pLenti-hTERT-No-Selection

                            LOCUS       dna                     10587 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    457..462
                     /dbxref="REBASE:HpaI"
     misc_binding    457..462
                     /dbxref="REBASE:HincII"
     Other Gene      715..895
                     /gene="HIV-1_5_LTR other"
     Other Gene      1006..1050
                     /gene="HIV-1_psi_pack other"
     misc_binding    1092..1097
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  1557..1790
                     /gene="RRE reg"
     ORF             1600..2301
                     /sequence="ORF_1 rf(1)"
     misc_binding    2611..2616
                     /dbxref="REBASE:NdeI"
     Promoter        2885..2966
                     /gene="CMV prom"
     Regulatory_Seq  3022..3061
                     /gene="tetO reg"
     ORF             3063..3749
                     /sequence="ORF_6 rf(5)"
     misc_binding    3074..3079
                     /dbxref="REBASE:ClaI"
     misc_binding    3098..3103
                     /dbxref="REBASE:KpnI"
     misc_binding    3125..3130
                     /dbxref="REBASE:EcoRI"
     ORF             3135..6533
                     /sequence="ORF_3 rf(3)"
     ORF             4170..5390
                     /sequence="ORF_5 rf(5)"
     misc_binding    5099..5104
                     /dbxref="REBASE:XhoI"
     misc_binding    5187..5192
                     /dbxref="REBASE:EcoRV"
     misc_binding    5623..5628
                     /dbxref="REBASE:BamHI"
     ORF             6533..7171
                     /sequence="ORF_2 rf(2)"
     misc_binding    6540..6547
                     /dbxref="REBASE:NotI"
     Other Gene      7160..7212
                     /gene="delta_U3 other"
     Other Gene      7213..7393
                     /gene="HIV-1_5_LTR other"
     Rep_Origin      7899..8518
                     /gene="pBR322 origin"
     Promoter        9171..9220
                     /gene="NEOKAN prom"
     misc_binding    9278..9283
                     /dbxref="REBASE:BclI"
     ORF             9309..10103
                     /sequence="ORF_4 rf(3)"
     Marker          9312..10100
                     /gene="NTP_II marker"
     Rep_Origin      10208..10513
                     /gene="f1 origin"
BASE COUNT    2430 a   2812 c   3002 g   2343 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta     
      481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag     
      541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg     
      601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact     
      661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct     
      721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt     
      781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac     
      841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc     
      901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc     
      961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa     
     1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg     
     1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata     
     1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc     
     1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga     
     1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc     
     1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca     
     1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag     
     1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta     
     1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga     
     1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg     
     1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg     
     1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc     
     1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg     
     1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat     
     1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac     
     1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat     
     1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca     
     2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga     
     2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg     
     2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa     
     2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga     
     2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag     
     2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa     
     2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat     
     2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt     
     2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa     
     2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc     
     2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct     
     2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt     
