pRetroG-CMV

Vector Name pRetroG-CMV
VectorType Retroviral Plasmid
Antibiotic Information Bacterial: Ampicillin
Mammalian: Puromycin
Sequencing Primers CMV sequencing primer
5`---CGC AAA TGG GCG GTA GGC GTG---3`
Additional Information The gene expression is driven by a CMV promoter, without any Tag or reporter.
Original Vector: 7999bp
Vector without Insert Cassette: 6294bp.
Double-click on the features for more information
***Note: The vector sequence shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page. The restriction sites within the cloning region may differ from the provided sequence.
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat     
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc     
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca     
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg     
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa     
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac     
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa     
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac     
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg     
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt     
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc     
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg     
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt     
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg     
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt     
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa     
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct     
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt     
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa     
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc     
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc     
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt     
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc     
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta     
     1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc     
     1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac     
     1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg     
     1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc     
     1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc     
     1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc     
     1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga     
     1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca     
     1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc     
     1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct     
     2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca     
     2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg     
     2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct     
     2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga     
     2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc     
     2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg     
     2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa     
     2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca     
     2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac     
     2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc     
     2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga     
     2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg     
     2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta     
     2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac     
     2881 cggactcaga tctaagcttc ctagcgatat caacaagttt gtacaaaaaa gctgaacgag     
     2941 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta     
     3001 cataatactg taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg     
     3061 ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag     
     3121 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga     
     3181 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac     
     3241 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa     
     3301 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga     
     3361 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta     
     3421 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga     
     3481 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc     
     3541 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag     
     3601 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac     
     3661 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca     
     3721 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg     
     3781 cgatgagtgg cagggcgggg cgtaaagatc tggatccggc ttactaaaag ccagataaca     
     3841 gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg     
     3901 aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac     
     3961 agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca     
     4021 tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga     
     4081 tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct     
     4141 ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg     
     4201 gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt     
     4261 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat     
     4321 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa     
     4381 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc     
     4441 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga     
     4501 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa     
     4561 tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggttga     
     4621 tatctgactc gaggtttaaa cgaattcgtt aggccattaa ggcctgtcga caagcggccg     
     4681 cctcggccaa acatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat     
     4741 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg     
     4801 gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc     
     4861 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag     
     4921 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc     
     4981 cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga     
     5041 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga     
     5101 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata     
     5161 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt     
     5221 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt     
     5281 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat ttgggggctc     
     5341 gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg     
     5401 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac     
     5461 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc     
     5521 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta     
     5581 ctgataactt cgtataatgt atgctatacg aagttattag gtctgaagag gagtttacgt     
     5641 ccagccaagc tagggccgcg atccggaacc cttaatataa cttcgtataa tgtatgctat     
     5701 acgaagttat cagtactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca     
     5761 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc     
     5821 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc     
     5881 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa     
     5941 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt     
     6001 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg     
     6061 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg     
     6121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag     
     6181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc     
     6241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa     
     6301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg     
     6361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc     
     6421 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac     
     6481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg     
     6541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt     
     6601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt     
     6661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa     
     6721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga     
     6781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt     
     6841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg     
     6901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga     
     6961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga     
     7021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg     
     7081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc     
     7141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc     
     7201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca     
     7261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac     
     7321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg     
     7381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc     
     7441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg     
     7501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac     
     7561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat     
     7621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata     
     7681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa     
     7741 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg     
     7801 tatcacgagg ccctttcgtc ttcaagaatt gctagcaatt gctagcaatt gctagcaatt     
     7861 cataccagat caccgaaaac tgtcctccaa atgtgtcccc ctcacactcc caaattcgcg     
     7921 ggcttctgcc tcttagacca ctctacccta ttccccacac tcaccggagc caaagccgcg     
     7981 gcccttccgt ttctttgct   
                            LOCUS       dna                      7999 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      659..1468
                     /gene="psi_plus_pack other"
     ORF             1449..2216
                     /sequence="ORF_3 rf(6)"
     Marker          1566..2165
                     /gene="puro marker"
     Promoter        2787..2868
                     /gene="CMV prom"
     misc_binding    2894..2899
                     /dbxref="REBASE:HindIII"
     Other Gene      2913..3037
                     /gene="attR1 other"
     Other Gene      2937..3037
                     /gene="attR2 other"
