pPM-C-His
| Vector Name | pPM-C-His |
| VectorType | Protein Vector |
| Antibiotic Information | Kanamycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | Original Vector: 6459bp Vector without Insert Cassette: 4752bp |
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***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
ORIGIN
1 TTTTGGATTG AAGCCAATAT GATAATGAGG GGGTGGAGTT TGTGACGTGG
51 CGCGGGGCGT GGGAACGGGG CGGGTGACGT AGTAGTGTGG CGGAAGTGTG
101 ATGTTGCAAG TGTGGCGGAA CACATGTAAG CGACGGATGT GGCAAAAGTG
151 ACGTTTTTGG TGTGCGCCGG TGTACACAGG AAGTGACAAT TTTCGCGCGG
201 TTTTAGGCGG ATGTTGTAGT AAATTTGGGC GTAACCGAGT AAGATTTGGC
251 CATTTTCGCG GGAAAACTGA ATAAGAGGAA GTGAAATCTG AATAATTTTG
301 TGTTACTCAT AGCGCGTAAT ACGGCAGACC TCAGCGCTAG ATTATTGAAG
351 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT
401 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
451 CCTGACGTTA ACTATAACGG TCCTAAGGTA GCGAAAGCTC AGATCTGGAT
501 CTCCCGATCC CCTATGGTCG ACTCTCAGTA CAATCTGCTC TGATGCCGCA
551 TAGTTAAGCC AGTATCTGCT CCCTGCTTGT GTGTTGGAGG TCGCTGAGTA
601 GTGCGCGAGC AAAATTTAAG CTACAACAAG GCAAGGCTTG ACCGACAATT
651 GCATGAAGAA TCTGCTTAGG GTTAGGCGTT TTGCGCTGCT TCGCGATGTA
701 CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA
751 ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA
801 CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC
851 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC
901 TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG
951 CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT
1001 GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA
1051 CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG
1101 TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA
1151 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG
1201 GAACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT
1251 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA
1301 GCTCTCCCTA TCAGTGATAG AGATCTCCCT ATCAGTGATA GAGATCGTCG
1351 ACGAGCTCGT TTAGTGAACC GTCAGATCGC CTGGAGACGC CATCCACGCT
1401 GTTTTGACCT CCATAGAGCT AGCGATATCA ACAAGTTTGT ACAAAAAAGC
1451 TGAACGAGAA ACGTAAAATG ATATAAATAT CAATATATTA AATTAGATTT
1501 TGCATAAAAA ACAGACTACA TAATACTGTA AAACACAACA TATCCAGTCA
1551 CTATGGCGGC CGCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC
1601 TCGTATAATG TGTGGATTTT GAGTTAGGAT CCGTCGAGAT TTTCAGGAGC
1651 TAAGGAAGCT AAAATGGAGA AAAAAATCAC TGGATATACC ACCGTTGATA
1701 TATCCCAATG GCATCGTAAA GAACATTTTG AGGCATTTCA GTCAGTTGCT
1751 CAATGTACCT ATAACCAGAC CGTTCAGCTG GATATTACGG CCTTTTTAAA
1801 GACCGTAAAG AAAAATAAGC ACAAGTTTTA TCCGGCCTTT ATTCACATTC
1851 TTGCCCGCCT GATGAATGCT CATCCGGAAT TCCGTATGGC AATGAAAGAC
1901 GGTGAGCTGG TGATATGGGA TAGTGTTCAC CCTTGTTACA CCGTTTTCCA
1951 TGAGCAAACT GAAACGTTTT CATCGCTCTG GAGTGAATAC CACGACGATT
2001 TCCGGCAGTT TCTACACATA TATTCGCAAG ATGTGGCGTG TTACGGTGAA
2051 AACCTGGCCT ATTTCCCTAA AGGGTTTATT GAGAATATGT TTTTCGTCTC
2101 AGCCAATCCC TGGGTGAGTT TCACCAGTTT TGATTTAAAC GTGGCCAATA
2151 TGGACAACTT CTTCGCCCCC GTTTTCACCA TGGGCAAATA TTATACGCAA
2201 GGCGACAAGG TGCTGATGCC GCTGGCGATT CAGGTTCATC ATGCCGTTTG
2251 TGATGGCTTC CATGTCGGCA GAATGCTTAA TGAATTACAA CAGTACTGCG
2301 ATGAGTGGCA GGGCGGGGCG TAAAGATCTG GATCCGGCTT ACTAAAAGCC
2351 AGATAACAGT ATGCGTATTT GCGCGCTGAT TTTTGCGGTA TAAGAATATA
2401 TACTGATATG TATACCCGAA GTATGTCAAA AAGAGGTATG CTATGAAGCA
2451 GCGTATTACA GTGACAGTTG ACAGCGACAG CTATCAGTTG CTCAAGGCAT
2501 ATATGATGTC AATATCTCCG GTCTGGTAAG CACAACCATG CAGAATGAAG
2551 CCCGTCGTCT GCGTGCCGAA CGCTGGAAAG CGGAAAATCA GGAAGGGATG
2601 GCTGAGGTCG CCCGGTTTAT TGAAATGAAC GGCTCTTTTG CTGACGAGAA
2651 CAGGGGCTGG TGAAATGCAG TTTAAGGTTT ACACCTATAA AAGAGAGAGC
2701 CGTTATCGTC TGTTTGTGGA TGTACAGAGT GATATTATTG ACACGCCCGG
2751 GCGACGGATG GTGATCCCCC TGGCCAGTGC ACGTCTGCTG TCAGATAAAG
2801 TCTCCCGTGA ACTTTACCCG GTGGTGCATA TCGGGGATGA AAGCTGGCGC
2851 ATGATGACCA CCGATATGGC CAGTGTGCCG GTCTCCGTTA TCGGGGAAGA
2901 AGTGGCTGAT CTCAGCCACC GCGAAAATGA CATCAAAAAC GCCATTAACC
2951 TGATGTTCTG GGGAATATAA ATGTCAGGCT CCCTTATACA CAGCCAGTCT
3001 GCAGGTCGAC CATAGTGACT GGATATGTTG TGTTTTACAG TATTATGTAG
3051 TCTGTTTTTT ATGCAAAATC TAATTTAATA TATTGATATT TATATCATTT
3101 TACGTTTCTC GTTCAGCTTT CTTGTACAAA GTGGTTGATA TCCTCGAGCA
3151 TCATCACCAT CACCATTGAG TTTAAACCGC TGATCAGCCT CGACTGTGCC
3201 TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA
3251 CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT
3301 GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG
3351 GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG
