pLenti-hTERT

Vector Name pLenti-hTERT
VectorType Lentiviral Vector
Antibiotic Information No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds.
Sequencing Primers CMV sequencing primer
5`---CGC AAA TGG GCG GTA GGC GTG---3`
SV40 reverse sequencing primer
5`---TAG TCA GCC ATG GGG CGG AGA ---3`
Additional Information No Additional Information
Double-click on the features for more information
***Note: The vector sequence shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page. The restriction sites within the cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc
541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt aatgtagtct
661 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
721 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
781 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
841 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
901 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
961 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
1021 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
1081 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
1141 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
1201 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
1261 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
1321 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
1381 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
1441 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
1501 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
1561 cacagcaagc gccgctgatc ttcagacctg gaggaggaga tatgagggac aattgagaag
1621 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1681 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1741 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1801 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1861 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1921 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1981 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
2041 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
2101 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
2161 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
2221 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
2281 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
2341 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
2401 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgataag cttgggagtt
2461 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
2521 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
2581 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
2641 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
2701 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
2761 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
2821 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
2881 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
2941 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
3001 acgccatcca cgctgttttg acctccatag aagaaccgag tttaaactcc ctatcagtga
3061 tagagatctc cctatcagtg atagagagct agccccggga tcgatcaatt gagtacttac
3121 gtaggtaccc cagtgtggtg gcctgcaggt gaattccacc atgccgcgcg ctccccgctg
3181 ccgagccgtg cgctccctgc tgcgcagcca ctaccgcgag gtgctgccgc tggccacgtt
3241 cgtgcggcgc ctggggcccc agggctggcg gctggtgcag cgcggggacc cggcggcttt
3301 ccgcgcgctg gtggcccagt gcctggtgtg cgtgccctgg gacgcacggc cgccccccgc
3361 cgccccctcc ttccgccagg tgtcctgcct gaaggagctg gtggcccgag tgctgcagag
3421 gctgtgcgag cgcggcgcga agaacgtgct ggccttcggc ttcgcgctgc tggacggggc
3481 ccgcgggggc ccccccgagg ccttcaccac cagcgtgcgc agctacctgc ccaacacggt
3541 gaccgacgca ctgcggggga gcggggcgtg ggggctgctg ctgcgccgcg tgggcgacga
3601 cgtgctggtt cacctgctgg cacgctgcgc gctctttgtg ctggtggctc ccagctgcgc
3661 ctaccaggtg tgcgggccgc cgctgtacca gctcggcgct gccactcagg cccggccccc
3721 gccacacgct agtggacccc gaaggcgtct gggatgcgaa cgggcctgga accatagcgt
3781 cagggaggcc ggggtccccc tgggcctgcc agccccgggt gcgaggaggc gcgggggcag
3841 tgccagccga agtctgccgt tgcccaagag gcccaggcgt ggcgctgccc ctgagccgga
3901 gcggacgccc gttgggcagg ggtcctgggc ccacccgggc aggacgcgtg gaccgagtga
3961 ccgtggtttc tgtgtggtgt cacctgccag acccgccgaa gaagccacct ctttggaggg
4021 tgcgctctct ggcacgcgcc actcccaccc atccgtgggc cgccagcacc acgcgggccc
4081 cccatccaca tcgcggccac cacgtccctg ggacacgcct tgtcccccgg tgtacgccga
4141 gaccaagcac ttcctctact cctcaggcga caaggagcag ctgcggccct ccttcctact
4201 cagctctctg aggcccagcc tgactggcgc tcggaggctc gtggagacca tctttctggg
4261 ttccaggccc tggatgccag ggactccccg caggttgccc cgcctgcccc agcgctactg
4321 gcaaatgcgg cccctgtttc tggagctgct tgggaaccac gcgcagtgcc cctacggggt
