pLenti-U6-sgRNA-EF1a-FnCas12a-2A-GFP

                            LOCUS       dna                     12850 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    457..462
                     /dbxref="REBASE:HpaI"
     Other Gene      715..895
                     /gene="HIV-1_5_LTR other"
     Other Gene      1006..1050
                     /gene="HIV-1_psi_pack other"
     misc_binding    1092..1097
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  1557..1790
                     /gene="RRE reg"
     ORF             1600..2301
                     /sequence="ORF_1 rf(1)"
     ORF             2749..3687
                     /sequence="ORF_4 rf(5)"
     misc_binding    2753..2758
                     /dbxref="REBASE:XbaI"
     Promoter        2770..3947
                     /gene="EF1a prom"
     misc_binding    3382..3387
                     /dbxref="REBASE:ApaI"
     ORF             3966..8807
                     /sequence="ORF_3 rf(3)"
     misc_binding    4347..4352
                     /dbxref="REBASE:ClaI"
     misc_binding    7914..7919
                     /dbxref="REBASE:BamHI"
     Tag             7920..7946
                     /gene="HA tag"
     misc_binding    8082..8087
                     /dbxref="REBASE:EcoRI"
     Reporter        8088..8804
                     /gene="EGFP reporter"
     Other Gene      9423..9475
                     /gene="delta_U3 other"
     Other Gene      9476..9656
                     /gene="HIV-1_5_LTR other"
     Rep_Origin      10162..10781
                     /gene="pBR322 origin"
     Promoter        11434..11483
                     /gene="NEOKAN prom"
     ORF             11572..12366
                     /sequence="ORF_2 rf(1)"
     Marker          11575..12363
                     /gene="NTP_II marker"
     Rep_Origin      12471..12776
                     /gene="f1 origin"
BASE COUNT    3526 a   2989 c   3386 g   2949 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta     
      481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag     
      541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg     
      601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact     
      661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct     
      721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt     
      781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac     
      841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc     
      901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc     
      961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa     
     1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg     
     1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata     
     1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc     
     1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga     
     1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc     
     1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca     
     1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag     
     1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta     
     1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga     
     1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg     
     1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg     
     1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc     
     1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg     
     1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat     
     1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac     
     1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat     
     1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca     
     2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga     
     2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg     
     2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa     
     2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga     
     2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag     
     2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa     
     2401 aattcaaaat tttcgggttt ttcgaaccta gggagggcct atttcccatg attccttcat     
     2461 atttgcatat acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca     
     2521 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag     
     2581 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg     
     2641 atttcttggc tttatatatc ttgtggaaag gacgaaacac cgtctaagaa ctttaaataa     
     2701 tttctactgt tgtagatttg tcttcctcga ggaagacaat ttttttagtc agtctagaga     
     2761 gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag     
     2821 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac     
     2881 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat     
     2941 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag     
     3001 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg     
     3061 ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa     
     3121 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg     
     3181 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc     
     3241 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt     
     3301 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt     
     3361 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg     
     3421 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc     
     3481 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt     
     3541 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa     
     3601 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg     
     3661 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc     
     3721 acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt     
