pPM-N-D-C-His

                            LOCUS       dna                      4862 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    457..462
                     /dbxref="REBASE:HpaI"
     misc_binding    691..696
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  1305..1344
                     /gene="tetO reg"
     misc_binding    1523..1528
                     /dbxref="REBASE:EcoRI"
     misc_binding    1529..1534
                     /dbxref="REBASE:EcoRV"
     misc_binding    1535..1540
                     /dbxref="REBASE:PstI"
     misc_binding    1542..1549
                     /dbxref="REBASE:NotI"
     misc_binding    1550..1555
                     /dbxref="REBASE:XbaI"
     misc_binding    1556..1561
                     /dbxref="REBASE:ApaI"
     misc_binding    1561..1566
                     /dbxref="REBASE:AvrI"
     misc_binding    1561..1566
                     /dbxref="REBASE:XhoI"
     misc_binding    1561..1566
                     /dbxref="REBASE:AvaI"
     Tag             1567..1584
                     /gene="6xHis tag"
     misc_binding    1862..1867
                     /dbxref="REBASE:ClaI"
     misc_binding    1945..1950
                     /dbxref="REBASE:BamHI"
     Rep_Origin      2174..2793
                     /gene="pBR322 origin"
     Promoter        3446..3495
                     /gene="NEOKAN prom"
     ORF             3584..4378
                     /sequence="ORF_1 rf(2)"
     Marker          3587..4375
                     /gene="NTP_II marker"
     Rep_Origin      4483..4788
                     /gene="f1 origin"
BASE COUNT    1204 a   1177 c   1327 g   1154 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta     
      481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc     
      541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta     
      601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa     
      661 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg     
      721 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc     
      781 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa     
      841 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac     
      901 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca     
      961 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg     
     1021 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt     
     1081 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg     
     1141 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg     
     1201 gaaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat     
     1261 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag     
     1321 agatctccct atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc     
     1381 ctggagacgc catccacgct gttttgacct ccatagagct agcatggact acaaggacga     
     1441 cgatgacaag gactacaagg acgacgatga caaggactac aaggacgacg atgacaagag     
     1501 cgaaaacctg tacttccagg gagaattcga tatcctgcag agcggccgct ctagagggcc     
     1561 ctcgagcatc atcaccatca ccattgagtt taaaccgctg atcagcctcg actgtgcctt     
     1621 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg     
     1681 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt     
     1741 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca     
     1801 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagca     
     1861 gatcgatctc tatgtcgggt gcggagaaag aggtaatgaa atggcattat gggtattatg     
     1921 ggtctgcatt aatgaatcgg ccaaggatcc atatgcggtg tgaaataccg cacagatgcg     
     1981 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct     
     2041 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca     
     2101 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga     
     2161 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc     
     2221 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg     
     2281 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat     
     2341 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt     
     2401 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc     
     2461 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg     
     2521 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg     
     2581 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg     
     2641 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg     
     2701 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca     
     2761 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga     
     2821 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga     
     2881 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt     
     2941 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt     
     3001 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat     
     3061 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag     
     3121 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct     
     3181 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt     
     3241 tgcgcaacgt tgttgaaaaa ggatcttcac ctagatcctt ttcacgtaga aagccagtcc     
     3301 gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa     
     3361 cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg     
     3421 ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt     
     3481 tgggaagccc tgcaaagtaa actggatggc tttctcgccg ccaaggatct gatggcgcag     
     3541 gggatcaagc tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg     
     3601 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca     
     3661 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt     
     3721 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg     
     3781 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga     
     3841 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca     
     3901 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct     
     3961 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac     
     4021 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc     
     4081 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt     
     4141 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt     
     4201 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg     
     4261 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat     
     4321 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaat     
     4381 tttgttaaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaca     
     4441 tcccttataa atcaaaagaa tagaccgcga tagggttgag tgttgttcca gtttggaaca     
     4501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg     
     4561 gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttgcggtcg aggtgccgta     
     4621 aagctctaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg     
     4681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa     
     4741 gtgtagcggt cacgctgcgc gtaaccacca cacccgcgcg cttaatgcgc cgctacaggg     
     4801 cgcgtccatt cgccattcag gatcgaatta attcttaatt aacatcatca ataatatacc     
     4861 tt                                                                    
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