4381 gctcctcaag acgcactgcc cgctgcgagc tgcggtcacc ccagcagccg gtgtctgtgc
4441 ccgggagaag ccccagggct ctgtggcggc ccccgaggag gaggacacag acccccgtcg
4501 cctggtgcag ctgctccgcc agcacagcag cccctggcag gtgtacggct tcgtgcgggc
4561 ctgcctgcgc cggctggtgc ccccaggcct ctggggctcc aggcacaacg aacgccgctt
4621 cctcaggaac accaagaagt tcatctccct ggggaagcat gccaagctct cgctgcagga
4681 gctgacgtgg aagatgagcg tgcgggactg cgcttggctg cgcaggagcc caggggttgg
4741 ctgtgttccg gccgcagagc accgtctgcg tgaggagatc ctggccaagt tcctgcactg
4801 gctgatgagt gtgtacgtcg tcgagctgct caggtctttc ttttatgtca cggagaccac
4861 gtttcaaaag aacaggctct ttttctaccg gaagagtgtc tggagcaagt tgcaaagcat
4921 tggaatcaga cagcacttga agagggtgca gctgcgggag ctgtcggaag cagaggtcag
4981 gcagcatcgg gaagccaggc ccgccctgct gacgtccaga ctccgcttca tccccaagcc
5041 tgacgggctg cggccgattg tgaacatgga ctacgtcgtg ggagccagaa cgttccgcag
5101 agaaaagagg gccgagcgtc tcacctcgag ggtgaaggca ctgttcagcg tgctcaacta
5161 cgagcgggcg cggcgccccg gcctcctggg cgcctctgtg ctgggcctgg acgatatcca
5221 cagggcctgg cgcaccttcg tgctgcgtgt gcgggcccag gacccgccgc ctgagctgta
5281 ctttgtcaag gtggatgtga cgggcgcgta cgacaccatc ccccaggaca ggctcacgga
5341 ggtcatcgcc agcatcatca aaccccagaa cacgtactgc gtgcgtcggt atgccgtggt
5401 ccagaaggcc gcccatgggc acgtccgcaa ggccttcaag agccacgtct ctaccttgac
5461 agacctccag ccgtacatgc gacagttcgt ggctcacctg caggagacca gcccgctgag
5521 ggatgccgtc gtcatcgagc agagctcctc cctgaatgag gccagcagtg gcctcttcga
5581 cgtcttccta cgcttcatgt gccaccacgc cgtgcgcatc aggggcaagt cctacgtcca
5641 gtgccagggg atcccgcagg gctccatcct ctccacgctg ctctgcagcc tgtgctacgg
5701 cgacatggag aacaagctgt ttgcggggat tcggcgggac gggctgctcc tgcgtttggt
5761 ggatgatttc ttgttggtga cacctcacct cacccacgcg aaaaccttcc tcaggaccct
5821 ggtccgaggt gtccctgagt atggctgcgt ggtgaacttg cggaagacag tggtgaactt
5881 ccctgtagaa gacgaggccc tgggtggcac ggcttttgtt cagatgccgg cccacggcct
5941 attcccctgg tgcggcctgc tgctggatac ccggaccctg gaggtgcaga gcgactactc
6001 cagctatgcc cggacctcca tcagagccag tctcaccttc aaccgcggct tcaaggctgg
6061 gaggaacatg cgtcgcaaac tctttggggt cttgcggctg aagtgtcaca gcctgtttct
6121 ggatttgcag gtgaacagcc tccagacggt gtgcaccaac atctacaaga tcctcctgct
6181 gcaggcgtac aggtttcacg catgtgtgct gcagctccca tttcatcagc aagtttggaa
6241 gaaccccaca tttttcctgc gcgtcatctc tgacacggcc tccctctgct actccatcct
6301 gaaagccaag aacgcaggga tgtcgctggg ggccaagggc gccgccggcc ctctgccctc
6361 cgaggccgtg cagtggctgt gccaccaagc attcctgctc aagctgactc gacaccgtgt
6421 cacctacgtg ccactcctgg ggtcactcag gacagcccag acgcagctga gtcggaagct
6481 cccggggacg acgctgactg ccctggaggc cgcagccaac ccggcactgc cctcagactt
6541 caagaccatc ctggactgat ggccacccgc ccacagccag gccgagagca gacaccagca
6601 gccctgtcac gccgggctct acgtcccagg gagggagggg cggcccacac ccaggcccgc
6661 accgctggga gtctgaggcc tgagtgagtg tttggccgag gcctgcatgt ccggctgaag
6721 gctgagtgtc cggctgaggc ctgagcgagt gtccagccaa gggctgagtg tccagcacac
6781 ctgccgtctt cacttcccca caggctggcg ctcggcttag gctcgagcat catcaccatc
6841 accattgatg atccggctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
6901 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
6961 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
7021 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
7081 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
7141 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagtgacca gatctaatgc
7201 gttctaatgc gtaccagatc taatgcgtgc tagcgaccag atctaatgcg tgctaggccc
7261 ttttgcaaaa agcttgggct gcaggtcgag gcggatctga tcaagagaca ggatgaggat
7321 cgtttcgcat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
7381 aggctttttt ggaggccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
7441 acgtccctcg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
7501 acaccgtgga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
7561 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
7621 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
7681 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggg ctcctggcgc
7741 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
7801 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
7861 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
7921 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
7981 gcaagcccgg tgcctgaacg cgttccggaa atcaacctct ggattacaaa atttgtgaaa
8041 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
8101 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
8161 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
8221 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
8281 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
