pLenti-hTERT-2A-CDK4-Puro
Vector Name | pLenti-hTERT-2A-CDK4-Puro |
VectorType | Lentiviral Plasmid |
Antibiotic Information | Bacterial: Kanamycin. Mammalian: Puromycin. |
Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
Additional Information | The gene expression is driven by a CMV promoter, without any Tag or reporter. Vector with Insert: 4422bp Full plasmid size: 12510bp |
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***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcGAT ATCAACAAGT TTGTACAAAA AAGTTGGCat gccgcgcgct ccccgctgcc 3121 gagccgtgcg ctccctgctg cgcagccact accgcgaggt gctgccgctg gccacgttcg 3181 tgcggcgcct ggggccccag ggctggcggc tggtgcagcg cggggacccg gcggctttcc 3241 gcgcgctggt ggcccagtgc ctggtgtgcg tgccctggga cgcacggccg ccccccgccg 3301 ccccctcctt ccgccaggtg tcctgcctga aggagctggt ggcccgagtg ctgcagaggc 3361 tgtgcgagcg cggcgcgaag aacgtgctgg ccttcggctt cgcgctgctg gacggggccc 3421 gcgggggccc ccccgaggcc ttcaccacca gcgtgcgcag ctacctgccc aacacggtga 3481 ccgacgcact gcgggggagc ggggcgtggg ggctgctgct gcgccgcgtg ggcgacgacg 3541 tgctggttca cctgctggca cgctgcgcgc tctttgtgct ggtggctccc agctgcgcct 3601 accaggtgtg cgggccgccg ctgtaccagc tcggcgctgc cactcaggcc cggcccccgc 3661 cacacgctag tggaccccga aggcgtctgg gatgcgaacg ggcctggaac catagcgtca 3721 gggaggccgg ggtccccctg ggcctgccag ccccgggtgc gaggaggcgc gggggcagtg 3781 ccagccgaag tctgccgttg cccaagaggc ccaggcgtgg cgctgcccct gagccggagc 3841 ggacgcccgt tgggcagggg tcctgggccc acccgggcag gacgcgtgga ccgagtgacc 3901 gtggtttctg tgtggtgtca cctgccagac ccgccgaaga agccacctct ttggagggtg 3961 cgctctctgg cacgcgccac tcccacccat ccgtgggccg ccagcaccac gcgggccccc 4021 catccacatc gcggccacca cgtccctggg acacgccttg tcccccggtg tacgccgaga 4081 ccaagcactt cctctactcc tcaggcgaca aggagcagct gcggccctcc ttcctactca 4141 gctctctgag gcccagcctg actggcgctc ggaggctcgt ggagaccatc tttctgggtt 4201 ccaggccctg gatgccaggg actccccgca ggttgccccg cctgccccag cgctactggc 4261 aaatgcggcc cctgtttctg gagctgcttg ggaaccacgc gcagtgcccc tacggggtgc 4321 tcctcaagac gcactgcccg ctgcgagctg cggtcacccc agcagccggt gtctgtgccc 4381 gggagaagcc ccagggctct gtggcggccc ccgaggagga ggacacagac ccccgtcgcc 4441 tggtgcagct gctccgccag cacagcagcc cctggcaggt gtacggcttc gtgcgggcct 4501 gcctgcgccg gctggtgccc ccaggcctct ggggctccag gcacaacgaa cgccgcttcc 4561 tcaggaacac caagaagttc atctccctgg ggaagcatgc caagctctcg ctgcaggagc 4621 tgacgtggaa gatgagcgtg cgggactgcg cttggctgcg caggagccca ggggttggct 4681 gtgttccggc cgcagagcac cgtctgcgtg aggagatcct ggccaagttc ctgcactggc 4741 tgatgagtgt gtacgtcgtc gagctgctca ggtctttctt ttatgtcacg gagaccacgt 4801 ttcaaaagaa caggctcttt ttctaccgga agagtgtctg gagcaagttg caaagcattg 4861 gaatcagaca gcacttgaag agggtgcagc tgcgggagct gtcggaagca gaggtcaggc 4921 agcatcggga agccaggccc gccctgctga cgtccagact ccgcttcatc cccaagcctg 4981 acgggctgcg gccgattgtg aacatggact acgtcgtggg agccagaacg ttccgcagag 5041 aaaagagggc cgagcgtctc acctcgaggg tgaaggcact gttcagcgtg ctcaactacg 5101 agcgggcgcg gcgccccggc ctcctgggcg cctctgtgct gggcctggac gatatccaca 5161 gggcctggcg caccttcgtg ctgcgtgtgc gggcccagga cccgccgcct gagctgtact 5221 ttgtcaaggt ggatgtgacg ggcgcgtacg acaccatccc ccaggacagg ctcacggagg 5281 tcatcgccag catcatcaaa ccccagaaca cgtactgcgt gcgtcggtat gccgtggtcc 5341 agaaggccgc ccatgggcac gtccgcaagg ccttcaagag ccacgtctct accttgacag 5401 acctccagcc gtacatgcga cagttcgtgg ctcacctgca ggagaccagc ccgctgaggg 5461 atgccgtcgt catcgagcag agctcctccc tgaatgaggc cagcagtggc ctcttcgacg 5521 tcttcctacg cttcatgtgc caccacgccg tgcgcatcag gggcaagtcc tacgtccagt 5581 gccaggggat cccgcagggc tccatcctct ccacgctgct ctgcagcctg tgctacggcg 5641 acatggagaa caagctgttt gcggggattc ggcgggacgg gctgctcctg cgtttggtgg 5701 atgatttctt gttggtgaca cctcacctca cccacgcgaa aaccttcctc aggaccctgg 5761 tccgaggtgt ccctgagtat ggctgcgtgg tgaacttgcg gaagacagtg gtgaacttcc 5821 ctgtagaaga cgaggccctg