pPB-N-His
| Vector Name | pPB-N-His |
| VectorType | Protein Vector |
| Antibiotic Information | Bacterial: Kanamycin |
| Sequencing Primers | T7 promoter primer: 5'-TAATACGACTCACTATAGGG-3' T7 terminator primer: 5'-GCTAGTTATTGCTCAGCGG-3' |
| Additional Information | Original Vector: 7023bp Vector without Insert Cassette: 5368bp |
Double-click on the features for more information
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac
1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa
1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
5041 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
5101 agcagcggcc tggtgccgcg cggcagccat atggctagct acatcacaag tttgtacaaa
5161 aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
5221 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg gcggccgcat
5281 taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg attttgagtt
5341 aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
5401 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
5461 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
5521 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
5581 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg
5641 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
5701 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
5761 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
5821 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
5881 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
5941 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
6001 tacaacagta ctgcgatgag tggcagggcg gggcgtaaac gcgtggatcc ggcttactaa
6061 aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg
6121 atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta ttacagtgac
6181 agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg
6241 gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa
6301 aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac
6361 gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta
6421 tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat
6481 ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt
6541 gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc
6601 cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat
6661 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg
6721 tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca
6781 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt
6841 acaaagtggt gatgtaatga ctcgagcacc accaccacca ccactgagat ccggctgcta
6901 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
6961 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
7021 gat
LOCUS dna 7023 bp
FEATURES Location/Qualifiers
Rep_Origin 29..335
/gene="f1 origin"
Marker 560..1375
/gene="kan2 marker"
ORF 560..1375
/sequence="ORF_3 rf(6)"
misc_binding 1249..1254
/dbxref="REBASE:ClaI"
misc_binding 1284..1289
/dbxref="REBASE:NruI"
Rep_Origin 1481..2100
/gene="pBR322 origin"
Other Gene 2515..2706
/gene="ROP other"
misc_binding 3179..3189
/dbxref="REBASE:BglI"
Regulatory_Seq 3515..4606
/gene="lacI reg"
ORF 3515..4474
/sequence="ORF_2 rf(6)"
misc_binding 3738..3743
/dbxref="REBASE:HpaI"
misc_binding 3794..3799
/dbxref="REBASE:EcoRV"
misc_binding 4035..4040
/dbxref="REBASE:ApaI"
misc_binding 4228..4233
/dbxref="REBASE:BclI"
misc_binding 4771..4776
/dbxref="REBASE:SphI"
misc_binding 4964..4969
/dbxref="REBASE:BglII"
Promoter 4984..5002
/gene="T7 prom"
Regulatory_Seq 5002..5029
/gene="lacO reg"
misc_binding 5030..5035
/dbxref="REBASE:XbaI"
misc_binding 5128..5133
/dbxref="REBASE:NdeI"
misc_binding 5271..5278
/dbxref="REBASE:NotI"
Marker 5379..6038
/gene="CAT marker"
ORF 5379..6038
/sequence="ORF_1 rf(3)"
misc_binding 5592..5597
/dbxref="REBASE:EcoRI"
Other Gene 6380..6685
/gene="ccdB other"
misc_binding 6714..6719
/dbxref="REBASE:PstI"
misc_binding 6861..6866
/dbxref="REBASE:XhoI"
Terminator 6895..7023
/gene="T7 term"