     3781 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact     
     3841 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc     
     3901 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga atttcgactg     
     3961 ctagcatgag catctaccag gagttcgtca acaagtattc actgagtaag acactgcggt     
     4021 tcgagctgat cccacagggc aagacactgg agaacatcaa ggcccgaggc ctgattctgg     
     4081 acgatgagaa gcgggcaaaa gactataaga aagccaagca gatcattgat aaataccacc     
     4141 agttctttat cgaggaaatt ctgagctccg tgtgcatcag tgaggatctg ctgcagaatt     
     4201 actcagacgt gtacttcaag ctgaagaaga gcgacgatga caacctgcag aaggacttca     
     4261 agtccgccaa ggacaccatc aagaaacaga ttagcgagta catcaaggac tccgaaaagt     
     4321 ttaaaaatct gttcaaccag aatctgatcg atgctaagaa aggccaggag tccgacctga     
     4381 tcctgtggct gaaacagtct aaggacaatg ggattgaact gttcaaggct aactccgata     
     4441 tcactgatat tgacgaggca ctggaaatca tcaagagctt caagggatgg accacatact     
     4501 ttaaaggctt ccacgagaac cgcaagaacg tgtactccag caacgacatt cctacctcca     
     4561 tcatctaccg aatcgtcgat gacaatctgc caaagttcct ggagaacaag gccaaatatg     
     4621 aatctctgaa ggacaaagct cccgaggcaa ttaattacga acagatcaag aaagatctgg     
     4681 ctgaggaact gacattcgat atcgactata agactagcga ggtgaaccag agggtctttt     
     4741 ccctggacga ggtgtttgaa atcgccaatt tcaacaatta cctgaaccag tccggcatta     
     4801 ctaaattcaa taccatcatt ggcgggaagt ttgtgaacgg ggagaatacc aagcgcaagg     
     4861 gaattaacga atacatcaat ctgtatagcc agcagatcaa cgacaaaact ctgaagaaat     
     4921 acaagatgtc tgtgctgttc aaacagatcc tgagtgatac cgagtccaag tcttttgtca     
     4981 ttgataaact ggaagatgac tcagacgtgg tcactaccat gcagagcttt tatgagcaga     
     5041 tcgccgcttt caagacagtg gaggaaaaat ctattaagga aactctgagt ctgctgttcg     
     5101 atgacctgaa agcccagaag ctggacctga gtaagatcta cttcaaaaac gataagagtc     
     5161 tgacagacct gtcacagcag gtgtttgatg actattccgt gattgggacc gccgtcctgg     
     5221 agtacattac acagcagatc gctccaaaga acctggataa tccctctaag aaagagcagg     
     5281 aactgatcgc taagaaaacc gagaaggcaa aatatctgag tctggaaaca attaagctgg     
     5341 cactggagga gttcaacaag cacagggata ttgacaaaca gtgccgcttt gaggaaatcc     
     5401 tggccaactt cgcagccatc cccatgattt ttgatgagat cgcccagaac aaagacaatc     
     5461 tggctcagat cagtattaag taccagaacc agggcaagaa agacctgctg caggcttcag     
     5521 cagaagatga cgtgaaagcc atcaaggatc tgctggacca gaccaacaat ctgctgcaca     
     5581 agctgaaaat cttccatatt agtcagtcag aggataaggc taatatcctg gataaagacg     
     5641 aacacttcta cctggtgttc gaggaatgtt acttcgagct ggcaaacatt gtccccctgt     
     5701 ataacaagat taggaactac atcacacaga agccttactc tgacgagaag tttaaactga     
     5761 acttcgaaaa tagtaccctg gccaacgggt gggataagaa caaggagcct gacaacacag     
     5821 ctatcctgtt catcaaggat gacaagtact atctgggagt gatgaataag aaaaacaata     
     5881 agatcttcga tgacaaagcc attaaggaga acaaagggga aggatacaag aaaatcgtgt     
     5941 ataagctgct gcccggcgca aataagatgc tgcctaaggt gttcttcagc gccaagagta     
     6001 tcaaattcta caacccatcc gaggacatcc tgcggattag aaatcactca acacatacta     
     6061 agaacgggag cccccagaag ggatatgaga aatttgagtt caacatcgag gattgcagga     
     6121 agtttattga cttctacaag cagagcatct ccaaacaccc tgaatggaag gattttggct     
     6181 tccggttttc cgacacacag agatataact ctatcgacga gttctaccgc gaggtggaaa     
     6241 atcaggggta taagctgact tttgagaaca tttctgaaag ttacatcgac agcgtggtca     
     6301 atcagggaaa gctgtacctg ttccagatct ataacaaaga tttttcagca tacagcaagg     
     6361 gcagaccaaa cctgcataca ctgtactgga aggccctgtt cgatgagagg aatctgcagg     
     6421 acgtggtcta taaactgaac ggagaggccg aactgtttta ccggaagcag tctattccta     
     6481 agaaaatcac tcacccagct aaggaggcca tcgctaacaa gaacaaggac aatcctaaga     
     6541 aagagagcgt gttcgaatac gatctgatta aggacaagcg gttcaccgaa gataagttct     
     6601 ttttccattg tccaatcacc attaacttca agtcaagcgg cgctaacaag ttcaacgacg     
     6661 agatcaatct gctgctgaag gaaaaagcaa acgatgtgca catcctgagc attgaccgag     
     6721 gagagcggca tctggcctac tataccctgg tggatggcaa agggaatatc attaagcagg     
     6781 atacattcaa catcattggc aatgaccgga tgaaaaccaa ctaccacgat aaactggctg     
     6841 caatcgagaa ggatagagac tcagctagga aggactggaa gaaaatcaac aacattaagg     
     6901 agatgaagga aggctatctg agccaggtgg tccatgagat tgcaaagctg gtcatcgaat     
     6961 acaatgccat tgtggtgttc gaggatctga acttcggctt taagaggggg cgctttaagg     
     7021 tggaaaaaca ggtctatcag aagctggaga aaatgctgat cgaaaagctg aattacctgg     
     7081 tgtttaaaga taacgagttc gacaagaccg gaggcgtcct gagagcctac cagctgacag     
     7141 ctccctttga aactttcaag aaaatgggaa aacagacagg catcatctac tatgtgccag     
     7201 ccggattcac ttccaagatc tgccccgtga ccggctttgt caaccagctg taccctaaat     
     7261 atgagtcagt gagcaagtcc caggaatttt tcagcaagtt cgataagatc tgttataatc     
     7321 tggacaaggg gtacttcgag ttttccttcg attacaagaa cttcggcgac aaggccgcta     
     7381 aggggaaatg gaccattgcc tccttcggat ctcgcctgat caactttcga aattccgata     
     7441 aaaaccacaa ttgggacact agggaggtgt acccaaccaa ggagctggaa aagctgctga     
     7501 aagactactc tatcgagtat ggacatggcg aatgcatcaa ggcagccatc tgtggcgaga     
     7561 gtgataagaa atttttcgcc aagctgacct cagtgctgaa tacaatcctg cagatgcgga     
     7621 actcaaagac cgggacagaa ctggactatc tgattagccc cgtggctgat gtcaacggaa     
     7681 acttcttcga cagcagacag gcacccaaaa atatgcctca ggatgcagac gccaacgggg     
     7741 cctaccacat cgggctgaag ggactgatgc tgctgggccg gatcaagaac aatcaggagg     
     7801 ggaagaagct gaacctggtc attaagaacg aggaatactt cgagtttgtc cagaatagaa     
     7861 ataacaaaag gccggcggcc acgaaaaagg ccggccaggc aaaaaagaaa aagggatcct     
     7921 acccatacga tgttccagat tacgcttatc cctacgacgt gcctgattat gcatacccat     
     7981 atgatgtccc cgactatgcc ccaaagaaga agcggaaggt ccagtgtact aattatgctc     
     8041 tcttgaaatt ggctggagat gttgagagca acccaggtcc cgaattcatg gtgagcaagg     
