pRetroG-CMV
Vector Name | pRetroG-CMV |
VectorType | Retroviral Plasmid |
Antibiotic Information | Bacterial: Ampicillin Mammalian: Puromycin |
Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
Additional Information | The gene expression is driven by a CMV promoter, without any Tag or reporter.
Original Vector: 7999bp Vector without Insert Cassette: 6294bp. |
Double-click on the features for more information
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat 61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc 121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca 181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg 241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa 301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac 361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa 421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac 481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg 541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt 601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc 661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg 721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt 781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg 841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt 901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa 961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct 1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt 1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa 1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc 1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc 1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt 1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc 1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta 1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc 1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac 1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg 1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc 1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg 2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct 2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga 2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac 2881 cggactcaga tctaagcttc ctagcgatat caacaagttt gtacaaaaaa gctgaacgag 2941 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta 3001 cataatactg taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg 3061 ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag 3121 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga 3181 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac 3241 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa 3301 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga 3361 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta 3421 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga 3481 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc 3541 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag 3601 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac 3661 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca 3721 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg 3781 cgatgagtgg cagggcgggg cgtaaagatc tggatccggc ttactaaaag ccagataaca 3841 gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg 3901 aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac 3961 agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca 4021 tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga 4081 tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct 4141 ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg 4201 gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt 4261 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat 4321 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa 4381 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc 4441 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga 4501 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa 4561 tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggttga 4621 tatctgactc gaggtttaaa cgaattcgtt aggccattaa ggcctgtcga caagcggccg 4681 cctcggccaa acatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat 4741 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg 4801 gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc 4861 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag 4921 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc 4981 cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga 5041 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga 5101 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata 5161 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt 5221 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt 5281 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat ttgggggctc 5341 gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg 5401 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 5461 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 5521 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta 5581 ctgataactt cgtataatgt atgctatacg aagttattag gtctgaagag gagtttacgt 5641 ccagccaagc tagggccgcg atccggaacc cttaatataa cttcgtataa tgtatgctat 5701 acgaagttat cagtactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca 5761 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc 5821 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 5881 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 5941 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 6001 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 6061 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 6121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 6181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 6241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 6301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 6361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 6421 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 6481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 6541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 6601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 6661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 6721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 6781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 6841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 6901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 6961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 7021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg 7081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 7141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 7201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 7261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 7321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg 7381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 7441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 7501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 7561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 7621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 7681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 7741 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 7801 tatcacgagg ccctttcgtc ttcaagaatt gctagcaatt gctagcaatt gctagcaatt 7861 cataccagat caccgaaaac tgtcctccaa atgtgtcccc ctcacactcc caaattcgcg 7921 ggcttctgcc tcttagacca ctctacccta ttccccacac tcaccggagc caaagccgcg 7981 gcccttccgt ttctttgct
LOCUS dna 7999 bp FEATURES Location/Qualifiers Other Gene 659..1468 /gene="psi_plus_pack other" ORF 1449..2216 /sequence="ORF_3 rf(6)" Marker 1566..2165 /gene="puro marker" Promoter 2787..2868 /gene="CMV prom" misc_binding 2894..2899 /dbxref="REBASE:HindIII" Other Gene 2913..3037 /gene="attR1 other" Other Gene 2937..3037 /gene="attR2 other" Marker 3146..3805 /gene="CAT marker" ORF 3146..3805 /sequence="ORF_1 rf(2)" Other Gene 4147..4452 /gene="ccdB other" Other Gene 4493..4612 /gene="attR1 other" Other Gene 4493..4593 /gene="attR2 other" misc_binding 4628..4633 /dbxref="REBASE:XhoI" misc_binding 4693..4698 /dbxref="REBASE:ClaI" Other Gene 4780..5331 /gene="5_LTR2 other" misc_binding 5035..5040 /dbxref="REBASE:XbaI" Other Gene 5584..5617 /gene="loxP other" Other Gene 5677..5710 /gene="loxP other" Rep_Origin 5986..6605 /gene="pBR322 origin" Marker 6760..7620 /gene="amp marker" ORF 6760..7620 /sequence="ORF_2 rf(5)" Promoter 7662..7690 /gene="amp prom" BASE COUNT 1931 a 2156 c 2005 g 1907 t 0 others ORIGIN 1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat 61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc 121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca 181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg 241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa 301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac 361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa 421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac 481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg 541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt 601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc 661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg 721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt 781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg 841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt 901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa 961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct 1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt 1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa 1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc 1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc 1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt 1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc 1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta 1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc 1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac 1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg 1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc 1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg 2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct 2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga 2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac 2881 cggactcaga tctaagcttc ctagcgatat caacaagttt gtacaaaaaa gctgaacgag 2941 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta 3001 cataatactg taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg 3061 ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag 3121 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga 3181 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac 3241 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa 3301 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga 3361 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta 3421 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga 3481 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc 3541 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag 3601 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac 3661 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca 3721 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg 3781 cgatgagtgg cagggcgggg cgtaaagatc tggatccggc ttactaaaag ccagataaca 3841 gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg 3901 aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac 3961 agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca 4021 tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga 4081 tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct 4141 ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg 4201 gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt 4261 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat 4321 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa 4381 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc 4441 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga 4501 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa 4561 tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggttga 4621 tatctgactc gaggtttaaa cgaattcgtt aggccattaa ggcctgtcga caagcggccg 4681 cctcggccaa acatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat 4741 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg 4801 gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc 4861 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag 4921 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc 4981 cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga 5041 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga 5101 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata 5161 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt 5221 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt 5281 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat ttgggggctc 5341 gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg 5401 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 5461 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 5521 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta 5581 ctgataactt cgtataatgt atgctatacg aagttattag gtctgaagag gagtttacgt 5641 ccagccaagc tagggccgcg atccggaacc cttaatataa cttcgtataa tgtatgctat 5701 acgaagttat cagtactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca 5761 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc 5821 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 5881 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 5941 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 6001 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 6061 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 6121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 6181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 6241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 6301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 6361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 6421 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 6481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 6541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 6601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 6661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 6721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 6781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 6841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 6901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 6961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 7021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg 7081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 7141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 7201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 7261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 7321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg 7381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 7441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 7501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 7561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 7621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 7681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 7741 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 7801 tatcacgagg ccctttcgtc ttcaagaatt gctagcaatt gctagcaatt gctagcaatt 7861 cataccagat caccgaaaac tgtcctccaa atgtgtcccc ctcacactcc caaattcgcg 7921 ggcttctgcc tcttagacca ctctacccta ttccccacac tcaccggagc caaagccgcg 7981 gcccttccgt ttctttgct //