8341 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
8401 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
8461 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
8521 tgccggctct gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt cggatctccc
8581 tttggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
8641 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
8701 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
8761 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
8821 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
8881 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
8941 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gggcctgacc agatctaatg
9001 cgtaggccgt ttaaaccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
9061 tgttgcccgg gcgcgatcgc tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
9121 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
9181 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
9241 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagcagat
9301 cgatctgcat ctatgtcggg tgcggagaaa gaggtaatga aatggcatta tgggtattat
9361 gggtctgcat taatgaatcg gccaacgatc ccggtgtgaa ataccgcaca gatgcgtaag
9421 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
9481 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
9541 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
9601 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
9661 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
9721 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
9781 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
9841 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
9901 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
9961 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
10021 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
10081 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
10141 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
10201 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
10261 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
10321 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
10381 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
10441 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
10501 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
10561 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
10621 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
10681 caacgttgtt gaaaaaggat cttcacctag atccttttca cgtagaaagc cagtccgcag
10741 aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca
10801 agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg
10861 gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg
10921 aagccctgca aagtaaactg gatggctttc tcgccgccaa ggatctgatg gcgcagggga
10981 tcaagctctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
11041 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
11101 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
11161 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta
11221 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
11281 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
11341 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
11401 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
11461 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
11521 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc
11581 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
11641 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
11701 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
11761 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaattttg
11821 ttaaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaacatccc
11881 ttataaatca aaagaataga ccgcgatagg gttgagtgtt gttccagttt ggaacaagag
11941 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
12001 tggcccacta cgtgaaccat cacccaaatc aagttttttg cggtcgaggt gccgtaaagc
12061 tctaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
12121 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
12181 agcggtcacg ctgcgcgtaa ccaccacacc cgcgcgctta atgcgccgct acagggcgcg
12241 tccattcgcc attcaggatc gaattaattc ttaattaaca tcatcaataa tatacctt
                            LOCUS       dna                     12298 bp                                   
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
Other Gene 880..1060
/gene="HIV-1_5_LTR other"
Other Gene 1171..1215
/gene="HIV-1_psi_pack other"
misc_binding 1257..1262
/dbxref="REBASE:NruI"
Regulatory_Seq 1723..1956
/gene="RRE reg"
ORF 1766..2449
/sequence="ORF_2 rf(2)"
misc_binding 2636..2641
/dbxref="REBASE:NdeI"
