pRetro-E2 SV40

                            LOCUS       dna                     11535 bp                                   
FEATURES             Location/Qualifiers
     Promoter        216..418
                     /gene="SV40 prom"
     Rep_Origin      263..340
                     /gene="SV40 origin"
     ORF             472..1266
                     /sequence="ORF_1 rf(1)"
     Marker          475..1263
                     /gene="NTP_II marker"
     Other Gene      1368..1919
                     /gene="5_LTR2 other"
     misc_binding    1623..1628
                     /dbxref="REBASE:XbaI"
     Rep_Origin      2441..3060
                     /gene="pBR322 origin"
     Marker          3215..4075
                     /gene="amp marker"
     ORF             3215..4075
                     /sequence="ORF_5 rf(6)"
     misc_binding    3402..3412
                     /dbxref="REBASE:BglI"
     Promoter        4117..4145
                     /gene="amp prom"
     Other Gene      5113..5922
                     /gene="psi_plus_pack other"
     ORF             6091..7149
                     /sequence="ORF_3 rf(4)"
     Other Gene      7154..7184
                     /gene="SV40_late_19s_int other"
     Regulatory_Seq  7438..7674
                     /gene="SV40ER reg"
     Promoter        7581..7783
                     /gene="SV40 prom"
     Rep_Origin      7628..7705
                     /gene="SV40 origin"
     Other Gene      8316..8331
                     /gene="SV40_int other"
     Other Gene      8395..10263
                     /gene="SV40_lrgT other"
     ORF             8461..10263
                     /sequence="ORF_2 rf(1)"
     Terminator      10179..10420
                     /gene="SV40_PA term"
     ORF             10361..11122
                     /sequence="ORF_4 rf(6)"
     misc_binding    10691..10696
                     /dbxref="REBASE:ApaI"
     Regulatory_Seq  11190..11426
                     /gene="SV40ER reg"
     Promoter        11333..11535
                     /gene="SV40 prom"
     Rep_Origin      11380..11457
                     /gene="SV40 origin"
BASE COUNT    3043 a   2766 c   2719 g   3007 t    0 others
ORIGIN
        1 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag     
       61 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc     
      121 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc     
      181 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat     
      241 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc     
      301 gccccatggc tgactaattt tttttattta tgcagaggcc gagcctcgag gggcctctga     
      361 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg     
      421 gctgcaggtc gaggcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa     
      481 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac     
      541 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg     
      601 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag     
      661 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt     
      721 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg     
      781 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg     
      841 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga     
      901 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag     
      961 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat     
     1021 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt     
     1081 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg     
     1141 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt     
     1201 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc     
     1261 ttctgagcgg gactctgggg ttcgataaaa taaaagattt tatttagtct ccagaaaaag     
     1321 gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc     
     1381 aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat     
     1441 ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca     
     1501 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt     
     1561 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag     
     1621 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc     
     1681 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga     
     1741 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc     
     1801 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc     
     1861 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt     
     1921 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg     
     1981 taagctggct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc     
     2041 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc     
     2101 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc     
     2161 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata     
     2221 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg     
     2281 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc     
     2341 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt     
     2401 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc     
     2461 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa     
     2521 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc     
     2581 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg     
     2641 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc     
     2701 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc     
     2761 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca     
     2821 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact     