ggtggcacgg cttttgttca gatgccggcc cacggcctat 5881 tcccctggtg cggcctgctg ctggataccc ggaccctgga ggtgcagagc gactactcca 5941 gctatgcccg gacctccatc agagccagtc tcaccttcaa ccgcggcttc aaggctggga 6001 ggaacatgcg tcgcaaactc tttggggtct tgcggctgaa gtgtcacagc ctgtttctgg 6061 atttgcaggt gaacagcctc cagacggtgt gcaccaacat ctacaagatc ctcctgctgc 6121 aggcgtacag gtttcacgca tgtgtgctgc agctcccatt tcatcagcaa gtttggaaga 6181 accccacatt tttcctgcgc gtcatctctg acacggcctc cctctgctac tccatcctga 6241 aagccaagaa cgcagggatg tcgctggggg ccaagggcgc cgccggccct ctgccctccg 6301 aggccgtgca gtggctgtgc caccaagcat tcctgctcaa gctgactcga caccgtgtca 6361 cctacgtgcc actcctgggg tcactcagga cagcccagac gcagctgagt cggaagctcc 6421 cggggacgac gctgactgcc ctggaggccg cagccaaccc ggcactgccc tcagacttca 6481 agaccatcct ggactgCCCA ACTTTCTTGT ACAAAGTGGT Tgatatcggg cccgcggccg 6541 ctggatcctc tagcgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc 6601 ccggccctAT GGCTACCTCT CGATATGAGC CAGTGGCTGA AATTGGTGTC GGTGCCTATG 6661 GGACAGTGTA CAAGGCCCGT GATCCCCACA GTGGCCACTT TGTGGCCCTC AAGAGTGTGA 6721 GAGTCCCCAA TGGAGGAGGA GGTGGAGGAG GCCTTCCCAT CAGCACAGTT CGTGAGGTGG 6781 CTTTACTGAG GCGACTGGAG GCTTTTGAGC ATCCCAATGT TGTCCGGCTG ATGGACGTCT 6841 GTGCCACATC CCGAACTGAC CGGGAGATCA AGGTAACCCT GGTGTTTGAG CATGTAGACC 6901 AGGACCTAAG GACATATCTG GACAAGGCAC CCCCACCAGG CTTGCCAGCC GAAACGATCA 6961 AGGATCTGAT GCGCCAGTTT CTAAGAGGCC TAGATTTCCT TCATGCCAAT TGCATCGTTC 7021 ACCGAGATCT GAAGCCAGAG AACATTCTGG TGACAAGTGG TGGAACAGTC AAGCTGGCTG 7081 ACTTTGGCCT GGCCAGAATC TACAGCTACC AGATGGCACT TACACCCGTG GTTGTTACAC 7141 TCTGGTACCG AGCTCCCGAA GTTCTTCTGC AGTCCACATA TGCAACACCT GTGGACATGT 7201 GGAGTGTTGG CTGTATCTTT GCAGAGATGT TTCGTCGAAA GCCTCTCTTC TGTGGAAACT 7261 CTGAAGCCGA CCAGTTGGGC AAAATCTTTG ACCTGATTGG GCTGCCTCCA GAGGATGACT 7321 GGCCTCGAGA TGTATCCCTG CCCCGTGGAG CCTTTCCCCC CAGAGGGCCC CGCCCAGTGC 7381 AGTCGGTGGT ACCTGAGATG GAGGAGTCGG GAGCACAGCT GCTGCTGGAA ATGCTGACTT 7441 TTAACCCACA CAAGCGAATC TCTGCCTTTC GAGCTCTGCA GCACTCTTAT CTACATAAGG 7501 ATGAAGGTAA TCCGGAGTGA ctcgagtacc catacgacgt cccagactac gcttgagttt 7561 aaacacgcgt ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 7621 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt 7681 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 7741 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 7801 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 7861 ttttttggag gccatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt 7921 ccctcgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac 7981 cgtggacccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg 8041 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg 8101 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc 8161 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaagggctcc tggcgccgca 8221 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg 8281 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt 8341 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt 8401 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa 8461 gcccggtgcc tgaacgcgtt ccggaaatca acctctggat tacaaaattt gtgaaagatt 8521 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc 8581 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg 8641 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac 8701 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc 8761 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc 8821 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa 8881 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc 8941 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc 9001 ggctctgcgg cctcttccgc gtctcgcctt cgccctcaga cgagtcggat ctccctttgg 9061 gccgcctccc cgcctgtccg gatggaaggg ctaattcact cccaacgaat acaagatctg 9121 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc 9181 