pPM-N-D-C-HA

Vector Name pPM-N-D-C-HA
VectorType Protein Vector
Antibiotic Information Kanamycin
Sequencing Primers CMV sequencing primer
5`---CGC AAA TGG GCG GTA GGC GTG---3`
Additional Information No Additional Information
Double-click on the features for more information
***Note: The vector sequence shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page. The restriction sites within the cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc
541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
661 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
721 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
781 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
841 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
901 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
961 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
1021 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
1081 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
1141 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
1201 gaaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
1261 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag
1321 agatctccct atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc
1381 ctggagacgc catccacgct gttttgacct ccatagagct agcatggact acaaggacga
1441 cgatgacaag gactacaagg acgacgatga caaggactac aaggacgacg atgacaagag
1501 cgaaaacctg tacttccagg gagaattcga tatcctgcag agcggccgct ctagagggcc
1561 cctcgagtac ccatacgacg tcccagacta cgcttgagtt taaaccgctg atcagcctcg
1621 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
1681 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
1741 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
1801 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
1861 agaaccagca gatcgatctc tatgtcgggt gcggagaaag aggtaatgaa atggcattat
1921 gggtattatg ggtctgcatt aatgaatcgg ccaaggatcc atatgcggtg tgaaataccg
1981 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
2041 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
2101 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
2161 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
2221 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
2281 gataccaggc gtttccccct gaagctccct cgtgcgctct cctgttccga ccctgccgct
2341 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
2401 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
2461 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
2521 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
2581 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
2641 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
2701 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
2761 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
2821 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
2881 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
2941 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
3001 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
3061 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
3121 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
3181 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
3241 taatagtttg cgcaacgttg ttgaaaaagg atcttcacct agatcctttt cacgtagaaa
3301 gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc tatctggaca
3361 agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac atggcgatag
3421 ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg ggcgccctct
3481 ggtaaggttg ggaagccctg caaagtaaac tggatggctt tctcgccgcc aaggatctga
3541 tggcgcaggg gatcaagctc tgatcaagag acaggatgag gatcgtttcg catgattgaa
3601 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
3661 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
3721 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
3781 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
3841 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
3901 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
3961 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
4021 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
4081 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
4141 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
4201 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
4261 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
4321 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
4381 ttctgaattt tgttaaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
4441 cggcaacatc ccttataaat caaaagaata gaccgcgata gggttgagtg ttgttccagt
4501 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
4561 ctatcagggc gatggcccac tacgtgaacc atcacccaaa tcaagttttt tgcggtcgag
4621 gtgccgtaaa gctctaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
4681 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
4741 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgcgcgct taatgcgccg
4801 ctacagggcg cgtccattcg ccattcagga tcgaattaat tcttaattaa catcatcaat
4861 aatatacctt
                            LOCUS       dna                      4870 bp                                   
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 691..696
/dbxref="REBASE:NruI"
Regulatory_Seq 1305..1344
/gene="tetO reg"
misc_binding 1523..1528
/dbxref="REBASE:EcoRI"
misc_binding 1529..1534
/dbxref="REBASE:EcoRV"
misc_binding 1535..1540
/dbxref="REBASE:PstI"
misc_binding 1542..1549
/dbxref="REBASE:NotI"
misc_binding 1550..1555
/dbxref="REBASE:XbaI"
misc_binding 1556..1561
/dbxref="REBASE:ApaI"
misc_binding 1562..1567
/dbxref="REBASE:AvrI"
misc_binding 1562..1567
/dbxref="REBASE:XhoI"
misc_binding 1562..1567
/dbxref="REBASE:AvaI"
misc_binding 1872..1877
/dbxref="REBASE:ClaI"
misc_binding 1955..1960
/dbxref="REBASE:BamHI"
Rep_Origin 2184..2801
/gene="pBR322 origin"
Promoter 3454..3503
/gene="NEOKAN prom"
ORF 3592..4386
/sequence="ORF_1 rf(1)"
Marker 3595..4383
/gene="NTP_II marker"
Rep_Origin 4491..4796
/gene="f1 origin"