     2881 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg     
     2941 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt     
     3001 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct     
     3061 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga     
     3121 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa     
     3181 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac     
     3241 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga     
     3301 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc     
     3361 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca     
     3421 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta     
     3481 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg     
     3541 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc     
     3601 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg     
     3661 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt     
     3721 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt     
     3781 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata     
     3841 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc     
     3901 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac     
     3961 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa     
     4021 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct     
     4081 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat     
     4141 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc     
     4201 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca     
     4261 cgaggccctt tcgtcttcaa gaattgctag caattgctag caattgctag caattcatac     
     4321 cagatcaccg aaaactgtcc tccaaatgtg tccccctcac actcccaaat tcgcgggctt     
     4381 ctgcctctta gaccactcta ccctattccc cacactcacc ggagccaaag ccgcggccct     
     4441 tccgtttctt tgcttttgaa agaccccacc cgtaggtggc aagctagctt aagtaacgcc     
     4501 actttgcaag gcatggaaaa atacataact gagaatagaa aagttcagat caaggtcagg     
     4561 aacaaagaaa cagctgaata ccaaacagga tatctgtggt aagcggttcc tgccccggct     
     4621 cagggccaag aacagatgag acagctgagt gatgggccaa acaggatatc tgtggtaagc     
     4681 agttcctgcc ccggctcggg gccaagaaca gatggtcccc agatgcggtc cagccctcag     
     4741 cagtttctag tgaatcatca gatgtttcca gggtgcccca aggacctgaa aatgaccctg     
     4801 taccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttccgctct     
     4861 ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcttc cgatagactg     
     4921 cgtcgcccgg gtacccgtat tcccaataaa gcctcttgct gtttgcatcc gaatcgtggt     
     4981 ctcgctgttc cttgggaggg tctcctctga gtgattgact acccacgacg ggggtctttc     
     5041 atttgggggc tcgtccggga tttggagacc cctgcccagg gaccaccgac ccaccaccgg     
     5101 gaggtaagct ggccagcaac ttatctgtgt ctgtccgatt gtctagtgtc tatgtttgat     
     5161 gttatgcgcc tgcgtctgta ctagttagct aactagctct gtatctggcg gacccgtggt     
     5221 ggaactgacg agttctgaac acccggccgc aaccctggga gacgtcccag ggactttggg     
     5281 ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga     
     5341 tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg     
     5401 ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg     
     5461 tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa attagggcca gactgttacc     
     5521 actcccttaa gtttgacctt aggtcactgg aaagatgtcg agcggatcgc tcacaaccag     
     5581 tcggtagatg tcaagaagag acgttgggtt accttctgct ctgcagaatg gccaaccttt     
     5641 aacgtcggat ggccgcgaga cggcaccttt aaccgagacc tcatcaccca ggttaagatc     
     5701 aaggtctttt cacctggccc gcatggacac ccagaccagg tcccctacat cgtgacctgg     
     5761 gaagccttgg cttttgaccc ccctccctgg gtcaagccct ttgtacaccc taagcctccg     
     5821 cctcctcttc ctccatccgc cccgtctctc ccccttgaac ctcctcgttc gaccccgcct     
     5881 cgatcctccc tttatccagc cctcactcct tctctaggcg ccggaattct agccacactg     
     5941 tagcaaggca gttgttcttt gtctggagag tcatctgtaa actgtttttc agctgctaag     
     6001 cttttactta agcctttttg atgttcatca ggattgccca tttgaggatt taaaaagcac     
     6061 tccacctcag tgaagctgtc tactccagtt ttaactccta gaacttctat tcctcctttt     
     6121 atgacgagct ttggcacttg cactggttcc tttggttttt tgggagctgc ccctggacaa     
     6181 cttccttttc tttttgttgg ggccatcttc ataagctttt agagcagaag taacacttcc     
     6241 gtacaggcct agaagtaaag gcaacatcca ctgaggagca gttctttgat ttgcaccacc     
     6301 aggagcctca aatttttcaa taaattcacc tgactgcaca ttaggacttt ggctttgagc     
     6361 ttcccacctc tcagttactt gctgaatact gtctgcttca tcaatattat cataggtgtg     
     6421 cccaaatgat atttgcaacc cttccctgtt ggctacttgt ctcaccattg taggcctaat     
     6481 gggagacaaa gtagagtagt aatcttgtaa agagttatac caattaacag gagcattaat     
     6541 tactgtccaa gtagtttcct ctaaaaacct tgccaaactg tcccttaaat atctttgggt     
     6601 tcttctttca agctcctgtg aggtgagcct aggaatgtca ttttgtatta cacgccaaaa     
     6661 agcttgagaa atggcattaa aaagtgttgg accccaatgt ctggggtcaa gatactgaac     
     6721 actgtgaaca aaggtttgta ctccaggaaa taaaatatca taataatcat ctggcctata     
     6781 caaatctaca gccattcctg gttgttgata taaaccaaca gtagaaactt tgtgatccca     
     6841 gtcactaaaa aatctatacc ccacttgagc aacagcgctc acaccagtca cagtttgcag     
     6901 taaagctgca aatccagcta tagcagcagg agccccagat atcacagcat aggcctgtgg     
     6961 agtgaggcct atagcagcaa ttgcctcaga ggttgttagg ccttcaacag tagcaacaga     
     7021 tgcaagttgc acttcaattg cagcagcggc ctctccagca gcaatttcag ctactgaaaa     
     7081 tccagtagca gcagcagctt cagacacagt agcaattagg tcccccaaca gtgttaaagc     
     7141 agcacccatg gacctgaaat aaaagacaaa aagactaaac ttaccagtta actttctggt     
     7201 ttttcagtta acctttctgg ttttttgcgt ttcccgtcaa cagtatcttc cccttcacaa     