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg 9241 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg 9301 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca 9361 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa 9421 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 9481 ggtttgtcca aactcatcaa tgtatcttat catgtctggc atctatgtcg ggtgcggaga 9541 aagaggtaat gaaatggcat tatgggtatt atgggtctgc attaatgaat cggccaacga 9601 tcccggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc 9661 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 9721 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 9781 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 9841 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 9901 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 9961 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 10021 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 10081 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 10141 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 10201 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 10261 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 10321 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 10381 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 10441 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 10501 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 10561 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 10621 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 10681 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 10741 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 10801 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 10861 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgaaaaagg atcttcacct 10921 agatcctttt cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca 10981 gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca 11041 gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat 11101 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt 11161 tctcgccgcc aaggatctga tggcgcaggg gatcaagctc tgatcaagag acaggatgag 11221 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg 11281 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt 11341 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc 11401 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt 11461 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag 11521 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg 11581 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag 11641 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg 11701 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga 11761 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca 11821 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc 11881 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg 11941 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct 12001 atcgccttct tgacgagttc ttctgaattt tgttaaaatt tttgttaaat cagctcattt 12061 tttaaccaat aggccgaaat cggcaacatc ccttataaat caaaagaata gaccgcgata 12121 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac 12181 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcacccaaa 12241 tcaagttttt tgcggtcgag gtgccgtaaa gctctaaatc ggaaccctaa agggagcccc 12301 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg 12361 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca 12421 cccgcgcgct taatgcgccg ctacagggcg cgtccattcg ccattcagga tcgaattaat 12481 tcttaattaa catcatcaat aatatacctt