3401 ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AAAGAACCAG CAGATCGATC
3451 TGCATCTATG TCGGGTGCGG AGAAAGAGGT AATGAAATGG CATTATGGGT
3501 ATTATGGGTC TGCATTAATG AATCGGCCAA CGGATCCATA TGCGGTGTGA
3551 AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCTCTTCCG
3601 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG
3651 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA
3701 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC
3751 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC
3801 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
3851 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC
3901 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG
3951 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT
4001 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC
4051 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA
4101 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
4151 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT
4201 ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA
4251 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
4301 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA
4351 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT
4401 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA
4451 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA
4501 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC
4551 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC
4601 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG
4651 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT
4701 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC
4751 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA
4801 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGAAAAAGGA
4851 TCTTCACCTA GATCCTTTTC ACGTAGAAAG CCAGTCCGCA GAAACGGTGC
4901 TGACCCCGGA TGAATGTCAG CTACTGGGCT ATCTGGACAA GGGAAAACGC
4951 AAGCGCAAAG AGAAAGCAGG TAGCTTGCAG TGGGCTTACA TGGCGATAGC
5001 TAGACTGGGC GGTTTTATGG ACAGCAAGCG AACCGGAATT GCCAGCTGGG
5051 GCGCCCTCTG GTAAGGTTGG GAAGCCCTGC AAAGTAAACT GGATGGCTTT
5101 CTCGCCGCCA AGGATCTGAT GGCGCAGGGG ATCAAGCTCT GATCAAGAGA
5151 CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT
5201 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA
5251 GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC
5301 GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG
5351 CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
5401 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT
5451 TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC
5501 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA
5551 TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG
5601 CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA
5651 GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG
5701 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC
5751 CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC
5801 CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA
5851 TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT
5901 ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT
5951 GACGAGTTCT TCTGAATTTT GTTAAAATTT TTGTTAAATC AGCTCATTTT
6001 TTAACCAATA GGCCGAAATC GGCAACATCC CTTATAAATC AAAAGAATAG
6051 ACCGCGATAG GGTTGAGTGT TGTTCCAGTT TGGAACAAGA GTCCACTATT
6101 AAAGAACGTG GACTCCAACG TCAAAGGGCG AAAAACCGTC TATCAGGGCG
6151 ATGGCCCACT ACGTGAACCA TCACCCAAAT CAAGTTTTTT GCGGTCGAGG
6201 TGCCGTAAAG CTCTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC
6251 TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA
6301 AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA
6351 ACCACCACAC CCGCGCGCTT AATGCGCCGC TACAGGGCGC GTCCATTCGC
6401 CATTCAGGAT CGAATTAATT CTTAATTAAC ATCATCAATA ATATACCTT
//
LOCUS dna 6459 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 691..696
/dbxref="REBASE:NruI"
misc_binding 968..973
/dbxref="REBASE:NdeI"
Regulatory_Seq 1305..1344
/gene="tetO reg"
misc_binding 1557..1564
/dbxref="REBASE:NotI"
Marker 1665..2324
/gene="CAT marker"
ORF 1665..2324
/sequence="ORF_1 rf(3)"
misc_binding 1878..1883
/dbxref="REBASE:EcoRI"
Other Gene 2666..2971
/gene="ccdB other"
misc_binding 3000..3005
/dbxref="REBASE:PstI"
misc_binding 3144..3149
/dbxref="REBASE:XhoI"
Tag 3150..3167
/gene="6xHis tag"
misc_binding 3459..3464
/dbxref="REBASE:ClaI"
Rep_Origin 3771..4390
/gene="pBR322 origin"
Promoter 5043..5092
/gene="NEOKAN prom"
ORF 5181..5975
/sequence="ORF_2 rf(3)"
Marker 5184..5972
/gene="NTP_II marker"
Rep_Origin 6080..6385
/gene="f1 origin"