pPM-C-HA
| Vector Name | pPM-C-HA |
| VectorType | Protein Vector |
| Antibiotic Information | Kanamycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | Original Vector: 6470bp Vector without Insert Cassette: 4765bp |
Double-click on the features for more information
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc 541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta 601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa 661 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg 721 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc 781 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa 841 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 901 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 961 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 1021 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt 1081 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg 1141 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg 1201 gaaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat 1261 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag 1321 agatctccct atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc 1381 ctggagacgc catccacgct gttttgacct ccatagaagc tagcgatatc aacaagtttg 1441 tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt 1501 ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc actatggcgg 1561 ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt 1621 tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca 1681 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc 1741 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa 1801 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc 1861 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg 1921 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct 1981 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt 2041 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct 2101 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact 2161 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc 2221 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta 2281 atgaattaca acagtactgc gatgagtggc agggcggggc gtaaagatct ggatccggct 2341 tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat 2401 atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac 2461 agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc 2521 ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa 2581 gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt 2641 gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag 2701 ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat 2761 ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc 2821 ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc 2881 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa 2941 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc 3001 tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt 3061 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt 3121 tcttgtacaa agtggttgat atcctcgagt acccatacga cgtcccagac tacgcttgag 3181 tttaaaccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgttgcccg 3241 ggcgcgatcg ctgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 3301 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 3361 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg 3421 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagcaga tcgatctgca 3481 tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt atgggtatta tgggtctgca 3541 ttaatgaatc ggccaacgga tcccggtgtg aaataccgca cagatgcgta aggagaaaat 3601 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 3661 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 3721 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 3781 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 3841 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 3901 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 3961 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 4021 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 4081 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 4141 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 4201 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 4261 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 4321 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 4381 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 4441 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 4501 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 4561 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 4621 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 4681 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 4741 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 4801 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 4861 ttgaaaaagg atcttcacct agatcctttt cacgtagaaa gccagtccgc agaaacggtg 4921 ctgaccccgg atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa 4981 gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg 5041 gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg 5101 caaagtaaac tggatggctt tctcgccgcc aaggatctga tggcgcaggg gatcaagctc 5161 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg 5221 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg 5281 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa 5341 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct 5401 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga 5461 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc 5521 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac 5581 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc 5641 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact 5701 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga 5761 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg 5821 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga 5881 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga 5941 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaattt tgttaaaatt 6001 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaacatc ccttataaat 6061 caaaagaata gaccgcgata gggttgagtg ttgttccagt ttggaacaag agtccactat 6121 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac 6181 tacgtgaacc atcacccaaa tcaagttttt tgcggtcgag gtgccgtaaa gctctaaatc 6241 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga 6301 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca 6361 cgctgcgcgt aaccaccaca cccgcgcgct taatgcgccg ctacagggcg cgtccattcg 6421 ccattcagga tcgaattaat tcttaattaa catcatcaat aatatacctt
LOCUS dna 6470 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 691..696 /dbxref="REBASE:NruI" misc_binding 968..973 /dbxref="REBASE:NdeI" Regulatory_Seq 1305..1344 /gene="tetO reg" misc_binding 1557..1564 /dbxref="REBASE:NotI" Marker 1665..2324 /gene="CAT marker" ORF 1665..2324 /sequence="ORF_2 rf(3)" misc_binding 1878..1883 /dbxref="REBASE:EcoRI" Other Gene 2666..2971 /gene="ccdB other" misc_binding 3000..3005 /dbxref="REBASE:PstI" misc_binding 3144..3149 /dbxref="REBASE:XhoI" misc_binding 3470..3475 /dbxref="REBASE:ClaI" Rep_Origin 3782..4401 /gene="pBR322 origin" Promoter 5054..5103 /gene="NEOKAN prom" ORF 5192..5986 /sequence="ORF_1 rf(2)" Marker 5195..5983 /gene="NTP_II marker" Rep_Origin 6091..6396 /gene="f1 origin"