     Marker          3146..3805
                     /gene="CAT marker"
     ORF             3146..3805
                     /sequence="ORF_1 rf(2)"
     Other Gene      4147..4452
                     /gene="ccdB other"
     Other Gene      4493..4612
                     /gene="attR1 other"
     Other Gene      4493..4593
                     /gene="attR2 other"
     misc_binding    4628..4633
                     /dbxref="REBASE:XhoI"
     misc_binding    4693..4698
                     /dbxref="REBASE:ClaI"
     Other Gene      4780..5331
                     /gene="5_LTR2 other"
     misc_binding    5035..5040
                     /dbxref="REBASE:XbaI"
     Other Gene      5584..5617
                     /gene="loxP other"
     Other Gene      5677..5710
                     /gene="loxP other"
     Rep_Origin      5986..6605
                     /gene="pBR322 origin"
     Marker          6760..7620
                     /gene="amp marker"
     ORF             6760..7620
                     /sequence="ORF_2 rf(5)"
     Promoter        7662..7690
                     /gene="amp prom"
BASE COUNT    1931 a   2156 c   2005 g   1907 t    0 others
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat     
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc     
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca     
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg     
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa     
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac     
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa     
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac     
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg     
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt     
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc     
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg     
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt     
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg     
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt     
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa     
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct     
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt     
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa     
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc     
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc     
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt     
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc     
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta     
     1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc     
     1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac     
     1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg     
     1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc     
     1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc     
     1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc     
     1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga     
     1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca     
     1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc     
     1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct     
     2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca     
     2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg     
     2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct     
     2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga     
     2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc     
     2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg     
     2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa     
     2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca     
     2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac     
     2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc     
     2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga     
     2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg     
     2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta     
     2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac     
     2881 cggactcaga tctaagcttc ctagcgatat caacaagttt gtacaaaaaa gctgaacgag     
     2941 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta     
     3001 cataatactg taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg     
     3061 ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag     
     3121 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga     
     3181 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac     
     3241 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa     
     3301 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga     
     3361 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta     
     3421 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga     
     3481 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc     
     3541 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag     
     3601 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac     
     3661 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca     
     3721 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg     
     3781 cgatgagtgg cagggcgggg cgtaaagatc tggatccggc ttactaaaag ccagataaca     
     3841 gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg     
     3901 aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac     
     3961 agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca     
     4021 tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga     
     4081 tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct     
     4141 ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg     
     4201 gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt     
     4261 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat     
     4321 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa     
     4381 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc     
     4441 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga     
     4501 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa     
     4561 tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggttga     
     4621 tatctgactc gaggtttaaa cgaattcgtt aggccattaa ggcctgtcga caagcggccg     
     4681 cctcggccaa acatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat     
     4741 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg     
     4801 gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc     
     4861 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag     
     4921 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc     
     4981 cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga     
     5041 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga     
     5101 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata     
     5161 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt     
     5221 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt     
     5281 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat ttgggggctc     
     5341 gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg     
     5401 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac     
     5461 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc     
     5521 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta     
     5581 ctgataactt cgtataatgt atgctatacg aagttattag gtctgaagag gagtttacgt     
     5641 ccagccaagc tagggccgcg atccggaacc cttaatataa cttcgtataa tgtatgctat     
     5701 acgaagttat cagtactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca     
     5761 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc     
     5821 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc     
     5881 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa     
     5941 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt     
     6001 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg     
     6061 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg     
     6121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag     
     6181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc     
     6241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa     
     6301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg     
     6361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc     
     6421 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac     
     6481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg     
     6541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt     
     6601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt     
     6661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa     
     6721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga     
     6781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt     
     6841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg     
     6901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga     
     6961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga     
     7021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg     
     7081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc     
     7141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc     
     7201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca     
     7261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac     
     7321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg     
     7381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc     
     7441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg     
     7501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac     
     7561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat     
     7621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata     
     7681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa     
     7741 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg     
     7801 tatcacgagg ccctttcgtc ttcaagaatt gctagcaatt gctagcaatt gctagcaatt     
     7861 cataccagat caccgaaaac tgtcctccaa atgtgtcccc ctcacactcc caaattcgcg     
     7921 ggcttctgcc tcttagacca ctctacccta ttccccacac tcaccggagc caaagccgcg     
     7981 gcccttccgt ttctttgct                                                  
//