     2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg     
     2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca     
     2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca     
     2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc     
     3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag     
     3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accccagtgt ggtggcctgc     
     3121 aggtgaattc caccatgccg cgcgctcccc gctgccgagc cgtgcgctcc ctgctgcgca     
     3181 gccactaccg cgaggtgctg ccgctggcca cgttcgtgcg gcgcctgggg ccccagggct     
     3241 ggcggctggt gcagcgcggg gacccggcgg ctttccgcgc gctggtggcc cagtgcctgg     
     3301 tgtgcgtgcc ctgggacgca cggccgcccc ccgccgcccc ctccttccgc caggtgtcct     
     3361 gcctgaagga gctggtggcc cgagtgctgc agaggctgtg cgagcgcggc gcgaagaacg     
     3421 tgctggcctt cggcttcgcg ctgctggacg gggcccgcgg gggccccccc gaggccttca     
     3481 ccaccagcgt gcgcagctac ctgcccaaca cggtgaccga cgcactgcgg gggagcgggg     
     3541 cgtgggggct gctgctgcgc cgcgtgggcg acgacgtgct ggttcacctg ctggcacgct     
     3601 gcgcgctctt tgtgctggtg gctcccagct gcgcctacca ggtgtgcggg ccgccgctgt     
     3661 accagctcgg cgctgccact caggcccggc ccccgccaca cgctagtgga ccccgaaggc     
     3721 gtctgggatg cgaacgggcc tggaaccata gcgtcaggga ggccggggtc cccctgggcc     
     3781 tgccagcccc gggtgcgagg aggcgcgggg gcagtgccag ccgaagtctg ccgttgccca     
     3841 agaggcccag gcgtggcgct gcccctgagc cggagcggac gcccgttggg caggggtcct     
     3901 gggcccaccc gggcaggacg cgtggaccga gtgaccgtgg tttctgtgtg gtgtcacctg     
     3961 ccagacccgc cgaagaagcc acctctttgg agggtgcgct ctctggcacg cgccactccc     
     4021 acccatccgt gggccgccag caccacgcgg gccccccatc cacatcgcgg ccaccacgtc     
     4081 cctgggacac gccttgtccc ccggtgtacg ccgagaccaa gcacttcctc tactcctcag     
     4141 gcgacaagga gcagctgcgg ccctccttcc tactcagctc tctgaggccc agcctgactg     
     4201 gcgctcggag gctcgtggag accatctttc tgggttccag gccctggatg ccagggactc     
     4261 cccgcaggtt gccccgcctg ccccagcgct actggcaaat gcggcccctg tttctggagc     
     4321 tgcttgggaa ccacgcgcag tgcccctacg gggtgctcct caagacgcac tgcccgctgc     
     4381 gagctgcggt caccccagca gccggtgtct gtgcccggga gaagccccag ggctctgtgg     
     4441 cggcccccga ggaggaggac acagaccccc gtcgcctggt gcagctgctc cgccagcaca     
     4501 gcagcccctg gcaggtgtac ggcttcgtgc gggcctgcct gcgccggctg gtgcccccag     
     4561 gcctctgggg ctccaggcac aacgaacgcc gcttcctcag gaacaccaag aagttcatct     
     4621 ccctggggaa gcatgccaag ctctcgctgc aggagctgac gtggaagatg agcgtgcggg     
     4681 actgcgcttg gctgcgcagg agcccagggg ttggctgtgt tccggccgca gagcaccgtc     
     4741 tgcgtgagga gatcctggcc aagttcctgc actggctgat gagtgtgtac gtcgtcgagc     
     4801 tgctcaggtc tttcttttat gtcacggaga ccacgtttca aaagaacagg ctctttttct     
     4861 accggaagag tgtctggagc aagttgcaaa gcattggaat cagacagcac ttgaagaggg     
     4921 tgcagctgcg ggagctgtcg gaagcagagg tcaggcagca tcgggaagcc aggcccgccc     
     4981 tgctgacgtc cagactccgc ttcatcccca agcctgacgg gctgcggccg attgtgaaca     
     5041 tggactacgt cgtgggagcc agaacgttcc gcagagaaaa gagggccgag cgtctcacct     
     5101 cgagggtgaa ggcactgttc agcgtgctca actacgagcg ggcgcggcgc cccggcctcc     
     5161 tgggcgcctc tgtgctgggc ctggacgata tccacagggc ctggcgcacc ttcgtgctgc     
     5221 gtgtgcgggc ccaggacccg ccgcctgagc tgtactttgt caaggtggat gtgacgggcg     
     5281 cgtacgacac catcccccag gacaggctca cggaggtcat cgccagcatc atcaaacccc     
     5341 agaacacgta ctgcgtgcgt cggtatgccg tggtccagaa ggccgcccat gggcacgtcc     
     5401 gcaaggcctt caagagccac gtctctacct tgacagacct ccagccgtac atgcgacagt     
     5461 tcgtggctca cctgcaggag accagcccgc tgagggatgc cgtcgtcatc gagcagagct     
     5521 cctccctgaa tgaggccagc agtggcctct tcgacgtctt cctacgcttc atgtgccacc     
     5581 acgccgtgcg catcaggggc aagtcctacg tccagtgcca ggggatcccg cagggctcca     
     5641 tcctctccac gctgctctgc agcctgtgct acggcgacat ggagaacaag ctgtttgcgg     
     5701 ggattcggcg ggacgggctg ctcctgcgtt tggtggatga tttcttgttg gtgacacctc     
     5761 acctcaccca cgcgaaaacc ttcctcagga ccctggtccg aggtgtccct gagtatggct     
     5821 gcgtggtgaa cttgcggaag acagtggtga acttccctgt agaagacgag gccctgggtg     
     5881 gcacggcttt tgttcagatg ccggcccacg gcctattccc ctggtgcggc ctgctgctgg     
     5941 atacccggac cctggaggtg cagagcgact actccagcta tgcccggacc tccatcagag     
     6001 ccagtctcac cttcaaccgc ggcttcaagg ctgggaggaa catgcgtcgc aaactctttg     
     6061 gggtcttgcg gctgaagtgt cacagcctgt ttctggattt gcaggtgaac agcctccaga     
     6121 cggtgtgcac caacatctac aagatcctcc tgctgcaggc gtacaggttt cacgcatgtg     
     6181 tgctgcagct cccatttcat cagcaagttt ggaagaaccc cacatttttc ctgcgcgtca     
     6241 tctctgacac ggcctccctc tgctactcca tcctgaaagc caagaacgca gggatgtcgc     
     6301 tgggggccaa gggcgccgcc ggccctctgc cctccgaggc cgtgcagtgg ctgtgccacc     
     6361 aagcattcct gctcaagctg actcgacacc gtgtcaccta cgtgccactc ctggggtcac     
     6421 tcaggacagc ccagacgcag ctgagtcgga agctcccggg gacgacgctg actgccctgg     
     6481 aggccgcagc caacccggca ctgccctcag acttcaagac catcctggac tgatgggacg     
