pLenti-U6-sgRNA-PGK-Neo
| Vector Name | pLenti-U6-sgRNA-PGK-Neo |
| VectorType | Lentiviral Vector |
| Antibiotic Information | Bacterial: Ampicillin Mammalian: Neomycin/G418 |
| Sequencing Primers | U6 Forward Primer: 5'--GAGGGCCTATTTCCCATGATTCC--3' |
| Additional Information | No Additional Information |
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***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt
4021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
4081 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
4141 tcacgagact agcctcgagc cccagtggaa agacgcgcag gcaaaacgca ccacgtgacg
4201 gagcgtgacc gcgcgccgag cgcgcgccaa ggtcgggcag gaagagggcc tatttcccat
4261 gattccttca tatttgcata tacgatacaa ggctgttaga gagataatta gaattaattt
4321 gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg
4381 gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg
4441 aaagtatttc gatttcttgg gtttatatat cttgtggaaa ggacgaaaca ccggttttag
4501 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
4561 agtcggtgct ttttttctcg agagctagcg aattcccacg gggttggggt tgcgcctttt
4621 ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg
4681 cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat
4741 cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga
4801 aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag
4861 taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt
4921 ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg
4981 ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt
5041 ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct
5101 ctctccccag gggaggaccg gtcgcgatcg ccatggctga acaagatgga ttgcacgcag
5161 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
5221 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
5281 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
5341 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
5401 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
5461 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
5521 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
5581 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
5641 tgttcgccag gctcaaggcg cgcatgcccg acggcgatga tctcgtcgtg acccatggcg
5701 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
5761 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
5821 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
5881 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagtt taaacgtcga
5941 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
6001 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
6061 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
6121 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
6181 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
6241 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
6301 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
6361 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
6421 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
6481 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga
6541 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
6601 aagaaaaggg gggactggaa gggctaattc actcccaacg agacaagatc tgctttttgc
6661 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
6721 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
6781 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
6841 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
6901 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
6961 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
7021 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
7081 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
7141 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
7201 ttttgcgtcg agacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
7261 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
7321 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
7381 cccaacagtt gcgcagcctg aatggcgaat ggcgcgacgc gccctgtagc ggcgcattaa
7441 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
7501 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
7561 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
7621 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
7681 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
7741 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
7801 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
7861 cgtttacaat ttcc
LOCUS dna 7874 bp
FEATURES Location/Qualifiers
Promoter 62..90
/gene="amp prom"
Marker 132..992
/gene="amp marker"
ORF 132..992
/sequence="ORF_1 rf(3)"
Rep_Origin 1147..1766
/gene="pBR322 origin"
Promoter 2075..2104
/gene="lac prom"
Promoter 2174..2193
/gene="T3 prom"
Other Gene 2448..2628
/gene="HIV-1_5_LTR other"
Other Gene 2736..2780
/gene="HIV-1_psi_pack other"
misc_binding 3134..3141
/dbxref="REBASE:NotI"
Regulatory_Seq 3290..3523
/gene="RRE reg"
misc_binding 4012..4017
/dbxref="REBASE:HpaI"
misc_binding 4136..4141
/dbxref="REBASE:ClaI"
Promoter 4229..4485
/gene="hU6 prom"
misc_binding 4421..4426
/dbxref="REBASE:NdeI"
ORF 5133..5927
/sequence="ORF_2 rf(3)"
Marker 5138..5924
/gene="NTP_II marker"
misc_binding 5310..5315
/dbxref="REBASE:PstI"
misc_binding 6545..6550
/dbxref="REBASE:KpnI"
Other Gene 6616..6667
/gene="delta_U3 other"
Other Gene 6668..6848
/gene="HIV-1_5_LTR other"
Rep_Origin 7060..7137
/gene="SV40 origin"
misc_binding 7140..7152
/dbxref="REBASE:SfiI"
misc_binding 7191..7196
/dbxref="REBASE:StuI"
Promoter 7228..7246
/gene="T7 prom"
Reporter 7254..7412
/gene="lacZ_a reporter"
Rep_Origin 7434..7740
/gene="f1 origin"
BASE COUNT 1955 a 1912 c 2074 g 1933 t 0 others