Promoter 2911..2992
/gene="CMV prom"
Regulatory_Seq 3048..3087
/gene="tetO reg"
ORF 3089..3775
/sequence="ORF_7 rf(4)"
misc_binding 3151..3156
/dbxref="REBASE:EcoRI"
ORF 3161..6559
/sequence="ORF_3 rf(2)"
ORF 4196..5416
/sequence="ORF_6 rf(4)"
misc_binding 5213..5218
/dbxref="REBASE:EcoRV"
misc_binding 5649..5654
/dbxref="REBASE:BamHI"
Tag 6828..6845
/gene="6xHis tag"
Regulatory_Seq 6856..7092
/gene="SV40ER reg"
Promoter 6879..7147
/gene="SV40 prom"
Rep_Origin 7046..7123
/gene="SV40 origin"
ORF 7329..7997
/sequence="ORF_4 rf(3)"
Marker 7398..7997
/gene="puro marker"
ORF 8100..8750
/sequence="ORF_5 rf(3)"
Other Gene 8671..8723
/gene="delta_U3 other"
Other Gene 8724..8904
/gene="HIV-1_5_LTR other"
Rep_Origin 9610..10229
/gene="pBR322 origin"
Promoter 10882..10931
/gene="NEOKAN prom"
ORF 11020..11814
/sequence="ORF_1 rf(1)"
Marker 11023..11811
/gene="NTP_II marker"
Rep_Origin 11919..12224
/gene="f1 origin"
BASE COUNT 2715 a 3389 c 3549 g 2645 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc
541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt aatgtagtct
661 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
721 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
781 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
841 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
901 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
961 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
1021 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
1081 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
1141 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
1201 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
1261 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
1321 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
1381 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
1441 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
1501 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
1561 cacagcaagc gccgctgatc ttcagacctg gaggaggaga tatgagggac aattgagaag
1621 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1681 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1741 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1801 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1861 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1921 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1981 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
2041 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
2101 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
2161 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
2221 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
2281 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
2341 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
2401 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgataag cttgggagtt
2461 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
2521 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
2581 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
2641 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
2701 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
2761 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
2821 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
2881 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
2941 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
3001 acgccatcca cgctgttttg acctccatag aagaaccgag tttaaactcc ctatcagtga
3061 tagagatctc cctatcagtg atagagagct agccccggga tcgatcaatt gagtacttac
3121 gtaggtaccc cagtgtggtg gcctgcaggt gaattccacc atgccgcgcg ctccccgctg
3181 ccgagccgtg cgctccctgc tgcgcagcca ctaccgcgag gtgctgccgc tggccacgtt
3241 cgtgcggcgc ctggggcccc agggctggcg gctggtgcag cgcggggacc cggcggcttt
3301 ccgcgcgctg gtggcccagt gcctggtgtg cgtgccctgg gacgcacggc cgccccccgc
3361 cgccccctcc ttccgccagg tgtcctgcct gaaggagctg gtggcccgag tgctgcagag
3421 gctgtgcgag cgcggcgcga agaacgtgct ggccttcggc ttcgcgctgc tggacggggc
3481 ccgcgggggc ccccccgagg ccttcaccac cagcgtgcgc agctacctgc ccaacacggt
3541 gaccgacgca ctgcggggga gcggggcgtg ggggctgctg ctgcgccgcg tgggcgacga
3601 cgtgctggtt cacctgctgg cacgctgcgc gctctttgtg ctggtggctc ccagctgcgc
3661 ctaccaggtg tgcgggccgc cgctgtacca gctcggcgct gccactcagg cccggccccc
3721 gccacacgct agtggacccc gaaggcgtct gggatgcgaa cgggcctgga accatagcgt
3781 cagggaggcc ggggtccccc tgggcctgcc agccccgggt gcgaggaggc gcgggggcag
3841 tgccagccga agtctgccgt tgcccaagag gcccaggcgt ggcgctgccc ctgagccgga
3901 gcggacgccc gttgggcagg ggtcctgggc ccacccgggc aggacgcgtg gaccgagtga
3961 ccgtggtttc tgtgtggtgt cacctgccag acccgccgaa gaagccacct ctttggaggg
