pLenti-SV40-Tt
| Vector Name | pLenti-SV40-Tt |
| VectorType | Lentivector |
| Antibiotic Information | No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
| Additional Information | No Additional Information |
Double-click on the features for more information
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accccagtgt ggtggcctgc
3121 aggtgaattc actagtaccg gtaggcctgt cgactggatc cgcggcctct gagctattcc
3181 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt tgcaaagatg
3241 gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg
3301 agtgcctggg ggaatattcc tctgatgaga aaggcatatt taaaaaaatg caaggagttt
3361 catcctgata aaggaggaga tgaagaaaaa atgaagaaaa tgaatactct gtacaagaaa
3421 atggaagatg gagtaaaata tgctcatcaa cctgactttg gaggcttctg ggatgcaact
3481 gaggtatttg cttcttcctt aaatcctggt gttgatgcaa tgtactgcaa acaatggcct
3541 gagtgtgcaa agaaaatgtc tgctaactgc atatgcttgc tgtgcttact gaggatgaag
3601 catgaaaata gaaaattata caggaaagat ccacttgtgt gggttgattg ctactgcttc
3661 gattgcttta gaatgtggtt tggacttgat ctttgtgaag gaaccttact tctgtggtgt
3721 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt
3781 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta
3841 tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc
3901 agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc
3961 aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt
4021 gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga
4081 aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag
4141 gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc
4201 tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt
4261 taataaggaa tatttgatgt atagtgcctt gactagagat ccattttctg ttattgagga
4321 aagtttgcca ggtgggttaa aggagcatga ttttaatcca gaagaagcag aggaaactaa
4381 acaagtgtcc tggaagcttg taacagagta tgcaatggaa acaaaatgtg atgatgtgtt
4441 gttattgctt gggatgtact tggaatttca gtacagtttt gaaatgtgtt taaaatgtat
4501 taaaaaagaa cagcccagcc actataagta ccatgaaaag cattatgcaa atgctgctat
4561 atttgctgac agcaaaaacc aaaaaaccat atgccaacag gctgttgata ctgttttagc
4621 taaaaagcgg gttgatagcc tacaattaac tagagaacaa atgttaacaa acagatttaa
4681 tgatcttttg gataggatgg atataatgtt tggttctaca ggctctgctg acatagaaga
4741 atggatggct ggagttgctt ggctacactg tttgttgccc aaaatggatt cagtggtgta
4801 tgacttttta aaatgcatgg tgtacaacat tcctaaaaaa agatactggc tgtttaaagg
4861 accaattgat agtggtaaaa ctacattagc agctgctttg cttgaattat gtggggggaa
4921 agctttaaat gttaatttgc ccttggacag gctgaacttt gagctaggag tagctattga
4981 ccagttttta gtagtttttg aggatgtaaa gggcactgga ggggagtcca gagatttgcc
5041 ttcaggtcag ggaattaata acctggacaa tttaagggat tatttggatg gcagtgttaa
5101 ggtaaactta gaaaagaaac acctaaataa aagaactcaa atatttcccc ctggaatagt
5161 caccatgaat gagtacagtg tgcctaaaac actgcaggcc agatttgtaa aacaaataga
5221 ttttaggccc aaagattatt taaagcattg cctggaacgc agtgagtttt tgttagaaaa
5281 gagaataatt caaagtggca ttgctttgct tcttatgtta atttggtaca gacctgtggc
5341 tgagtttgct caaagtattc agagcagaat tgtggagtgg aaagagagat tggacaaaga
5401 gtttagtttg tcagtgtatc aaaaaatgaa gtttaatgtg gctatgggaa ttggagtttt
5461 agattggcta agaaacagtg atgatgatga tgaagacagc caggaaaatg ctgataaaaa
5521 tgaagatggt ggggagaaga acatggaaga ctcagggcat gaaacaggca ttgattcaca
5581 gtcccaaggc tcatttcagg cccctcagtc ctcacagtct gttcatgatc ataatcagcc
5641 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
5701 tgaaacataa aatgaatgca attgttgttg ttggatccgg taccgaattc gcggccgcac
5761 tcgagatatc tagactgcag ctcgagtacc catacgacgt cccagactac gcttgagttt
5821 aaacacgcgt ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
5881 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
5941 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
6001 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
6061 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
6121 ttttttggag gccatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
6181 ccctcgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
6241 cgtggacccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
6301 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
6361 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
6421 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaagggctcc tggcgccgca
6481 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
6541 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
6601 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
6661 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
6721 gcccggtgcc tgaacgcgtt ccggaaatca acctctggat tacaaaattt gtgaaagatt
6781 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
6841 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
6901 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
6961 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
7021 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
7081 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
