatata atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt     
     4381 tgcttggcta cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg     
     4441 catggtgtac aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg     
     4501 taaaactaca ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa     
     4561 tttgcccttg gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt     
     4621 ttttgaggat gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat     
     4681 taataacctg gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa     
     4741 gaaacaccta aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta     
     4801 cagtgtgcct aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga     
     4861 ttatttaaag cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag     
     4921 tggcattgct ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag     
     4981 tattcagagc agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt     
     5041 gtatcaaaaa atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa     
     5101 cagtgatgat gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga     
     5161 gaagaacatg gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt     
     5221 tcaggcccct cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag     
     5281 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga     
     5341 atgcaattgt tgttgttaac ggggatcctc tagactgcag ctcgagaatc aacctctgga     
     5401 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg     
     5461 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt     
     5521 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag     
     5581 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc     
     5641 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga     
     5701 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa     
     5761 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac     
     5821 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct     
     5881 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtctcgcct tcgccctcag     
     5941 acgagtcgga tctccctttg ggccgcctcc ccgcctgtcc ggatggaagg gctaattcac     
     6001 tcccaacgaa tacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc     
     6061 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg     
     6121 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc     
     6181 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt     
     6241 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt     
     6301 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt     
     6361 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg     
     6421 catctatgtc gggtgcggag aaagaggtaa tgaaatggca ttatgggtat tatgggtctg     
     6481 cattaatgaa tcggccaacg atcccggtgt gaaataccgc acagatgcgt aaggagaaaa     
     6541 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg     
     6601 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg     
     6661 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag     
     6721 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga     
     6781 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct     
     6841 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc     
     6901 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg     
     6961 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc     
     7021 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca     
     7081 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag     
     7141 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct     
     7201 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc     
     7261 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga     
     7321 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca     
     7381 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat     
     7441 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac     
     7501 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt     
     7561 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt     
     7621 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag     
     7681 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct     
     7741 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt     
     7801 gttgaaaaag gatcttcacc tagatccttt tcacgtagaa agccagtccg cagaaacggt     
     7861 gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa     
     7921 agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat     
     7981 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct     
     8041 gcaaagtaaa ctggatggct ttctcgccgc caaggatctg atggcgcagg ggatcaagct     
     8101 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag     
     8161 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg     
     8221 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca     
     8281 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc     
     8341 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg     
     8401 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg     
     8461 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta     
     8521 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag     
     8581 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac     
     8641 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg     
     8701 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg     
     8761 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg     
     8821 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg     
     8881 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaatt ttgttaaaat     
     8941 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaacat cccttataaa     
     9001 tcaaaagaat agaccgcgat agggttgagt gttgttccag tttggaacaa gagtccacta     
     9061 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca     
     9121 ctacgtgaac catcacccaa atcaagtttt ttgcggtcga ggtgccgtaa agctctaaat     
     9181 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg     
     9241 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc     
     9301 acgctgcgcg taaccaccac acccgcgcgc ttaatgcgcc gctacagggc gcgtccattc     
     9361 gccattcagg atcgaattaa ttcttaatta acatcatcaa taatatacct t              
//