     7261 aattgcagca aaagctctaa aacaaacaca aaaaggcgtt gagctgtttt tttactttca     
     7321 gtccatgacc tacgaacctt aacggaggcc tggcgtgaca gccggcgcag caccatggcc     
     7381 tgaaataacc tctgaaagag gaacttggtt aggtaccttc tgaggcggaa agaaccagct     
     7441 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat     
     7501 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc     
     7561 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac     
     7621 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact     
     7681 aatttttttt atttatgcag aggccgagcc tcgaggggcc tctgagctat tccagaagta     
     7741 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctttgcaaag atggataaag     
     7801 ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtgcct     
     7861 gggggaatat tcctctgatg agaaaggcat atttaaaaaa atgcaaggag tttcatcctg     
     7921 ataaaggagg agatgaagaa aaaatgaaga aaatgaatac tctgtacaag aaaatggaag     
     7981 atggagtaaa atatgctcat caacctgact ttggaggctt ctgggatgca actgaggtat     
     8041 ttgcttcttc cttaaatcct ggtgttgatg caatgtactg caaacaatgg cctgagtgtg     
     8101 caaagaaaat gtctgctaac tgcatatgct tgctgtgctt actgaggatg aagcatgaaa     
     8161 atagaaaatt atacaggaaa gatccacttg tgtgggttga ttgctactgc ttcgattgct     
     8221 ttagaatgtg gtttggactt gatctttgtg aaggaacctt acttctgtgg tgtgacataa     
     8281 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta     
     8341 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact     
     8401 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa     
     8461 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag     
     8521 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat     
     8581 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct     
     8641 gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac     
     8701 agttataatc ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt     
     8761 aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag     
     8821 gaatatttga tgtatagtgc cttgactaga gatccatttt ctgttattga ggaaagtttg     
     8881 ccaggtgggt taaaggagca tgattttaat ccagaagaag cagaggaaac taaacaagtg     
     8941 tcctggaagc ttgtaacaga gtatgcaatg gaaacaaaat gtgatgatgt gttgttattg     
     9001 cttgggatgt acttggaatt tcagtacagt tttgaaatgt gtttaaaatg tattaaaaaa     
     9061 gaacagccca gccactataa gtaccatgaa aagcattatg caaatgctgc tatatttgct     
     9121 gacagcaaaa accaaaaaac catatgccaa caggctgttg atactgtttt agctaaaaag     
     9181 cgggttgata gcctacaatt aactagagaa caaatgttaa caaacagatt taatgatctt     
     9241 ttggatagga tggatataat gtttggttct acaggctctg ctgacataga agaatggatg     
     9301 gctggagttg cttggctaca ctgtttgttg cccaaaatgg attcagtggt gtatgacttt     
     9361 ttaaaatgca tggtgtacaa cattcctaaa aaaagatact ggctgtttaa aggaccaatt     
     9421 gatagtggta aaactacatt agcagctgct ttgcttgaat tatgtggggg gaaagcttta     
     9481 aatgttaatt tgcccttgga caggctgaac tttgagctag gagtagctat tgaccagttt     
     9541 ttagtagttt ttgaggatgt aaagggcact ggaggggagt ccagagattt gccttcaggt     
     9601 cagggaatta ataacctgga caatttaagg gattatttgg atggcagtgt taaggtaaac     
     9661 ttagaaaaga aacacctaaa taaaagaact caaatatttc cccctggaat agtcaccatg     
     9721 aatgagtaca gtgtgcctaa aacactgcag gccagatttg taaaacaaat agattttagg     
     9781 cccaaagatt atttaaagca ttgcctggaa cgcagtgagt ttttgttaga aaagagaata     
     9841 attcaaagtg gcattgcttt gcttcttatg ttaatttggt acagacctgt ggctgagttt     
     9901 gctcaaagta ttcagagcag aattgtggag tggaaagaga gattggacaa agagtttagt     
     9961 ttgtcagtgt atcaaaaaat gaagtttaat gtggctatgg gaattggagt tttagattgg     
    10021 ctaagaaaca gtgatgatga tgatgaagac agccaggaaa atgctgataa aaatgaagat     
    10081 ggtggggaga agaacatgga agactcaggg catgaaacag gcattgattc acagtcccaa     
    10141 ggctcatttc aggcccctca gtcctcacag tctgttcatg atcataatca gccataccac     
    10201 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca     
    10261 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata     
    10321 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg     
    10381 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cccaggaagc tcctctgtgt     
    10441 cctcataaac cctaacctcc tctacttgag aggacattcc aatcataggc tgcccatcca     
    10501 ccctctgtgt cctcctgtta attaggtcac ttaacaaaaa ggaaattggg taggggtttt     
    10561 tcacagaccg ctttctaagg gtaattttaa aatatctggg aagtcccttc cactgctgtg     
    10621 ttccagaagt gttggtaaac agcccacaaa tgtcaacagc agaaacatac aagctgtcag     
    10681 ctttgcacaa gggcccaaca ccctgctcat caagaagcac tgtggttgct gtgttagtaa     
    10741 tgtgcaaaac aggaggcaca ttttccccac ctgtgtaggt tccaaaatat ctagtgtttt     
    10801 catttttact tggatcagga acccagcact ccactggata agcattatcc ttatccaaaa     
    10861 cagccttgtg gtcagtgttc atctgctgac tgtcaactgt agcatttttt ggggttacag     
    10921 tttgagcagg atatttggtc ctgtagtttg ctaacacacc ctgcagctcc aaaggttccc     
    10981 caccaacagc aaaaaaatga aaatttgacc cttgaatggg ttttccagca ccattttcat     
    11041 gagttttttg tgtccctgaa tgcaagttta acatagcagt taccccaata acctcagttt     
    11101 taacagtaac agcttcccac atcaaaatat ttccacaggt taagtcctca tttaaattag     
    11161 gcaaaggaat tcgttaactc gaggatccgg ctgtggaatg tgtgtcagtt agggtgtgga     
    11221 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca     
    11281 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc     
    11341 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc     
    11401 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag     
    11461 cctcgagggg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc     
    11521 ttttgcaaaa agctt                                                      
//