pLenti-SV40-T-tsA58
| Vector Name | pLenti-SV40-T-tsA58 |
| VectorType | Lentivector |
| Antibiotic Information | Mammalian: Puromycin. However, no selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
| Additional Information | No Additional Information |
Double-click on the features for more information
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accccagtgt ggtggcctgc
3121 aggtgaattc agccaccatg gataaagttt taaacagaga ggaatctttg cagctaatgg
3181 accttctagg tcttgaaagg agtgcctggg ggaatattcc tctgatgaga aaggcatatt
3241 taaaaaaatg caaggagttt catcctgata aaggaggaga tgaagaaaaa atgaagaaaa
3301 tgaatactct gtacaagaaa atggaagatg gagtaaaata tgctcatcaa cctgactttg
3361 gaggcttctg ggatgcaact gagattccaa cctatggaac tgatgaatgg gagcagtggt
3421 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
3481 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
3541 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
3601 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
3661 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac
3721 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat
3781 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg
3841 ccttgactag agatccattt tctgttattg aggaaagttt gccaggtggg ttaaaggagc
3901 atgattttaa tccagaagaa gcagaggaaa ctaaacaagt gtcctggaag cttgtaacag
3961 agtatgcaat ggaaacaaaa tgtgatgatg tgttgttatt gcttgggatg tacttggaat
4021 ttcagtacag ttttgaaatg tgtttaaaat gtattaaaaa agaacagccc agccactata
4081 agtaccatga aaagcattat gcaaatgctg ctatatttgc tgacagcaaa aaccaaaaaa
4141 ccatatgcca acaggctgtt gatactgttt tagctaaaaa gcgggttgat agcctacaat
4201 taactagaga acaaatgtta acaaacagat ttaatgatct tttggatagg atggatataa
4261 tgtttggttc tacaggctct gctgacatag aagaatggat ggctggagtt gcttggctac
4321 actgtttgtt gcccaaaatg gattcagtgg tgtatgactt tttaaaatgc atggtgtaca
4381 acattcctaa aaaaagatac tggctgttta aaggaccaat tgatagtggt aaaactacat
4441 tagcagctgt tttgcttgaa ttatgtgggg ggaaagcttt aaatgttaat ttgcccttgg
4501 acaggctgaa ctttgagcta ggagtagcta ttgaccagtt tttagtagtt tttgaggatg
4561 taaagggcac tggaggggag tccagagatt tgccttcagg tcagggaatt aataacctgg
4621 acaatttaag ggattatttg gatggcagtg ttaaggtaaa cttagaaaag aaacacctaa
4681 ataaaagaac tcaaatattt ccccctggaa tagtcaccat gaatgagtac agtgtgccta
4741 aaacactgca ggccagattt gtaaaacaaa tagattttag gcccaaagat tatttaaagc
4801 attgcctgga acgcagtgag tttttgttag aaaagagaat aattcaaagt ggcattgctt
4861 tgcttcttat gttaatttgg tacagacctg tggctgagtt tgctcaaagt attcagagca
4921 gaattgtgga gtggaaagag agattggaca aagagtttag tttgtcagtg tatcaaaaaa
4981 tgaagtttaa tgtggctatg ggaattggag ttttagattg gctaagaaac agtgatgatg
5041 atgatgaaga cagccaggaa aatgctgata aaaatgaaga tggtggggag aagaacatgg
5101 aagactcagg gcatgaaaca ggcattgatt cacagtccca aggctcattt caggcccctc
5161 agtcctcaca gtctgttcat gatcataatc agccatacca catttgtaga ggttttactt
5221 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgctcgagta
5281 cccatacgac gtcccagact acgcttgagt ttaaacacgc gtggtgtgga aagtccccag
5341 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
5401 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
5461 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
5521 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
5581 cctctgagct attccagaag tagtgaggag gcttttttgg aggccatgac cgagtacaag
5641 cccacggtgc gcctcgccac ccgcgacgac gtccctcggg ccgtacgcac cctcgccgcc
5701 gcgttcgccg actaccccgc cacgcgccac accgtggacc cggaccgcca catcgagcgg
5761 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
5821 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
5881 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
5941 cagcaacaga tggaagggct cctggcgccg caccggccca aggagcccgc gtggttcctg
6001 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
6061 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
6121 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
6181 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaacgcg ttccggaaat
6241 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
6301 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
6361 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
6421 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
6481 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
6541 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
6601 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
6661 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
6721 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctcgcc
6781 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgtc cggatggaag
6841 ggctaattca ctcccaacga atacaagatc tgctttttgc ttgtactggg tctctctggt
6901 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
6961 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
7021 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
7081 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
7141 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
7201 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
7261 atcatgtctg gcatctatgt cgggtgcgga gaaagaggta atgaaatggc attatgggta
7321 ttatgggtct gcattaatga atcggccaac gatcccggtg tgaaataccg cacagatgcg
7381 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
7441 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
7501 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
7561 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
7621 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
7681 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
7741 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
7801 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
7861 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
7921 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
7981 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
8041 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
8101 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
8161 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
8221 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
8281 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
8341 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
8401 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
8461 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
8521 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
8581 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
8641 tgcgcaacgt tgttgaaaaa ggatcttcac ctagatcctt ttcacgtaga aagccagtcc
8701 gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa
8761 cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg
8821 ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt
8881 tgggaagccc tgcaaagtaa actggatggc tttctcgccg ccaaggatct gatggcgcag
8941 gggatcaagc tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
9001 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
9061 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
9121 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
9181 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
9241 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
9301 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
9361 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
9421 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
9481 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
9541 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
9601 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
9661 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
9721 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaat
9781 tttgttaaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaca
9841 tcccttataa atcaaaagaa tagaccgcga tagggttgag tgttgttcca gtttggaaca
9901 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
9961 gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttgcggtcg aggtgccgta
10021 aagctctaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
10081 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
10141 gtgtagcggt cacgctgcgc gtaaccacca cacccgcgcg cttaatgcgc cgctacaggg
10201 cgcgtccatt cgccattcag gatcgaatta attcttaatt aacatcatca ataatatacc
10261 tt
LOCUS dna 10262 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
Promoter 2885..2966
/gene="CMV prom"
Regulatory_Seq 3022..3061
/gene="tetO reg"
misc_binding 3068..3073
/dbxref="REBASE:XmaI"
misc_binding 3068..3073
/dbxref="REBASE:SmaI"
misc_binding 3074..3079
/dbxref="REBASE:ClaI"
misc_binding 3098..3103
/dbxref="REBASE:KpnI"
misc_binding 3125..3130
/dbxref="REBASE:EcoRI"
ORF 3138..5264
/sequence="ORF_4 rf(3)"
Other Gene 3396..5264
/gene="SV40_lrgT other"
misc_binding 5273..5278
/dbxref="REBASE:XhoI"
Promoter 5323..5591
/gene="SV40 prom"
Rep_Origin 5490..5567
/gene="SV40 origin"
ORF 5557..6225
/sequence="ORF_2 rf(1)"
misc_binding 5570..5582
/dbxref="REBASE:SfiI"
Marker 5626..6225
/gene="puro marker"
Other Gene 6835..6887
/gene="delta_U3 other"
Other Gene 6888..7068
/gene="HIV-1_5_LTR other"
Rep_Origin 7574..8193
/gene="pBR322 origin"
Promoter 8846..8895
/gene="NEOKAN prom"
ORF 8984..9778
/sequence="ORF_3 rf(2)"
Marker 8987..9775
/gene="NTP_II marker"
Rep_Origin 9883..10188
/gene="f1 origin"