pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
| Vector Name | pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro |
| VectorType | Lentiviral Vector |
| Antibiotic Information | Bacterial: Ampicillin Mammalian: Puromycin |
| Sequencing Primers | U6 Forward Primer: 5'--GAGGGCCTATTTCCCATGATTCC--3' |
| Additional Information | No Additional Information |
Double-click on the features for more information
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt
4021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
4081 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
4141 tcacgagact agcctcgagc cccagtggaa agacgcgcag gcaaaacgca ccacgtgacg
4201 gagcgtgacc gcgcgccgag cgcgcgccaa ggtcgggcag gaagagggcc tatttcccat
4261 gattccttca tatttgcata tacgatacaa ggctgttaga gagataatta gaattaattt
4321 gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg
4381 gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg
4441 aaagtatttc gatttcttgg gtttatatat cttgtggaaa ggacgaaaca ccggcacggg
4501 cagcttgccg ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
4561 cttgaaaaag tggcaccgag tcggtgcttt ttttctcgag agctagctgc agtaacgcca
4621 ttttgcaagg catggaaaaa taccaaacca agaatagaga agttcagatc aagggcgggt
4681 acatgaaaat agctaacgtt gggccaaaca ggatatctgc ggtgagcagt ttcggccccg
4741 gcccggggcc aagaacagat ggtcaccgca gtttcggccc cggcccgagg ccaagaacag
4801 atggtcccca gatatggccc aaccctcagc agtttcttaa gacccatcag atgtttccag
4861 gctcccccaa ggacctgaaa tgaccctgcg ccttatttga attaaccaat cagcctgctt
4921 ctcgcttctg ttcgcgcgct tctgcttccc gagctctata aaagagctca caacccctca
4981 ctcggcgcgc cagtcctccg acagactgag tcgcccgggt accgcgatcg ccatggcccc
5041 aaagaagaag cggaaggtcg gtatccacgg agtcccagca gccgacaaga agtacagcat
5101 cggcctggac atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt
5161 gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct
5221 gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac
5281 cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag
5341 caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt
5401 ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc
5461 ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga
5521 caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca
5581 cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca
5641 gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga
5701 cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc
5761 ccagctgccc ggcgagaaga agaatggcct gttcggcaac ctgattgccc tgagcctggg
5821 cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag
5881 caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc
5941 cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag
6001 agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga
6061 gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta
6121 caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc
6181 cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga
6241 ggaactgctc gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa
6301 cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga
6361 agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg
6421 catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag
6481 aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc
6541 cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt
6601 gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt
6661 gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc
6721 catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga
6781 ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt
6841 caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct
6901 ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga
6961 ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt
7021 gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat
7081 caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg
7141 cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga
7201 catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct
7261 ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct
7321 cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa
7381 ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg
7441 catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca
7501 gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga
7561 actggacatc aaccggctgt ccgactacga tgtggaccat atcgtgcctc agagctttct
7621 gaaggacgac tccatcgaca acaaggtgct gaccagaagc gacaagaacc ggggcaagag
7681 cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct
7741 gaacgccaag ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg
7801 cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat
7861 cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga
7921 caagctgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg
7981 gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc
8041 ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga
8101 gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca
8161 ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa
8221 gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg
8281 cgaaaccggg gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct
8341 gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa
8401 agagtctatc ctgcccaaga ggaacagcga taagctgatc gccagaaaga aggactggga
8461 ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc
8521 caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac
8581 catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta
8641 caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga
8701 aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc
8761 cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg
8821 ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga
8881 cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct
8941 ggacaaagtg ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga
9001 gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt
9061 tgacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct
9121 gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg
9181 cgacaaaagg ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa agacgcgtca
9241 gtgtactaat tatgctctct tgaaattggc tggagatgtt gagagcaacc caggtcccat
9301 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
9361 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
9421 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
9481 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
9541 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
9601 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
9661 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
9721 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
9781 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
9841 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagt
9901 ttaaacgtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
9961 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
10021 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
10081 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
10141 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
10201 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
10261 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
10321 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
10381 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
10441 cttccgcgtc tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
10501 ctggaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
10561 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gagacaagat
10621 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
10681 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
10741 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
10801 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
10861 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
10921 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
10981 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
11041 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
11101 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
11161 gaggcctagg cttttgcgtc gagacgtacc caattcgccc tatagtgagt cgtattacgc
11221 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
11281 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
11341 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag
11401 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
11461 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
11521 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
11581 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
11641 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
11701 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
11761 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
11821 caaaatatta acgtttacaa tttcc
LOCUS dna 11845 bp
FEATURES Location/Qualifiers
Promoter 62..90
/gene="amp prom"
Marker 132..992
/gene="amp marker"
ORF 132..992
/sequence="ORF_2 rf(3)"
Rep_Origin 1147..1766
/gene="pBR322 origin"
Promoter 2075..2104
/gene="lac prom"
Promoter 2174..2193
/gene="T3 prom"
Other Gene 2448..2628
/gene="HIV-1_5_LTR other"
Other Gene 2736..2780
/gene="HIV-1_psi_pack other"
misc_binding 2822..2827
/dbxref="REBASE:NruI"
misc_binding 3134..3141
/dbxref="REBASE:NotI"
Regulatory_Seq 3290..3523
/gene="RRE reg"
misc_binding 4012..4017
/dbxref="REBASE:HpaI"
misc_binding 4136..4141
/dbxref="REBASE:ClaI"
Promoter 4229..4485
/gene="hU6 prom"
misc_binding 4421..4426
/dbxref="REBASE:NdeI"
ORF 4910..7600
/sequence="ORF_3 rf(4)"
ORF 5033..9898
/sequence="ORF_1 rf(2)"
misc_binding 6436..6441
/dbxref="REBASE:ApaI"
Marker 9299..9898
/gene="puro marker"
misc_binding 10504..10509
/dbxref="REBASE:EcoRI"
Other Gene 10587..10638
/gene="delta_U3 other"
Other Gene 10639..10819
/gene="HIV-1_5_LTR other"
Rep_Origin 11031..11108
/gene="SV40 origin"
misc_binding 11111..11123
/dbxref="REBASE:SfiI"
Promoter 11199..11217
/gene="T7 prom"
Reporter 11225..11383
/gene="lacZ_a reporter"
Rep_Origin 11405..11711
/gene="f1 origin"