LOCUS dna 12510 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 457..462 /dbxref="REBASE:HincII" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" misc_binding 1092..1097 /dbxref="REBASE:NruI" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" Promoter 2885..2966 /gene="CMV prom" ORF 2901..7520 /sequence="ORF_3 rf(3)" Regulatory_Seq 3022..3061 /gene="tetO reg" ORF 3072..3713 /sequence="ORF_6 rf(5)" ORF 4134..5354 /sequence="ORF_5 rf(5)" misc_binding 6534..6541 /dbxref="REBASE:NotI" Promoter 7571..7839 /gene="SV40 prom" Rep_Origin 7738..7815 /gene="SV40 origin" ORF 7805..8473 /sequence="ORF_2 rf(2)" misc_binding 7818..7830 /dbxref="REBASE:SfiI" Marker 7874..8473 /gene="puro marker" Other Gene 9083..9135 /gene="delta_U3 other" Other Gene 9136..9316 /gene="HIV-1_5_LTR other" Rep_Origin 9822..10441 /gene="pBR322 origin" Promoter 11094..11143 /gene="NEOKAN prom" misc_binding 11201..11206 /dbxref="REBASE:BclI" ORF 11232..12026 /sequence="ORF_4 rf(3)" Marker 11235..12023 /gene="NTP_II marker" Rep_Origin 12131..12436 /gene="f1 origin" BASE COUNT 2823 a 3408 c 3559 g 2720 t 0 others ORIGIN 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcat gccgcgcgct ccccgctgcc 3121 gagccgtgcg ctccctgctg cgcagccact accgcgaggt gctgccgctg gccacgttcg 3181 tgcggcgcct ggggccccag ggctggcggc tggtgcagcg cggggacccg gcggctttcc 3241 gcgcgctggt ggcccagtgc ctggtgtgcg tgccctggga cgcacggccg ccccccgccg 3301 ccccctcctt ccgccaggtg tcctgcctga aggagctggt ggcccgagtg ctgcagaggc 3361 tgtgcgagcg cggcgcgaag aacgtgctgg ccttcggctt cgcgctgctg gacggggccc 3421 gcgggggccc ccccgaggcc ttcaccacca gcgtgcgcag ctacctgccc aacacggtga 3481 ccgacgcact gcgggggagc ggggcgtggg ggctgctgct gcgccgcgtg ggcgacgacg 3541 tgctggttca cctgctggca cgctgcgcgc tctttgtgct ggtggctccc agctgcgcct 3601 accaggtgtg cgggccgccg ctgtaccagc tcggcgctgc cactcaggcc cggcccccgc 3661 cacacgctag tggaccccga aggcgtctgg gatgcgaacg ggcctggaac catagcgtca 3721 gggaggccgg ggtccccctg ggcctgccag ccccgggtgc gaggaggcgc gggggcagtg 3781 ccagccgaag tctgccgttg cccaagaggc ccaggcgtgg cgctgcccct gagccggagc 3841 ggacgcccgt tgggcagggg tcctgggccc acccgggcag gacgcgtgga ccgagtgacc 3901 gtggtttctg tgtggtgtca cctgccagac ccgccgaaga agccacctct ttggagggtg 3961 cgctctctgg cacgcgccac tcccacccat ccgtgggccg ccagcaccac gcgggccccc 4021 catccacatc gcggccacca cgtccctggg acacgccttg tcccccggtg tacgccgaga 4081 ccaagcactt cctctactcc tcaggcgaca aggagcagct gcggccctcc ttcctactca 4141 gctctctgag gcccagcctg actggcgctc ggaggctcgt ggagaccatc tttctgggtt 4201 ccaggccctg gatgccaggg actccccgca ggttgccccg cctgccccag cgctactggc 4261 aaatgcggcc cctgtttctg gagctgcttg ggaaccacgc gcagtgcccc tacggggtgc 4321 tcctcaagac gcactgcccg ctgcgagctg cggtcacccc agcagccggt gtctgtgccc 4381 gggagaagcc ccagggctct gtggcggccc ccgaggagga ggacacagac ccccgtcgcc 4441 tggtgcagct gctccgccag cacagcagcc cctggcaggt gtacggcttc gtgcgggcct 4501 gcctgcgccg gctggtgccc ccaggcctct ggggctccag gcacaacgaa cgccgcttcc 4561 tcaggaacac caagaagttc atctccctgg ggaagcatgc caagctctcg ctgcaggagc 4621 tgacgtggaa gatgagcgtg cgggactgcg cttggctgcg caggagccca ggggttggct 4681 gtgttccggc cgcagagcac cgtctgcgtg aggagatcct ggccaagttc ctgcactggc 4741 tgatgagtgt gtacgtcgtc gagctgctca ggtctttctt ttatgtcacg gagaccacgt 4801 ttcaaaagaa caggctcttt ttctaccgga agagtgtctg gagcaagttg caaagcattg 4861 gaatcagaca gcacttgaag agggtgcagc tgcgggagct gtcggaagca gaggtcaggc 4921 agcatcggga agccaggccc gccctgctga cgtccagact ccgcttcatc cccaagcctg 4981 acgggctgcg gccgattgtg aacatggact acgtcgtggg agccagaacg ttccgcagag 5041 aaaagagggc cgagcgtctc acctcgaggg tgaaggcact gttcagcgtg ctcaactacg 5101 agcgggcgcg gcgccccggc ctcctgggcg cctctgtgct gggcctggac gatatccaca 5161 gggcctggcg caccttcgtg ctgcgtgtgc gggcccagga cccgccgcct gagctgtact 5221 ttgtcaaggt ggatgtgacg ggcgcgtacg acaccatccc ccaggacagg ctcacggagg 5281 tcatcgccag catcatcaaa ccccagaaca cgtactgcgt gcgtcggtat gccgtggtcc 5341 agaaggccgc ccatgggcac gtccgcaagg ccttcaagag ccacgtctct accttgacag 5401 acctccagcc gtacatgcga cagttcgtgg ctcacctgca ggagaccagc ccgctgaggg 5461 atgccgtcgt catcgagcag agctcctccc tgaatgaggc cagcagtggc ctcttcgacg 5521 tcttcctacg cttcatgtgc caccacgccg tgcgcatcag gggcaagtcc tacgtccagt 