4021 tgcgctctct ggcacgcgcc actcccaccc atccgtgggc cgccagcacc acgcgggccc
4081 cccatccaca tcgcggccac cacgtccctg ggacacgcct tgtcccccgg tgtacgccga
4141 gaccaagcac ttcctctact cctcaggcga caaggagcag ctgcggccct ccttcctact
4201 cagctctctg aggcccagcc tgactggcgc tcggaggctc gtggagacca tctttctggg
4261 ttccaggccc tggatgccag ggactccccg caggttgccc cgcctgcccc agcgctactg
4321 gcaaatgcgg cccctgtttc tggagctgct tgggaaccac gcgcagtgcc cctacggggt
4381 gctcctcaag acgcactgcc cgctgcgagc tgcggtcacc ccagcagccg gtgtctgtgc
4441 ccgggagaag ccccagggct ctgtggcggc ccccgaggag gaggacacag acccccgtcg
4501 cctggtgcag ctgctccgcc agcacagcag cccctggcag gtgtacggct tcgtgcgggc
4561 ctgcctgcgc cggctggtgc ccccaggcct ctggggctcc aggcacaacg aacgccgctt
4621 cctcaggaac accaagaagt tcatctccct ggggaagcat gccaagctct cgctgcagga
4681 gctgacgtgg aagatgagcg tgcgggactg cgcttggctg cgcaggagcc caggggttgg
4741 ctgtgttccg gccgcagagc accgtctgcg tgaggagatc ctggccaagt tcctgcactg
4801 gctgatgagt gtgtacgtcg tcgagctgct caggtctttc ttttatgtca cggagaccac
4861 gtttcaaaag aacaggctct ttttctaccg gaagagtgtc tggagcaagt tgcaaagcat
4921 tggaatcaga cagcacttga agagggtgca gctgcgggag ctgtcggaag cagaggtcag
4981 gcagcatcgg gaagccaggc ccgccctgct gacgtccaga ctccgcttca tccccaagcc
5041 tgacgggctg cggccgattg tgaacatgga ctacgtcgtg ggagccagaa cgttccgcag
5101 agaaaagagg gccgagcgtc tcacctcgag ggtgaaggca ctgttcagcg tgctcaacta
5161 cgagcgggcg cggcgccccg gcctcctggg cgcctctgtg ctgggcctgg acgatatcca
5221 cagggcctgg cgcaccttcg tgctgcgtgt gcgggcccag gacccgccgc ctgagctgta
5281 ctttgtcaag gtggatgtga cgggcgcgta cgacaccatc ccccaggaca ggctcacgga
5341 ggtcatcgcc agcatcatca aaccccagaa cacgtactgc gtgcgtcggt atgccgtggt
5401 ccagaaggcc gcccatgggc acgtccgcaa ggccttcaag agccacgtct ctaccttgac
5461 agacctccag ccgtacatgc gacagttcgt ggctcacctg caggagacca gcccgctgag
5521 ggatgccgtc gtcatcgagc agagctcctc cctgaatgag gccagcagtg gcctcttcga
5581 cgtcttccta cgcttcatgt gccaccacgc cgtgcgcatc aggggcaagt cctacgtcca
5641 gtgccagggg atcccgcagg gctccatcct ctccacgctg ctctgcagcc tgtgctacgg
5701 cgacatggag aacaagctgt ttgcggggat tcggcgggac gggctgctcc tgcgtttggt
5761 ggatgatttc ttgttggtga cacctcacct cacccacgcg aaaaccttcc tcaggaccct
5821 ggtccgaggt gtccctgagt atggctgcgt ggtgaacttg cggaagacag tggtgaactt
5881 ccctgtagaa gacgaggccc tgggtggcac ggcttttgtt cagatgccgg cccacggcct
5941 attcccctgg tgcggcctgc tgctggatac ccggaccctg gaggtgcaga gcgactactc
6001 cagctatgcc cggacctcca tcagagccag tctcaccttc aaccgcggct tcaaggctgg
6061 gaggaacatg cgtcgcaaac tctttggggt cttgcggctg aagtgtcaca gcctgtttct
6121 ggatttgcag gtgaacagcc tccagacggt gtgcaccaac atctacaaga tcctcctgct
6181 gcaggcgtac aggtttcacg catgtgtgct gcagctccca tttcatcagc aagtttggaa
6241 gaaccccaca tttttcctgc gcgtcatctc tgacacggcc tccctctgct actccatcct
6301 gaaagccaag aacgcaggga tgtcgctggg ggccaagggc gccgccggcc ctctgccctc
6361 cgaggccgtg cagtggctgt gccaccaagc attcctgctc aagctgactc gacaccgtgt
6421 cacctacgtg ccactcctgg ggtcactcag gacagcccag acgcagctga gtcggaagct
6481 cccggggacg acgctgactg ccctggaggc cgcagccaac ccggcactgc cctcagactt
6541 caagaccatc ctggactgat ggccacccgc ccacagccag gccgagagca gacaccagca
6601 gccctgtcac gccgggctct acgtcccagg gagggagggg cggcccacac ccaggcccgc
6661 accgctggga gtctgaggcc tgagtgagtg tttggccgag gcctgcatgt ccggctgaag
6721 gctgagtgtc cggctgaggc ctgagcgagt gtccagccaa gggctgagtg tccagcacac
6781 ctgccgtctt cacttcccca caggctggcg ctcggcttag gctcgagcat catcaccatc
6841 accattgatg atccggctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
6901 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
6961 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
7021 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
7081 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
7141 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagtgacca gatctaatgc
7201 gttctaatgc gtaccagatc taatgcgtgc tagcgaccag atctaatgcg tgctaggccc
7261 ttttgcaaaa agcttgggct gcaggtcgag gcggatctga tcaagagaca ggatgaggat
7321 cgtttcgcat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
7381 aggctttttt ggaggccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
7441 acgtccctcg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
7501 acaccgtgga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
7561 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
7621 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
7681 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggg ctcctggcgc
7741 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
7801 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
7861 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