     6541 cggccgcatg actagttccg gaaatcaacc tctggattac aaaatttgtg aaagattgac     
     6601 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt     
     6661 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt     
     6721 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt     
     6781 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg     
     6841 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg     
     6901 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct     
     6961 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt     
     7021 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc     
     7081 tctgcggcct cttccgcgtc tcgccttcgc cctcagacga gtcggatctc cctttgggcc     
     7141 gcctccccgc ctgtccggat ggaagggcta attcactccc aacgaataca agatctgctt     
     7201 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa     
     7261 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt     
     7321 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg     
     7381 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag     
     7441 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa     
     7501 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt     
     7561 ttgtccaaac tcatcaatgt atcttatcat gtctggcatc tatgtcgggt gcggagaaag     
     7621 aggtaatgaa atggcattat gggtattatg ggtctgcatt aatgaatcgg ccaacgatcc     
     7681 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct     
     7741 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac     
     7801 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga     
     7861 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat     
     7921 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac     
     7981 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct     
     8041 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg     
     8101 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg     
     8161 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt     
     8221 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg     
     8281 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac     
     8341 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga     
     8401 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt     
     8461 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt     
     8521 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga     
     8581 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc     
     8641 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct     
     8701 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata     
     8761 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca     
     8821 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga     
     8881 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga     
     8941 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg aaaaaggatc ttcacctaga     
     9001 tccttttcac gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct     
     9061 actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg     
     9121 ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc     
     9181 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct     
     9241 cgccgccaag gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat     
     9301 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga     
     9361 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc     
     9421 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga     
     9481 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg     
     9541 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc     
     9601 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg     
     9661 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga     
     9721 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc     
     9781 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca     
     9841 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg     
     9901 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct     
     9961 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg     
    10021 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc     
    10081 gccttcttga cgagttcttc tgaattttgt taaaattttt gttaaatcag ctcatttttt     
    10141 aaccaatagg ccgaaatcgg caacatccct tataaatcaa aagaatagac cgcgataggg     
    10201 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc     
    10261 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc acccaaatca     
    10321 agttttttgc ggtcgaggtg ccgtaaagct ctaaatcgga accctaaagg gagcccccga     
    10381 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa     
    10441 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc     
    10501 gcgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggatcg aattaattct     
    10561 taattaacat catcaataat atacctt                                         
//