pRetroG-CMV-GFP-stop

                            LOCUS       dna                      6980 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      659..1468
                     /gene="psi_plus_pack other"
     ORF             1460..2215
                     /sequence="ORF_3 rf(5)"
     Marker          1565..2164
                     /gene="puro marker"
     Promoter        2786..2867
                     /gene="CMV prom"
     misc_binding    2887..2892
                     /dbxref="REBASE:BglII"
     misc_binding    2891..2896
                     /dbxref="REBASE:XhoI"
     Reporter        2903..3619
                     /gene="EGFP reporter"
     ORF             2903..3622
                     /sequence="ORF_1 rf(2)"
     misc_binding    3623..3628
                     /dbxref="REBASE:EcoRI"
     misc_binding    3655..3662
                     /dbxref="REBASE:NotI"
     misc_binding    3674..3679
                     /dbxref="REBASE:ClaI"
     Other Gene      3761..4312
                     /gene="5_LTR2 other"
     misc_binding    4016..4021
                     /dbxref="REBASE:XbaI"
     Other Gene      4565..4598
                     /gene="loxP other"
     Other Gene      4658..4691
                     /gene="loxP other"
     Rep_Origin      4967..5586
                     /gene="pBR322 origin"
     Marker          5741..6601
                     /gene="amp marker"
     ORF             5741..6601
                     /sequence="ORF_2 rf(5)"
     Promoter        6643..6671
                     /gene="amp prom"
BASE COUNT    1613 a   2046 c   1802 g   1519 t    0 others
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat     
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc     
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca     
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg     
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa     
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac     
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa     
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac     
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg     
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt     
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc     
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg     
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt     
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg     
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt     
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa     
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct     
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt     
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa     
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc     
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc     
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt     
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc     
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta     
     1441 tccagccctc actccttctc taggcgccgg aatgatcccc cgggctgcag gtcggccgcc     
     1501 acgaccggtg ccgccaccat cccctgaccc acgcccctga cccctcacaa ggagacgacc     
     1561 ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc     
     1621 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc     
     1681 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct     
     1741 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc     
     1801 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag     
     1861 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa     
     1921 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct     
     1981 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt     
     2041 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac     
     2101 cgccgacgtc gagtgcccga aggaccgcgc gacctggtgc atgacccgca agcccggtgc     
     2161 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatggctc     
     2221 cgaccgaagc cacccggggc ggccccgccg accccgcacc cgcccccgag gcccaccgac     
     2281 tctagtaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg     
     2341 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga     
     2401 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat     
     2461 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa     
     2521 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca     
     2581 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca     
     2641 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat     
     2701 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg     
     2761 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac     
     2821 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc     
     2881 ggactcagat ctcgaggcca ccatggtgag caagggcgag gagctgttca ccggggtggt     
     2941 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga     
     3001 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa     
     3061 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag     
     3121 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta     
     3181 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt     
     3241 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga     
     3301 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat     
     3361 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga     
     3421 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc     
     3481 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa     
     3541 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg     
     3601 catggacgag ctgtacaagt aagaattcgt taggccatta aggcctgtcg acaagcggcc     
     3661 gcctcggcca aacatcgata aaataaaaga ttttatttag tctccagaaa aaggggggaa     
     3721 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat     
     3781 ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag     
     3841 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa     
     3901 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc     
     3961 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag     
     4021 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg     
     4081 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat     
     4141 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg     
     4201 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct     
     4261 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct     
     4321 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg     
     4381 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga     
     4441 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag     
     4501 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt     
     4561 actgataact tcgtataatg tatgctatac gaagttatta ggtctgaaga ggagtttacg     
     4621 tccagccaag ctagggccgc gatccggaac ccttaatata acttcgtata atgtatgcta     
     4681 tacgaagtta tcagtactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc     
     4741 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct     
     4801 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat     
     4861 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga     
     4921 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt     
     4981 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt     
     5041 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc     
     5101 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa     
     5161 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct     
     5221 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta     
     5281 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg     
     5341 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc     
     5401 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta     
     5461 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg     
     5521 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt     
     5581 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg     
     5641 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta     
     5701 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg     
     5761 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg     
     5821 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc     
     5881 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg     
     5941 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg     
     6001 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag     
     6061 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat     
     6121 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc     
     6181 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc     
     6241 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa     
     6301 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac     
     6361 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt     
     6421 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc     
     6481 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa     
     6541 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca     
     6601 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat     
     6661 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa     
     6721 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc     
     6781 gtatcacgag gccctttcgt cttcaagaat tgctagcaat tgctagcaat tgctagcaat     
     6841 tcataccaga tcaccgaaaa ctgtcctcca aatgtgtccc cctcacactc ccaaattcgc     
     6901 gggcttctgc ctcttagacc actctaccct attccccaca ctcaccggag ccaaagccgc     
     6961 ggcccttccg tttctttgct                                                 
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