7921 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
7981 gcaagcccgg tgcctgaacg cgttccggaa atcaacctct ggattacaaa atttgtgaaa
8041 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
8101 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
8161 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
8221 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
8281 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
8341 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
8401 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
8461 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
8521 tgccggctct gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt cggatctccc
8581 tttggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
8641 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
8701 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
8761 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
8821 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
8881 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
8941 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gggcctgacc agatctaatg
9001 cgtaggccgt ttaaaccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
9061 tgttgcccgg gcgcgatcgc tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
9121 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
9181 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
9241 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagcagat
9301 cgatctgcat ctatgtcggg tgcggagaaa gaggtaatga aatggcatta tgggtattat
9361 gggtctgcat taatgaatcg gccaacgatc ccggtgtgaa ataccgcaca gatgcgtaag
9421 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
9481 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
9541 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
9601 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
9661 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
9721 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
9781 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
9841 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
9901 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
9961 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
10021 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
10081 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
10141 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
10201 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
10261 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
10321 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
10381 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
10441 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
10501 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
10561 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
10621 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
10681 caacgttgtt gaaaaaggat cttcacctag atccttttca cgtagaaagc cagtccgcag
10741 aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca
10801 agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg
10861 gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg
10921 aagccctgca aagtaaactg gatggctttc tcgccgccaa ggatctgatg gcgcagggga
10981 tcaagctctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
11041 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
11101 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
11161 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta
11221 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
11281 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
11341 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
11401 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
11461 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
11521 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc
11581 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
11641 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
11701 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
11761 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaattttg
11821 ttaaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaacatccc
11881 ttataaatca aaagaataga ccgcgatagg gttgagtgtt gttccagttt ggaacaagag
11941 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
12001 tggcccacta cgtgaaccat cacccaaatc aagttttttg cggtcgaggt gccgtaaagc
12061 tctaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
12121 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
12181 agcggtcacg ctgcgcgtaa ccaccacacc cgcgcgctta atgcgccgct acagggcgcg
12241 tccattcgcc attcaggatc gaattaattc ttaattaaca tcatcaataa tatacctt
//