     8101 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg     
     8161 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc     
     8221 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc     
     8281 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct     
     8341 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg     
     8401 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg     
     8461 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca     
     8521 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga     
     8581 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc     
     8641 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc     
     8701 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg     
     8761 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaagtc gacacgcgtt     
     8821 ccggaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg     
     8881 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt     
     8941 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg     
     9001 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc     
     9061 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc     
     9121 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc     
     9181 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc     
     9241 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg     
     9301 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc     
     9361 gtctcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgtccg     
     9421 gatggaaggg ctaattcact cccaacgaat acaagatctg ctttttgctt gtactgggtc     
     9481 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct     
     9541 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga     
     9601 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag     
     9661 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag     
     9721 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa     
     9781 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa     
     9841 tgtatcttat catgtctggc atctatgtcg ggtgcggaga aagaggtaat gaaatggcat     
     9901 tatgggtatt atgggtctgc attaatgaat cggccaacga tcccggtgtg aaataccgca     
     9961 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc     
    10021 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg     
    10081 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa     
    10141 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga     
    10201 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag     
    10261 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct     
    10321 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg     
    10381 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc     
    10441 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt     
    10501 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta     
    10561 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac     
    10621 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc     
    10681 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat     
    10741 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc     
    10801 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt     
    10861 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta     
    10921 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct     
    10981 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg     
    11041 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga     
    11101 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt     
    11161 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt     
    11221 taatagtttg cgcaacgttg ttgaaaaagg atcttcacct agatcctttt cacgtagaaa     
    11281 gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc tatctggaca     
    11341 agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac atggcgatag     
    11401 ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg ggcgccctct     
    11461 ggtaaggttg ggaagccctg caaagtaaac tggatggctt tctcgccgcc aaggatctga     
    11521 tggcgcaggg gatcaagctc tgatcaagag acaggatgag gatcgtttcg catgattgaa     
    11581 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac     
    11641 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg     
    11701 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag     
    11761 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt     
    11821 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg     
    11881 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg     
    11941 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga     
    12001 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag     
    12061 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat     
    12121 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt     
    12181 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg     
    12241 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt     
    12301 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc     
    12361 ttctgaattt tgttaaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat     
    12421 cggcaacatc ccttataaat caaaagaata gaccgcgata gggttgagtg ttgttccagt     
    12481 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt     
    12541 ctatcagggc gatggcccac tacgtgaacc atcacccaaa tcaagttttt tgcggtcgag     
    12601 gtgccgtaaa gctctaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg     
    12661 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc     
    12721 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgcgcgct taatgcgccg     
    12781 ctacagggcg cgtccattcg ccattcagga tcgaattaat tcttaattaa catcatcaat     
    12841 aatatacctt