pNV-sgRNA-GFP

                            LOCUS       dna                      4535 bp                                   
FEATURES             Location/Qualifiers
     Reporter        239..398
                     /gene="lacZ_a reporter"
     misc_binding    396..401
                     /dbxref="REBASE:EcoRI"
     misc_binding    396..401
                     /dbxref="REBASE:ApoI"
     misc_binding    402..407
                     /dbxref="REBASE:SacI"
     misc_binding    408..413
                     /dbxref="REBASE:KpnI"
     misc_binding    414..419
                     /dbxref="REBASE:NruI"
     misc_binding    425..430
                     /dbxref="REBASE:XbaI"
     misc_binding    956..961
                     /dbxref="REBASE:NcoI"
     Reporter        958..1674
                     /gene="EGFP reporter"
     ORF             958..1677
                     /sequence="ORF_1 rf(1)"
     misc_binding    1678..1683
                     /dbxref="REBASE:XhoI"
     misc_binding    2260..2265
                     /dbxref="REBASE:BamHI"
     misc_binding    2264..2269
                     /dbxref="REBASE:SmaI"
     misc_binding    2264..2269
                     /dbxref="REBASE:XmaI"
     misc_binding    2267..2272
                     /dbxref="REBASE:ApaI"
     misc_binding    2273..2278
                     /dbxref="REBASE:HincII"
     misc_binding    2273..2278
                     /dbxref="REBASE:AccI"
     misc_binding    2278..2283
                     /dbxref="REBASE:PstI"
     misc_binding    2296..2301
                     /dbxref="REBASE:HindIII"
     Promoter        2363..2392
                     /gene="lac prom"
     Rep_Origin      2701..3320
                     /gene="pBR322 origin"
     Marker          3475..4335
                     /gene="amp marker"
     ORF             3475..4335
                     /sequence="ORF_2 rf(6)"
     Promoter        4377..4405
                     /gene="amp prom"

       1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg     
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc     
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc     
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat     
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt     
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa     
      421 tgcatctaga tatccctagg gggtagggga ggcgcttttc ccaaggcagt ctggagcatg     
      481 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca     
      541 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc     
      601 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac     
      661 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca     
      721 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg     
      781 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg     
      841 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc     
      901 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg gctagcacct cgccaccatg     
      961 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc     
     1021 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc     
     1081 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc     
     1141 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag     
     1201 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc     
     1261 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg     
     1321 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag     
     1381 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc     
     1441 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac     
     1501 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac     
     1561 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg     
     1621 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaactc     
     1681 gagctgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga     
     1741 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt     
     1801 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg     
     1861 attgggaaga gaatagcagg catgctgggg acaattggag ggcctatttc ccatgattcc     
     1921 ttcatatttg catatacgat acaaggctgt tagagagata attagaatta atttgactgt     
     1981 aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt     
     2041 tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta     
     2101 tttcgatttc ttgggtttat atatcttgtg gaaaggacga aacaccggag tcttcaccgg     
     2161 tgaagacgcg ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact     
     2221 tgaaaaagtg gcaccgagtc ggtgcttttt tttgatatcg gatcccgggc ccgtcgactg     
     2281 cagaggcctg catgcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg     
     2341 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg     
     2401 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc     
     2461 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt     
     2521 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct     
     2581 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga     
     2641 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc     
     2701 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg     
     2761 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg     
     2821 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt     
     2881 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt     
     2941 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg     
     3001 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact     
     3061 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt     
     3121 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct     
     3181 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac     
     3241 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc     
     3301 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg     
     3361 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta     
     3421 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca     
     3481 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc     
     3541 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc     
     3601 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc     
     3661 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat     
     3721 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt     
     3781 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc     
     3841 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag     
     3901 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt     
     3961 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac     
     4021 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg     
     4081 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat     
     4141 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc     
     4201 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc     
     4261 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa     
     4321 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg     
     4381 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg     
     4441 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac     
     4501 ctataaaaat aggcgtatca cgaggccctt tcgtc