7141 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
7201 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
7261 ggctctgcgg cctcttccgc gtctcgcctt cgccctcaga cgagtcggat ctccctttgg
7321 gccgcctccc cgcctgtccg gatggaaggg ctaattcact cccaacgaat acaagatctg
7381 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
7441 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
7501 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
7561 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
7621 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
7681 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
7741 ggtttgtcca aactcatcaa tgtatcttat catgtctggc atctatgtcg ggtgcggaga
7801 aagaggtaat gaaatggcat tatgggtatt atgggtctgc attaatgaat cggccaacga
7861 tcccggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
7921 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
7981 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
8041 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
8101 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
8161 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
8221 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
8281 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
8341 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
8401 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
8461 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
8521 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
8581 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
8641 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
8701 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
8761 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
8821 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
8881 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
8941 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
9001 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
9061 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
9121 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgaaaaagg atcttcacct
9181 agatcctttt cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca
9241 gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca
9301 gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat
9361 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
9421 tctcgccgcc aaggatctga tggcgcaggg gatcaagctc tgatcaagag acaggatgag
9481 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
9541 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
9601 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
9661 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
9721 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
9781 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
9841 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
9901 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
9961 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
10021 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
10081 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
10141 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
10201 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
10261 atcgccttct tgacgagttc ttctgaattt tgttaaaatt tttgttaaat cagctcattt
10321 tttaaccaat aggccgaaat cggcaacatc ccttataaat caaaagaata gaccgcgata
10381 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
10441 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcacccaaa
10501 tcaagttttt tgcggtcgag gtgccgtaaa gctctaaatc ggaaccctaa agggagcccc
10561 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
10621 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
10681 cccgcgcgct taatgcgccg ctacagggcg cgtccattcg ccattcagga tcgaattaat
10741 tcttaattaa catcatcaat aatatacctt
LOCUS dna 10770 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
Promoter 2885..2966
/gene="CMV prom"
Regulatory_Seq 3022..3061
/gene="tetO reg"
misc_binding 3068..3073
/dbxref="REBASE:XmaI"
misc_binding 3068..3073
/dbxref="REBASE:SmaI"
misc_binding 3074..3079
/dbxref="REBASE:ClaI"
Other Gene 3763..3778
/gene="SV40_int other"
Other Gene 3842..5710
/gene="SV40_lrgT other"
ORF 3908..5710
/sequence="ORF_2 rf(2)"
misc_binding 5751..5758
/dbxref="REBASE:NotI"
misc_binding 5765..5770
/dbxref="REBASE:EcoRV"
misc_binding 5769..5774
/dbxref="REBASE:XbaI"
Promoter 5831..6099
/gene="SV40 prom"
Rep_Origin 5998..6075
/gene="SV40 origin"
ORF 6065..6733
/sequence="ORF_3 rf(2)"
misc_binding 6078..6090
/dbxref="REBASE:SfiI"
Marker 6134..6733
/gene="puro marker"
Other Gene 7343..7395
/gene="delta_U3 other"
Other Gene 7396..7576
/gene="HIV-1_5_LTR other"
Rep_Origin 8082..8701
/gene="pBR322 origin"
Promoter 9354..9403
/gene="NEOKAN prom"
ORF 9492..10286
/sequence="ORF_4 rf(3)"
Marker 9495..10283
/gene="NTP_II marker"
Rep_Origin 10391..10696
/gene="f1 origin"