pPB-N-His-MBP
| Vector Name | pPB-N-His-MBP |
| VectorType | Protein Vector |
| Antibiotic Information | Bacterial: Kanamycin. |
| Sequencing Primers | MBP Forward primer: 5'-CGCAGATGTCCGCTTTCTGG-3' T7 terminator primer: 5'-GCTAGTTATTGCTCAGCGG-3' |
| Additional Information | No Additional Information |
Double-click on the features for more information
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac
1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa
1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
5041 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
5101 ggtaccaaaa ctgaagaagg taaactggta atctggatta acggcgataa aggctataac
5161 ggtctcgctg aagtcggtaa gaaattcgag aaagataccg gaattaaagt caccgttgag
5221 catccggata aactggaaga gaaattccca caggttgcgg caactggcga tggccctgac
5281 attatcttct gggcacacga ccgctttggt ggctacgctc aatctggcct gttggctgaa
5341 atcaccccgg acaaagcgtt ccaggacaag ctgtatccgt ttacctggga tgccgtacgt
5401 tacaacggca agctgattgc ttacccgatc gctgttgaag cgttatcgct gatttataac
5461 aaagatctgc tgccgaaccc gccaaaaacc tgggaagaga tcccggcgct ggataaagaa
5521 ctgaaagcga aaggtaagag cgcgctgatg ttcaacctgc aagaaccgta cttcacctgg
5581 ccgctgattg ctgctgacgg gggttatgcg ttcaagtatg aaaacggcaa gtacgacatt
5641 aaagacgtgg gcgtggataa cgctggcgcg aaagcgggtc tgaccttcct ggttgacctg
5701 attaaaaaca aacacatgaa tgcagacacc gattactcca tcgcagaagc tgcctttaat
5761 aaaggcgaaa cagcgatgac catcaacggc ccgtgggcat ggtccaacat cgacaccagc
5821 aaagtgaatt atggtgtaac ggtactgccg accttcaagg gtcaaccatc caaaccgttc
5881 gttggcgtgc tgagcgcagg tattaacgcc gccagtccga acaaagagct ggcgaaagag
5941 ttcctcgaaa actatctgct gactgatgaa ggtctggaag cggttaataa agacaaaccg
6001 ctgggtgccg tagcgctgaa gtcttacgag gaagagttgg cgaaagatcc acgtattgcc
6061 gccaccatgg aaaacgccca gaaaggtgaa atcatgccga acatcccgca gatgtccgct
6121 ttctggtatg ccgtgcgtac tgcggtgatc aacgccgcca gcggtcgtca gactgtcgat
6181 gaagccctga aagacgcgca gactggtagc ggctctggtt ctggtagcgg ctctgaaaac
6241 ctttacttcc agggcactgc tagcgatatc aacaagtttg tacaaaaaag ctgaacgaga
6301 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
6361 ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc
6421 tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga
6481 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
6541 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
6601 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
6661 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
6721 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
6781 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
6841 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
6901 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
6961 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
7021 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
7081 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
7141 gatgagtggc agggcggggc gtaaagatct ggatccggct tactaaaagc cagataacag
7201 tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga
7261 agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca
7321 gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat
7381 gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat
7441 ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg
7501 gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg
7561 atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg
7621 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg
7681 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag
7741 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
7801 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac
7861 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
7921 atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggttgat
7981 atctgactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag
8041 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
8101 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggat
LOCUS dna 8149 bp
FEATURES Location/Qualifiers
Rep_Origin 29..335
/gene="f1 origin"
Marker 560..1375
/gene="kan2 marker"
ORF 560..1375
/sequence="ORF_4 rf(4)"
misc_binding 1249..1254
/dbxref="REBASE:ClaI"
misc_binding 1284..1289
/dbxref="REBASE:NruI"
Rep_Origin 1481..2100
/gene="pBR322 origin"
Other Gene 2515..2706
/gene="ROP other"
misc_binding 3179..3189
/dbxref="REBASE:BglI"
Regulatory_Seq 3515..4606
/gene="lacI reg"
ORF 3515..4474
/sequence="ORF_3 rf(4)"
misc_binding 3738..3743
/dbxref="REBASE:HpaI"
misc_binding 4035..4040
/dbxref="REBASE:ApaI"
misc_binding 4771..4776
/dbxref="REBASE:SphI"
Promoter 4984..5002
/gene="T7 prom"
Regulatory_Seq 5002..5029
/gene="lacO reg"
misc_binding 5030..5035
/dbxref="REBASE:XbaI"
ORF 5071..6294
/sequence="ORF_1 rf(1)"
misc_binding 5101..5106
/dbxref="REBASE:KpnI"
Other Gene 6272..6396
/gene="attR1 other"
Other Gene 6296..6396
/gene="attR2 other"
misc_binding 6397..6404
/dbxref="REBASE:NotI"
Marker 6505..7164
/gene="CAT marker"
ORF 6505..7164
/sequence="ORF_2 rf(1)"
misc_binding 6718..6723
/dbxref="REBASE:EcoRI"
Other Gene 7506..7811
/gene="ccdB other"
misc_binding 7840..7845
/dbxref="REBASE:PstI"
Other Gene 7852..7971
/gene="attR1 other"
Other Gene 7852..7952
/gene="attR2 other"
misc_binding 7987..7992
/dbxref="REBASE:XhoI"
Terminator 8021..8149
/gene="T7 term"