5581 gccaggggat cccgcagggc tccatcctct ccacgctgct ctgcagcctg tgctacggcg 5641 acatggagaa caagctgttt gcggggattc ggcgggacgg gctgctcctg cgtttggtgg 5701 atgatttctt gttggtgaca cctcacctca cccacgcgaa aaccttcctc aggaccctgg 5761 tccgaggtgt ccctgagtat ggctgcgtgg tgaacttgcg gaagacagtg gtgaacttcc 5821 ctgtagaaga cgaggccctg ggtggcacgg cttttgttca gatgccggcc cacggcctat 5881 tcccctggtg cggcctgctg ctggataccc ggaccctgga ggtgcagagc gactactcca 5941 gctatgcccg gacctccatc agagccagtc tcaccttcaa ccgcggcttc aaggctggga 6001 ggaacatgcg tcgcaaactc tttggggtct tgcggctgaa gtgtcacagc ctgtttctgg 6061 atttgcaggt gaacagcctc cagacggtgt gcaccaacat ctacaagatc ctcctgctgc 6121 aggcgtacag gtttcacgca tgtgtgctgc agctcccatt tcatcagcaa gtttggaaga 6181 accccacatt tttcctgcgc gtcatctctg acacggcctc cctctgctac tccatcctga 6241 aagccaagaa cgcagggatg tcgctggggg ccaagggcgc cgccggccct ctgccctccg 6301 aggccgtgca gtggctgtgc caccaagcat tcctgctcaa gctgactcga caccgtgtca 6361 cctacgtgcc actcctgggg tcactcagga cagcccagac gcagctgagt cggaagctcc 6421 cggggacgac gctgactgcc ctggaggccg cagccaaccc ggcactgccc tcagacttca 6481 agaccatcct ggactgccca actttcttgt acaaagtggt tgatatcggg cccgcggccg 6541 ctggatcctc tagcgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc 6601 ccggccctat ggctacctct cgatatgagc cagtggctga aattggtgtc ggtgcctatg 6661 ggacagtgta caaggcccgt gatccccaca gtggccactt tgtggccctc aagagtgtga 6721 gagtccccaa tggaggagga ggtggaggag gccttcccat cagcacagtt cgtgaggtgg 6781 ctttactgag gcgactggag gcttttgagc atcccaatgt tgtccggctg atggacgtct 6841 gtgccacatc ccgaactgac cgggagatca aggtaaccct ggtgtttgag catgtagacc 6901 aggacctaag gacatatctg gacaaggcac ccccaccagg cttgccagcc gaaacgatca 6961 aggatctgat gcgccagttt ctaagaggcc tagatttcct tcatgccaat tgcatcgttc 7021 accgagatct gaagccagag aacattctgg tgacaagtgg tggaacagtc aagctggctg 7081 actttggcct ggccagaatc tacagctacc agatggcact tacacccgtg gttgttacac 7141 tctggtaccg agctcccgaa gttcttctgc agtccacata tgcaacacct gtggacatgt 7201 ggagtgttgg ctgtatcttt gcagagatgt ttcgtcgaaa gcctctcttc tgtggaaact 7261 ctgaagccga ccagttgggc aaaatctttg acctgattgg gctgcctcca gaggatgact 7321 ggcctcgaga tgtatccctg ccccgtggag cctttccccc cagagggccc cgcccagtgc 7381 agtcggtggt acctgagatg gaggagtcgg gagcacagct gctgctggaa atgctgactt 7441 ttaacccaca caagcgaatc tctgcctttc gagctctgca gcactcttat ctacataagg 7501 atgaaggtaa tccggagtga ctcgagtacc catacgacgt cccagactac gcttgagttt 7561 aaacacgcgt ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 7621 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt 7681 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 7741 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 7801 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 7861 ttttttggag gccatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt 7921 ccctcgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac 7981 cgtggacccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg 8041 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg 8101 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc 8161 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaagggctcc tggcgccgca 8221 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg 8281 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt 8341 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt 8401 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa 8461 gcccggtgcc tgaacgcgtt ccggaaatca acctctggat tacaaaattt gtgaaagatt 8521 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc 8581 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg 8641 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac 8701 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc 8761 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc 8821 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa 8881 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc 8941 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc 9001 ggctctgcgg cctcttccgc gtctcgcctt cgccctcaga cgagtcggat ctccctttgg 9061 gccgcctccc cgcctgtccg gatggaaggg ctaattcact cccaacgaat acaagatctg 9121 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc 9181 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg 9241 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg 9301 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca 9361 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa 9421 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 9481 ggtttgtcca aactcatcaa tgtatcttat catgtctggc atctatgtcg ggtgcggaga 9541 aagaggtaat gaaatggcat tatgggtatt atgggtctgc attaatgaat cggccaacga 9601 tcccggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc 9661 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 9721 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 9781 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 9841 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 9901 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 9961 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 10021 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 10081 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 10141 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 10201 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 10261 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 10321 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 10381 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 10441 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 10501 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 10561 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 10621 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 10681 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 10741 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 10801 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 10861 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgaaaaagg atcttcacct 10921 agatcctttt cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca 10981 gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca 11041 gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat 11101 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt 11161 tctcgccgcc aaggatctga tggcgcaggg gatcaagctc tgatcaagag acaggatgag 11221 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg 11281 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt 11341 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc 11401 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt 11461 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag 11521 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg 11581 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag 11641 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg 11701 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga 11761 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca 11821 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc 11881 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg 11941 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct 12001 atcgccttct tgacgagttc ttctgaattt tgttaaaatt tttgttaaat cagctcattt 12061 tttaaccaat aggccgaaat cggcaacatc ccttataaat caaaagaata gaccgcgata 12121 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac 12181 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcacccaaa 12241 tcaagttttt tgcggtcgag gtgccgtaaa gctctaaatc ggaaccctaa agggagcccc 12301 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg 12361 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca 12421 cccgcgcgct taatgcgccg ctacagggcg cgtccattcg ccattcagga tcgaattaat 12481 tcttaattaa catcatcaat aatatacctt //